Effects of contamination of single and combined cadmium and mercury on the soil microbial community structural diversity and functional diversity

作     者：XIE Xiaomei LIAO Min MA Aili ZHANG Haijun 

作者机构：The Center of Experiment Teaching College of Environmental and Resource Sciences Zhejiang University Hangzhou 310058 China Department of Resources Science College of Environmental and Resources Sciences Zhejiang University Hangzhou 310058 China Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition Hangzhou 310058 China 

出 版 物：《Chinese Journal Of Geochemistry》 (中国地球化学学报)

年 卷 期：2011年第30卷第3期

页      面：366-374页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0709[理学-地质学] 0819[工学-矿业工程] 07[理学] 08[工学] 0708[理学-地球物理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China (Project No. 40201026) the State Key Basic Research and Development Plan of China (No. 2002CB410804) 

主　　题：微生物群落结构 镉(Cd) 功能多样性 污染土壤 重金属污染 汞 土壤微生物群落 多样性指数 

摘      要：To assess the effects of single and combined pollution of cadmium (Cd) and mercury (Hg) on soil micro-bial community structural and functional diversities, an incubation experiment was conducted, by employing two soils, namely, the marine sediment silty loam soil and the yellowish-red soil, in which five levels of Cd, Hg and Cd and Hg in combination were added. After being incubated for 56 days, the phospholipid fatty acids (PLFAs) profile and sole carbon source utilization pattern (BIOLOG) of the samples were tested. The results showed that the compo-sition of the microbial communities changed significantly at different levels of metals application. The principal component analyses (PCA) of PLFAs indicated that the structure of the microbial community was also significantly altered with increasing levels of metals, with increasing PLFAs biomarkers for fungi and actinomycetes, and in-creasing ratio of Gram-positive to Gram-negative bacteria. Sole carbon source utilization pattern analysis revealed that single and combined application of Cd and Hg inhibited significantly the functional activity of soil microorgan-isms, the functional diversity indices [Richness (S), Shannon-Wiener indices (H) and Evenness (EH)] were signifi-cantly lower in polluted soils than those in non-polluted soils, which also significantly altered with increasing levels of metals. PCA for the sole carbon source utilization pattern also indicated that the metal contamination could result in a variable soil microbial community. The results revealed that the combination of Cd and Hg had higher toxicity to soil microbial community structural and functional diversities than the individual application of Cd or Hg.