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Designing artificial synthetic promoters for accurate,smart,and versatile gene expression in plants

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Plant Communications 2023年第4期4卷 12-29页

作者： Erum Yasmeen Jin Wang Muhammad Riaz lida zhang Kaijing Zuo Single Cell Research Center School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijingChina

With the development of high-throughput biology techniques and artificial intelligence,it has become increasingly feasible to design and construct artificial biological parts,modules,circuits,and even whole *** overcome the limitations of native promoters in controlling gene expression,artificial promoter design aims to synthesize short,inducible,and conditionally controlled promoters to coordinate the expression of multiple genes in diverse plant metabolic and signaling *** promoters are versatile and can drive gene expression accurately with smart responses;they show potential for enhancing desirable traits in crops,thereby improving crop yield,nutritional quality,and food *** review first illustrates the importance of synthetic promoters,then introduces promoter architecture and thoroughly summarizes advances in synthetic promoter *** to the development of synthetic promoters and future applications of such promoters in synthetic plant biology and crop improvement are also discussed.

关键词： plants synthetic promoter cis element gene expression

Targeted mutagenesis in Arabidopsis and medicinal plants using transposon-associated TnpB^(FA)

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Journal of Integrative Plant Biology 2024年第10期66卷 2083-2086页

作者： Zongyou Lv Wenhua Chen Shiyuan Fang Boran Dong Xingxing Wang lida zhang Jingshi Xue Wansheng Chen Research and Development Center of Chinese Medicine Resources and Biotechnology Shanghai University of Traditional Chinese MedicineShanghai 201203China Department of Plant Science School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghaiChina Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 201203China Department of Pharmacy Changzheng HospitalSecond Military Medical UniversityShanghai 200003China

Medicinal plants produce valuable compounds, but often at low concentrations. Genome editing could be used to increase the production of valuable secondary metabolites in medicinal plants. The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9(Cas9) system has emerged as a simple, widely used method for gene editing in plants.

关键词： CRISPR Genome Arabidopsis

A Fractal Orifice-Throat Model for Seepage Characteristics of Multiscale Porous Media

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Journal of Mechanics Engineering and Automation 2023年第5期13卷 136-142页

作者： Chunling Wang Yan Gao lida zhang Jiaoyan Zhu College of Science China Jiliang UniversityHangzhou 310018China

The seepage characteristics of multiscale porous media is of considerable significance in many scientific and engineering *** Darcy permeability is one of the key macroscopic physical properties to characterize the seepage capacity of porous ***,based on the statistically fractal scaling law of porous media,fractal geometry is applied to model the multiscale pore *** a two-dimensional fractal orifice-throat model with multiscale and tortuous characteristics is proposed for the seepage flow through porous *** analytical expression for Darcy permeability of porous media is derived,which is validated by comparing with available experimental *** results show that the Darcy permeability is significantly influenced by porosity,orifice-throat fractal dimension,minimum to maximum diameter ratio,orifice-throat ratio and tortuosity fractal *** present results are helpful for understanding the seepage mechanism of multiscale porous media,and may provide theoretical basis for unconventional oil and gas exploration and development,porous phase transition energy storage composites,CO2 geological sequestration,environmental protection and nuclear waste treatment,etc.

关键词： Multiscale porous media fractal geometry Darcy’s law permeability orifice-throat model.

Functional Analysis of a Wilt Fungus Inducible <i>PR</i>10-1 Gene from Cotton

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American Journal of Plant Sciences 2013年第2期4卷 417-426页

作者： Lijun Chen Na Sun Jin Wang Hua Ling lida zhang Kaijing Zuo Plant Biotechnology Center Fudan-SJTU-Nottingham Plant Biotechnology R&D Center College of Agriculture and Life Science Shanghai Jiao Tong University Shanghai China Plant Genomics Research Center Mianyang Normal University Mianyang China

