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Gene-knockout by iSTOP enables rapid reproductive disease modeling and phenotyping in germ cells of the founder generation

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Science China(Life Sciences) 2024年第5期67卷 1035-1050页

作者： Yaling wang Jingwen Chen Xueying Huang Bangguo Wu Peng Dai Feng Zhang Jinsong Li lingbo wang State Key Laboratory of Genetic Engineering Shanghai Key Laboratory of Metabolic Remodeling and HealthInstitute of Metabolism and Integrative BiologyFudan UniversityShanghai 200438China Institute of Reproduction and Development Obstetrics and Gynecology HospitalFudan UniversityShanghai 200011China NHC Key Lab of Reproduction Regulation(Shanghai Institute for Biomedical and Pharmaceutical Technologies) School of PharmacyFudan UniversityShanghai 200433China Shanghai Key Laboratoryof Maternal and Fetal Medicine Clinical and Translational Research Center of Shanghai First Maternity and Infant HospitalFrontier Science Center for Stem Cell ResearchSchoolof Life Sciences and TechnologyTongji UniversityShanghai 200092China State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China

Cytosine base editing achieves C·G-to-T·A substitutions and can convert four codons(CAA/CAG/CGA/TGG)into STOP-codons(induction of STOP-codons,iSTOP)to knock out genes with reduced *** enables direct phenotyping in founders’somatic cells,but it remains unknown whether this works in founders’germ cells so as to rapidly reveal novel genes for ***,we initially establish that iSTOP in mouse zygotes enables functional characterization of known genes in founders’germ cells:Cfap43-iSTOP male founders manifest expected sperm features resembling human“multiple morphological abnormalities of the flagella”syndrome(i.e.,MMAF-like features),while oocytes of Zp3-iSTOP female founders have no zona *** further illustrate iSTOP’s utility for dissecting the functions of unknown genes with Ccdc183,observing MMAF-like features and male infertility in Ccdc183-iSTOP founders,phenotypes concordant with those of Ccdc183-KO *** ultimately establish that CCDC183 is essential for sperm morphogenesis through regulating the assembly of outer dynein arms and participating in the intra-flagellar *** study demonstrates iSTOP as an efficient tool for direct reproductive disease modeling and phenotyping in germ cells of the founder generation,and rapidly reveals the essentiality of Ccdc183 in fertility,thus providing a time-saving approach for validating genetic defects(like nonsense mutations)for human infertility.

关键词： disease modeling infertility multiple morphological abnormalities of the flagella(MMAF) induction of STOP-codons(iSTOP CRISPR-STOP) sperm motility

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Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice

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Journal of Genetics and Genomics 2024年第4期51卷 407-418页

作者： Yaling wang Xueying Huang Guoying Sun Jingwen Chen Bangguo Wu Jiahui Luo Shuyan Tang Peng Dai Feng Zhang Jinsong Li lingbo wang Shanghai Key Laboratory of Metabolic Remodeling and Health Institute of Metabolism and Integrative BiologyFudan UniversityShanghai 200438China Institute of Reproduction and Development Obstetrics and Gynecology HospitalFudan UniversityShanghai 200011China Shanghai Key Laboratory of Maternal and Fetal Medicine Clinical and Translational Research Center of Shanghai First Maternity and Infant HospitalFrontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China

During spermiogenesis,haploid spermatids undergo dramatic morphological changes to form slender sperm flagella and cap-like acrosomes,which are required for successful *** deformities in flagella cause a male infertility syndrome,multiple morphological abnormalities of the flagella(MMAF),while acrosomal hypoplasia in some cases leads to sub-optimal embryonic developmental ***,evidence regarding the occurrence of acrosomal hypoplasia in MMAF is ***,we report the generation of base-edited mice knocked out for coiled-coil domain-containing 38(Ccdc38)via inducing a nonsense mutation and find that the males are *** Ccdc38-KO sperm display acrosomal hypoplasia and typical MMAF *** find that the acrosomal membrane is loosely anchored to the nucleus and fibrous sheaths are disorganized in Ccdc38-KO *** analyses reveal that Ccdc38 knockout causes a decreased level of TEKT3,a protein associated with acrosome biogenesis,in testes and an aberrant distribution of TEKT3 in *** finally show that intracytoplasmic sperm injection overcomes Ccdc38-related *** study thus reveals a previously unknown role for CCDC38 in acrosome biogenesis and provides additional evidence for the occurrence of acrosomal hypoplasia in MMAF.

