Human amniotic epithelial cells express specific markers of nerve cells and migrate along the nerve fibers in the corpus callosum

作     者：Zhiyuan Wu Guozhen Hui Yi Lu Tianjin Liu Qin Huang Lihe Guo 

作者机构：Department of Neurosurgery Changzhou Second People＇s Hospital Nanjing Medical University Nanjing 213000 Jiangsu Province China Department of Neurosurgery the First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2012年第7卷第1期

页      面：41-45页

核心收录：

学科分类：0905[农学-畜牧学] 08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：sponsored by the National Natural Science Foundation of China, No. 30271325 the Natural Science Foundation of Jiangsu Province, No. BK2001170 the National Basic Research Program of China (973 Program), No.2005CB522604 

主　　题：human amniotic epithelial cells cell transplantation microtubule-associated protein-2 glial fibrillary acidic protein nestin neurotrophin central nervous system injury neural regeneration 

摘      要：Human amniotic epithelial cells were isolated from a piece of fresh amnion. Using immunocytochemical methods, we investigated the expression of neuronal phenotypes （microtubule-associated protein-2, glial fibrillary acidic protein and nestin） in human amniotic epithelial cells. The conditioned medium of human amniotic epithelial cells promoted the growth and proliferation of rat glial cells cultured in vitro, and this effect was dose-dependent. Human amniotic epithelial cells were further transplanted into the corpus striatum of healthy adult rats and the grafted cells could integrate with the host and migrate 1 2 mm along the nerve fibers in corpus callosum. Our experimental findings indicate that human amniotic epithelial cells may be a new kind of seed cells for use in neurograft.