Simultaneous gene editing of three homoeoalleles in self-incompatible allohexaploid grasses

作     者：Liang Zhang Tao Wang Guangyang Wang Aoyue Bi Misganaw Wassie Yan Xie Liwen Cao Huawei Xu Jinmin Fu Liang Chen Yang Zhao Tao Hu Liang Zhang;Tao Wang;Guangyang Wang;Aoyue Bi;Misganaw Wassie;Yan Xie;Liwen Cao;Huawei Xu;Jinmin Fu;Liang Chen;Yang Zhao;Tao Hu

作者机构：CAS Key Laboratory of Plant Germplasm Enhancement and Specialty AgricultureWuhan Botanical GardenThe Innovative Academy of Seed DesignThe Chinese Academy of SciencesWuhan430074 China Center of Economic BotanyCore Botanical GardensThe Chinese Academy of SciencesWuhan430074 China University of Chinese Academy of SciencesBeijing100049 China Shanghai Center for Plant Stress Biologyand CAS Center of Excellence in Molecular Plant SciencesThe Chinese Academy of SciencesShanghai200032 China College of AgricultureHenan University of Science and TechnologyLuoyang471023 China School of Resources and Environmental EngineeringLudong UniversityYantai264011 China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2021年第63卷第8期

页      面：1410-1415页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：This work was supported by the National Natural Science Foundation of China(31772349,31672482,and 31401915) the Major Science and Technology Innovation Project of Shandong Province(2019JZZY010726) 

主　　题：allohexaploid Cas12a(Cpf1) CRISPR/Cas9 self-incompatible tall fescue 

摘      要：Clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)system has been widely used for precise gene editing in ***,simultaneous gene editing of multiple homoeoalleles remains challenging,especially in self-incompatible polyploid ***,we simultaneously introduced targeted mutations in all three homoeoalleles of two genes in the self-incompatible allohexaploid tall fescue,using both CRISPR/Cas9 and LbCas12a(LbCpf1)***-of-function mutants of FaPDS exhibited albino leaves,while knockout of FaHSP17.9 resulted in impaired heat resistance in T0 generation of tall ***,these mutations were *** findings demonstrate the feasibility of generating loss-of-function mutants in T0 generation polyploid perennial grasses using CRISPR/Cas systems.