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Unveiling the performance of a novel alkalizing bacterium Enterobacter ***-2 in immobilization of available Cd

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Journal of Environmental Sciences 2024年第3期137卷 245-257页

作者： Yixin Luo min liao Xiongxiong Lu Na Xu Xiaomei Xie Weiming Gao College of Environmental and Resource Science Zhejiang UniversityHangzhou 310058China Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment Hangzhou 310058China National Demonstration Center for Experimental Environmental and Resources Education Zhejiang UniversityHangzhou 310058China

A novel alkalizing strain Enterobacter ***-2 that could resist 400 mg/L Cd was isolated from Cd-contaminated soil,which immobilized 96.05%Cd^(2+)from *** distribution analysis demonstrated that more than half of the Cd^(2+)was converted into extracellular precipitated Cd through mobilization of the alkali-producing mechanism by the strain LYX-2,achieving the high immobilization efficiency of Cd^(2+).Biosorption experiments revealed that strain LYX-2 had superior biosorption capacity of 48.28 mg/g for *** experiments with Brassica rapa *** performed with and without strain *** to control,15.92%bioavailable Cd was converted to non-bioavailable Cd and Cd content in aboveground vegetables was decreased by 37.10%with addition of strain *** Cd was mainly immobilized through extracellular precipitation,cell-surface biosorption and intracellular accumulation of strain LYX-2,which was investigated through Cd distribution,Scanning Electron Microscope and Energy-Dispersive X-ray Spectroscopy(SEM-EDS),Fourier Transform Infrared Spectroscopy(FT-IR),X-ray Photoelectron Spectroscopy(XPS)and Transmission Electron Microscopy(TEM)*** addition,the application of strain LYX-2 significantly promoted the growth of vegetables about *** results indicated that highly Cd-resistant alkalizing strain LYX-2,as a novel microbial passivator,had excellent ability and reuse value to achieve the remediation of Cd-contaminated soil coupled with safe production of vegetables simultaneously.

关键词： Cadmium Enterobacter sp.LYX-2 Cd Immobilization Microbial passivator

Effects of contamination of single and combined cadmium and mercury on the soil microbial community structural diversity and functional diversity

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Chinese Journal Of Geochemistry 2011年第3期30卷 366-374页

作者： XIE Xiaomei liao min MA Aili ZHANG Haijun The Center of Experiment Teaching College of Environmental and Resource Sciences Zhejiang University Hangzhou 310058 China Department of Resources Science College of Environmental and Resources Sciences Zhejiang University Hangzhou 310058 China Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition Hangzhou 310058 China

To assess the effects of single and combined pollution of cadmium (Cd) and mercury (Hg) on soil micro-bial community structural and functional diversities, an incubation experiment was conducted, by employing two soils, namely, the marine sediment silty loam soil and the yellowish-red soil, in which five levels of Cd, Hg and Cd and Hg in combination were added. After being incubated for 56 days, the phospholipid fatty acids (PLFAs) profile and sole carbon source utilization pattern (BIOLOG) of the samples were tested. The results showed that the compo-sition of the microbial communities changed significantly at different levels of metals application. The principal component analyses (PCA) of PLFAs indicated that the structure of the microbial community was also significantly altered with increasing levels of metals, with increasing PLFAs biomarkers for fungi and actinomycetes, and in-creasing ratio of Gram-positive to Gram-negative bacteria. Sole carbon source utilization pattern analysis revealed that single and combined application of Cd and Hg inhibited significantly the functional activity of soil microorgan-isms, the functional diversity indices [Richness (S), Shannon-Wiener indices (H) and Evenness (EH)] were signifi-cantly lower in polluted soils than those in non-polluted soils, which also significantly altered with increasing levels of metals. PCA for the sole carbon source utilization pattern also indicated that the metal contamination could result in a variable soil microbial community. The results revealed that the combination of Cd and Hg had higher toxicity to soil microbial community structural and functional diversities than the individual application of Cd or Hg.

