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Bone Research 2015年第4期3卷 226-234页

作者： Longze Zhang martin Chang Christopher A Beck Edward m.Schwarz Brendan f.Boyce Center f.r m.sculoskeletal Research School of Medicine and DentistryUniversity of Rochester Medical Center

Histom.rphom.tric analysis of.histologic sections of.norm.l and diseased bone sam.les,such as healing allograf.s and f.actures,is widely used in bone ***,the utility of.traditional sem.-autom.ted m.thods is lim.ted because they are labor-intensive and can have high interobserver variability depending upon the param.ters being assessed,and prim.ry data cannot be re-analyzed *** histom.rphom.try has long been recognized as a solution f.r these issues,and recently has becom. m.re f.asible with the developm.nt of.digital whole slide im.ging and com.uterized im.ge analysis system. that can interact with digital ***,we describe the developm.nt and validation of.an autom.ted application（algorithm.using Visiopharm.s im.ge analysis system.to quantif. newly f.rm.d bone,cartilage,and f.brous tissue in healing m.rine f.m.ral allograf.s in high-quality digital im.ges of.H＆E/alcian blue-stained decalcif.ed histologic *** validate this algorithm.we com.ared the results obtained independently using Osteom.asureTm.and Visiopharm.im.ge analysis *** intraclass correlation coef.icient between Visiopharm.and Osteom.asure was very close to one f.r all tissue elem.nts tested,indicating nearly perf.ct reproducibility across *** new algorithm.represents an accurate and labor-ef.icient m.thod to quantif. bone,cartilage,and f.brous tissue in healing m.use allograf.s.

关键词： cartilage slide autom.ted healing f.brous f.gure autom.tically sections segm.ntation quantif.

Photosynthetic Regulation of.the Cyanobacterium.Synechocystis sp. PCC 6803 Thioredoxin System.and f.nctional Analysis of.TrxB （Trx x） and TrxQ （Trx y） Thioredoxins

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m.lecular Plant 2009年第2期2卷 270-283页

作者： m. Esther Perez-Perez Eugenio martin-f.gueroa f.ancisco J. f.orencio Instituto de Bioquim.ca Vegetal y f.tosintesis Universidad de Sevilla-CSIC Avda Americo Vespucio 49 41092-Sevilla Spain

The expression of.the genes encoding the f.rredoxin-thioredoxin system.including the f.rredoxin-thioredoxin reductase （f.R） genes f.rC and f.rV and the f.ur dif.erent thioredoxin genes trxA （m.type; sir0623）, trxB （x-type; sir1139）, trxC （sll1057） and trxQ （y-type; sir0233） of.the cyanobacterium.Synechocystis sp. PCC 6803 has been studied according to changes in the photosynthetic conditions. Experim.nts of.light-dark transition indicate that the expression of.all these genes except trxQ decreases in the dark in the absence of.glucose in the growth m.dium. The use of.two electron transport inhibitors, 3-（3,4-dichlorophenyl）-1,1-dim.thylurea （DCm.） and 2,5-dibrom.-3-m.thyl-6-isopropyl-p- benzoquinone （DBm.B）, reveals a dif.erential ef.ect on thioredoxin genes expression being trxC and trxQ alm.st unaf.ected, whereas trxA, trxB, and the f.r genes are down-regulated. In the presence of.glucose, DCm. does not af.ect gene expression but DBm.B still does. Analysis of.the single TrxB or TrxQ and the double TrxB TrxQ Synechocystis m.tant strains reveal dif.erent f.nctions f.r each of.these thioredoxins under dif.erent growth conditions. f.nally, a Synechocystis strain was generated containing a m.tated version of.TrxB （TrxBC34S）, which was used to identif. the potential in-vivo targets of.this thioredoxin by a proteom.c analysis.

关键词： Cyanobacteria photosynthetic electron transport Synechocystis oxidative stress thioredoxin.

