Greater stress protein expression enhanced by combined prostaglandin A1 and lithium in a rat model of focal ischemia
Greater stress protein expression enhanced by combined prostaglandin A1 and lithium in a rat model of focal ischemia作者机构：Laboratory of Aging and Nervous Disease Soochow University School of Medicine Suzhou China Department of Pharmacology Soochow University School of Medicine Suzhou China
出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))
年 卷 期：2007年第28卷第8期
页 面：1097-1104页
核心收录：
学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学]
基 金：Project supported by grants from the Department of Education of Jiangsu Province(No 03KJB310123) the Natural Science Foundation of Jiangsu Province(No BK2004037) the National Natural Science Foundation of China(No 30470587)
主 题：prostaglandin A1 lithium oxidative stress heat shock factor 1 cerebral ischemia Apaf-IHO-1 HSP90α HSP90β
摘 要：Aim:To investigate the effects of lithium(Li)and prostaglandin A1(PGA1)on theexpression of heat shock factor 1(HSF- 1),heat shock proteins(HSP),and apoptosisprotease activating factor-1(Apaf-1)induced by permanent focal ischernia in ***:The rats were pretreated with a subcutaneous(sc)injection of Li for 2 dor a single intracerebral ventricle(icv)administration of PGA1for 15 min beforeischemic insult,or a combination ofLi(sc,1 mEq/kg,2 d)and PGA1(icv,15 minprior to ischemic insult).Brain ischemia was induced by the permanent middlecerebral artery occlusion(pMCAO).Twenty-four hours after the occlusion,theexpression of HSF-1,HSE and Apaf-1 in the ischemic striatum were examined withWestern blot ***:The expression of HSF-1,heme oxygenase-1(HO-1),HSP90α,and Apaf-1 were significantly increased,but the expression ofHSP90β was significantly decreased 24 h after the ***1and Li and theircombination significantly enhanced the ischemia-induced elevation in the levelsof HSF-1,HO-1,and HSP90α,and recovered HSP90β expression,but decreasedApaf-1 levels in the ischemic ***:The present study demon-strates that PGA1and Li have synergistic effects on the enhancement of theexpression of HSP,suggesting that the synergistic effects of PGA1and Li in therat model of permanent focal cerebral ischemia may be mediated by the enhance-ment expression of HSP expression and the downregulation of *** studiessuggest that combined PGA1and Li may have potential clinical value for thetreatment of stroke.
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