关键词：微生物群落结构镉(Cd) 功能多样性污染土壤重金属污染汞土壤微生物群落多样性指数

Selection of better extractants to indicate Pb bioavailability in lead-contaminated agricultural soils of Zhejiang Province

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Chinese Journal Of Geochemistry 2007年第4期26卷 407-413页

作者： ZENG Lusheng, liao min CHEN Chengli HUANG Changyong Department of Resources Science College of Environmental and Resource Sciences Zhejiang University Hangzhou 310029 China

The accumulation of Pb in the food chain is one of the great concerns as it can cause chronic health problems and the available Pb is easily absorbed by crops. To shed light on Pb bioavailability in lead-contaminated agricultural soils of Zhejiang Province, a series of plant growth experiments were performed in a greenhouse to select more suitable extractants from five commonly used extractants (0.1 M HCl, 1 M NH4OAc, 0.1 M CaCl2, DTPA-TEA and Mehlich 3). The results showed that 1 M NH4OAc can extract Pb best, indicating the Pb bioavailability, then, DTPA-TEA and 0.1 M HCl in the tested soils. In case 1 M NH4OAc was used as an extractant, the critical Pb concentrations in soils were 50.19 mg/kg, 21.16 mg/kg and 114.00 mg/kg (1 M NH4OAc extracted Pb) in silty loam, yellowish-red soil and purplish soil, respectively. When the values exceed the above ones, the contents of Pb in Chinese cabbage leaves will exceed the Chinese Tolerance Limit of Lead in Foods (GB14935-94), as a result, potential ecological risk and hazard to human health via the food chain will appear.

关键词：生物利用率芸苔铅环境污染萃取剂

Combination of Dexmedetomidine and Butorphanol Optimized Sedation in Drug-Induced Sleep Endoscopy: A Randomized, Double-Blind Trial

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Current Medical Science 2021年第6期41卷 1247-1251页

作者： Xin-hao ZHANG Shan LIU Wan-min liao Department of Otorhinolaryngology Head and Neck Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Anesthesiology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Objective:Drug-induced sleep endoscopy(DISE)allows for the evaluation of dynamic airway collapse in patients with obstructive sleep ***,a standardized sedation regimen for DISE is not yet *** study aimed to investigate the safety profiles and efficacies of dexmedetomidine combined with butorphanol for ***:Sixty patients with obstructive sleep apnea scheduled to undergo DISE were randomly divided into Group D and Group *** recipients were initially given intravenous butorphanol(1 mg)(Group DB)or saline(Group D).Subsequently,both groups were sedated using a loading dose of 1.0 pg/kg/h of *** and respiratory parameters,the time to attain sufficient sedation,wakeup time,and adverse events during DISE were ***:Compared with Group D,the time until sufficient sedation and wakeup time in Group DB were significantly reduced.A higher performer satisfaction level was achieved in Group *** in Group DB showed a higher incidence of bradycardia compared with Group ***,the bradycardia resolved spontaneously in both groups without any *** was no instance of cough,hypotension,arrhythmia,nausea or vomiting in either ***:Compared to dexmedetomidine alone,a small dose of butorphanol infusion(1 mg)as an adjunct treatment to dexmedetomidine during DISE can reduce the dosage of dexmedetomidine,shorten the time until sufficient sedation and enhance the performer satisfaction *** synergistic combination could be a promising sedation regimen for DISE in terms of procedural convenience and patient safety.