Early stage expression of PR10 combined with phytoalexins contributed to Verticillium wilt resistance in cotton. In order to analysis the activities of PR10 proteins during pathogens’ infection, we cloned a Verticillium-induced PR10 (GbPR10-1) gene from cotton (Gossypium barbadense) and compared its expression patterns and domains with other PR10 proteins. Bioinformatics indicated that GbPR10-1 showed the lowest similarity with other 12 different PR10 genes in cotton (Upland and sea-island cotton). Expression profiles showed that GbPR10-1 gene instantly up-regulated after infection by V. dahliae in the sea-island cotton plants. GbPR10-1 was also induced by environmental stimulus including heat, submergence and salt, and ethylene but not by ABA and salicylic acid. The GbPR10-1 protein expressed in E. coli BL21 demonstrated that it had a low ribonuclease-like activity in vitro, and could inhibit V. dahliae hyphae growth but not its spores. Comparison analysis of GbPR10-1 (from resistant species) and GhPR10-1 (from susceptible species) responding to V. dahliae infection, only GbPR10-1 gene was strongly induced in the sea-island cotton plants (incompatible response), indicating that PR10-1 genes was linked to resistance signal. In summary, the earlier activation of GbPR10-1 gene, as the index of resistance response, would be aid to block

关键词： Pathogenesis-Related Protein Gossypium barbadense Ribonuclease Activity Incompatible Response

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文化哲学视野下的文化变迁机制

学术交流 2017年第3期 35-42页

作者：张立达西南政法大学马克思主义学院重庆401120

在文化哲学视野下研究文化变迁机制,应该聚焦于作为精神体系的狭义文化,从一般形式层面把握意义体验机制中稳定性和变易性的辩证关系。价值理性是维系文化认同的稳定性因素,工具理性和价值理性辩证统一而产生的对实践自由的追求则是推... 详细信息

在文化哲学视野下研究文化变迁机制,应该聚焦于作为精神体系的狭义文化,从一般形式层面把握意义体验机制中稳定性和变易性的辩证关系。价值理性是维系文化认同的稳定性因素,工具理性和价值理性辩证统一而产生的对实践自由的追求则是推动文化变迁的变易性因素。意义认同可以区分出从具体到抽象的不同层次,因此具体的变易仍可以包容在原有的较抽象的意义框架中,由此变与不变的辩证统一才使变化得以现实的发生。意义认同向抽象层次的提升,从而更好地包容人的自由创造,正是文化进步的实质。最后从方法论层面来看,经马克思主义批判改造的现象学方法,是真正理解人与文化所必须采用的方法。

关键词：文化变迁机制工具理性价值理性自由意义现象学

The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis

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Molecular Plant 2018年第6期11卷 776-788页

作者： Qian Shen lida zhang Zhihua Liao Shengyue Wang Tingxiang Yan Pu Shi Meng Liu Xueqing Fu Qifang Pan Yuliang Wang Zongyou Lv Xu Lu Fangyuan zhang Weimin Jiang Yanan Ma Minghui Chen Xiaolong Hao Ling Li Yueli Tang Gang Lv Yan Zhou Xiaofen Sun Peter E. Brodelius Jocelyn K.C. Rose Kexuan Tang Joint International Research Laboratory of Metabolic & Developmental Sciences Key Laboratory of Urban Agriculture (South) Ministry of Agriculture Plant Biotechnology Research Center Fudan-SJTU-Nottingham Plant Biotechnology R&D Center School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200240 China SWU-TAAHC Medicinal Plant Joint R&D Centre School of Life Sciences Southwest University Chongqing 400715 China Chinese National Human Genome Center at Shanghai Shanghai 201203 China Plant Biology Section School of Integrative Plant Science Cornell University Ithaca NY 14853 USA Department of Chemistry and Biomedical Sciences Linnaeus University 39182 Kalmar Sweden State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing 211198 China

Artemisia annua, commonly known as sweet wormwood or Qinghao, is a shrub native to China and has long been used for medicinal purposes. A. annua is now cultivated globally as the only natural source of a potent anti-malarial compound, artemisinin. Here, we report a high-quality draft assembly of the 1.74-gigabase genome of A. annua, which is highly heterozygous, rich in repetitive sequences, and contains 63 226 protein-coding genes, one of the largest numbers among the sequenced plant species. We found that, as one of a few sequenced genomes in the Asteraceae, the A. annua genome contains a large number of genes specific to this large angiosperm clade. Notably, the expansion and functional diversification of genes encoding enzymes involved in terpene biosynthesis are consistent with the evolution of the artemi- sinin biosynthetic pathway. We further revealed by transcriptome profiling that A. annua has evolved the sophisticated transcriptional regulatory networks underlying artemisinin biosynthesis. Based on compre- hensive genomic and transcriptomic analyses we generated transgenic A. annua lines producing high levels of artemisinin, which are now ready for large-scale production and thereby will help meet the chal- lenge of increasing global demand of artemisinin.

