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The LpHsfA2-molecular module confers thermotolerance via fine tuning of its transcription in perennial ryegrass(Lolium perenne L.)

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Journal of Integrative Plant Biology 2024年第11期66卷 2346-2361页

作者： Guangjing Ma Zhihao Liu Shurui Song Jing Gao Shujie Liao Shilong cao Yan Xie liwen cao Longxing Hu Haichun Jing Liang Chen CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical GardenChinese Academy of SciencesWuhan 430074China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Edible Wild Plants Conservation and Utilization Hubei Normal UniversityHuangshi 435002China Department of Pratacultural Sciences College of AgronomyHunan Agricultural UniversityChangsha 410128China Key Laboratory of Plant Resources Institute of BotanyChinese Academy of SciencesBeijing 100093China Academician Workstation of Agricultural High-tech Industrial Area of the Yellow River Delta National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali LandDongying 257300China

Temperature sensitivity and tolerance play a key role in plant survival and *** ryegrass(Lolium perenne L.),widely cultivated in cool-season for forage supply and turfgrass,is extremely susceptible to high temperatures,therefore serving as an excellent grass for dissecting the genomic and genetic basis of high-temperature *** this study,expression analysis revealed that LpHsfA2,an important gene associated with high-temperature tolerance in perennial ryegrass,is rapidly and substantially induced under heat ***,heat-tolerant varieties consistently display elevated expression levels of LpHsfA2 compared with heat-sensitive *** haplotype analysis of the LpHsfA2 promoter indicated an uneven distribution of two haplotypes(HsfA2^(Hap1) and HsfA2^(Hap2)) across varieties with differing heat ***,the HsfA2^(Hap1) allele is predominantly present in heat-tolerant varieties,while the HsfA2^(Hap2) allele exhibits the opposite *** of LpHsfA2 confers enhanced thermotolerance,whereas silencing of LpHsfA2 compromises heat ***,LpHsfA2 orchestrates its protective effects by directly binding to the promoters of LpHSP18.2 and LpAPX1 to activate their expression,preventing the non-specific misfolding of intracellular protein and the accumulation of reactive oxygen species in ***,LpHsf A4 and LpHsf A5 were shown to engage directly with the promoter of LpHsfA2,upregulating its expression as well as the expression of LpHSP18.2 and LpAPX1,thus contributing to enhanced heat ***,LpHsfA2 possesses autoregulatory ability by directly binding to its own promoter to modulate the *** on these findings,we propose a model for modulating the thermotolerance of perennial ryegrass by precisely regulating the expression of ***,these findings provide a scientific basis for the development of thermotolerant perennial ryegrass culti

关键词： haplotype heat shock transcription factors heat tolerance perennial ryegrass

Simultaneous gene editing of three homoeoalleles in self-incompatible allohexaploid grasses

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Journal of Integrative Plant Biology 2021年第8期63卷 1410-1415页

作者： Liang Zhang Tao Wang Guangyang Wang Aoyue Bi Misganaw Wassie Yan Xie liwen cao Huawei Xu Jinmin Fu Liang Chen Yang Zhao Tao Hu CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical GardenThe Innovative Academy of Seed DesignThe Chinese Academy of SciencesWuhan430074 China Center of Economic Botany Core Botanical GardensThe Chinese Academy of SciencesWuhan430074 China University of Chinese Academy of Sciences Beijing100049 China Shanghai Center for Plant Stress Biology and CAS Center of Excellence in Molecular Plant SciencesThe Chinese Academy of SciencesShanghai200032 China College of Agriculture Henan University of Science and TechnologyLuoyang471023 China School of Resources and Environmental Engineering Ludong UniversityYantai264011 China

Clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)system has been widely used for precise gene editing in ***,simultaneous gene editing of multiple homoeoalleles remains challenging,especially in self-incompatible polyploid ***,we simultaneously introduced targeted mutations in all three homoeoalleles of two genes in the self-incompatible allohexaploid tall fescue,using both CRISPR/Cas9 and LbCas12a(LbCpf1)***-of-function mutants of FaPDS exhibited albino leaves,while knockout of FaHSP17.9 resulted in impaired heat resistance in T0 generation of tall ***,these mutations were *** findings demonstrate the feasibility of generating loss-of-function mutants in T0 generation polyploid perennial grasses using CRISPR/Cas systems.

关键词： allohexaploid Cas12a(Cpf1) CRISPR/Cas9 self-incompatible tall fescue

EEG-Based Emotion Recognition Using Dynamic Spatial-Spectral-Temporal Network

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EEG-Based Emotion Recognition Using Dynamic Spatial-Spectral...

