Transformation of human amniotic epithelial cells into neuron-like cells in the microenvironment of traumatic brain injury in vivo and in vitro

作     者：Yi Lu Guozhen Hui Zhiyuan Wu Tianjin Liu Xunhe Ji Lihe Guo 

作者机构：Department of Neurosurgery First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China Department of Neurosurgery First Hospital of Jiaxing City Jiaxing 314000 Zhejiang Province China Institute of Biochemistry and Cell Biology Shanghai Institute of Life Sciences Chinese Academy of Sciences Shanghai 200031 China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2011年第6卷第10期

页      面：744-749页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：the Grant from National Basic Research Program of China (973 Program) for Traumatic Projects  No. 2005CB522604 

主　　题：traumatic brain injury microenvironment transplantation human amniotic epithelialcells neural cell differentiation neural regeneration 

摘      要：Survival and differentiation of transplanted cells is closely related to the local *** present study cultured human amniotic epithelial cells（HAECs） in a simulated microenvironment in vitro comprising RPMI 1640 culture medium and the solution extracted from injured brain *** HAECs were round,triangular in form or irregularly shaped,with extended neuron-like processes;some of the processes were interconnected,representing neuron-like morphology and some HAECs were microtubule-associated protein *** survived for at least 4 weeks following transplantation into the center and edges of the trauma focus with traumatic brain injury,and were microtubule-associated protein ***,the motor function of rat hind limbs was significantly improved.