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GIGANTEA orthologs,E2 members,redundantly determine photoperiodic flowering and yield in soybean

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Journal of Integrative Plant Biology 2023年第1期65卷 188-202页

作者： Lingshuang Wang Haiyang Li Milan He Lidong Dong Zerong Huang Liyu Chen Haiyang Nan Fanjiang Kong Baohui Liu Xiaohui Zhao Guangdong Key Laboratory of Plant Adaptation and Molecular Design Guangzhou Key Laboratory of Crop Gene EditingInnovative Center of Molecular Genetics and EvolutionSchool of Life Sciences Guangzhou UniversityGuangzhou 510006 China National Key Laboratory of Crop Genetics and Germplasm Enhancement National Center for Soybean ImprovementJiangsu Collaborative Innovation Center for Modern Crop Production Nanjing Agricultural UniversityNanjing 210095 China

Soybean(Glycine max L.)is a typical photoperiodsensitive crop,such that photoperiod determines its flowering time,maturity,grain yield,and phenological adaptability.During evolution,the soybean genome has undergone two duplication events,resulting in about 75%of all genes being represented by multiple copies,which is associated with rampant gene redundancy.Among duplicated genes,the important soybean maturity gene E2 has two homologs,E2-Like a(E2La)and E2-Like b(E2Lb),which encode orthologs of Arabidopsis GIGANTEA(GI).Although E2 was cloned a decade ago,we still know very little about its contribution to flowering time and even less about the function of its homologs.Here,we generated single and double mutants in E2,E2La,and E2Lb by genome editing and determined that E2 plays major roles in the regulation of flowering time and yield,with the two E2 homologs depending on E2 function.At high latitude regions,e2 single mutants showed earlier flowering and high grain yield.Remarkably,in terms of genetic relationship,genes from the legume-specific transcription factor family E1 were epistatic to E2.We established that E2 and E2-like proteins form homodimers or heterodimers to regulate the transcription of E1 family genes,with the homodimer exerting a greater function than the heterodimers.In addition,we established that the H3 haplotype of E2 is the ancestral allele and is mainly restricted to low latitude regions,from which the loss-of-function alleles of the H1 and H2haplotypes were derived.Furthermore,we demonstrated that the function of the H3 allele is stronger than that of the H1 haplotype in the regulation of flowering time,which has not been shown before.Our findings provide excellent allelic combinations for classical breeding and targeted gene disruption or editing.

关键词： E2 E2-Like flowering time GIGANTEA natural variation redundancy yield

Agronomical selection on loss-of-function of GIGANTEA simultaneously facilitates soybean salt tolerance and early maturity

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Journal of Integrative Plant Biology 2022年第10期64卷 1866-1882页

作者： Lidong Dong Zhihong Hou Haiyang Li Zhaobo Li Chao Fang Lingping Kong Yongli Li Hao Du Tai Li Lingshuang Wang Milan He Xiaohui Zhao Qun Cheng Fanjiang Kong Baohui Liu Guangdong Key Laboratory of Plant Adaptation and Molecular Design Guangzhou Key Laboratory of Crop Gene EditingInnovative Center of Molecular Genetics and EvolutionSchool of Life SciencesGuangzhou UniversityGuangzhou Higher Education Mega Center230 Waihuanxi RoadGuangzhou 510006China The Innovative Academy of Seed Design Key Laboratory of Soybean Molecular Design BreedingNortheast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081China College of Agriculture Heilongjiang Bayi Agricultural UniversityDaqingChina National Key Laboratory of Crop Genetics and Germplasm Enhancement National Center for Soybean ImprovementJiangsu Collaborative Innovation Center for Modern Crop ProductionNanjing Agricultural UniversityNanjing 210095China College of Agriculture Jilin Agricultural Science and Technology UniversityJilin 132101China

