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Clinical significance of serum intercellular adhesion molecule-i detection in patients with hepatocellular carcinoma

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World Journal of Gastroenterology 2000年第3期6卷 408-410页

作者： Ming Hui Mei Jing Xu qin Fen Shi Jing Hong Yang Qian CHen li ling qin department of hepatobiliary surgery, institute of hepatobiliary surgery,guilin Medical College,guilin 541001,guangxi province,china

INTRODUCTION Since the late 1980s,studies on the expression ofintercellular adhesion molecule-1(ICAM-1)inpatients with malignancies have demonstrated thatICAM-1 may strongly express in two forms in suchdiseases:membra... 详细信息

关键词： carcinoma hepatocellular/diagnosis intercellular adhesion molecule-1 liverneoplasms/diagnosis alpha-fetoprotein

Experimental research on TECA-I bioartificial liver support system to treat canines with acute liver failure

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World Journal of Gastroenterology 2001年第5期7卷 706-709页

作者： Xiao-Ping Chen~2 Yi-Long Xue~1 Xin-Jian li~1 Zuo-Yun Zhang~1 Yan-ling li~1 Zhi-Qiang Huang~2 1 institute of Basic Medical Sciences,2 department of hepatobiliary surgery,PLA General Hospital,Beijing 100853,china institute of Basic Medical Sciences PLA General Hospital Beijing 100853 China.

AIM: To evaluate the efficacy and safety of the TECA-I bioartificial liver support system (BALSS) in treating canines with acute liver failure (ALF). METHODS: Ten canines with ALF induced by 80% liver resection received BALSS treatment (BALSS group). Blood was perfused through a hollow fiber tube containing 1X10(10) porcine *** canines with ALF were treated with BALSS without porcine hepatocytes (control group), and five canines with ALF received drug treatment (drug group). Each treatment lasted 6 hours. RESULTS: BALSS treatment yielded beneficial effects for partial liver resection induced ALF canines with survival and decreased plasma ammonia, ALT, AST and BIL. There was an obvious decrease in PT level and increase in PA level, and there were no changes in the count of lymphocytes, immunoglobulins (IgA, IgG and IgM) and complement (C3 and C4) levels after BALSS treatment. In contrast, for the canines with ALF in non-hepatocyte BALSS group (control group) and drug group, there were no significant changes in ammonia, ALT, AST, BIL, PT and PA levels. ALF canines in BALSS group, control group and drug group lived respectively an average time of 108.0h +/- 12.0h, 24.0h +/- 6.0h and 20.4h +/- 6.4h,and three canines with ALF survived in BALSS group. CONCLUSION: TECA-I BALSS is efficacious and safe for ALF canines induced by partial liver resection.

关键词： liver, Artificial Animals Blood Coagulation Dogs Hepatectomy Hepatocytes Kidney liver liver Failure, Acute Lung Myocardium Swine

Apolipoprotein E4 interferes with lipid metabolism to exacerbate depression-like behaviors in 5xFAD mice

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Animal Models and Experimental Medicine 2024年第3期7卷 347-361页

作者： Yanju Gong Mingfeng li Min liu Xinghan Wu Yanhong li Chuan qin ling Zhang iNHC Key Laboratory of Human Disease Comparative Medicine Beijing Engineering Research Center for Experimental Animal Models of Human Critical DiseasesInternational Center for Technology and lnnovation of Animal ModelComparative Medicine CenterInstitute of Laboratory Animal SciencesChinese Academy of Medical Sciences(CAMS)and Peking Union Medical College(PuMC)BeijingChina Changping National Laboratory(CPNL) BeijingChina