关键词： Acrosomal hypoplasia Multiple morphological abnormalities of the flagella(MMAF) Infertility Sperm motility Asthenoteratozoospermia Disease modeling

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临兴区块深部煤层气井生产特征及影响因素

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煤田地质与勘探 2024年第8期52卷 69-78页

作者：王志壮吴鹏孙强刘锐王令波中联煤层气有限责任公司北京100016

【目的】深部煤层气生产特征与中浅部煤层气生产特征具有明显区别,且同一区块内深部煤层气排采井也存在差异性。相关研究尚处于探索阶段,深入分析深部煤层气生产特征差异的影响因素对后续大规模开发阶段的产能预测与生产管理具有重要意... 详细信息

【目的】深部煤层气生产特征与中浅部煤层气生产特征具有明显区别,且同一区块内深部煤层气排采井也存在差异性。相关研究尚处于探索阶段,深入分析深部煤层气生产特征差异的影响因素对后续大规模开发阶段的产能预测与生产管理具有重要意义。【方法】为明确临兴区块深部煤层气生产特征及其影响因素,基于含气性、煤厚、埋深、水化学特征及压裂工艺等地质工程因素开展研究。【结果和结论】结果表明:(1)临兴区块深部煤层气井分为过饱和气藏自喷井、过饱和气藏排采井和欠饱和气藏排采井3类。(2)含气性是3种生产特征的主控因素,工区内1800~2000 m埋深的煤层气井含气量与稳产气量均相对较高。(3)欠饱和气藏排采井储层保存条件较差,水化学特征与其他两类井存在明显差异,导致其生产效果相对较差,且由于高矿化度和高碳酸氢根离子产生的管柱结垢与腐蚀导致排采不连续。(4)大规模压裂是提高气井产量的有效储层改造措施,压裂砂对裂缝的有效支撑是保障深部煤层气井高气量稳产的关键因素。上述成果明确了临兴区块深部煤层气生产特征差异影响因素,对研究区和邻近地区深部煤层气生产具有参考意义。

关键词：深部煤层气生产特征影响因素鄂尔多斯盆地东缘临兴区块

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鄂尔多斯盆地临兴中区盒7—盒8段有效储层物性下限及主控因素

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特种油气藏 2024年第2期31卷 66-75页

作者：赵霏王令波吴鹏徐延勇中联煤层气有限责任公司山西吕梁033200

针对临兴地区盒7—盒8段有效储层物性下限不明确的问题,基于物性数据、测井解释成果、试气资料、沉积相、铸体薄片等,综合累积频率法、采气指数法、交会图分析法开展了有效储层发育主控因素分析。研究表明:盒7、盒8段孔隙度下限值分别为... 详细信息

针对临兴地区盒7—盒8段有效储层物性下限不明确的问题,基于物性数据、测井解释成果、试气资料、沉积相、铸体薄片等,综合累积频率法、采气指数法、交会图分析法开展了有效储层发育主控因素分析。研究表明:盒7、盒8段孔隙度下限值分别为6.0%、5.5%,渗透率下限值分别为0.05、0.04 mD。有效储层受沉积、成岩、构造作用综合控制,沉积作用控制了储层的空间展布和发育规模,是影响储层物性的主要因素;成岩作用对储层物性的影响较为复杂,其中,压实作用加剧了储层致密化程度,溶蚀作用产生的次生溶孔有利于有效储层的形成,胶结作用对有效储层发育的影响具有两面性;后期构造作用有利于形成构造成因微裂缝且促进矿物溶蚀,对改善局部储层的连通性具有积极意义。该研究对于临兴地区有效储层预测以及煤系地层油气勘探具有一定的启示作用。

关键词：有效储层物性下限主控因素下石盒子组临兴中区

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从新闻标题看“央视新闻”在不同社交媒体平台的自我呈现

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新闻研究导刊 2024年第3期15卷 81-84页

作者：王凌波上海交通大学媒体与传播学院上海200240

“央视新闻”在不同社交媒体平台开设的账号所发布的新闻标题的语气、句式不尽相同。文章采用内容分析法,从新闻的软硬程度、新闻标题的情感强度以及新闻标题的互动性三个方面入手,对“央视新闻”账号在微博、微信公众平台和抖音这3个... 详细信息

