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Effects of residual organics in municipal wastewater on hydrogenotrophic denitrifying microbial communities

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Journal of Environmental Sciences 2018年第3期30卷 262-270页

作者： Wei Xing Jinlong li Peng li Chong Wang Yanan Cao desheng li Yunfeng Yang Jizhong Zhou Jiane Zuo School of Civil Engineering Beijing Jiaotong University Beijing 100044 China State Key Joint Laboratory of Environmental Simulation and Pollution Control School of Environment Tsinghua University Beijing 100084 China Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard Beijing 100044 China College of Science Flunan Agricultural University Changsha 410128 China Institute for Environmental Genomics and Department of Microbiology and Plant Biology University of Oklahoma Norman OK 73019 USA Earth Sciences Division Lawrence Berkeley National Laboratory Berkeley CA 94720 USA

Hydrogenotrophic denitrification is promising for tertiary nitrogen removal from municipal wastewater. To reveal the influence of residual organics in municipal wastewater on hydrogenotrophic denitrifiers, we adopted high-throughput 16 S r RNA gene amplicon sequencing to examine microbial communities in hydrogenotrophic denitrification enrichments. Using effluent from a municipal wastewater treatment plant as water source, COD,nitrate and p H were controlled the same except for a gradient of biodegradable carbon（i.e., primary effluent（PE）, secondary effluent（SE）, or combined primary and secondary effluent（CE））. Inorganic synthetic water（IW） was used as a control. Hydrogenophaga, a major facultative autotroph, accounted for 17.1%, 5.3%, 32.7% and 12.9% of the sequences in PE, CE,SE and IW, respectively, implicating that Hydrogenophaga grew well with or without ***, which contains likely obligate autotrophic denitrifiers, appeared to be the most dominant genera（23.6%） in IW and accounted for 2.5%, 4.6% and 8.9% in PE, CE and SE,respectively. Thermomonas, which is related to heterotrophic denitrification, accounted for 4.2% and 7.9% in PE and CE fed with a higher content of labile organics, *** contrast, Thermomonas was not detected in IW and accounted for only 0.6% in SE. Our results suggest that Thermomonas are more competitive than Thauera in hydrogenotrophic denitrification with biodegradable organics. Moreover, facultative autotrophic denitrifiers,Hydrogenophaga, are accommodating to residual organic in effluent wastewater, thus we propose that hydrogenotrophic denitrification is amenable for tertiary nitrogen removal.

关键词： Tertiary nitrogen removal Hydrogenotrophic denitrification Residual organics High-throughput sequencing Hydrogenophaga

鄂尔多斯盆地延长组等时格架下的深水坡折带特征及其对砂体的控制作用

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天然气地球科学 2024年第10期35卷 1764-1776页

作者：陈波刘长春李杰王义俊杨雯婷雷启鸿户彬李德胜冷先刚赵中平中国石油长庆油田分公司陇东油气开发分公司甘肃庆阳745000 西安珠峰油气科技有限公司陕西西安710021

鄂尔多斯盆地三叠系延长组依据传统似等厚岩性地层划分方案识别出的沉积坡折带有待商榷。采用井震结合、标志层控制、逐井反复对比等综合方法,通过对多个典型标志层的识别和追踪对比,建立了鄂尔多斯盆地西南部环县地区延长组长7段—长4+... 详细信息

鄂尔多斯盆地三叠系延长组依据传统似等厚岩性地层划分方案识别出的沉积坡折带有待商榷。采用井震结合、标志层控制、逐井反复对比等综合方法,通过对多个典型标志层的识别和追踪对比,建立了鄂尔多斯盆地西南部环县地区延长组长7段—长4+5段部分地层的等时地层格架,并在此基础上识别出了具有典型地貌学特征的深水沉积坡折带。结果表明,环县地区延长组长7段—长4+5段实际由从西南往东北呈多期逐渐减薄的“S”型楔状前积层构成,其内部各套等时地层发育一系列坡折明显且逐渐往东北迁移的深水沉积坡折带,这些坡折带相对于长7段底界最大倾角可达1.9°、宽约6~8.6 km;各期重力流砂体和致密油层主要分布在坡折带处或坡折带前端的斜坡坡脚处,且平行于坡折带呈带状展布,坡折带控砂和控藏作用明显。研究对其他地区延长组深水沉积坡折带的有效识别和油气勘探开发具有重要的借鉴意义。

