检索结果-南通市图书馆

Controlling calcium and phosphate ion release of 3D printed bioactive ceramic scaffolds: An in vitro study

Journal of Advanced Ceramics 2017年第2期6卷 157-164页

作者： lukasz witek Yang SHI James SMAY Department of Biomaterials and Biomimetics College of Dentistry New York University New York NY USA School of Chemical Engineering Oklahoma State University Stillwater OK USA School of Materials Science and Engineering Oklahoma State University Tulsa OK USA

This paper characterizes in an in vitro setting the release of calcium (Ca) and phosphate (PO4) of 3D printed bioactive ceramic scaffold prepared from extrudable paste containing hydroxyapatite and β-tricalcium phosphate (β-TCP).Hydroxyapatite and β-TCP were calcined at 800℃ for 11 h,fabricated into four experimental groups (100％ HA,100％ β-TCP,15％/85％ HA/β-TCP,and 15％/85％ HA/β-TCP (design)),sintered to 1100 ℃ for 4 *** and phosphorus concentrations were evaluated using ICP spectroscopy,and the release of Ca and PO4 ions during dissolution of the CaP-based scaffolds was measured by submerging in 0.05 mol/L Tris(hydroxymethyl)aminomethane-HC1 and maintaining a temperature of 37 ℃.The Ca and PO4 concentrations of the solutions were measured with the utilization of a calcium assay kit and a phosphate assay kit and read in a UV-visible *** 100％ HA scaffold group showed the greatest concentration of Ca ions (～1.9 mg/dL),but ultimately released at a lower amount as time increased;the 100％ HA scaffold also showed the lowest total amount of calcium ions released over the course of *** results for the 100％ β-TCP were on the opposite of the HA with the highest amount of calcium ion release over the *** the PO4 ion release showed a similar trend as those observed with Ca ions with an apparent difference in the 100％ HA scaffold *** was nearly 0 mg/dL of the phosphate ions released in the first 24 h,in comparison to the amount of Ca ions released during the same time *** various formulations can lead to different properties of these bioactive ceramic scaffolds,it is important to understand how the tailoring of this important biphasic material can impact the long-term outcome of an ever-important in vivo clinical trial in the future.

关键词： 3D printing β-TCP scaffolds in vitro calcium phoshpate

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Sintering effects on chemical and physical properties of bioactive ceramics

引用

Journal of Advanced Ceramics 2013年第3期2卷 274-284页

作者： lukasz witek James SMAY Nelson R.F.A.SILVA Teja GUDA Joo L.ONG Paulo G.COELHO School of Chemical Engineering Oklahoma State University423 Engineering NorthStillwaterOKUSA Department of Biomaterials and Biomimetics New York University345 East 24th Street804S New YorkNYUSA Department of Operative Dentistry Universidade Federal de Minas GeraisPampulhaBelo HorizonteMGBrazil Department of Biomedical Engineering University of Texas San AntonioOne UTSA CircleSan AntonioTXUSA Department of Periodontology and Implant Dentistry College of DentistryNew York University345 East 24th StreetNew YorkNYUSA

The objective of this study was to characterize the chemical and physical properties of bioactive ceramics prepared from an aqueous paste containing hydroxyapatite(HA)and beta tri-calcium phosphate(β-TCP).Prior to formulating the paste,HA andβ-TCP were calcined at 800℃and 975℃(11 h),milled,and blended into 15%/85%HA/β-TCP volume-mixed *** cylindrical rods were subsequently sintered to 900℃,1100℃or 1250℃.The sintered specimens were characterized by helium pycnometry,X-ray diffraction(XRD),Fourier transform-infrared(FT-IR),and inductively coupled plasma(ICP)spectroscopy for evaluation of porosity,crystalline phase,functional-groups,and Ca:P ratio,*** properties were assessed via 3-point bending and diametral *** microstructural evaluation using scanning electron microscopy(SEM)showed larger pores and a broader pore size distribution(PSD)for materials sintered at 900℃and 1100℃,whereas the 1250℃samples showed more uniform *** quantification showed significantly higher porosity for materials sintered to 900℃and 1250℃(p<0.05).XRD indicated substantial deviations from the 15%/85%HA/β-TCP formulation following sintering where lower amounts of HA were observed when sintering temperature was *** testing demonstrated significant differences between calcination temperatures and different sintering regimes(p<0.05).Variation in chemical composition and mechanical properties of bioactive ceramics were direct consequences of calcination and sintering.