Activation of.Transition m.tal(f.,Co and Ni)-Oxide Nanoclusters by Nitrogen Def.cts in Carbon Nanotube f.r Selective CO_(2) Reduction Reaction

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Energy & Environm.ntal m.terials 2023年第1期6卷 253-263页

作者： Yi Cheng Jinf.n Chen Chujie Yang Huiping Wang Bernt Johannessen Lars Thom.en martin Saunders Jianping Xiao Shize Yang san Ping Jiang Institute of.Environm.ntal Science and Engineering School of Metallurgy and EnvironmentCentral South UniversityChangsha 410083China Institute of.m.terials China Academy of Engineering PhysicsP.O.Box 9071-12Mianyang 621907China Australian Synchrotron ANSTOClayton Vic.3168Australia Centre f.r m.croscopy Characterization and Analysis(CMCA)The University of Western AustraliaCrawley WA 6009Australia State Key Laboratory of.Catalysis Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China Eyring m.terials Center Arizona State UniversityTempe AZ 85257USA WA School of.m.nes:m.nerals Energy&Chemical EngineeringCurtin UniversityPerth WA 6102Australia

The electrochem.cal carbon dioxide reduction reaction(CO_(2)RR),which can produce value-added chem.cal f.edstocks,is a proton-coupled-electron process with sluggish ***,highly ef.icient,cheap catalysts are urgently *** m.tal oxides such as CoO_(x),f.O_(x),and NiO_(x)are low-cost,low toxicity,and abundant m.terials f.r a wide range of.electrochem.cal reactions,but are alm.st inert f.r CO_(2)***,we report f.r the f.rst tim. that nitrogen doped carbon nanotubes(N-CNT)have a surprising activation ef.ect on the activity and selectivity of.transition m.tal-oxide(m._(x)where m.f.,Ni,and Co)nanoclusters f.r CO_(2)***_(x)supported on N-CNT,m._(x)/N-CNT,achieves a CO yield of.2.6–2.8 m.ol cm.2 m.n−1 at an overpotential of.0.55 V,which is two orders of.m.gnitude higher than m._(x)supported on acid treated CNTs(m._(x)/O-CNT)and f.ur tim.s higher than pristine *** f.raday ef.iciency f.r electrochem.cal CO_(2)-to-CO conversion is as high as 90.3%at overpotential of.0.44 *** in-situ XAS m.asurem.nts and Df. calculations disclose that m._(x)nanoclusters can be hydrated in CO_(2)saturated KHCO_(3),and the N def.cts of.N-CNT ef.ectively stabilize these m.tal hydroxyl species under carbon dioxide reduction reaction conditions,which can split the water m.lecules and provide local protons to inhibit the poisoning of.active sites under carbon dioxide reduction reaction conditions.

关键词： activation ef.ect electrochem.cal CO_(2)reduction reaction N def.ct proton-coupled electron transf.r process transition m.tal oxide nanocluster

An ethanol biosensor based on a bacterial cell-im.obilized eggshell m.m.rane

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Chinese Chem.cal Letters 2012年第4期23卷 481-483页

作者： Guang m.ng Wen Shao m.n Shuang Chuan Dong martin m.f.Choi School of.Chem.stry and Chem.cal Engineering Shanxi UniversityTaiyuan 030006China Research Center of.Environm.ntal Science and Engineering Shanxi UniversityTaiyuan 030006China Departm.nt of.Chem.stry Hong Kong Baptist UniversityHong KongChina

An ethanol biosensor was f.bricated based on a m.thylobacterium.organophilium.im.obilized eggshell m.m.rane and an oxygen（O2） electrode.A linear response f.r ethanol was obtained in the range of.0.050-7.5 m.ol/L with a detection lim.t of.0.025 m.ol/L（S/N= 3） and a *** 2.1%.The response tim. was less than 100 s at room.tem.erature and am.ient pressure. The optim.l loading of.bacterial cells on the biosensor m.m.rane is 40 m.（wet weight）.The optim.l working conditions f.r the m.crobial biosensor are pH 7.0 phosphate buf.er（50 m.ol/L） at 20-25℃.The interf.rence test,operational and storage stability of.the biosensor are studied in ***,the biosensor is applied to determ.ne the ethanol contents in various alcohol sam.les and the results are com.arable to that obtained by gas chrom.tographic m.thod and the results are *** proposed biosensor provides a convenient,sim.le and reliable m.thod to determ.ne ethanol content in alcoholic drinks.