Background:Apolipoprotein E4(ApoE4)allele is the strongest genetic risk factor for late-onset Alzheimer's disease,and it can aggravate depressive symptoms in non-AD ***,the impact of ApoE4 on AD-associated depression-l ike behaviors and its underlying pathogenic mechanisms remain ***:This study developed a 5xFAD mouse model overexpressing human ApoE4(E4FAD).Behavioral assessments and synaptic function tests were conducted to explore the effects of ApoE4 on cognition and depression in 5xFAD *** in peripheral and central lipid metabolism,as well as the levels of serotonin(5-HT)andγ-aminobutyric acid(GABA)neurotransmitters in the prefrontal cortex,were *** addition,the protein levels of 24-dehydrocholesterol reductase/glycogen synthase kinase-3 beta/mammalian target of rapamycin(DHCR24/GSK3β/m TOR)and postsynaptic density protein 95/calmodulin-dependent protein kinase II/brain-derived neurotrophic factor(PSD95/CaMK-II/BDNF)were measured to investigate the molecular mechanism underlying the effects of ApoE4 on AD ***:Compared with 5xFAD mice,E4FAD mice exhibited more severe depressionlike behaviors and cognitive *** mice also exhibited increased amyloid-beta deposition in the hippocampus,increased astrocyte numbers,and decreased expression of depression-related neurotransmitters 5-HT and GABA in the prefrontal ***,lipid metabolism disorders were observed in E4FAD,manifesting as elevated low-density lipoprotein cholesterol and reduced high-density lipoprotein cholesterol in peripheral blood,decreased cholesterol level in the prefrontal cortex,and reduced expression of key enzymes and proteins related to cholesterol synthesis and *** expression of proteins related to the DHCR24/GSK3β/m TOR and PSD95/CaMK-II/BDNF pathways was also ***:This study found that ApoE4 overexpression exacerbates depressionlike behaviors in 5xFAD mice and confirmed that ApoE4 reduces co

关键词： 5xFAD mice Alzheimer's disease ApoE4 allele depression like

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Toll-like receptor 4-mediated apoptosis of pancreatic cells in cerulein-induced acute pancreatitis in mice

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hepatobiliary & Pancreatic Diseases International 2010年第6期9卷 645-650页

作者： Si-qin Ding,Yuan li,Zong-Guang Zhou,Cun Wang,Lan Zhan and Bin Zhou institute of Digestive surgery,department of Gastrointestinal surgery,and department of Pediatric surgery, West china Hospital,Sichuan University,Chengdu 610041,china institute of Digestive surgery West China Hospital Sichuan University Chengdu 610041 China.

BACKGROUND:Toll-like receptor 4(TLR4)plays an important role in the occurrence and development of acute pancreatitis (AP).Apoptosis of pancreatic cells is closely related to the severity of ***4 is known to induce apoptosis in some cell types and therefore it is of importance to investigate potential associations between TLR4 activity and apoptosis in the setting of AP. METHODS:A total of 50 wild-type(C57BL/10J)and TLR4-deficient(C57BL/10ScNJ)mice were divided into three groups:2-hour,4-hour,and control *** group was divided into two equal subgroups:TLR4-wild-type mice and TLR4-deficient *** was experimentally induced by 7 intraperitoneal injections of 50μg/kg cerulein at hourly *** mice received 7 injections of equal volumes of *** severity of pancreatic injury during AP was assessed by serum amylase concentration and histopathology. The level of apoptosis of pancreatic cells in response to AP was evaluated by calculating the apoptotic index(AI)and comparing the expression levels of cytochrome C and Fasassociated protein with death domain(FADD)between TLR4-wild-type and TLR4-deficient mice at 2 time points. RESULTS:The AI was found to be significantly lower in the pancreas of TLR4-deficient mice with AP compared to TLR4-wild-type mice at two hours after the last treatment ***-linked immunosorbent assay and real-time reverse transcription-polymerase chain reaction also revealed significantly lower expression of cytochrome C and FADD in the pancreas of TLR4-deficient mice than in TLR4-wild-type animals at the same time *** amylase concentration and morphological severity of AP in pancreatic tissue were found to be similar in the two strains of mice at both time ***:We postulate that TLR4 can mediate apoptosis of pancreatic cells during the early stages of AP,via the activation of both intrinsic and extrinsic apoptotic signaling pathways.