关键词： bone graft material chemical/physical characterization sintering bi-phasic calcium phosphate(BCP)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Loss of Notch signaling in skeletal stem cells enhances bone formation with aging

引用

Bone Research 2023年第4期11卷 737-750页

作者： Lindsey H.Remark Kevin Leclerc Malissa Ramsukh Ziyan Lin Sooyeon Lee Backialakshmi Dharmalingam Lauren Gillinov Vasudev V.Nayak Paulo El Parente Margaux Sambon Pablo J.Atria Mohamed A.E.Ali lukasz witek Alesha B.Castillo Christopher Y.Park Ralf H.Adams Aristotelis Tsirigos Sophie M.Morgani Philipp Leucht Department of Orthopaedic Surgery NYU Robert I.Grossman School of MedicineNew YorkNYUSA Applied Bioinformatics Laboratories NYU Grossman School of MedicineNew YorkNYUSA Institute of Comparative Molecular Endocrinology Ulm UniversityUlmGermany Max Planck Institute for Molecular Biomedicine Department of Tissue Morphogenesisand University of MünsterFaculty of MedicineD-48149 MünsterGermany Department of Biochemistry and Molecular Biology University of Miami Miller School of MedicineMiamiFLUSA Department of Pathology NYU Robert I.Grossman School of MedicineNew YorkNYUSA Biomaterials Division New York University College of DentistryNew YorkNYUSA Hansjörg Wyss Department of Plastic Surgery NYU Grossman School of MedicineNew York UniversityNew YorkNYUSA Department of Biomedical Engineering Tandon School of EngineeringNew York UniversityBrooklynNYUSA Department of Cell Biology NYU Robert I.Grossman School of MedicineNew YorkNYUSA

Skeletal stem and progenitor cells(SSPCs) perform bone maintenance and repair. With age, they produce fewer osteoblasts and more adipocytes leading to a loss of skeletal integrity. The molecular mechanisms that underlie this detrimental transformation are largely unknown. Single-cell RNA sequencing revealed that Notch signaling becomes elevated in SSPCs during aging. To examine the role of increased Notch activity, we deleted Nicastrin, an essential Notch pathway component, in SSPCs in vivo. Middle-aged conditional knockout mice displayed elevated SSPC osteo-lineage gene expression, increased trabecular bone mass, reduced bone marrow adiposity, and enhanced bone repair. Thus, Notch regulates SSPC cell fate decisions, and moderating Notch signaling ameliorates the skeletal aging phenotype, increasing bone mass even beyond that of young mice. Finally, we identified the transcription factor Ebf3 as a downstream mediator of Notch signaling in SSPCs that is dysregulated with aging, highlighting it as a promising therapeutic target to rejuvenate the aged skeleton.

关键词： Notch skeletal elevated

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Flutamide-induced alterations in transcriptional profiling of neonatal porcine ovaries

引用

Journal of Animal Science and Biotechnology 2019年第3期10卷 618-632页

作者： Katarzyna Knapczyk-Stwora Anna Nynca Renata E.Ciereszko lukasz Paukszto Jan P.Jastrzebski Elzbieta Czaja Patrycja witek Marek Koziorowski Maria Slomczynska Department of Endocrinology Institute of Zoology and Biomedical ResearchJagiellonian University in KrakowGronostajowa 9 Street30-387 KrakowPoland Laboratory of Molecular Diagnostics Faculty of Biology and BiotechnologyUniversity of Warmia and Mazury in OlsztynOlsztynPoland Department of Animal Anatomy and Physiology Faculty of Biology and BiotechnologyUniversity of Warmia and Mazury in OlsztynOlsztynPoland Department of Plant Physiology Genetics and BiotechnologyFaculty of Biology and BiotechnologyUniversity of Warmia and Mazury in OlsztynOlsztynPoland Department of Physiology and Reproduction of Animals University of RzeszowRzeszowPoland