第43届中国控制会议

作者： liwen cao Zichen Luo Biao Sun School of Electrical and Information Engineering Tianjin University

EEG-based emotion recognition has demonstrated significant potential in various domains,for example,emotion detection of patients with disorders of consciousness,cognitive load assessment,emotion detection of drivers and so *** the realm of brain-computer interface research and development,the ability to accurately and effectively recognize emotions is of significant *** this paper,we propose a multi-channel EEG emotion recognition method called dynamic spatialspectral-temporal network(DSSTNet).It comprises three components:(1) Spatial features extractor consists of a graph converter and a graph convolutional *** first converts the EEG signal into graph-structured ***,the graph convolutional network is used to dynamically optimize the adjacency matrix to obtain the spatial features between the channels.(2) Band attention module composed of semi-global pooling,local cross-band interaction,and adaptive weighting for further extracting spectral information.(3) Temporal features extractor that extracts deep temporal information by stacking multiple one-dimensional convolution *** results of comparative experiments show that the classification accuracy of the proposed method is better than that of a series of comparison methods,and the robustness is relatively *** results of ablation experiments validate the effectiveness of each module proposed in the method.

关键词： EEG classification adjacency matrix graph convolutional network(GCN) subject-dependent deep learning

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优选泥浆配方的模糊数学方法

煤田地质与勘探 2000年第5期28卷 63-64页

作者：曹丽文中国矿业大学资环学院徐州221008

泥浆试验属于多因素多指标评价试验。应用模糊数学理论中的多目标模糊决策法 ,根据不同地层的钻进需要 ,借助计算机编程计算优选泥浆配方是一个行之有效的方法。

关键词：泥浆多目标决策优选设计模糊数学计算机

SGDE-Net:SAM-Guided Dual-Path Encoding NetWork for Medical Image Segmentation

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SGDE-Net:SAM-Guided Dual-Path Encoding NetWork for Medical I...

第43届中国控制会议

作者： Zichen Luo Xinshan Zhu Di He liwen cao Biao Sun School of Electrical and Information Engineering Tianjin University

Accurate segmentation is crucial in medical image diagnosis and treatment planning. Currently, segmentation methods in medical images primarily utilize Transformer and Convolutional Neural Networks(CNN) architectures for feature *** former leverages its modeling capability for long-range dependencies to capture global information, while the latter is more adept at learning local information. Previous studies have sought to absorb their respective advantages by directly combining Transformer and CNN, potentially resulting in feature inconsistency during the learning process. To solve this problem, we propose a novel SAM-guided dual-path encoding network, abbreviated as SGDE-Net. Specifically, we design a multi-scale cascade encoder, which is a multi-scale CNN encoder guided by a Segment Anything Model(SAM) encoder, namely SAM guided encoder(SGE), to combine global dependency information with local information. Meanwhile, SGE could successfully apply the foundation model encoder with strong feature extraction capabilities to downstream medical segmentation tasks as ***, we devise a Hybrid Fusion Mechanism(HFM) including feature fusion methods tailored to the requirements of different stages, thereby reducing redundant information. Our experiments show that the SGE encoder significantly outperforms CNN and Transformer-based methods, achieving state-of-the-art DICE and mIoU scores.

关键词： Medical Image Segmentation Deep Learning CNN Transformer Feature Fusion

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LOCA事故下碳化硅复合包壳失效概率计算

核技术 2023年第9期46卷 111-116页

作者：曹力文易柏全郝祖龙华北电力大学核科学与工程学院北京102206

碳化硅(SiC)复合包壳是未来轻水堆耐事故燃料(Accident Tolerant Fuel,ATF)包壳候选方案之一。本文主要研究了在失水事故(Loss Of Coolant Accident,LOCA)下双层SiC结构包壳的失效概率问题。基于SiC复合材料包壳失效概率计算方法,采用... 详细信息

碳化硅(SiC)复合包壳是未来轻水堆耐事故燃料(Accident Tolerant Fuel,ATF)包壳候选方案之一。本文主要研究了在失水事故(Loss Of Coolant Accident,LOCA)下双层SiC结构包壳的失效概率问题。基于SiC复合材料包壳失效概率计算方法,采用准稳态方法模拟计算了瞬态工况下的SiC复合材料包壳失效概率。通过分析各种应力在事故工况下的占比,对Weibull分布的两个特征参数进行敏感性分析;研究了不同燃耗对于失效概率的影响,模拟了在不同层厚比下包壳的失效概率。结果表明:复合层所占比例的变化、Weibull参数改变、在不同燃耗下发生LOCA事故,对于SiC复合材料包壳的瞬态失效概率都有着较为明显的影响。本文的研究有助于耐事故燃料包壳的开发与设计,为SiC复合材料包壳失效概率的进一步研究提供参考。