Salt stress and flowering time are major factors limiting geographic adaptation and yield productivity in soybean(Glycine max).Although improving crop salt tolerance and latitude adaptation are essential for efficient agricultural production,whether and how these two traits are integrated remains largely unknown.Here,we used a genome-wide association study to identify a major salt-tolerance locus controlled by E2,an ortholog of Arabidopsis thaliana GIGANTEA(GI).Loss of E2 function not only shortened flowering time and maturity,but also enhanced salt-tolerance in soybean.E2 delayed soybean flowering by enhancing the transcription of the core flowering suppressor gene E1,thereby repressing Flowering Locus T(FT)expression.An E2 knockout mutant e2^(CR) displayed reduced accumulation of reactive oxygen species(ROS)during the response to salt stress by releasing peroxidase,which functions in ROS scavenging to avoid cytotoxicity.Evolutionary and population genetic analyses also suggested that loss-of-function e2 alleles have been artificially selected during breeding for soybean adaptation to high-latitude regions with greater salt stress.Our findings provide insights into the coupled selection for adaptation to both latitude and salt stress in soybean;and offer an ideal target for molecular breeding of early-maturing and salt-tolerant cultivars.

关键词： floweringtime GIGANTEA ROS saltstress soybean

Visible-light deposition of CrO_(x) cocatalyst on TiO_(2):Cr valence regulation for superior photocatalytic CO_(2)reduction to CH_(4)

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Journal of Energy Chemistry 2022年第1期31卷 103-112,I0004页

作者： Jingjing dong Yuan Kong Heng Cao Zhiyu Wang Zhirong Zhang lidong Zhang Song Sun Chen Gao Xiaodi Zhu Jun Bao National Synchrotron Radiation Laboratory Collaborative Innovation Center of Chemistry for Energy MaterialsUniversity of Science and Technology of ChinaHefei 230029AnhuiChina Hefei National Laboratory for Physical Science at the Microscale and Department of Chemical Physics Synergetic Innovation Center of Quantum Information and Quantum PhysicsUniversity of Science and Technology of ChinaHefei 230026AnhuiChina School of Chemistry and Chemical Engineering Anhui UniversityHefei 230601AnhuiChina School of Physical Science University of Chinese Academy of SciencesBeijing 100049China

Photodeposition is widely adopted for implanting metal/metal oxide cocatalysts on semiconductors.However,it is prerequisite that the photon energy should be sufficient to excite the host semiconductor.Here,we report a lower-energy irradiation powered deposition strategy for implanting CrO_(x) cocatalyst on TiO_(2).Excitingly,CrO_(x)-400 implanted under visible-light irradiation significantly promotes the CH4 evolution rate on TiO_(2)to 8.4μmolg·^(-1)h^(-1) with selectivity of98%from photocatalytic CO_(2)reduction,which is 15 times of that on CrO_(x)-200 implanted under UV-visible-light irradiation.Moreover,CrO_(x)-400 is identified to be composed of higher valence Cr species compared to CrO_(x)-200.This valence states regulation of Cr species is indicated to provide more active sites for CO_(2) adsorption/activation and to modulate the reaction mechanism from single Cr site to Cr-Cr dual sites,thus endowing the superior CH_(4)production.This work demonstrates an alternative strategy for constructing efficient metal oxides cocatalysts on wide bandgap semiconductor.

关键词： Valence states regulation CrO_(x)cocatalyst Lower-energy irradiation Photocatalytic CO_(2)reduction High CH_(4)selectivity

Soybean AP1 homologs control flowering time and plant height

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Journal of Integrative Plant Biology 2020年第12期62卷 1868-1879页

作者： Liyu Chen Haiyang Nan Lingping Kong Lin Yue Hui Yang Qingsong Zhao Chao Fang Haiyang Li Qun Cheng Sijia Lu Fanjiang Kong Baohui Liu Lidong Dong Innovative Center of Molecular Genetics and Evolution School of Life SciencesGuangzhou UniversityGuangzhou 510006China The Key Laboratory of Crop Genetics and Breeding of Hebei Institute of Cereal and Oil CropsHebei Academy of Agricultural and ForestrySciencesShijiazhuang 050000China National Key Laboratory of Crop Genetics and Germplasm Enhancement Jiangsu Collaborative Innovation Center for Modern CropProductionNanjing Agricultural UniversityNanjing 210000China The Innovative Academy of Seed Design Key Laboratory of Soybean Molecular Design BreedingNortheast Institute of Geography andAgroecologythe Chinese Academy of SciencesHarbin 150000China