关键词： acute pancreatitis toll-like receptor 4 apoptosis cytochrome C Fas-associated protein with death domain

Greater stress protein expression enhanced by combined prostaglandin A1 and lithium in a rat model of focal ischemia

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Acta Pharmacologica Sinica 2007年第8期28卷 1097-1104页

作者： Xi-hui XU~(2,3) Yi-nan HUA~(2,3) Hui-ling ZHANG~(2,3) Jun-chao WU~(2,3) You-zhu MIAO~(2,3) Rong HAN~(2,3) Zhen-lun GU~(2,3) Zheng-hong qin~(2,3,4) ~2Laboratory of Aging and Nervous Disease and ~3department of Pharmacology,Soochow University School of Medicine Suzhou 215123,china Laboratory of Aging and Nervous Disease Soochow University School of Medicine Suzhou China department of Pharmacology Soochow University School of Medicine Suzhou China

Aim:To investigate the effects of lithium(li)and prostaglandin A1(PGA1)on theexpression of heat shock factor 1(HSF- 1),heat shock proteins(HSP),and apoptosisprotease activating factor-1(Apaf-1)induced by permanent focal ischernia in ***:The rats were pretreated with a subcutaneous(sc)injection of li for 2 dor a single intracerebral ventricle(icv)administration of PGA1for 15 min beforeischemic insult,or a combination ofli(sc,1 mEq/kg,2 d)and PGA1(icv,15 minprior to ischemic insult).Brain ischemia was induced by the permanent middlecerebral artery occlusion(pMCAO).Twenty-four hours after the occlusion,theexpression of HSF-1,HSE and Apaf-1 in the ischemic striatum were examined withWestern blot ***:The expression of HSF-1,heme oxygenase-1(HO-1),HSP90α,and Apaf-1 were significantly increased,but the expression ofHSP90β was significantly decreased 24 h after the ***1and li and theircombination significantly enhanced the ischemia-induced elevation in the levelsof HSF-1,HO-1,and HSP90α,and recovered HSP90β expression,but decreasedApaf-1 levels in the ischemic ***:The present study demon-strates that PGA1and li have synergistic effects on the enhancement of theexpression of HSP,suggesting that the synergistic effects of PGA1and li in therat model of permanent focal cerebral ischemia may be mediated by the enhance-ment expression of HSP expression and the downregulation of *** studiessuggest that combined PGA1and li may have potential clinical value for thetreatment of stroke.

关键词： prostaglandin A1 lithium oxidative stress heat shock factor 1 cerebral ischemia Apaf-IHO-1 HSP90α HSP90β

Green tea polyphenol epigallocatechin-3-gallate suppresses rat hepatic stellate cell invasion by inhibition of MMP-2 expression and its activation

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Acta Pharmacologica Sinica 2006年第12期27卷 1600-1607页

作者： Mao-chuan ZHEN Xiao-hui HUANG Qian WANG~2 Kai SUN Yun-jian liU Wen li Long-juan ZHANG liang-qi CAO Xi-ling CHEN department of hepatobiliary surgery,the First Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510080,china department of hepatobiliary surgery the First Affiliated Hospital of Sun Yat-Sen University Guangzhou China

Aim:Epigallocatechin-3-gallate(EGCG)is the major component of green teapolyphenols,whose wide range of biological properties includes *** metalloproteinases(MMP)that participate in extracellular matrixdegradation are involved in the development of hepatic *** presentstudy investigates whether EGCG inhibits activation of the major gelatinase matrixmetalloproteinase-2(MMP-2)in rat hepatic stellate cells(HSC).Methods:Theexpression of MMP-2,tissue inhibitors of metalloproteinases-2(TIMP-2),andmembrane-type 1-MMP(MT1-MMP)was assessed by RT-PCR and Western ***-2 activity was evaluated by zymography and MT1-MMP activitywas assessed by an enzymatic *** migration was measured by a woundhealing assay and cell invasion was performed using Transwell cell ***:The expression of MMP-2 mRNA and protein in HSC wassubstantially reduced by EGCG *** treatment also reduced con-canavalin A(ConA)-induced activation of secreted MMP-2 and reduced MT1-MMP activity in a dose-dependent *** addition,EGCG inhibited eitherHSC migration or ***:The abilities of EGCG to suppress MMP-2 activation and HSC invasiveness suggest that EGCG may be useful in the treat-ment and prevention of hepatic fibrosis.

