Enhancing wheat regeneration and genetic transformation through overexpression of TaLAX1
作者机构：State Key Laboratory of Crop BiologyCollege of Life SciencesShandong Agricultural UniversityTai’anShandong 271018China
出 版 物：《Plant Communications》 (植物通讯（英文）)
年 卷 期：2024年第5卷第5期
页 面：9-24页
核心收录：
基 金：funded by the National Key Research and Development Program of China (2022YFF1002902) the National Natural Science Foundation of China (31730008,32070199) the Natural Science Foundation of Shandong Province (ZR2022JQ12)
主 题：plant regeneration genetic transformation TaLAX1 TaGRF4–TaGIF1 cytokinin auxin wheat
摘 要：In the realm of genetically transformed crops,the process of plant regeneration holds utmost ***,the low regeneration efficiency of several wheat varieties currently restricts the use of genetic transformation for gene functional analysis and improved crop *** research explores overex-pression of TaLAX PANICLE1(TaLAX1),which markedly enhances regeneration efficiency,thereby boost-ing genetic transformation and genome editing in *** noteworthy is the substantial increase in regeneration efficiency of common wheat varieties previously regarded as recalcitrant to genetic *** study shows that increased expression of TaGROWTH-REGULATING FACTOR(TaGRF)genes,alongside that of their co-factor,TaGRF-INTERACTING FACTOR 1(TaGIF1),enhances cytokinin accumulation and auxin response,which may play pivotal roles in the improved regeneration and transfor-mation of TaLAX1-overexpressing wheat *** of TaLAX1 homologs also significantly in-creases the regeneration efficiency of maize and soybean,suggesting that both monocot and dicot crops can benefit from this *** shed light on a gene that enhances wheat genetic trans-formation and elucidate molecular mechanisms that potentially underlie wheat regeneration.
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