Profiles of interferon-gamma and interleukin-2 in patients after allogeneic hematopoietic stem cell transplantation

作     者：Malwina Rybicka-Ramos Mirosław Markiewicz Aleksandra Suszka-Świtek Ryszard Wiaderkiewicz Sylwia Mizia Monika Dzierżak-Mietła Krzysztof Białas 

作者机构：Department of HematologySpecialist Hospital No.1 in BytomBytom 41-902Poland Department of HematologyUniversity of RzeszowCollege of Medical SciencesInstitute of Medical SciencesRzeszów 35-315Poland Department of Histology and EmbryologySchool of Medicine in KatowiceMedical University of SilesiaKatowice 40-752Poland Department of Population HealthDivision of Epidemiology and Health EducationWroclaw Medical UniversityWrocław 51-618Poland Department of Bone Marrow Transplantation and HematooncologyMaria Sklodowska-Curie National Research Institute of OncologyGliwice 44-102Poland Department of Hematology and Bone Marrow TransplantationSPSK-M HospitalKatowice 40-027Poland 

出 版 物：《World Journal of Biological Chemistry》 (世界生物化学杂志（英文版）（电子版）)

年 卷 期：2022年第13卷第4期

页      面：72-82页

学科分类：1002[医学-临床医学] 1010[医学-医学技术（可授医学、理学学位）] 100215[医学-康复医学与理疗学] 10[医学] 

主　　题：Interleukin-2 Interferon-gamma Cytokine profiles Acute myeloid leukemia Allogeneic hematopoietic stem cell transplantation Acute graft-versus-host disease 

摘      要：BACKGROUND Allogeneic hematopoietic stem cell transplantation(allo-HSCT)may be related to the occurrence of complications,including graft-versus-host disease(GvHD)and *** pathogenesis of acute GvHD is connected with T lymphocytes,which identify alloantigens on host s antigen-presenting cells,activate production of interferon-gamma(IFN-gamma)and interleukin-2(IL-2),and act on the immune effector cells and damage tissues and *** The aim of the study was to investigate and distinguish serum concentration profiles of IFN-gamma and IL-2 within a 30-d period after *** We enrolled 62 patients,i.e.,30(48%)male and 32(52%)female subjects[median age 49.5(19-68)years],after allo-HSCT from siblings(n=12)or unrelated donors(n=50)due to acute myeloid leukemia with myeloablative conditioning(n=26;42%)and with non-myeloablative conditioning(n=36;58%).All patients were given standard immunosuppressive therapy with cyclosporin-A and methotrexate and pre-transplant antithymocyte globulin in the unrelated *** samples were collected pre-transplant before and after(on day-1)the conditioning therapy and on days+2,+4,+6,+10,+20,and+30 after *** levels of IL-2 and IFNgamma were determined using *** Patients were divided into four groups depending on the presence of acute GvHD and clinical manifestations of *** I included patients with neither acute GvHD nor infections[n=15(24%)],group II consisted of patients with infections without acute GvHD[n=17(27%)],group III was comprised of patients with acute GvHD without infections[n=9(15%)],and group IV included patients with both acute GvHD and infections[n=21(34%)].IFN-gamma concentrations were higher in Group II than in other groups on days+20(P=0.014)and+30(P=0.008).Post-hoc tests showed lower concentrations of IFN-gamma on day+30 in groups I(P=0.039)and IV(P=0.017)compared to group *** levels of IL-2 were mostly *** Serum levels of IFN-gamma fol