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Structural OH in mantle-derived clinopyroxene megacrysts from Nushan

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Chinese Science Bulletin 1998年第20期43卷 1742-1745页

作者： Qunke Xia Daogong Chen lihe guo Univ Sci & Technol China Dept Earth & Space Sci Hefei 230026 Peoples R China Chinese Acad Geol Sci Inst Mineral Deposits Beijing 100037 Peoples R China

Thirteen clinopyroxene megacrysts from Nushan Cenozoic basalts have been investigated by Fourier transform infrared spectrometer(FTIR). The results demonstrate that all samples contain trace amount of structural OH. Clinopyroxenes from Nushan show four groups of absorption bands, in which, 3 472-3 506 cm -1 is the major characteristic one. It is suggested that the position of major band is probably related to Al content: the position of major characteristic band for high_Al samples is around 3 470 cm -1 , whereas that for low_Al samples around 3 620 cm -1 .

关键词： structural OH clinopyroxene Nushan.

Monoamine alterations and rotational asymmetry in a rat model of Parkinson's disease following lateral ventricle transplantation of human amniotic epithelial cells

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Neural Regeneration Research 2009年第12期4卷 1007-1012页

作者： Shouru Xue Xinxin Yang Wanli Dong guozhen Hui lihe guo Department of Neurology First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China Department of Neurosurgery First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China Institute of Biochemistry and Cell Biology Shanghai Institute for Biological Sciences Chinese Academy of Sciences Shanghai China Shanghai Celstar Institute of Biotechnology Shanghai 201203 China

BACKGROUND： Human amniotic epithelial cells （HAECs） can differentiate into neurons, astrocytes and oligodendrocytes. They biologically secrete many active neurotrophins and have the capacity to metabolize dopamine enzymes. These features underlie a theoretical basis for the treatment of Parkinson＇s disease （PD）. OBJECTIVE： To investigate the survival and differentiation of transplanted HAECs in the lateral ventricle of PD model rats, and to explore its effect on circling behavior, as well as levels of dopamine （DA）, the metabolite homovanillic acid, dihydroxyphenyl acetic acid, 5-hydroxyindoleacetic acid, and 5-hydroxytryptamine in the striatum. DESIGN, TIME AND SETTING： A randomized, controlled, animal study was performed at the Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, and Shanghai Celstar Institute of Biotechnology from May 2007 to December 2008. MATERIALS： HAECs were derived from the placental chorion following caesarean delivery at the Shanghai International Matemal and Child Health Hospital. 6-hydroxydopamine （6-OHDA）, and mouse anti-human Vimentin monoclonal antibody were purchased from Sigma, USA; mouse anti-human nestin and tyrosine hydroxylase （TH） monoclonal antibodies were purchased from Chemicon, USA. METHODS： A total of 114 healthy, adult, Sprague Dawley rats were randomly assigned to two groups： PD model [n = 90, stereotactic microinjection of 2 μL 6-OHDA （3.5 μg/uL） into the striatum] and control （n = 24, no treatment）. The 51 successful PD model rats were randomly divided into 3 subgroups （n = 17）： HAEC, PBS, and model. The HAEC and PBS groups were respectively injected with 10 μL PBS solution containing 1 × 10^5/mL HAECs or 10 pL PBS into the lateral ventricle. The model group was not treated. MAIN OUTCOME MEASURES： TH protein expression in the striatum was evaluated by immunohistochemistry 5 weeks after HAEC transplantation. At 10 weeks, HAEC survival in the lateral ventric

关键词： human amniotic epithelial cells Parkinson's disease model lateral ventricle cell transplantation dopamine

The Discovery of Magnesioferrite from Au (Fe, Cu) Magnesian Skarn Deposits and Study of the Magnesioferrite-Magnesiomagnetite Series

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Acta Geologica Sinica(English Edition) 1998年第4期72卷 382-391页

作者： ZHAO Yiming ZHANG Yinan BI Chengsi guo lihe Institute of Mineral Deposits Chinese Academy of Geological Sciences 26 Baiwanzhuang Rd. Beijing 100037