Background: Androgens are involved in the regulation of ovarian development during fetal/neonatal *** chemicals displaying anti-androgenic activities may affect multiple signal transduction pathways by blocking endogenous androgen *** aim of the current study was to examine effects of the anti-androgen flutamide on the expression of coding transcripts and long non-coding RNAs(lncRNAs) in neonatal porcine *** employing RNA-Seq technology we aimed to extend our understanding of the role of androgens in neonatal folliculogenesis and examine the impact of the anti-androgen flutamide on ovarian ***: Piglets were subcutaneously injected with flutamide(50 mg/kg BW) or corn oil(controls) between postnatal days 1 and 10(n = 3/group).Ovaries were excised from the 11-day-old piglets and total cellular RNAs were isolated and ***: Flutamide-treated piglet ovaries showed 280 differentially expressed genes(DEGs;P-adjusted < 0.05 and log2 fold change ≥1.0) and 98 differentially expressed lncRNAs(DELs;P-adjusted < 0.05 and log2 FC ≥ 1.0).The DEGs were assigned to GO term,covering biological processes,molecular functions and cellular components,which linked the DEGs to functions associated with cellular transport,cell divisions and *** addition,STRING software demonstrated strongest interactions between genes related to cell *** between DEGs and DELs were also found,revealing that a majority of the genes targeted by the flutamide-affected lncRNAs were associated with intracellular transport and cell ***: Our results suggest that neonatal exposure of pigs to flutamide alters the expression of genes involved in ovarian cell proliferation,ovarian steroidogenesis and oocyte fertilization,which in turn may affect female reproduction in adult life.

关键词： Flutamide LncRNA Ovary Pig RNA-Seq Transcriptome

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A potato late blight resistance gene protects against multiple Phytophthora species by recognizing a broadly conserved RXLR-WY effector

引用

Molecular Plant 2022年第9期15卷 1457-1469页

作者： Xiao Lin Andrea Olave-Achury Robert Heal Marina Pais Kamil witek Hee-Kyung Ahn He Zhao Shivani Bhanvadia Hari S.Karki Tianqiao Song Chih-hang Wu Hiroaki Adachi Sophien Kamoun Vivianne G.A.A.Vleeshouwers Jonathan D.G.Jones The Sainsbury Laboratory University of East AngliaNorwich Research ParkNR47UH NorwichUK Wageningen UR Plant Breeding Wageningen University and ResearchDroevendaalsesteeg 16708 PB WageningenThe Netherlands Present address:Institute of Plant Protection Jiangsu Academy of Agricultural SciencesNanjing 210014P.R.China Present address:Institute of Plant and Microbial Biology Academia SinicaTaipei 11529 Taiwan Present address:Laboratory of Crop Evolution Graduate School of AgricultureKyoto UniversityMozumeMukoKyoto 617-0001Japan Present address:Lipman Family Farms 21106 Six Ls Farm RdEsteroFloridaUnited States

Species of the genus Phytophthora,the plant killer,cause disease and reduce yields in many crop *** many Resistance to Phytophthora infestans(Rpi)genes effective against potato late blight have been cloned,few have been cloned against other Phytophthora *** Rpi genes encode nucleotide-binding domain,leucine-rich repeat-containing(NLR)immune receptor proteins that recognize RXLR(Arg-X-Leu-Arg)***,whether NLR proteins can recognize RXLR effectors from multiple Phytophthora species has rarely been ***,we identified a new RXLR-WY effector AVRamr3 from *** that is recognized by Rpi-amr3 from a wild Solanaceae species Solanum ***-amr3 associates with AVRamr3 in ***3 is broadly conserved in many different Phytophthora species,and the recognition of AVRamr3 homologs by Rpi-amr3 activates resistance against multiple Phytophthora pathogens,including the tobacco black shank disease and cacao black pod disease pathogens *** and ***-amr3 is thus the first characterized resistance gene that acts against *** or *** findings suggest a novel path to redeploy known R genes against different important plant pathogens.