关键词： drug-induced sleep endoscopy dexmedetomidine butorphanol obstructive sleep apnea

Changes of Soil Microbiological Characteristics After Solidago canadensis L. Invasion

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Agricultural Sciences in China 2011年第7期10卷 1064-1071页

作者： liao min XIE Xiao-mei PENG Ying MA Ai-li Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition/Department of Resources Science College of Environmental and Resources Sciences Zhejiang University Hangzhou 310058 P.R.China Research Center for Eco-Environmental Sciences College of Environmental and Resource Sciences Zhejiang University Hangzhou 310058 P.R.China

The relationship between Solidago canadensis L. invasion and soil microbial communities was studied across the invasive gradients varying from 0 to 40, 80, and 100% coverage of S. canadensis. The results showed both soil microbial biomass C （Cmic） and N （Nmic） increased as the coverage of S. canadensis increased. Soil microbial quotient Cmic/Corg （microbial biomass C/organic C） tended to increase linearly with the coverage of S. canadensis. Soil basal respiration （BR） also showed a similar trend. The soil respiratory quotient qCO2 decreased with S. canadensis invasion, and remained at quite a constantly low level in the invasive soils. Sole carbon source utilization profiles analyses indicated that S. canadensis invasion tended to result in higher microbial functional diversity in the soil. Average utilization of specific substrate guilds was highest in the soil with S. canadensis monoculture. Principle component analysis of sole carbon source utilization profiles further indicated that microbial functional diversity in the soil with S. canadensis monoculture was distinctly separated from those soils in the native area and the ecotones. In conclusion, S. canadensis invasion improved soil microbial biomass, respiration and utilization of carbon sources, and decreased qCO2, thus created better soil conditions, which in turn were more conducive to the growth of S. canadensis.

关键词： sole carbon source utilization functional diversity microbial biomass microbial respiratory Solidago canadensis

A general and efficient Lewis acid catalysed Mukaiyama-aldol reaction of difluoroenoxysilanes and ketones

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Science Bulletin 2017年第22期62卷 1504-1509页

作者： Fu-min liao Xiao-Tong Gao Xiao-Si Hu Shi-Liang Xie Jian Zhou Shanghai Key Laboratory of Green Chemistry and Chemical Processes School of Chemistry and Molecular Engineering East China Normal University Shanghai 200062 China State Key Laboratory of Elemento-organic Chemistry Nankai University Tianjin 300071 China Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development School of Chemistry and Chemical Engineering East China Normal University Shanghai 200062 China

We report a general and highly efficient Mukaiyama-aldol reaction of ketones and *** the reaction of aryl ketones worked efficiently in the presence of Bi(OTf)_3, that of aliphatic ketones required the use of Sc(OTf_)3. In addition, Sc(OTf)_3 was capable of achieving excellent 1,2-selectivity in the corresponding reaction of α,β-unsaturated ketones. This method provides a facile access to differently substituted β-hydroxy α,α-difluoro ketones, versatile synthons for difluomethylated tertiary alcohols.

关键词： Lewis acid catalysed Mukaiyama-aldol reaction Ketones Difluoroenoxysilanes β-Hydroxy α,α-difluoro ketones

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration

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Signal Transduction and Targeted Therapy 2023年第10期8卷 4927-4941页

作者： Jinpeng Du Wenwei liao Haichuan Wang Guimin Hou min liao Lin Xu Jiwei Huang Kefei Yuan Xiangzheng Chen Yong Zeng Division of Liver Surgery Department of General Surgery and Laboratory of Liver Surgeryand State Key Laboratory of Biotherapy and Collaborative Innovation Center of BiotherapyWest China HospitalSichuan UniversityChengduSichuan 610041China Department of Hepatobiliary and Pancreatic Surgery The First Affliated Hospital of Zhengzhou UniversityZhengzhouHenan 450052China Department of Thoracic Surgery The Second Clinical Medical CollegeJinan University(Shenzhen People's Hospital)Shenzhen 518020China The First Affliated Hospital Jinan UniversityGuangzhouGuangdong 510630China Department of Hepato-Biliary-Pancreatic Surgery Sichuan Cancer Hospital&InstituteSichuan Cancer CenterThe Affliated Cancer Hospital of University of Electronic Science and Technology of ChinaChengduSichuan 610041China Department of Medical Ultrasound West China HospitalSichuan UniversityChengduSichuan 610041China

The mineral dust-induced gene(MDIG)comprises a conserved JmjC domain and has the ability to demethylate histone H3 lysine 9 trimethylation(H3K9me3),Previous studies have indicated the significance of MDIG in promoting cell proliferation by modulating cell-cycle ***,its involvement in liver regeneration has not been extensively *** this study,we generated mice with liver-specific knockout of MDIG and applied partial hepatectomy or carbon tetrachloride mouse models to investigate the biological contribution of MDIG in liver regeneration.