Flowering time and plant height are key agronomic traits that directly affect soybean(Glycine max)yield.APETALA1(AP1)functions as a class A gene in the ABCE model for floral organ development,helping to specify carpel,stamen,petal,and sepal identities.There are four AP1 homologs in soybean,all of which are mainly expressed in the shoot apex.Here,we used clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein 9 technology to generate a homozygous quadruple mutant,gmap1,with loss-of-function mutations in all four GmAP1 genes.Under short-day(SD)conditions,the gmap1 quadruple mutant exhibited delayed flowering,changes in flower morphology,and increased node number and internode length,resulting in plants that were taller than the wild type.Conversely,overexpression of GmAP1a resulted in early flowering and reduced plant height compared to the wild type under SD conditions.The gmap1 mutant and the overexpression lines also exhibited altered expression of several genes related to flowering and gibberellic acid metabolism,thereby providing insight into the role of GmAP1 in the regulatory networks controlling flowering time and plant height in soybean.Increased node number is the trait with the most promise for enhancing soybean pod number and grain yield.Therefore,the mutant alleles of the four AP1 homologs described here will be invaluable for molecular breeding of improved soybean yield.

关键词： soybean breeding height

A recent retrotransposon insertion of J caused E6 locus facilitating soybean adaptation into low latitude

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Journal of Integrative Plant Biology 2021年第6期63卷 995-1003页

作者： Chao Fang Jun Liu Ting Zhang Tong Su Shichen Li Qun Cheng Lingping Kong Xiaoming Li Tiantian Bu Haiyang Li Lidong Dong Sijia Lu Fanjiang Kong Baohui Liu Innovative Center of Molecular Genetics and Evolution School of Life SciencesGuangzhou UniversityGuangzhou 510006China The Innovative Academy of Seed Design Key Laboratory of Soybean Molecular Design BreedingNortheast Institute of Geography and Agroecologythe Chinese Academy of SciencesHarbin 150000China Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement South China Botanical Gardenthe Chinese Academy of SciencesGuangzhou 510650China National Key Laboratory of Crop Genetics and Germplasm Enhancement Jiangsu Collaborative Innovation Center for Modern Crop ProductionNanjing Agricultural UniversityNanjing 210095China

Soybean(Glycine max) is an important legume crop that was domesticated in temperate regions.Soybean varieties from these regions generally mature early and exhibit extremely low yield when grown under inductive short-day(SD) conditions at low latitudes. The long-juvenile(LJ) trait, which is characterized by delayed flowering and maturity,and improved yield under SD conditions, allowed the cultivation of soybean to expand to lower latitudes. Two major loci control the LJ trait: J and E6. In the current study, positional cloning, sequence analysis, and transgenic complementation confirmed that E6 is a novel allele of J, the ortholog of Arabidopsis thaliana EARLY FLOWERING 3(ELF3). The mutant allele e6^(PG), which carries a Ty1/Copia-like retrotransposon insertion, does not suppress the legume-specific flowering repressor E1, allowing E1 to inhibit Flowering Locus T(FT) expression and thus delaying flowering and increasing yields under SD conditions. The e6^(PG)allele is a rare allele that has not been incorporated into modern breeding programs.The dysfunction of J might have greatly facilitated the adaptation of soybean to low latitudes. Our findings increase our understanding of the molecular mechanisms underlying the LJ trait and provide valuable resources for soybean breeding.

关键词： E6 long-juvenile(LJ)trait J Ty1/copia-like retrotransposon

Electroacupuncture diminishes P2X_2 and P2X_3 purinergic receptor expression in dorsal root ganglia of rats with visceral hypersensitivity

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Neural Regeneration Research 2013年第9期8卷 802-808页

作者： Zhijun Weng Luyi Wu Yuan Lu lidong Wang Linying Tan Ming dong Yuhu Xin Shanghai Academy of Traditional Chinese Medicine Fudan University Shanghai Cancer Center

Electroacupuncture at Shangjuxu （ST37） and Tianshu （ST25） can improve visceral hypersensitivity in rats. Colorectal distension was used to establish a rat model of chronic visceral hypersensitivity. Immunohistochemistry was used to detect P2X2 and P2X3 receptor expression in dorsal root ganglia from rats with chronic visceral hypersensitivity. Results demonstrated that abdominal withdrawal reflex scores obviously increased following establishment of the model, indicating visceral hypersensitivity. Simultaneously, P2X2 and P2X3 receptor expression increased in dorsal root ganglia. After bilateral electroacupuncture at Shangjuxu and Tianshu, abdominal withdrawal reflex scores and P2X2 and P2X3 receptor expression decreased in rats with visceral hypersensitivity. These results indicated that electroacupuncture treatment improved visceral hypersensitivity in rats with irritable bowel syndrome by reducing P2X2 and P2X3 receptor expression in dorsal root ganglia.