“央视新闻”在不同社交媒体平台开设的账号所发布的新闻标题的语气、句式不尽相同。文章采用内容分析法,从新闻的软硬程度、新闻标题的情感强度以及新闻标题的互动性三个方面入手,对“央视新闻”账号在微博、微信公众平台和抖音这3个社交媒体平台上发布的新闻标题进行对比和分析。研究结果显示:第一,“央视新闻”微博的新闻专业性最强,“硬度”最大,互动性最弱;第二,“央视新闻”抖音号发布的新闻最“软”,新闻标题传递出的情感最强烈,互动性也最强;第三,“央视新闻”微信公众号平均标题字数最少,但指示情绪和情感表达的语气词占比最多。同样是社交媒体平台,微信公众平台更倾向于“消息发布”的媒介属性,微博具有较强的用户关系链接的特点,抖音则侧重于媒介娱乐功能。不同的平台特点,加上受众对不同社交媒体平台功能的期待,共同造就了央视新闻账号在不同平台发布新闻时,标题相异的软硬程度、情感强度和互动性。对客观的新闻陈述而言,“央视新闻”账号在不同媒体平台采用不同的自我呈现策略,使账号具备了一定的感性和个性特点,对账号的拟人化塑造方面大有裨益,也有助于构建与受众更紧密的关系。

关键词：主流媒体社交媒体自我呈现 “央视新闻” 新闻价值新闻标题

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某50CrVA弹簧钢零件断裂原因分析

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金属热处理 2024年第4期49卷 296-299页

作者：崔景博汪凌波国飞卢振国辽沈工业集团有限公司辽宁沈阳110045

采用金相显微镜、扫描电镜、氧氮氢分析仪等设备对某50CrVA弹簧钢零件断裂原因进行了分析。结果表明,50CrVA弹簧钢零件断裂均发生在直角拐角的应力集中处,经过镀锌后,由于除氢处理不完全,产生了氢脆。对此提出了改进措施,如及时更换钝... 详细信息

采用金相显微镜、扫描电镜、氧氮氢分析仪等设备对某50CrVA弹簧钢零件断裂原因进行了分析。结果表明,50CrVA弹簧钢零件断裂均发生在直角拐角的应力集中处,经过镀锌后,由于除氢处理不完全,产生了氢脆。对此提出了改进措施,如及时更换钝化的落料模具,增加除氢处理的时间。

关键词： 50CrVA弹簧钢零件应力集中氢脆模具钝化

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百日咳疑似患儿及家属百日咳鲍特菌阳性情况及菌株耐药分析

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中华微生物学和免疫学杂志 2024年第6期44卷 473-479页

作者：王凌波王华平祁正红姚苹苹滕淑吴卓颖吴蓓蓓赵仕勇杨章女浙江省疾病预防控制中心微生物检验科浙江省传染病疫苗与预防控制研究重点实验室杭州310051 浙江省杭州市儿童医院儿童感染科杭州310014

目的了解浙江省儿童疑似百日咳病例及其密接家庭成员中百日咳鲍特菌的检出情况及菌株分离率,探究百日咳鲍特菌的耐药特征及其耐药机制。方法采集2022年5—10月杭州市儿童医院收治的百日咳疑似患儿及其密接家庭成员的鼻咽拭子共273份作... 详细信息

目的了解浙江省儿童疑似百日咳病例及其密接家庭成员中百日咳鲍特菌的检出情况及菌株分离率,探究百日咳鲍特菌的耐药特征及其耐药机制。方法采集2022年5—10月杭州市儿童医院收治的百日咳疑似患儿及其密接家庭成员的鼻咽拭子共273份作为研究样本。使用木炭选择性培养基进行菌株的分离培养;荧光定量PCR法对采集样本进行百日咳靶标基因的检测;E-test法检测百日咳鲍特菌株对不同抗菌药物的敏感性;全基因组测序分析百日咳鲍特菌株对大环内酯类抗菌药物的耐药机制,并对分离的菌株进行基于核心基因组多位点序列分型(cgMLST)的进化分析。结果273株百日咳疑似患儿及其密接家庭成员样本中,荧光定量PCR阳性的样本为168份,占比61.54%,成功分离培养30株百日咳鲍特菌,阳性率为10.98%。另外,143份密接家庭成员样本中,54.55%(78/143)的样本PCR检测为阳性,9.79%(14/143)培养阳性,提示家庭成员在百日咳的家庭传播中具有重要意义,且母亲占比较高。药敏结果显示,96.67%(29/30)的菌株对大环内酯类抗菌药物阿奇霉素的MIC值>256 mg/L,显示出高度耐药性,其耐药机制均为23S rRNA发生A2047G突变。基于cgMLST的进化分析显示2022年分离的百日咳鲍特菌株已经形成两个单独的分支。结论疑似百日咳患儿的密接家庭成员中百日咳鲍特菌阳性率较高且以母亲为主,对疑似患儿家庭成员的监测及实验室检测具有重要意义;浙江省百日咳鲍特菌对大环内酯类抗菌药物呈现出高度耐药性,应进一步加强耐药性监测,为临床治疗及用药提供理论依据。