关键词： transition involvement liver

HfO_(2)-based ferroelectric thin film and memory device applications in the post-Moore era:A review

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Fundamental Research 2023年第3期3卷 332-345页

作者： Jiajia liao Siwei Dai Ren-Ci Peng Jiangheng Yang Binjian Zeng min liao Yichun Zhou a School of Advanced Materials and Nanotechnology Xidian UniversityXi’an 710126China Frontier Research Center of Thin Films and Coatings for Device Applications Academy of Advanced Interdisciplinary ResearchXidian UniversityXi’an 710126China Key Laboratory of Low Dimensional Materials and Application Technology of ministry of Education School of Materials Science and EngineeringXiangtan UniversityXiangtan 411105China

The rapid development of 5G,big data,and Internet of Things(IoT)technologies is urgently required for novel non-volatile memory devices with low power consumption,fast read/write speed,and high reliability,which are crucial for high-performance *** memory has undergone extensive investigation as a viable alternative for commercial applications since the post-Moore ***,conventional perovskite-structure ferroelectrics(e.g.,PbZr_(x)Ti_(1-x)O_(3))encounter severe limitations for high-density integration owing to the size effect of ferroelectricity and incompatibility with complementary metal-oxide-semiconductor *** 2011,the ferroelectric field has been primarily focused on HfO_(2)-based ferroelectric thin films owing to their exceptional *** reviews discussing the control of ferroelectricity and device applications *** is believed that a comprehensive understanding of mechanisms based on industrial requirements and concerns is necessary,such as the wake-up effect and fatigue *** mechanisms reflect the atomic structures of the materials as well as the device ***,a review focusing on phase stability and domain structure is *** addition,the recent progress in related ferroelectric memory devices and their challenges is briefly discussed.

关键词： Hfo_(2)ferroelectrics Phase stability Domain structure Wake-up Fatigue Ferroelectric field-effect transistor

Domain Wall Evolution in Hf_(0.5)Zr_(0.5)O_(2)Ferroelectrics under Field-Cycling Behavior

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Research 2023年第4期6卷 415-423页

作者： Sirui Zhang Qinghua Zhang Fanqi Meng Ting Lin Binjian Zeng Lin Gu min liao Yichun Zhou School of Advanced Materials and Nanotechnology Xidian UniversityXi’an 710071China Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China School of Materials Science and Engineering Xiangtan UniversityXiangtan 411105China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

HfO_(2)-based ferroelectrics have evoked considerable interest owing to the complementary metal-oxide semiconductor compatibility and robust ferroelectricity down to a few unit ***,the unique wake-up effect of HfO_(2)-based ferroelectric films severely restricts the improvement of their *** particular,the domain structure is an important characteristic of ferroelectric materials,which still has not been well understood in HfO_(2)-based *** this work,a Hf_(0.5)Zr_(0.5)O_(2) ferroelectric thin film is grown on a typical Si substrate buffered with TiN *** 90°domains of the Pca21 ferroelectric phase with head-to-tail and tail-to-tail structures can be observed by Cs-corrected scanning transmission electron microscope under their pristine *** waking up,the 180°domain is displayed in the ferroelectric *** remarkable differences in domain walls for 90°and 180°domains are characterized by qualitatively mapping the polarization distributions at the atomic *** domain wall changes from the[101]of the Hf_(0.5)Zr_(0.5)O_(2) film to the[001]of the Hf_(0.5)Zr_(0.5)O_(2) *** result provides fundamental information for understanding the domain structure of HfO_(2)-based ferroelectrics.

关键词： ferroelectric film walls