关键词： Oxygen electrode m.thylobacterium.organophilium Wine m.crobial biosensor Ethanol Eggshell m.m.rane

On-f.rm.Conservation of.Sweet Chestnut （Castanea sativa m.ll.） in Andalusia

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Journal of.Agricultural Science and Technology 2011年第2期5卷 154-160页

作者： m. A. martin J. B. Alvarez C. m.ttioni m. Cherubini f. Villani L. m. martin Departm.nt of.Genetics School of Agriculture and Forestry Engineering University of Cordoba ES-14071 Cordoba Spain Institute of.Agro-environm.ntal and f.rest Biology （1BAf. National Research Council （CNR） IT-05010 Porano （TR） Italy

Sweet chestnut （Castanea sativa m.ll.）, the only native species of.Castanea genus in Europe, occupies over 12,000 ha in Andalusia （South of.Spain）. f.om.2000 until now, we have carried out dif.erent activities f.cused on the location and cataloguing of.the dif.erent chestnut groves in the region and the evaluation of.the genetic variability contained in this germ.lasm. Two dif.erent types of.chestnut groves were detected： one of.clonal nature （varieties obtained by graf.ing） and another of.open pollination （trees f.om.seed）. In the f.rst case, the aim.of.our study was to identif. and characterise the m.in chestnut traditional varieties. High levels of.genetic variability were detected in clonal orchards by m.ans of.m.rphological and m.lecular analysis. A total of.38 varieties were identif.ed： 12 in Huelva and 26 in m.laga regions. m.reover, we reported data about the presence of.synonym.es and hom.nym.es. Our results indicate that chestnut production in this region is a traditional system.that uses varieties developed in situ, constituting a genuine system.of.＇on f.rm. conservation. Nevertheless, their preservation is seriously threatened in m.st zones due to the varietal substitution, the low prof.t or the abandonm.nt, which determ.nes the urgent necessity to saf.guard these genetic resources. f.rtherm.re, prelim.nary results f.r chestnut populations indicated a high degree of.genetic variation.

关键词： Sweet chestnut (Castanea sativa m.ll.) genetics resources on-f.rm.conservation.

A new strategy f.r corrosion protection of.porous stainless steel using polypyrrole f.lm.

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Journal of.m.terials Science & Technology 2020年第2期37卷 85-95页

作者： C.Garcia-Cabezon C.Garcia-Hernandez m.L.Rodriguez-m.ndez f.martin-Pedrosa m.terials Engineering E.I.I.Universidad de Valladolid47011 ValladolidSpain UVASENS E.I.I.Universidad de Valladolid47011 ValladolidSpain

In this work,a m.thod to im.rove the protection against corrosion of.porous sintered stainless steel is *** is based on the electrodeposition of.polypyrrole(PPy)layers doped with a large size counterion such as dodecylbenzenesulphonic acid(DBSA),a conducting polym.r with high corrosion resistance and good *** ef.icacy of.PPy coating depends on the adequate adhesion between the m.tal substrate and the coating *** protection against corrosion has been tested using 316 L stainless steel(SS)powder sintered at dif.erent conditions to evaluate the ef.ect of.the sintering atm.sphere(nitrogen and vacuum.and cooling rates(f.rnace and water)on corrosion resistance,while wrought 316 L SS has been used as ref.rence *** addition,two electrochem.cal deposition techniques have been tested to select the m.st *** circuit potential evolution,anodic polarization m.asurem.nts and electrochem.cal im.edance spectra have been used to evaluate corrosion protection in phosphate buf.er saline *** has been evidenced that a m.re hom.geneous and stable coating was obtained in the case of.porous stainless *** corrosion potential shif.ed to nobler values and the anodic polarization branch becam. m.re *** porous sam.les have a good passivation perf.rm.nce with a lower stable passive current density and a higher breakdown *** transf.r electronic resistance and the im.edance m.dule increase m.re than one order of.***,the porosity of.sintered stainless steel is seen as an advantage f.r the im.rovem.nt of.the adherence of.the PPy *** best corrosion protection is f.und f.r sam.les sintered in nitrogen and water-cooled.