关键词：压水堆碳化硅复合包壳失水事故失效概率

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金刚石串珠绳的磨削与磨损机理

工具技术 2007年第9期41卷 29-33页

作者：曹立文黑龙江大学

介绍了金刚石串珠绳的结构和类型。结合海底石油管道切割试验研究了金刚石串珠绳的磨削机理,分析了金刚石串珠绳微观磨损形态和特性;通过对磨削后的串珠表面形貌显微照片的观察,验证了分析结果的正确性。针对海底油气管道切割定量分析... 详细信息

介绍了金刚石串珠绳的结构和类型。结合海底石油管道切割试验研究了金刚石串珠绳的磨削机理,分析了金刚石串珠绳微观磨损形态和特性;通过对磨削后的串珠表面形貌显微照片的观察,验证了分析结果的正确性。针对海底油气管道切割定量分析了串珠的磨损过程和规律,得到了串珠绳的耐磨损率与进给速度、串珠绳的耐磨损率与切削速度的关系曲线。分析了串珠绳磨削加工表面质量。

关键词：金刚石绳锯机磨削机理耐磨损率表面质量

两种应力路径下非饱和污染土强度与微观结构研究

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应用基础与工程科学学报 2019年第3期37卷 602-611页

作者：郭玉良曹丽文霍攀中国矿业大学资源与地球科学学院江苏徐州221116 中电工程东北院公司吉林长春130021

以非饱和CH3COOH污染黏土为研究对象,完成不同基质吸力下加压和减压两种应力路径的三轴试验,得到了强度参数和强度公式,并利用扫描电镜(SEM)观测污染黏土微观结构,分析污染黏土微观结构的变化特征.发现非饱和土的基质吸力可增大土体内... 详细信息

以非饱和CH3COOH污染黏土为研究对象,完成不同基质吸力下加压和减压两种应力路径的三轴试验,得到了强度参数和强度公式,并利用扫描电镜(SEM)观测污染黏土微观结构,分析污染黏土微观结构的变化特征.发现非饱和土的基质吸力可增大土体内聚力和内摩擦角;常规三轴试验与减压三轴试验的应力-应变曲线呈现出不同的强度变化规律;基质吸力由小变大,黏土颗粒分布的均匀性变差,孔隙尺寸从大孔向中孔再向小孔逐渐过渡,说明基质吸力可促进土体固结;基质吸力的增加对减压路径下土体抗剪强度的提高效果优于对加压路径下的影响.

关键词：非饱和污染土应力路径强度微观结构

深部煤层CO_(2)注入煤岩力学响应特征及机理研究进展

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地球科学 2022年第5期47卷 1849-1864页

作者：桑树勋牛庆合曹丽文王伟中国矿业大学碳中和研究院江苏省煤基温室气体减排与资源化利用重点实验室江苏徐州221116 石家庄铁道大学省部共建交通工程结构力学行为与系统安全国家重点实验室河北石家庄050043 河北省金属矿山安全高效开采技术创新中心河北石家庄050043 中国矿业大学资源与地球科学学院江苏徐州221008

为揭示CO_(2)注入煤岩力学响应特征及机理,回顾了CO_(2)注入煤岩力学性质影响因素、CO_(2)注入对煤岩大分子-孔隙-裂隙结构改造作用和煤岩力学参数的统计模型、理论模型与智能预测模型.结果表明:CO_(2)注入煤岩力学性质受控于煤阶、CO_... 详细信息

为揭示CO_(2)注入煤岩力学响应特征及机理,回顾了CO_(2)注入煤岩力学性质影响因素、CO_(2)注入对煤岩大分子-孔隙-裂隙结构改造作用和煤岩力学参数的统计模型、理论模型与智能预测模型.结果表明:CO_(2)注入煤岩力学性质受控于煤阶、CO_(2)压力、水分、围压和时间等因素,CO_(2)注入压力的增高、水的加入及时间的延长均会进一步降低煤岩力学性质,而围压对CO_(2)注入力学性能弱化具有一定改善作用.CO_(2)水溶液通过溶胀作用、萃取作用和塑化作用促使煤岩大分子结构重组,通过微晶结构改变、化学溶蚀和非均匀变形改造煤岩孔隙结构,通过化学溶蚀、膨胀应力和化学-应力耦合作用诱发煤岩结构损伤,均不同程度引起煤岩力学性能弱化.在CO_(2)注入煤岩力学参数预测模型中,类Langmuir模型、广延指数模型和修正的粘聚力模型具有明确的物理意义,而智能预测模型具有更高的预测精度,预测准确度可达99%以上.本次研究为科学评价CO_(2)-ECBM安全性和促进深部煤层CO_(2)高效注入奠定了理论基础.

关键词： CO_(2)-ECBM 力学性质弱化机理多尺度结构预测模型油气地质