关键词：百日咳鲍特菌密接家庭成员药物耐药性耐药机制

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Enhancing leaching efficiency of ion adsorption rare earths by ameliorating mass transfer effect of rare earth ions by applying an electric field

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Journal of Rare Earths 2024年第1期42卷 172-180,I0006页

作者： lingbo Zhou Jie Yang Shijie Kang Xiaojun wang Hongdong Yu Yinhua Wan Rare Earth Institute University of Science and Technology of ChinaHefei230000China Ganjiang Innovation Academy Chinese Academy of SciencesGanzhou341000China Jiangxi Institute of Rare Earths Chinese Academy of SciencesGanzhou341000China School of Resources and Environment Engineering Jiangxi University of Science and TechnologyGanzhou341000China

Improving the seepage of leaching solution in the ore body and strengthening the mass transfer process of rare earth ions during in-situ leaching are two critical methods to improve the leaching efficiency of rare *** this study,2 wt%MgSO_(4)solution was used for the indoor simulated column leaching experiment on rare earth samples and an electric field was applied at both ends of the *** the effects of different intensities,initial application time and duration of the electric field on the rare earth leaching system and its mechanism were *** results show that compared with the single MgSO_(4)solution leaching,applying an electric field with a strength of 6 V/cm can save the leaching time of 30 min and increase the flow velocity of the rare earth leachate by 26.98%.Under the optimal conditions of applying an electric field with a strength of 6 V/cm for 20 min to the leaching system after10 min of the rare earth leachate flowing out,the leaching efficiency of sample increases from 81.20% to 86.05% with the increase of 4.85%.The mechanism analysis shows that when a direct current electric field is applied to the rare earth leaching system,rare earth ions rapidly change from disorderly movement with the seepage into faster and directional *** addition,the seepage of the leaching solution is also improved due to the increase of the cross-sectional area of the seepage channel,the polarized water molecules migrate directionally by force from the negative pole,and the movement of the hydrogen is generated by the electrolytic *** impo rtantly,based on the principle of in-situ leaching process,the layout of injection holes and deflector holes in this process provides a natural site for the electrode layout of the electric *** the simple equipme nt and the ope ration,the rare earth leaching process with the applied electric field has high feasibility in industrial application.

关键词： Ion adsorption rare earths Leaching system Electric field Mass transfer process Seepage

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Progress and Outlook of Carbon-supported Single-atom Electrocatalyst for Oxygen Reduction Reaction

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Chemical Research in Chinese Universities 2024年第3期40卷 462-474页

作者： wang Chengbin LI Ping CHEN Dehong ZHANG Ruiyong wang Lei ZONG lingbo College of Chemistry and Molecular Engineering Qingdao University of Science and TechnologyQingdao 266042P.R.China College of Materials Science and Engineering Qingdao University of Science and TechnologyQingdao 266042P.R.China Key Laboratory of Marine Environmental Corrosion and Bio-fouling Institute of OceanologyChinese Academy of SciencesQingdao 266071P.R.China

Single-atom catalysts (SACs) have garnered extensive attention in the field of catalysis due to their exceptional inherent reaction activity, optimal utilization of metal atoms, etc. Controlled synthesis plays a crucial role in elucidating the structure-activity relationship of SACs. This paper reviews various synthetic strategies for SACs, encompassing defect engineering, metal-organic frameworks (MOFs) pyrolysis, and ion exchange. With specific examples, the significance of constructing catalysts at the atomic level is discussed, aiming to comprehensively understand the synthetic strategies of SACs. Finally, it addresses the challenges and prospects associated with controlled synthesis techniques for SACs as well as their future applications.

关键词： Single-atom catalyst Synthetic strategy Carbon support Oxygen reduction reaction

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The Challenges of Global Climate Change to Green Development and its Legislative Response

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学术界 2019年第1期 223-232页

作者： wang lingbo College of Political Science and Law Jiangxi Normal University

Global climate change has swept across every nation,community,business,and individual on the planet. It is essential to find a fundamental solution to address climate change from a broader perspective. Climate change legislation should followthe principle of the sustainable development,polluter pays principle,the principle of the common but differentiated responsibilities as well as the principle of subsidiarity. Under the guidance of the four basic principles of legislation,the international field should build the legal system of the global climate governance,China should formulate special"the act of addressing climate change".

关键词： climate change green development legislative response time dimension spatial dimension

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