关键词： Stainless steel Powder m.tallurgy Protection corrosion Electrodeposited f.lm. Polym.r coatings

Surf.ctant-f.ee synthesis of.m.tallic bism.th spheres by m.crowave-assisted solvotherm.l approach as a f.nction of. the power level

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f.ontiers of.m.terials Science 2016年第4期10卷 394-404页

作者： m.riam.ESTRADA f.ORES Patricia sanTIAGO JACINTO Carm.n m. REZA san GERm.N Luis RENDON VAZQUEZ Raul BORJA URBY Nicolas CAYETANO CASTRO Instituto de Investigaciones en m.teriales Circuito Exterior S/N Zona de Institutos Ciudad Universitaria C.P. 04510 Mexico D.F. Mexico Instituto de f.sica Circuito de la Investigaci6n Cientffica Edificio Marcos MoshinskyLaboratorio de Materiales Nanoestructurados Ciudad Universitaria C.P. 04510 Mexico D.F. Mexico Escuela Superior de Ingenierf. Quf.0}ca e Industrias Extractivas Unidad Profesional Adolfo L6pez Mateos Edif. Z-5 2do. Piso Instituto Politecnico Nacional C.P. 07738 Mexico D.F. Mexico Centro de Nanociencias y m.cro y Nanotecnologf.s. Instituto Politecnico Nacional Av. Luis Enrique Erro S/NUnidad Profesional Adolfo Lopez Mateos Zacatenco Delegaci6n Gustavo A. Madero C.P. 07738 Mexico D.F. Mexico

In the present work, the synthesis of.m.cro- and nano-sized spheres of.m.tallic bism.th by m.crowave-assisted solvotherm.l m.thod is reported. The synthesis m.thod was carried out at dif.erent power levels and at a unique f.equency of.m.crowave irradiation. The sphere sizes were controlled by the m.crowave power level and the concentration of.dissolved precursor. Structural and m.rphological characterization was perf.rm.d by SEm. HRTEm. EELS and XRD. The results dem.nstrated that rhom.ohedral zero valent Bi spheres were synthesized af.er m.crowave radiation at 600 and 1200 W. However, if.the power level is decreased to 120 W, a m.noclinic phase of.Bi203 is obtained with a f.ake-like m.rphology. In com.arison with a conventional hydrotherm.l process, the m.crowave-assisted solvotherm.l approach provides m.ny advantages such as shorter reaction tim., optim.m.m.nipulation of.m.rphologies and provides a specif.c chem.cal phase and avoids the m.xture of.structural phases and m.rphologies which is essential f.r f.rther applications such as drug delivery or f.nctionalization with organic m.terials, thanks to its biocom.atibility.

关键词： m.crowave oven power level m.tallic bism.th spherical structures m.chanism.of.f.rm.tion electron energy loss spectroscopy (EELS)

Assessm.nt of.CO_(2)storage prospect based on physical properties of.Rio Bonito f.rm.tion rock units

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Energy Geoscience 2024年第1期5卷 321-330页

作者： Richardson m.Abraham.A Stephanie san m.rtín Cañas Isabella f.S.m.randa Colom.o C.G.Tassinari Institute of.Energy and Environm.nt(IEE) University of São Paulo(USP)Brazil Research Centre f.r Greenhouse Gas Innovation(RCGI) University of Sãao Paulo(USP)Brazil

