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PC-407 inhibited proliferation and induced apoptosis in human colon cancer SW-1116 cells

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Acta Pharmacologica Sinica 2004年第11期 125-130页

作者： li-li HAO, Qi-bing MEI, Bang-le zhang, Min JIA, Xiao-qiang li, feng zhang Department of Pharmacology, The Fourth Military Medical University, Xi-an 710032, China

AIM: To study whether PC-407 [4-[5-naphthyl-3- (trifluoromethyl)-1H-pyrazol-1-yl] benzenesulfonamide] inhibits cell viability and induces apoptosis in human colon cancer SW-1116 cells. METHODS: Inhibition of SW-1116 proliferation was measured by MTT assay. Morphological assessment of apoptosis was performed with fluorescence microscope and electron microscope. DNA fragmentation was visualized by agarose gel electrophoresis. The amount of apoptotic cells was measured by flow cytometry. RESULTS: PC-407 inhibited SW-1116 cell proliferation in a concentration-dependent manner after 3 d of treatment, and the IC50 for PC-407 inhibition of cell number was 16.67±0.17 μmol/L. After incubation of SW-1116 cells with PC-407 20 μmol/L for 24 h, morphological changes of typical apoptosis were observed by AO/EB staining or transmission electron microscopy. Flow cytometry analysis showed that PC-407 induced apoptosis in SW-1116 cells in a time- and concentration-dependant manner. The agarose gel electrophoresis of DN

关键词： prostaglandin-endoperoxide synthase cyclooxygenase inhibitors non-steroidal anti-inflammatory agents apoptosis colonic neoplams

Assessment of deep groundwater over-exploitation in the North China Plain

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Geoscience Frontiers 2011年第4期2卷 593-598页

作者： Jiansheng Shi Zhao Wang Zhaoji zhang Yuhong Fei Yasong li feng＇e zhang Jingsheng Chen Yong Qian Institute of Hydrogeology and Environmental Geology Chinese Academy of Geological Sciences Shigang Street 406 ShijiazhuangHebei 050061 China

A series of environmental—geological problems have been caused by over-exploitation of deep groundwater（i.e.,confined aquifer water） in the North China *** order to better understand the status of deep groundwater over-exploitation and the resultant environmental—geological problems on a regional scale,the over-exploitation of groundwater has been assessed by way of the groundwater exploitation potential coefficient（i.e.,the ratio of exploitable amount of deep groundwater to current exploitation）, cumulative land subsidence,and long-term average lowering rate of the groundwater *** is a good correlation among the results calculated by the different *** a regional scale,deep groundwater has been over-exploited and there is no further exploitation potential under the current *** groundwater exploitation degree index takes the exploitation in 2003 as the reference for the calculations, so the results mainly reflect the degree of current groundwater *** results of over-exploitation of deep groundwater obtained by land subsidence data and long-term average rate of depression of the water table mainly reflect environmental—geological problems caused by exploitation of deep groundwater.

关键词： North China Plain Deep groundwater Assessment of over-exploitation Land subsidence Water table depression

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Mechanical properties and thermal shock resistance of tungsten alloys strengthened by laser fragmentation-processed zirconium carbide nanoparticles

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Tungsten 2020年第4期2卷 381-389页

作者： Ke Jing Chao zhang Rui liu Zhuo‑Ming Xie lin‑Chao zhang li‑feng zhang Jun liu Rui Gao Jun‑feng Yang Xian‑Ping Wang Ting Hao Xue‑Bang Wu Qian‑feng Fang Chang‑Hao liang Guang‑Nan Luo You‑Yun lian Xiang liu Chang‑Song liu Key Laboratory of Materials Physics Institute of Solid State PhysicsHFIPSChinese Academy of SciencesHefei 230031China University of Science and Technology of China Hefei 230026China Institute of Plasma Physics Chinese Academy of SciencesHefei 230031China Southwestern Institute of Physics Chengdu 610041China

Zirconium carbide(ZrC)nanoparticles with an average size of 5.6 nm were synthesized through laser fragmentation(LF)from as-received 20-60 nm ZrC particles,and LF-ZrC nanoparticle dispersion-strengthened tungsten(LF-WZC)samples were fabricated by spark plasma sintering *** average grain size of LF-WZC is 1.91μm and most ZrC particles in LF-WZC are smaller than 10 ***-WZC exhibits finer grain size,higher yield strength and hardness but lower ductility as compared with W-ZrC samples using as-received ZrC(WZC).The results showed that finer ZrC nanoparticles dispersed in tungsten can enhance the strength by hindering the motion of dislocations,but they may also introduce stress concentra-tion and thus reduce the *** thermal shock resistance of the WZC and LF-WZC samples was investigated using an electron beam *** LF-WZC sample also exhibits a higher cracking threshold(0.33-0.44 GW·m^(−2))than WZC(0.22-0.33 GW·m^(−2))at room *** enhanced thermal shock resistance of LF-WZC could be attributed to its high yield strength.