关键词：鄂尔多斯盆地延长组等时地层格架坡折带砂体

基于UPLC-MS/MS方法分析哈萨克族食管鳞癌患者的血清脂质组学特征

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癌变．畸变．突变 2024年第1期36卷 21-28,34页

作者：刘瑞雪李德生张力为新疆医科大学第一附属医院胸外科新疆乌鲁木齐830054

目的:基于血清脂质组学研究新疆哈萨克族食管鳞状细胞癌(ESCC)患者的脂质代谢特征。方法:选取2018年1月—2020年12月新疆医科大学第一附属医院胸外科就诊的30例哈萨克族ESCC患者(ESCC组),另外选择同期在新疆医科大学健康管理中心体检的... 详细信息

目的:基于血清脂质组学研究新疆哈萨克族食管鳞状细胞癌(ESCC)患者的脂质代谢特征。方法:选取2018年1月—2020年12月新疆医科大学第一附属医院胸外科就诊的30例哈萨克族ESCC患者(ESCC组),另外选择同期在新疆医科大学健康管理中心体检的哈萨克族健康体检者30例(对照组)。采用超高效液相色谱质谱联用技术(UPLC-MS/MS)对两组人群血清进行靶向定量脂质组学分析。根据同类脂质内标峰面积和实际浓度关系进行脂质定量。利用脂质数据进行多变量统计分析。结果:60例血清样本中共筛选出13种脂类,其中浓度最高的是三酰甘油、磷脂酰胆碱和磷脂酰乙醇胺,溶血磷脂酰胆碱是两组人群血清中差异最大的脂类。相较对照组血清样本,哈萨克族ESCC患者血清中三酰甘油、溶血磷脂酰胆碱的浓度上调,磷脂酰乙醇胺下调。溶血磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰胆碱的链长度和不饱和度在哈萨克族ESCC患者血清中显著下调。根据正交偏最小二乘法-判别分析(OPLS-DA),以阈值(P2或<0.5)筛选差异脂质代谢物,共筛选出77种差异脂类,其中与对照组比较,ESCC组上调的有34种,下调的有43种。包括甘油三酯、甘油二酯、溶血磷脂酰胆碱、磷脂酰乙醇胺及游离脂肪酸。结论:哈萨克族ESCC患者血清中不同脂质浓度及变化趋势存在差异。其中甘油三酯、溶血磷脂酰胆碱的浓度上调,磷脂酰乙醇胺、溶血磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰胆碱链长度及不饱和度下调。分析这些脂质分布的变化和筛选新靶标有助于阐明新疆哈萨克族ESCC的发生发展机制。

关键词：食管鳞状细胞癌哈萨克族脂质组学脂质代谢物差异表达

某重油催化裂化装置低负荷工况油浆回炼影响分析

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炼油技术与工程 2024年第10期54卷 22-25页

作者：齐恒山李德生谢铮李林生柳召永大连西太平洋石油化工有限公司辽宁省大连市116600 中国石油石油化工研究院兰州化工研究中心甘肃省兰州市730060

某炼油厂2.80 Mt/a重油催化裂化装置在低负荷情况下进行了为期约6个月的油浆回炼,回炼方式是将循环油浆在原料自保组立处打入混合原料油中,再进入原料油喷嘴。标定结果表明:焦炭收率由7.86%升至8.41%,占比约20%的回炼油浆生成了焦炭;装... 详细信息

某炼油厂2.80 Mt/a重油催化裂化装置在低负荷情况下进行了为期约6个月的油浆回炼,回炼方式是将循环油浆在原料自保组立处打入混合原料油中,再进入原料油喷嘴。标定结果表明:焦炭收率由7.86%升至8.41%,占比约20%的回炼油浆生成了焦炭;装置柴油收率提高,占比约50%的回炼油浆转化成高附加值产品柴油;油浆回炼导致装置总转化率下降,干气、液化石油气、汽油收率共降低1.93百分点。油浆回炼过程中,对原料油及反应系统进行了监控,未发现其对反应系统结焦、烟气轮机运行、原料油管线及原料喷嘴磨损等有明显影响,后续消缺过程中对反应-再生系统内部检查也未发现异常。总体来看,该重油催化裂化装置进行油浆回炼可以提高装置焦炭收率,保证低负荷下全厂蒸汽平衡,可显著降低油浆固含量,有助于降低装置油浆收率。