Abstract: Magnesioferrite, a rare metasomatic mineral, was discovered for the first time in China from the Qinlou Au (Fe, Cu) magnesian skarn deposit, Sanpu, Huaibei, Auhui Province, and the Mulonggou Fe (Mo, Cu) magnesian skarn deposit, Luonan County, Shaanxi Province. In this paper, the geological setting, mineral associations, chemical composition, some physical properties, X-ray powder diffraction data and infrared spectroscopy of magnesioferrite and magnesiomagnetite are discussed. Magnesioferrite contains 17.66%–13.48% of MgO. Its main associated minerals are clinohumite, chondrodite, serpentine, calcite and magnesiomagnetite. The density of magnesioferrite is 4.537–4.720, reflectances in percent are: 17.8–18.1, hardness is 838–900 kg/mm2, and the cell parameter ao = 8.371–8.379 ?. A systematic study of the magnesioferrite-magnesiomagnetite-magnetite series suggests that along with the increase of magnesioferrite molecules in the mineral, the density, reflectances and cell parameters decrease correspondingly, the hardness heightens, and the infrared absorption spectral band becomes wider. The authors consider that magnesioferrite is a product of contact metasomatism between hypabyssal intermediate-acid intrusions and dolomitic marble. It was formed in shallow exocontact zones under relatively oxidized conditions.

关键词： magnesioferrite magnesiomagnetite Au (Fe Cu) magnesian skarn Anhui Shaanxi

Transformation of human amniotic epithelial cells into neuron-like cells in the microenvironment of traumatic brain injury in vivo and in vitro

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Neural Regeneration Research 2011年第10期6卷 744-749页

作者： Yi Lu guozhen Hui Zhiyuan Wu Tianjin Liu Xunhe Ji lihe guo Department of Neurosurgery First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China Department of Neurosurgery First Hospital of Jiaxing City Jiaxing 314000 Zhejiang Province China Institute of Biochemistry and Cell Biology Shanghai Institute of Life Sciences Chinese Academy of Sciences Shanghai 200031 China

Survival and differentiation of transplanted cells is closely related to the local *** present study cultured human amniotic epithelial cells（HAECs） in a simulated microenvironment in vitro comprising RPMI 1640 culture medium and the solution extracted from injured brain *** HAECs were round,triangular in form or irregularly shaped,with extended neuron-like processes;some of the processes were interconnected,representing neuron-like morphology and some HAECs were microtubule-associated protein *** survived for at least 4 weeks following transplantation into the center and edges of the trauma focus with traumatic brain injury,and were microtubule-associated protein ***,the motor function of rat hind limbs was significantly improved.

关键词： traumatic brain injury microenvironment transplantation human amniotic epithelialcells neural cell differentiation neural regeneration

Water in Mantle-derived Xenoliths in the Hannuoba Basalt

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Acta Geologica Sinica(English Edition) 1998年第2期72卷 224-229页

作者： guo lihe LIN Xingyuan XIE Manze FENG Jialin WU Shuqi Institute of Mineral Deposits CAGS26 BaiwanzhuangBeijing 100037 Shijiazhuang Economic Institute ShijiazhuangHebei 050031 Institute of Rock and Mineral Analysis Beijing 100037

: Minerals of various mantle-derived xenoliths from the Hannuoba basalt in Hebei Province have been studied by means of IR spectroscopy. The results show that all xenoliths from the mantle at depths <75 km contain trace amounts of water (0.45%–11.6×10?2% H2O). The data of about 0.1% H2O contained in primary pyrolite estimated by earlier studies may be on the high side. The water might enter the frameworks of olivine, pyroxene and garnet earlier than it entered those of amphibole and phlogopite. The presence of water in amphibole and phlogopite may be a local phenomenon of water enrichment, which is related to relatively small-scale magmatic or metasomatic events although they can contain a hundred times more water than pyroxene contains. There is a little more water (1.11%–3.01×10?2% of H2O mostly) in xenoliths from the Hannuoba basalt than in those from mid-ocean ridge basalt and kimberlites of South Africa (less than 1×10?2% of H2O mostly). This indicates the heterogeneity of water in time and space in the upper mantle. The presence of trace amounts of water in mantle-derived anhydrous silicate minerals provides basic data for us to understand the distribution of water in the upper mantle and the role of water in the mantle dynamic and geochemical processes.