关键词： Tungsten Mechanical properties ZrC Laser fragmentation Thermal shock resistance

Microarray-Based Detection and Differentiation of Virulent and Attenuated Pseudorabies Virus

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Agricultural Sciences in China 2007年第2期6卷 234-243页

作者： liU li-na liU Zheng-fei RUAN li WANG Zhao-xiong li Ren-feng zhang Song-lin CHEN Huan-chun HE Qi-gai Key Laboratory of Preventive Veterinary Medicine of Hubei Province~National Key Laboratory of Agricultural Microbiology~College ofVeterinary Medicine Huazhong Agricultural University Wuhan 430070 P.R.China

DNA microchip used in this study was formed from miniature arrays of pseudorabies virus （PrV） gene-specific probes immobilized on a glass surface. Hybridization using DNA microchip （microarrays） was used for differentiation between virulent and attenuated PrV. The presence of four gene segments （gB, gD, gE~, and gE ） encoding conservative glycoprotein B （gB）, D （gD）, and E （gE） of PrV was monitored using multiplex PCR. The amplicons were labeled with Cy5 or Cy3 dyes followed by hybridization to the gene-specific capture probes on the microchip. The presence of gD and gB, gE~ gene fragments was shown in virulent （gE~ genotype） and attenuated PrV （gE genotype）, whereas gE- gene （deleted domain in gE gene） was demonstrated only in virulent, not in attenuated, virus. No cross-hybridization was observed when fluorescence labeled-PCR products of PrV were hybridized using capture probes of related viruses, such as porcine respiratory and reproductive syndrome virus （PRRSV）, porcine parvovirus （PPV）, Japanese encephalitis virus （JEV）, and porcine circovirus type 2 （PCV-2）. The assay was 10 times sensitive than gD gene-specific PCR. Overall, the results of this study suggested that the microarray might be very useful for detection and differentiation of virulent PrV from attenuated one.

关键词： pseudorabies virus microarray diagnosis differentiation test

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Enhancing wheat regeneration and genetic transformation through overexpression of TaLAX1

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Plant Communications 2024年第5期5卷 9-24页

作者： Yang Yu Haixia Yu Jing Peng Wang Jinsong Yao Yi Peng Wang feng li zhang Shi Rong Wang Yajie Zhao Xiang Yu Zhao Xian Sheng zhang Ying Hua Su State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTai’anShandong 271018China

In the realm of genetically transformed crops,the process of plant regeneration holds utmost ***,the low regeneration efficiency of several wheat varieties currently restricts the use of genetic transformation for gene functional analysis and improved crop *** research explores overex-pression of TaLAX PANICLE1(TaLAX1),which markedly enhances regeneration efficiency,thereby boost-ing genetic transformation and genome editing in *** noteworthy is the substantial increase in regeneration efficiency of common wheat varieties previously regarded as recalcitrant to genetic *** study shows that increased expression of TaGROWTH-REGULATING FACTOR(TaGRF)genes,alongside that of their co-factor,TaGRF-INTERACTING FACTOR 1(TaGIF1),enhances cytokinin accumulation and auxin response,which may play pivotal roles in the improved regeneration and transfor-mation of TaLAX1-overexpressing wheat *** of TaLAX1 homologs also significantly in-creases the regeneration efficiency of maize and soybean,suggesting that both monocot and dicot crops can benefit from this *** shed light on a gene that enhances wheat genetic trans-formation and elucidate molecular mechanisms that potentially underlie wheat regeneration.

关键词： plant regeneration genetic transformation TaLAX1 TaGRF4–TaGIF1 cytokinin auxin wheat

Safety evaluation of donors for living-donor liver transplantation in Chinese mainland:A single-center report

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World Journal of Gastroenterology 2007年第32期13卷 4379-4384页

作者： Guo-Qiang li feng zhang Xiang-Cheng li Bei-Cheng Sun feng Cheng Wen-Gang Ge Xue-Hao Wang liver Transplantation Center First Affiliated Hospital of Nanjing Medical University Nanjing 210029 Jiangsu Province China

AIM： To discuss the safety of donors during living donor liver transplantation （LDLT） and the authors＇ experience with 50 cases. METHODS： Between January 1995 and March 2006, 50 patients with end-stage liver disease received LDLT in our department. Donors （at the age of 27-58 years） were healthy and antibody （ABO）-compatible. The protocol of evaluation and selection of donors, choice of surgical methods and strategy applied in the safety evaluation of donors were analyzed. RESULTS： A total of 115 candidate donors were evaluated for LDLT at our center. Of these, 50 underwent successful hepatectomy for living donation. The elimination rate for donors was 43.5%. Positive hepatitis serology and ABO incompatibility were the main factors for excluding candidates. All donors recovered uneventfully. The follow-up time ranged from 3 to 135 too. The incidence of major and minor medical complications was 12.0% and 28.0%, respectively. CONCLUSION： LDLT provides an excellent approach to the problem of donor shortage in China. With a thorough and complete preoperative workup and meticulous intra-and postoperative management, LDLT can be performed with minimal donor morbidity.

