The LpHsfA2-molecular module confers thermotolerance via fine tuning of its transcription in perennial ryegrass(Lolium perenne L.)

作     者：Guangjing Ma Zhihao Liu Shurui Song Jing Gao Shujie Liao Shilong Cao Yan Xie Liwen Cao Longxing Hu Haichun Jing Liang Chen 

作者机构：CAS Key Laboratory of Plant Germplasm Enhancement and Specialty AgricultureWuhan Botanical GardenChinese Academy of SciencesWuhan 430074China University of Chinese Academy of SciencesBeijing 100049China Key Laboratory of Edible Wild Plants Conservation and UtilizationHubei Normal UniversityHuangshi 435002China Department of Pratacultural SciencesCollege of AgronomyHunan Agricultural UniversityChangsha 410128China Key Laboratory of Plant ResourcesInstitute of BotanyChinese Academy of SciencesBeijing 100093China Academician Workstation of Agricultural High-tech Industrial Area of the Yellow River DeltaNational Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali LandDongying 257300China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2024年第66卷第11期

页      面：2346-2361页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Key R&D Program of China(2022YFF1003200) the National Natural Science Foundation of China(NSFC)(Grant Nos.32001394,32102431 and 32101430) the Science&Technology Specific Projects in Agricultural High-tech Industrial Demonstration Area of the Yellow River Delta(Grant No.2022SZX13) the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDA26050201) the Major Science and Technology Innovation Project of Shandong Province(2022LZGC018) 

主　　题：haplotype heat shock transcription factors heat tolerance perennial ryegrass 

摘      要：Temperature sensitivity and tolerance play a key role in plant survival and *** ryegrass(Lolium perenne L.),widely cultivated in cool-season for forage supply and turfgrass,is extremely susceptible to high temperatures,therefore serving as an excellent grass for dissecting the genomic and genetic basis of high-temperature *** this study,expression analysis revealed that LpHsfA2,an important gene associated with high-temperature tolerance in perennial ryegrass,is rapidly and substantially induced under heat ***,heat-tolerant varieties consistently display elevated expression levels of LpHsfA2 compared with heat-sensitive *** haplotype analysis of the LpHsfA2 promoter indicated an uneven distribution of two haplotypes(HsfA2^(Hap1) and HsfA2^(Hap2)) across varieties with differing heat ***,the HsfA2^(Hap1) allele is predominantly present in heat-tolerant varieties,while the HsfA2^(Hap2) allele exhibits the opposite *** of LpHsfA2 confers enhanced thermotolerance,whereas silencing of LpHsfA2 compromises heat ***,LpHsfA2 orchestrates its protective effects by directly binding to the promoters of LpHSP18.2 and LpAPX1 to activate their expression,preventing the non-specific misfolding of intracellular protein and the accumulation of reactive oxygen species in ***,LpHsf A4 and LpHsf A5 were shown to engage directly with the promoter of LpHsfA2,upregulating its expression as well as the expression of LpHSP18.2 and LpAPX1,thus contributing to enhanced heat ***,LpHsfA2 possesses autoregulatory ability by directly binding to its own promoter to modulate the *** on these findings,we propose a model for modulating the thermotolerance of perennial ryegrass by precisely regulating the expression of ***,these findings provide a scientific basis for the development of thermotolerant perennial ryegrass culti