关键词：重油催化裂化装置低负荷油浆回炼焦炭柴油油浆固含量烟气粉尘浓度

Investigation on Thermal Insulation and Mechanical Strength of lightweight Aggregate Concrete and Porous Mortar in Cold Regions

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Journal of Renewable Materials 2022年第12期10卷 3167-3183页

作者： Jianan Wu Ke Xue Zhaowei Ding Lei Lang Kang Gu Xiaolin li Mingli Zhang desheng li Ningbo City College of Vocational Technology NingboChina College of Water Conservancy and Hydropower Engineering Sichuan Agricultural UniversityYa’anChina State Key Laboratory of Ocean Engineering Shanghai Jiao Tong UniversityShanghaiChina College of Civil Engineering and Architecture Jiaxing UniversityJiaxingChina Department of Civil Engineering Lanzhou University of TechnologyLanzhouChina Department of Civil Engineering College of ScienceNanjing University of Science and TechnologyNanjingChina

Thermal insulation is an important indicator to evaluate the construction material in cold region *** we know,adding the industrial waste as lightweight aggregate or creating the pore inside the cement-based composite could make the texture loose,and the thermal insulating capacity of the material would be improved with this *** these methods,the industrial by-product and engineering waste could be cycled in an efficient ***,after service the fragmented cement composites paste could be used as aggregate in the thermal insulating concrete *** the porous texture is not favorable for the mechanical strength and long-term durability in a cold *** balance the above three requirements from two opposite directions,different processing methods were applied to create the thermal insulation concrete/***,the organic/inorganic lightweight aggregate,including the Expanded Polystyrene(EPS),Expanded Perlite(EP),and Ceramsite(CRMST)particles,were applied to create the lightweight Aggregate Concrete(LWAC).As the comparative tests,the expanded Superabsorbent Polymer(SAP)hydrogel and Air-Entraining Agent(AEA)were also introduced to create the porous *** above concrete/mortar was tested in the normal state and under the Freeze-Thaw cycle to explore the engineering performance in cold *** the experimenting process,the thermal insulation,mechanical strength,and frost resistance of these cement-based composites were investigated,and an optimal thermal insulation concrete/mortar was determined.

关键词： Thermal insulation mechanical strength cement-based composite freeze-thaw cycle frost resistance

Mobility matrix of a weakly coupled parallel multi-DIM isolator based on axial force solution

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High Technology Letters 2020年第3期26卷 335-341页

作者： li desheng Yu Jiangtao Huang Baowang li Zhanxian Wang Zhijun College of Mechanical Engineering North China University of Science and TechnologyTangshan 063210P.R.China

A mobility matrix modeling strategy based on axial force solution for a weakly coupled parallel multi-dimentional(multi-DIM)isolator is *** power flow and transmissibility through the isolator are derived from the mob... 详细信息

关键词： mobility matrix weak-coupling parallel multi-dimentional(multi-DIM)isolator axial force mobility power flow

A fast and effective method to perform paternity testing for Wolong giant pandas

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Chinese Science Bulletin 2011年第24期56卷 2559-2564页

作者： li desheng CUI HengMin WANG ChengDong liNG ShanShan HUANG Zhi ZHANG HeMin China Research and Conservation Center for the Giant Panda Ya'an 625000 China Medical College of Animal Sichuan Agricultural University Ya'an 625000 China Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and State Conservation Center for Gene Resources of Endangered Wildlife Hangzhou 310058 China

