Luminescence Studies on Interaction of an Isoquinoline Alkaloid with Calf Thymus DNA in Aqueous Medium and on Solid Substrate

作     者：LI Jun-fenl SHI Wen-tao HAN Xiu-ying DONG Chuan CHOI Martin M.F. 

作者机构：School of Chemistry and Chemical Engineering Research Center of Environmental Science and EngineeringShanxi UniversityTaiyuan 030006P.R.China Department of ChemistryHong Kong Baptist UniversityHong KongP.R.China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2012年第28卷第4期

页      面：614-619页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081702[工学-化学工艺] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：Supported by the National Natural Science Foundation of China(Nos.21175086,21105060) the Natural Science Foundation of Shanxi Province,China(No.2010011013-1) the Research Project Supported by Shanxi Scholarship Council,China 

主　　题：Berberine Calf thymus DNA UV-Vis spectroscopy Luminescence Interaction 

摘      要：The interaction of calf thymus DNA（ctDNA） with berberine（Ber） was systematically investigated via UV-Vis,fluorescence and phosphorescence spectroscopies in aqueous solution and on solid *** very weak fluorescence intensity of Ber at 525 nm in aqueous solution can be greatly enhanced by *** UV-Vis spectrum shows that Ber could bind with DNA by *** addition,strong fluorescence of Ber alone could be observed on filter paper substrate and can be quenched by ctDNA effectively,and the increase of fluorescence polarization of Ber on the filter paper implies the intercalation *** emits phosphorescence at 619 nm in the presence of thallium（I） *** lifetime of Ber increased from 38.4 ms to 43.4 ms with the increase of *** increase of lifetime is possibly attributed to the increase of rigidity of Ber after its intercalating into the ctDNA base *** explanation is also augmented by the anionic quenching *** behaves as a typical intercalating agent into *** intrinsic binding constant between Ber and ctDNA is（1.84±0.12）×10^4 L/mol and n=0.16.