关键词： neural regeneration acupuncture and moxibustion P2X2 P2X3 visceral hypersensitivity irritablebowel syndrome electroacupuncture P2 purinergic receptors abdominal withdrawal reflex scoresacupuncture and moxibustion peripheral nerve injury grants-supported paper photographscontaining paper neuroregeneration

Cone Barrel Detection of Driverless Racing Car Based on Lidar

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Cone Barrel Detection of Driverless Racing Car Based on Lida...

A Novel Pathogenesis-Related Class 10 Protein Glym41,Increases Resistance upon Phytophthora sojae Infection in Soybean(Glycine max[L.]Merr.)

2022中国汽车工程学会年会暨展览会

作者： Gong Yanqiao Li Gang Li Xiaogang Liu lidong Zhang dong Automobile And Traffic Engineering Liaoning University of Technology Lincoln University Brunel University

In the key technology of driverless formula racing environment perception,the quality of real-time perception effect is very important to the operation of subsequent algorithms.This paper studies a cone barrel detection algorithm based on lidar.Firstly,the lidar is calibrated by three-point collinear and plane fitting methods,and then the original laser point cloud data is preprocessed,followed by ground segmentation,point cloud clustering,feature extraction and other steps,Finally,the position information of the cone barrel is obtained.The algorithm is deployed in ROS for real vehicle test.The accuracy and sensitivity of the experimental results are higher than 90%,and the single frame processing time is about 50 ms.The results show that the algorithm can accurately identify the cone barrel and has good real-time performance.

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A Novel Pathogenesis-Related Class 10 Protein Glym41,Increas...

Overexpression of GmERF5,a new member of the soybean EAR motif-containing ERF transcription factor,enhances resistance to Phytophthora sojae in soybean

中国作物学会——2015年学术年会

作者： Sujie Fan Liangyu Jiang Lidong Dong Pengfei Xu Shuzhen Zhang Soybean Research Institute Northeast Agricultural University

Phytophthora root and stem rot of soybean,caused by Phytophthora sojae(P.sojae),is a destructive disease in many soybean planting regions worldwide.In a previous study,an expressed sequence tag(EST)homolog of the major allergen Pru ar 1 in apricot(Prunus armeniaca)was identified up-regulated in the highly resistant soybean’Suinong 10’infected with P.sojae.Here,the full length of the EST was isolated using rapid amplification of cDNA ends(RACE).It showed the highest homolgy of 53.46%with Gly m 4after comparison with the eight soybean allergen families reported and was named Gly m 4-like(Gly m 4l,GenBank accession no.HQ913577.1).The cDNA full length of Gly m 4l was 707 bp containing a 474 bp open reading frame encoding a polypeptide of 157 amino acids.Sequence analysis suggests that Gly m 41contains a conserved’P-loop’(phosphate-binding loop)motif at residues 47-55 aa and a Bet v 1 domain at residues 87-120 aa.The transcript abundance of Gly m 4l was significantly induced by P.sojae,salicylic acid(SA),NaCl,and also responded to methyl jasmonic acid(MeJA)and ethylene(ET).The recombinant Gly m 41 protein showed RNase activity and displayed directly antimicrobial activity that inhibited hyphal growth and reduced zoospore release in P.sojae.Further analyses showed that the RNase activity of the recombinant protein to degrading tRNA was significantly affected in the presence of zeatin.Over-expression of Gly m 4l in susceptible’dongnong 50’soybean showed enhanced tolerance to P.sojae.These results indicated that Gly m 41 protein played an important role in the defense of soybean against P.sojae infection.

关键词： Glycine max Phytophthora sojae Pathogenesis-Related Class 10 Protein

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Overexpression of GmERF5,a new member of the soybean EAR mot...