Captive populations provide a precious genetic resource for endangered animals and a source of individuals for reintroduction to depleted habitats. Therefore, accuracy in determining paternity is of vital importance for managing captive populations and in selecting representative individuals of known genetic characteristics for release. In this study, we established a fast and effective method to conduct paternity testing for captive giant pandas in the Wolong population. This technique uses two highly polymor phic microsatellites initially, subsequent use of five less polymorphic markers and then paternity exclusion testing carried out using the giant panda paternity exclusion program we have developed. Our results revealed that (1) both sets of markers success- fully identified the real fathers in 25 cases of paternity testing and (2) the success rate of paternity exclusion varied with the degree of polymorphism of the markers used. Subsequently, we conducted correlation analysis between the success rates of paterni- ty identification with these markers, parameters of genetic diversity and tests of neutrality. We found that the paternity exclusion power of microsatellites was significantly correlated with the number of alleles (Na), expected heterozygosity (HE) and observed homozygosity statistic (FO) (all P lites (P = 0.001). Moreover, the first two highly polymorphic loci gave a 100% success rate of excluding non-paternal males because they yielded higher values of Na?HE/FO than the other five less pol- ymorphic markers. Thus, the Na?HE/FO parameter appears suitable to serve as a criterion for selecting microsatellite markers, which could be used for high-resolution molecular techniques of paternity determination among a range of captive animals besides giant pandas.

关键词：圈养大熊猫亲子鉴定卧龙多态性位点分子生物学技术多态标记圈养种群遗传资源

原发性手汗症交感神经切断术后严重代偿性多汗的临床预测模型构建

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中华实验外科杂志 2024年第9期41卷 2116-2119页

作者：马龙孙清超李德生张力为新疆医科大学第一附属医院胸外科乌鲁木齐830054 省部共建中亚高发病成因与防治国家重点实验室乌鲁木齐830054

目的原发性手汗症(PPH)胸腔镜下胸交感神经切断术(ETS)后严重的代偿性多汗(SCH)的临床预测模型构建.方法选取2017年2月至2023年3月在新疆医科大学第一附属医院胸外科行ETS的115例PPH患者资料,并随访收集患者术后SCH的发生情况.根据是否... 详细信息

目的原发性手汗症(PPH)胸腔镜下胸交感神经切断术(ETS)后严重的代偿性多汗(SCH)的临床预测模型构建.方法选取2017年2月至2023年3月在新疆医科大学第一附属医院胸外科行ETS的115例PPH患者资料,并随访收集患者术后SCH的发生情况.根据是否发生术后SCH,将患者分为非SCH(NSCH)组的患者89例,和SCH的患者26例比较两组患者的临床资料.进行单因素和多因素Logistic回归分析判定出发生术后SCH独立危险因素,并构建出预测模型,采用ROC曲线评估预测模型,组间比较采用t检验.结果多因素Logistic回归分析显示,年龄[比值比(OR)=1.111,95%CI:1.026~1.214,P<0.05]、吸烟史(OR=20.345,95%CI:5.623~29.768,P<0.05)和手汗症症状严重程度(OR=4.093,95%CI:1.308~14.702,P<0.05)是发生术后SCH独立危险因素,将这些独立危险因素纳入构建预测模型的ROC曲线下面积为0.874,灵敏度为0.885,特异度为0.697.结论进行ETS术的患者年龄大、有吸烟史以及手汗症症状严重者更容易出现SCH,利用临床预测模型可以识别ETS术后SCH发生的高危人群.

关键词：原发性手汗症胸腔镜下胸交感神经切断术代偿性多汗预测模型

Carrying capacity for the electromagnetic suspension low-speed maglev train on the horizontal curve

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Science China(Technological Sciences) 2010年第4期53卷 1082-1087页

作者： CUI Peng,li Jie & liU desheng Maglev Engineering Center,National University of Defense Technology,Changsha 410073,China Maglev Engineering Center National University of Defense Technology Changsha China

Aiming at the lateral dislocation between the electromagnets and the rails on a horizontal curve,we investigated a single magnetic bogie of the maglev train in this *** magnetic levitation and guidance forces supplied by the suspension modules were deduced by the flux tube *** to the dynamic equilibrium equations of the maglev train on the curved track with cant,several major factors that influence the carrying capacity were analyzed,and the formula of the carrying capacity was *** results provide a theoretical reference for the design of maglev train.

关键词： low-speed maglev train curve flux tube carrying capacity