关键词： liver transplantation living donor Safety Evaluation

Neutronics Calculation of ITER HC-SB TBM

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Southwestern Institute of Physics Annual Report

Southwestern Institute of Physics Annual Report 2005年第1期 104-105页

作者： li Zengqiang feng Kaiming zhang Guoshu

ITER＇s test blanket modules （ TBM ） is a test-bed to demonstrate tritium self-sufficiency and extraction of high-grade heat for a future fusion reactor. It is also a test plateform to test electro- magnetic, thermo-hydraulic and tritium breeder for DEMO blanket relevant technologies. A great deal of the largest and the most important nuclear issues are related to neutronics. In consideration of strict requirements of absolute safe operation for ITER and TBM, all of probable or potential problems of TBM must be investigated such as power generation, tritium generation, thermo-hydraulics and energy production and so on.

关键词： Tritium breeding ratio Power density TBM

Advances in the Development of Gradient Scaffolds Made of Nano‑Micromaterials for Musculoskeletal Tissue Regeneration

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Nano-Micro Letters 2025年第3期17卷 455-500页

作者： Lei Fang Xiaoqi lin Ruian Xu Lu liu Yu zhang feng Tian Jiao Jiao li Jiajia Xue Beijing Laboratory of Biomedical Materials Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China State Key Laboratory of Organic‑Inorganic Composites Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China School of Biomedical Engineering Faculty of Engineering and ITUniversity of Technology SydneySydneyNSW 2007Australia

The intricate hierarchical structure of musculoskeletal tissues,including bone and interface tissues,necessitates the use of complex scaffold designs and material structures to serve as tissue-engineered *** has led to growing interest in the development of gradient bone scaffolds with hierarchical structures mimicking the extracellular matrix of native tissues to achieve improved therapeutic *** on the anatomical characteristics of bone and interfacial tissues,this review provides a summary of current strategies used to design and fabricate biomimetic gradient scaffolds for repairing musculoskeletal tissues,specifically focusing on methods used to construct compositional and structural gradients within the *** latest applications of gradient scaffolds for the regeneration of bone,osteochondral,and tendon-to-bone interfaces are ***,the current progress of testing gradient scaffolds in physiologically relevant animal models of skeletal repair is discussed,as well as the challenges and prospects of moving these scaffolds into clinical application for treating musculoskeletal injuries.

关键词： Gradient scaffolds Musculoskeletal tissues Advanced manufacturing Biomaterials Tissue regeneration

A comparison of implantation, miscarriage and pregnancy rates of single and double day 3 embryo transfer between fresh and frozen thawed transfer cycles： a retrospective study

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Chinese Medical Journal 2014年第5期127卷 911-915页

作者： liu liu Tong Xiaomei Jiang lingying Tinchiu li Zhou feng zhang Songying Center of Reproductive Medicine Sir Run Run Shaw HospitalSchool of Medicine Zhejiang University Hangzhou Zhejiang 310006 China Department of Reproductive Medicine Jessop Wing Royal Hallamshire Hospital University of Sheffield UK

Background Reduced endometrial receptivity in hyperstimulated cycles may lead to a lower implantation rate and a lower clinical pregnancy rate,but it is unclear if it is also associated with an increase in pregnancy loss *** aim of this study was to compare the implantation,miscarriage,and pregnancy rates between fresh and frozen thawed transfer of one or two day-3 embryos,with a view to understanding whether or not reduced endometrial receptivity encountered in hyperstimulated cycles is associated with an increase in miscarriage *** This study involved a consecutive series of 1 551 single day-3 embryo transfer cycles and consecutive 5 919 double day-3 embryo transfer cycles in the Assisted Reproductive Unit of the Sir Run Run Shaw Hospital,Hangzhou,China,between January 2010 and December *** The implantation and clinical pregnancy rates （single embryo 30.7％ and double embryos 33.4％ and 51.4％）using fresh cycle were both significantly lower than that of frozen-thawed cycles （single embryo 35.8％ and double embryos 38.1％ and 57.8％）.There was no difference in biochemical loss or clinical miscarriage rates between the two *** Impairment of endometrial receptivity associated with ovarian hyperstimulation leads to implantation failure at a very early stage,resulting in an increased number of *** does not lead to increase in biochemical or clinical *** significantly reduced ongoing pregnancy rates in both fresh single and double embryo transfer are therefore due to failure to achieve a pregnancy,rather than pregnancy loss after conception.

关键词： pregnancy loss miscarriage in vitro fertilization embryo transfer implantation rate

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