关键词： Rpi-amr3 AVRamr3 potato late blight Phytophthora disease RXLR-WY effector Solanum americanum

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Dose Reduction to the Scalp with Hippocampal Sparing Is Achievable with Intensity Modulated Radiotherapy

引用

International Journal of Medical Physics, Clinical Engineering and Radiation Oncology 2014年第3期3卷 176-182页

作者： Matthew witek Yelena Vahknenko Joshua Siglin Amy Harrison Ying Xiao Haison Lui David Andrews Wenyin Shi Department of Neurosurgery Thomas Jefferson University Hospital Philadelphia USA Department of Radiation Oncology Thomas Jefferson University Hospital Philadelphia USA

We evaluated the feasibility of combined hippocampal- and scalp-sparing intensity-modulated radiotherapy (IMRT) plans. This study included 7 patients who received conventional palliative whole brain radiation treatment (WBRT) for brain metastasis. The brain, hippocampus, and scalp were contoured and replanned with intensity modulated radiation therapy. The prescription dose was 30 Gray (Gy) in 10 fractions with hippocampus and normal structure constraints per the Radiation Therapy Oncology Group (RTOG) 0933 protocol. Further planning was done to minimize the scalp dose while maintaining the dose constraints for the hippocampus. Dose volume histograms were obtained from conventional opposed lateral fields, IMRT and compared. Planning target volume (PTV) coverage for all plans fell within the RTOG 0933 critical structure acceptable variation category. When compared to traditional opposed lateral fields, the IMRT plan with combined hippocampal- and scalp-sparing constraints was able to significantly reduce the max and mean scalp dose as well as the percentage of scalp receiving 10 and 20 Gy by 46% and 35%, respectively, while maintaining acceptable RTOG 0933 hippocampal dose variations. We conclude that acceptable PTV coverage and sparing of the scalp and hippocampus can be accomplished using a 9-field non-coplanar IMRT plan. Prospective study is warranted to understand the impact on radiation-induced alopecia.

关键词： Hippocampal Scalp Sparing WBRT IMRT

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Profiles of interferon-gamma and interleukin-2 in patients after allogeneic hematopoietic stem cell transplantation

引用

World Journal of Biological Chemistry 2022年第4期13卷 72-82页

作者： Malwina Rybicka-Ramos Mirosław Markiewicz Aleksandra Suszka-Świtek Ryszard Wiaderkiewicz Sylwia Mizia Monika Dzierżak-Mietła Krzysztof Białas Department of Hematology Specialist Hospital No.1 in BytomBytom 41-902Poland Department of Hematology University of RzeszowCollege of Medical SciencesInstitute of Medical SciencesRzeszów 35-315Poland Department of Histology and Embryology School of Medicine in KatowiceMedical University of SilesiaKatowice 40-752Poland Department of Population Health Division of Epidemiology and Health EducationWroclaw Medical UniversityWrocław 51-618Poland Department of Bone Marrow Transplantation and Hematooncology Maria Sklodowska-Curie National Research Institute of OncologyGliwice 44-102Poland Department of Hematology and Bone Marrow Transplantation SPSK-M HospitalKatowice 40-027Poland

BACKGROUND Allogeneic hematopoietic stem cell transplantation(allo-HSCT)may be related to the occurrence of complications,including graft-versus-host disease(GvHD)and *** pathogenesis of acute GvHD is connected with T lymphocytes,which identify alloantigens on host's antigen-presenting cells,activate production of interferon-gamma(IFN-gamma)and interleukin-2(IL-2),and act on the immune effector cells and damage tissues and *** The aim of the study was to investigate and distinguish serum concentration profiles of IFN-gamma and IL-2 within a 30-d period after *** We enrolled 62 patients,i.e.,30(48%)male and 32(52%)female subjects[median age 49.5(19-68)years],after allo-HSCT from siblings(n=12)or unrelated donors(n=50)due to acute myeloid leukemia with myeloablative conditioning(n=26;42%)and with non-myeloablative conditioning(n=36;58%).All patients were given standard immunosuppressive therapy with cyclosporin-A and methotrexate and pre-transplant antithymocyte globulin in the unrelated *** samples were collected pre-transplant before and after(on day-1)the conditioning therapy and on days+2,+4,+6,+10,+20,and+30 after *** levels of IL-2 and IFNgamma were determined using *** Patients were divided into four groups depending on the presence of acute GvHD and clinical manifestations of *** I included patients with neither acute GvHD nor infections[n=15(24%)],group II consisted of patients with infections without acute GvHD[n=17(27%)],group III was comprised of patients with acute GvHD without infections[n=9(15%)],and group IV included patients with both acute GvHD and infections[n=21(34%)].IFN-gamma concentrations were higher in Group II than in other groups on days+20(P=0.014)and+30(P=0.008).Post-hoc tests showed lower concentrations of IFN-gamma on day+30 in groups I(P=0.039)and IV(P=0.017)compared to group *** levels of IL-2 were mostly *** Serum levels of IFN-gamma fol