关键词： Artemisia annua artemisinin genome evolution transcriptome metabolic engineering

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基于模糊理论的风力机故障诊断专家系统构建

机械科学与技术 2007年第5期26卷 581-584页

作者：任腊春张礼达西华大学能源与环境学院成都610039

目前,对风力机的故障进行诊断仅停留在人工现场诊断的层面上,使得故障难以及时发现并排除。该文以模糊数学理论和故障诊断技术为基础,建立一个用于风力机故障诊断的专家系统。文中首先介绍了模糊综合评判方法;然后给出了诊断专家系统的... 详细信息

目前,对风力机的故障进行诊断仅停留在人工现场诊断的层面上,使得故障难以及时发现并排除。该文以模糊数学理论和故障诊断技术为基础,建立一个用于风力机故障诊断的专家系统。文中首先介绍了模糊综合评判方法;然后给出了诊断专家系统的结构,其中重点论述了如何根据经验数据和专家优先系数法共同确定模糊关系矩阵的元素、模糊诊断原则的确定及推理流程。最后用一个具体的故障诊断实例进行分析,验证了应用模糊理论,可以提高风力机故障诊断专家系统的运行速度、准确性、可靠性。

关键词：风力机故障诊断专家系统模糊理论

基于模糊理论的水轮机导水机构故障诊断方法的研究

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水力发电 2008年第3期34卷 62-64页

作者：王旭张礼达西华大学能源与环境学院四川成都610039

构建水轮机导水机构故障诊断系统的目的,是自动诊断得出故障发生的原因。为此,以模糊理论为基础,结合水轮机导水机构的实际运行工况、专家经验等,构建了导水机构故障诊断知识库;分析了故障原因与故障征兆之间的模糊关系,建立了导水机构... 详细信息

构建水轮机导水机构故障诊断系统的目的,是自动诊断得出故障发生的原因。为此,以模糊理论为基础,结合水轮机导水机构的实际运行工况、专家经验等,构建了导水机构故障诊断知识库;分析了故障原因与故障征兆之间的模糊关系,建立了导水机构模糊故障诊断数学模型。用故障诊断实例验证了模糊理论在水轮机导水机构故障诊断中的可行性。

关键词：模糊理论导水机构故障诊断

审计风险联结是否影响关键审计事项披露行为?——基于“低质量审计传染”问题的情境研究

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审计研究 2024年第4期 65-76页

作者：张丽达冯均科王艺西安财经大学商学院 710100 西北大学经济管理学院 710127

低质量审计传染对于审计师与上市公司具有负面影响。本文采取2016-2022年我国A股上市公司的数据,基于财务重述事件中错报年时,上市公司之间因相同签字审计师产生的审计师联结,将重述年时被联结公司受低质量审计传染的影响作为“审计风... 详细信息

低质量审计传染对于审计师与上市公司具有负面影响。本文采取2016-2022年我国A股上市公司的数据,基于财务重述事件中错报年时,上市公司之间因相同签字审计师产生的审计师联结,将重述年时被联结公司受低质量审计传染的影响作为“审计风险联结”。本文发现,审计师显著倾向于提升关键审计事项披露以应对审计风险联结;异质性分析发现,财务重述事件受到监管部门披露、期间发生过审计师变更及审计师行业专长均能显著加强上述效应;拓展性研究发现,在审计风险联结情境下,关键审计事项披露能够显著提升审计质量。本文结论有助于认识声誉维护动机下低质量审计传染对于审计师行为的影响,并识别了关键审计事项披露在其中发挥的应对作用与治理效果。

关键词：审计风险联结关键审计事项披露财务重述低质量审计传染