关键词： matrix metalloproteinase hepatic stellate cell green tea migration invasion

Automatic detection of small bowel lesions with different bleeding risks based on deep learning models

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World Journal of Gastroenterology 2024年第2期30卷 170-183页

作者： Rui-Ya Zhang Peng-Peng Qiang ling-Jun Cai Tao li Yan qin Yu Zhang Yi-qing Zhao Jun-Ping Wang department of Gastroenterology The Fifth Clinical Medical College of Shanxi Medical UniversityTaiyuan 030012Shanxi ProvinceChina School of Computer and Information Technology Shanxi UniversityTaiyuan 030006Shanxi ProvinceChina School of life Sciences and Technology Mudanjiang Normal UniversityMudanjiang 157011Heilongjiang ProvinceChina

BACKGROUND Deep learning provides an efficient automatic image recognition method for small bowel(SB)capsule endoscopy(CE)that can assist physicians in ***,the existing deep learning models present some unresolved *** To propose a novel and effective classification and detection model to automatically identify various SB lesions and their bleeding risks,and label the lesions accurately so as to enhance the diagnostic efficiency of physicians and the ability to identify high-risk bleeding *** The proposed model represents a two-stage method that combined image classification with object ***,we utilized the improved ResNet-50 classification model to classify endoscopic images into SB lesion images,normal SB mucosa images,and invalid ***,the improved YOLO-V5 detection model was utilized to detect the type of lesion and its risk of bleeding,and the location of the lesion was *** constructed training and testing sets and compared model-assisted reading with physician *** The accuracy of the model constructed in this study reached 98.96%,which was higher than the accuracy of other systems using only a single *** sensitivity,specificity,and accuracy of the model-assisted reading detection of all images were 99.17%,99.92%,and 99.86%,which were significantly higher than those of the endoscopists’*** image processing time of the model was 48 ms/image,and the image processing time of the physicians was 0.40±0.24 s/image(P1).CONCLUSION The deep learning model of image classification combined with object detection exhibits a satisfactory diagnostic effect on a variety of SB lesions and their bleeding risks in CE images,which enhances the diagnostic efficiency of physicians and improves the ability of physicians to identify high-risk bleeding groups.

关键词： Artificial intelligence Deep learning Capsule endoscopy Image classification Object detection Bleeding risk

Changes in the Non-targeted Metabolomic Profile of Three-year-old Toddlers with Elevated Exposure to Polycyclic Aromatic Hydrocarbons

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Biomedical and Environmental Sciences 2024年第5期37卷 479-493页

作者： li Yang liN Dan ZHANG Xiu qin JU Guang Xiu SU Ya ZHANG Qian DUAN Hai Ping YU Wei Sen WANG Bing ling PANG Shu Tao department of Epidemiology and Health Statistics School of Public HealthQingdao UniversityQingdao 266021ShandongChina department of Environmental Health Qingdao Municipal Center for Disease Control and PreventionQingdao Institute of Preventive MedicineQingdao 266033ShandongChina Physicochemical Laboratory Qingdao Municipal Center for Disease Control and PreventionQingdao Institute of Preventive MedicineQingdao 266033ShandongChina Section of Child Health Care Huangdao Maternity and Child Health Care Hospital of QingdaoQingdao 266499ShandongChina qingdao Blood Center Qingdao 266071ShandongChina