关键词： Interleukin-2 Interferon-gamma Cytokine profiles Acute myeloid leukemia Allogeneic hematopoietic stem cell transplantation Acute graft-versus-host disease

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pm21 from Haynaldia villosa Encodes a CC-NBS- LRR Protein Conferring Powdery Mildew Resistance in Wheat

引用

Molecular Plant 2018年第6期11卷 874-878页

作者： Liping Xing Ping Hu Jiaqian Liu Kamil witek Shuang Zhou Jiefei Xu Weihao Zhou Li Gao Zhenpu Huang Ruiqi Zhang Xiue Wang Peidu Chen Haiyan Wang Jonathan D.G. Jones Miroslava Karafiatova Jan Vrana Jan Baros Jaroslav Dolezel Yuanchun Tian Yufeng Wu Aizhong Cao National Key Laboratory of Crop Genetics and Germplasm Enhancement Cytogenetics Institute Nanjing Agricultural University/JGIC-MCP Nanjing 210095 China The Sainsbury Laboratory Norwich Research Park Norwich NR4 7UH UK Institute of Experimental Botany Centre of the Region Hana for Biotechnological and Agricultural Research Slechtitelu 31 78371 OlomoucCzech Republic

Dear Editor Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici （Bgt）, is a destructive disease of wheat throughout the world. One of the most important environmental-friendly and economical methods to reduce wheat loss caused by Bgt is to develop highly resistant varieties （Kuraparthy et al., 2007）. Pm21 from the wild species Haynaldia villosa （also known as Dasypyrum villosum） confers high resistance to Bgt in wheat throughout all growth stages. It has now become one of the most highly effective genetic loci introgressed into wheat from wild species, and the commercial varieties harboring Pm21 have been widely used in wheat production with more than 4 million hectares in China.

关键词： Pm21 小麦粉蛋白质编码环境友好生长阶段小麦生产还原剂

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Flutamide-induced alterations in transcriptional profiling of neonatal porcine ovaries.

引用

畜牧与生物技术杂志(英文版) 2019年第3期

作者： Katarzyna Knapczyk-Stwora Anna Nynca Renata E.Ciereszko lukasz Paukszto Jan P.Jastrzebski Elzbieta Czaja Patrycja witek Marek Koziorowski Maria Slomczynska

来源：评论

学校读者我要写书评

暂无评论

向日常生活致敬:2018 danishdesign MAKERS家具展

引用

现代装饰 2018年第12期 86-93页

作者： Alvilde Holm Antonio Scaffidi Edvard & Steenfatt Ellinor Ericsson Evalou Hauge Gustav Max Lindholm Jesper Junge Jonas Lyndby Jensen Nordvink/Anne N?rbjerg & Sanne Kyed ?rn Duvald Petter Th?rne Rasmus Warberg Rikke Frost Sahtel Mouritsen Lystager Signe?.Lund & Isabel Ahm Thomas Albertsen Thomas e.Alken Troels Flensted witek Golik danishdesign MAKERS Kristine Funch & Emilia Therese 彭佳 Studio Fem-Anders Engholm & Sarah Cramer

本届2018danishdesign MAKERS家具展的主题为“向日常生活致敬”,其灵感来源于丹麦诗人Dan Turell诗歌中关于日常生活之美的语句,同时也表达了对于如今设计行业中奢侈浪费现象的思考。

关键词：家具展浪费现象设计行业 Dan

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：