A fast and effective method to perform paternity testing for Wolong giant pandas

作     者：LI DeSheng CUI HengMin WANG ChengDong LING ShanShan HUANG Zhi ZHANG HeMin 

作者机构：China Research and Conservation Center for the Giant Panda Ya'an 625000 China Medical College of Animal Sichuan Agricultural University Ya'an 625000 China Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and State Conservation Center for Gene Resources of Endangered Wildlife Hangzhou 310058 China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2011年第56卷第24期

页      面：2559-2564页

核心收录：

学科分类：0907[农学-林学] 090705[农学-野生动植物保护与利用] 09[农学] 

基　　金：supported by the National Basic Research Program of China (2007CB411600) a special grant from the State Forestry Administration of China (2130206) the Fundamen-tal Research Funds for the Central Universities of China 

主　　题：圈养大熊猫 亲子鉴定 卧龙 多态性位点 分子生物学技术 多态标记 圈养种群 遗传资源 

摘      要：Captive populations provide a precious genetic resource for endangered animals and a source of individuals for reintroduction to depleted habitats. Therefore, accuracy in determining paternity is of vital importance for managing captive populations and in selecting representative individuals of known genetic characteristics for release. In this study, we established a fast and effective method to conduct paternity testing for captive giant pandas in the Wolong population. This technique uses two highly polymor phic microsatellites initially, subsequent use of five less polymorphic markers and then paternity exclusion testing carried out using the giant panda paternity exclusion program we have developed. Our results revealed that (1) both sets of markers success- fully identified the real fathers in 25 cases of paternity testing and (2) the success rate of paternity exclusion varied with the degree of polymorphism of the markers used. Subsequently, we conducted correlation analysis between the success rates of paterni- ty identification with these markers, parameters of genetic diversity and tests of neutrality. We found that the paternity exclusion power of microsatellites was significantly correlated with the number of alleles (Na), expected heterozygosity (HE) and observed homozygosity statistic (FO) (all P 0.05). From this, we developed a new variable, Na?HE/FO, showing a highly significant positive correlation with the resolution power of microsatellites (P = 0.001). Moreover, the first two highly polymorphic loci gave a 100% success rate of excluding non-paternal males because they yielded higher values of Na?HE/FO than the other five less pol- ymorphic markers. Thus, the Na?HE/FO parameter appears suitable to serve as a criterion for selecting microsatellite markers, which could be used for high-resolution molecular techniques of paternity determination among a range of captive animals besides giant pandas.