Objective To investigate changes in the urinary metabolite profiles of children exposed to polycyclic aromatic hydrocarbons(PAHs)during critical brain development and explore their potential link with the intestinal *** liquid chromatography-tandem mass spectrometry was used to determine ten hydroxyl metabolites of PAHs(OH-PAHs)in 36-month-old ***,37 children were categorized into low-and high-exposure groups based on the sum of the ten ***-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to identify non-targeted metabolites in the urine ***,fecal flora abundance was assessed by 16S rRNA gene sequencing using Illumina *** The concentrations of 21 metabolites were significantly higher in the high exposure group than in the low exposure group(variable importance for projection>1,Plites were positively correlated with the hydroxyl metabolites of naphthalene,fluorine,and phenanthrene(r=0.336–0.531).The identified differential metabolites primarily belonged to pathways associated with inflammation or proinflammatory states,including amino acid,lipid,and nucleotide ***,these distinct metabolites were significantly associated with specific intestinal flora abundances(r=0.34–0.55),which were mainly involved in *** Higher PAH exposure in young children affected metabolic homeostasis,particularly that of certain gut microbiota-derived *** investigation is needed to explore the potential influence of PAHs on the gut microbiota and their possible association with neurodevelopmental outcomes.

关键词： Child Gut microbiota Non-targeted metabolomics Polycyclic aromatic hydrocarbons Urinary metabolite profile

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Pien Tze Huang Inhibits Proliferation of Colorectal Cancer Cells through Suppressing PNO1 Expression and Activating p53/p21 Signaling Pathway

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Chinese Journal of Integrative Medicine 2024年第6期30卷 515-524页

作者： CAO liu-jing liU li-ya CHEN You-qin HAN Yu-ying WEI li-hui YAO Meng-ying FANG Yi WU Mei-zhu CHENG Ying Thomas J.Sferra liU Hui-xin li li PENG Jun SHEN A-ling Clinical Research institute the Second Affiliated Hospital&Academy of Integrative MedicineFujian University of Traditional Chinese MedicineFuzhou350122China Fujian Key Laboratory of Integrative Medicine in Geriatrics Fujian University of Traditional Chinese MedicineFuzhou350122China department of Pediatrics Case Western Reserve University School of MedicineUH Rainbow Babies and Children’s HospitalClevelandOH44106USA Innovation and Transformation Center Fujian University of Traditional Chinese MedicineFuzhou350122China department of Health Management Fujian Provincial HospitalShengli Clinical College of Fujian Medical UniversityFuzhou000000China

ObjectiveTo explore the regulatory effect of Pien Tze Huang (PZH) on targeting partner of NOB1 (PNO1) and it’s down-stream mediators in colorectal cancer (CRC) *** polymerase chain reaction was performed to determine mRNA levels of PNO1, TP53, and CDKN1A. Western blotting was performed to determine protein levels of PNO1, p53, and p21. HCT-8 cells were transduced with a lentivirus over-expressing PNO1. Colony formation assay was used to detect cell survival in PNO1 overexpression of HCT-8 cells after PZH treatment. Cell-cycle distribution, cell viability and cell apoptosis were performed to identify the effect of PNO1 overexpression on cell proliferation and apoptosis of HCT-8 cells after PZH treatment. Xenograft BALB/c nude mice bearing HCT116 cells transduced with sh-PNO1 or sh-Ctrl lentivirus were evaluated. Western blot assay was performed to detect PNO1, p53, p21 and PCNA expression in tumor sections. Terminal deoxynucleotidyl transferase dUTP nick end labling (TUNEL) assay was used to determine the apoptotic cells in *** treatment decreased cell viability, down-regulated PNO1 expression, and up-regulated p53 and p21 expressions in HCT-8 cells (P1 overexpression attenuated the effects of PZH treatment, including the expression of p53 and p21, cell growth, cell viability, cell cycle arrest and cell apoptosis in vitro (P1 knockdown eliminated the effects of PZH treatment on tumor growth, inhibiting cell proliferation inhibition and apoptosis induction in vivo (P1 knockdown attenuated the effects of PZH treatment on the down-regulation of PNO1 and up-regulation of p53 and p21 in vivo (Pliferative effect is at least in part by regulating the expression of PNO1 and its downstream targets p53 and p21.

关键词： colorectal cancer Pien Tze Huang Chinese medicine partner of NOB1 proliferation p53/p21 pathway

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