关键词： mantle fluids fluid geochemistry water in minerals Hannuoba Hebei

Identification of Ser^(354) and Ser^(357) involved in the function of norepinephrine transporter

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Chinese Science Bulletin 1998年第18期43卷 1541-1544页

作者： HUANG Fang LIU Yanhong FEI Jian guo lihe Wolfgang Schwarz Shanghai Institute of Cell Biology Chinese Academy of SciencesShanghai 200031China Max-Planck Institute for Biophysik Kennedy Allee 7060956 Frankfurt/MainGermany

Conserved Ser 354, Ser 357 in the seventh transmembrane domain of NET were mutated and the function of mutants was studied by 3H-NE influx measurement. Double mutation at these two serine sites reduced the activity of NET to base level. It is inferred that these two sites may be involved in the recognition and transport activity of the NET protein.

关键词： norepinephrine transporter mutants.

Human amniotic epithelial cells express specific markers of nerve cells and migrate along the nerve fibers in the corpus callosum

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Neural Regeneration Research 2012年第1期7卷 41-45页

作者： Zhiyuan Wu guozhen Hui Yi Lu Tianjin Liu Qin Huang lihe guo Department of Neurosurgery Changzhou Second People＇s Hospital Nanjing Medical University Nanjing 213000 Jiangsu Province China Department of Neurosurgery the First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Human amniotic epithelial cells were isolated from a piece of fresh amnion. Using immunocytochemical methods, we investigated the expression of neuronal phenotypes （microtubule-associated protein-2, glial fibrillary acidic protein and nestin） in human amniotic epithelial cells. The conditioned medium of human amniotic epithelial cells promoted the growth and proliferation of rat glial cells cultured in vitro, and this effect was dose-dependent. Human amniotic epithelial cells were further transplanted into the corpus striatum of healthy adult rats and the grafted cells could integrate with the host and migrate 1 2 mm along the nerve fibers in corpus callosum. Our experimental findings indicate that human amniotic epithelial cells may be a new kind of seed cells for use in neurograft.

关键词： human amniotic epithelial cells cell transplantation microtubule-associated protein-2 glial fibrillary acidic protein nestin neurotrophin central nervous system injury neural regeneration

Tribological behavior of shape memory cyanate ester materials and their tunable friction mechanism

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Friction 2023年第10期11卷 1794-1803页

作者： Zhangzhang TANG lihe guo Mingkun XU Hongwei RUAN Jing YANG Tingmei WANG Jianqiang ZHANG Qihua WANG Yaoming ZHANG Key Laboratory of Science and Technology on Wear and Protection of Materials Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China College of Petrochemical Technology Lanzhou University of TechnologyLanzhou 730050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

High-performance polymer friction materials with tunable tribological behavior to fit varied work conditions remain a challenge of widespread interest for a variety of *** memory polymer exhibits morphing and modulus changing over temperature changing provides a promising material to adjust the friction ***,we investigated the tribological properties of shape memory cyanate ester(SMCE)under different *** SMCE exhibits the tribological behavior of good friction material with stable high coefficient of friction(COF)and a low wear ***,the COF increases and wear rate decreases with the temperature increasing show the tunable friction property of the *** propose a new model of wear-compensation through shape recovery to explain the adjustable friction behavior of thermal-responsive polymer from the aspect of shape recovery and energy *** study provides a high-performance friction material and paves the route for the application of shape memory polymer(SMP)in tribology field with tunable property.

关键词： tribological behavior shape memory polymer cyanate ester tunable friction wear rate

Studies of substrate binding region of mEAAC1 and mASCT1 by constructing chimeras

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Chinese Science Bulletin 1999年第6期44卷 524-528页

作者： Jing Li Jibin Peng Jian Fei Fang Huang Quanbao Gu lihe guo Chinese Acad Sci Shanghai Inst Cell Biol Mol Cell Biol Lab Shanghai 200031 Peoples R China

The cDNA chimeras between two subtypes of mouse excitatory amino acid transporter family, mouse excitatory amino acid carrier 1 （mEAAC1） and mouse alanine serine cysteine transporter 1 （mASCT1）, were constructed by recombinant PCR. After transcription in vitro, the cRNA was injected and expressed in Xenopus laevis oocytes. 3H-Glu and 3H-Ser were used as isotopic tracer to measure the flux of amino acids. The results showed that there might not be the key amino acids responsible for substractive specificity in the NH2-terminal and its adjacent regions of these two transporters, which probably supported the formation of the substrata binding sites.

关键词： mEAAC1 mASCT1 chimeras substrate binding region.