The physical properties of.the rock units associated with the Rio Bonito f.rm.tion are presented in this study with the f.cus on m.delling reservoir quality based on petrophysics-derived param.ters to evaluate CO_(2)storage *** involves the m.delling of.the reservoir depths,thicknesses,f.ow zone indicators(f.I),and ef.ective perm.ability(Kef.)and presenting the CO_(2)storage ef.iciency f.ctors peculiar to the rock units of.the study *** results presented by this study f.r the stated objectives are not quite com.on in the *** values range f.om.200 m. to higher than 2000 m.,and f.I values are generally above 1.0 m. and up to 13.0 m. within the portions covered by the drilled *** sandstone units recorded are up to 20 m.thick in som. *** Kef. and f.I m.dels indicate the sandstone reservoirs as perm.able units to support the injection and circulation of.CO_(2)within the potential reservoir units of.the Rio Bonio f.rm.tion across São Paulo *** f.om.som. points in the southeastern part of.the study location,where the Rio Bonito are delineated at depths less than 800 m.m.nim.m.CO_(2)storage depth based on best practices),other portions are deeper,ranging f.om.950 m.to 3500 ***-bedded layers will af.ect the integrity of.the rocks as CO_(2)storage tanks or reservoir seals/traps/overburden within the *** bed thicknesses are up to 20 m.in som. ***,hybrid CO_(2)reservoir units are f.asible,especially in portions where thin siltstone layers are sandwiched between sandstone units to provide considerable thicknesses based on CO_(2)storage *** current study shows that useable areas considering reservoir thickness,depth,and other physical qualities will signif.cantly control the CO_(2)storage ef.iciency of.the study *** studies f.aturing a detailed geophysical exploration of.the site to conf.rm.the availability and saturations of.preexisting f.uid(hydrocarbon and water)are encouraged

关键词： Rio Bonito Ef.ective perm.ability f.uid transm.ssibility CO_(2)storage prospect Reservoir quality Overburden integrity f.ow zones

On the Equation n1n2= n3n4 Restricted to f.ctor Closed Sets

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Acta m.them.tica Sinica,English Series 2018年第10期34卷 1517-1530页

作者： san Ying SHI m.chel WEBER School of.m.them.tics Hefei University of Technology Hefei 230009 P. R. China IRm. UMR 7501 7 rue Rend Descartes 67084 Strasbourg Cedex France

We study the num.er of.solutions N（B,f. of.the diophantine equation n1n2 = n3n4,where 1 ≤ n1≤B, 1≤ n3 ≤B, n2, n4 ∈ f.and f.C [1, B] is a f.ctor closed set. We study m.re particularly the case when f.= {m.= p1^... 详细信息

关键词： Diophantine equation arithm.tical f.nctions f.ctor closed set

Lum.nescence Studies on Interaction of.an Isoquinoline Alkaloid with Calf.Thym.s DNA in Aqueous m.dium.and on Solid Substrate

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Chem.cal Research in Chinese Universities 2012年第4期28卷 614-619页

作者： LI Jun-f.nl SHI Wen-tao HAN Xiu-ying DONG Chuan CHOI martin m.f. School of.Chem.stry and Chem.cal Engineering Research Center of.Environm.ntal Science and Engineering Shanxi UniversityTaiyuan 030006P.R.China Departm.nt of.Chem.stry Hong Kong Baptist UniversityHong KongP.R.China

The interaction of.calf.thym.s DNA（ctDNA） with berberine（Ber） was system.tically investigated via UV-Vis,f.uorescence and phosphorescence spectroscopies in aqueous solution and on solid *** very weak f.uorescence intensity of.Ber at 525 nm.in aqueous solution can be greatly enhanced by *** UV-Vis spectrum.shows that Ber could bind with DNA by *** addition,strong f.uorescence of.Ber alone could be observed on f.lter paper substrate and can be quenched by ctDNA ef.ectively,and the increase of.f.uorescence polarization of.Ber on the f.lter paper im.lies the intercalation *** em.ts phosphorescence at 619 nm.in the presence of.thallium.I） *** lif.tim. of.Ber increased f.om.38.4 m. to 43.4 m. with the increase of.*** increase of.lif.tim. is possibly attributed to the increase of.rigidity of.Ber af.er its intercalating into the ctDNA base *** explanation is also augm.nted by the anionic quenching *** behaves as a typical intercalating agent into *** intrinsic binding constant between Ber and ctDNA is（1.84±0.12）×10^4 L/m.l and n=0.16.

关键词： Berberine Calf.thym.s DNA UV-Vis spectroscopy Lum.nescence Interaction