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Novel alleles among soybean Bowman-Birk proteinase inhibitor gene families

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science china(Life sciences) 2008年第8期51卷 687-692页

作者： WANG YuePing1,2, CHEN XiongTing2 & qiu lijuan1 1 national key facility for crop gene resources and genetic improvement, key Laboratory of crop Germplasm & Biotechnology(MOA)/institute of crop science, chinese academy of agricultural sciences, beijing 10008, china 2 institute of Tropical Biotechnology, chinese academy of Tropical agricultural sciences, Haikou 571101, china 1. national key facility for crop gene resources and genetic improvement Key Laboratory of Crop Germplasm & Biotechnology (MOA)/Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 10008 China 2. institute of Tropical Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China

Trypsin inhibitors have been found in various animals, plants and *** were two types of trypsin inhibitors in soybean including Bowman-Birk protease inhibitors(BBI) and Kunitz in-hibitors(KTI).The different BBI genes from wild soybean(***) and cultivated soybean(***) formed a multigene *** constructed a cDNA library of cultivar 'SuiNong 14' seed at the R7 growth stage using the SMART *** contigs or singletons were highly homologous to soy-bean protease *** of 5, 35, 8 and 9 were highly homologous to BBI family members BBI-A1, BBI-A2, BBI-C and BBI-D, *** analyses showed there were novel allelic varia-tions among the 4 BBI members in SuiNong *** on the comparison of soybean seed cDNA li-braries from different developmental stages, it was apparent that the expression of trypsin inhibitors increased during seed development in *** analysis of BBI gene sequences among dicotyledonous and monocotyledonous plants demonstrated that these genes shared a common pro-genitor.

关键词： soybean trypsin inhibitor Bowman-Birk proteinase inhibitor Kunitz trypsin inhibitor allelic variation

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Analysis of differential expression proteins of a dwarf soybean mutant by proteomlcs

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Analysis of differential expression proteins of a dwarf soyb...

第十三届全国植物基因组学大会

作者： Wei He,lijuan qiu~* The national key facility for crop gene resources and genetic improvement(NFCRI), institute of crop science,chinese academy of agricultural sciences,100081 beijing,china

Plant height is an important trait for crops and dwarf plant becomes one of important objectives of soybean ***808 is a dwarf mutant derived from Ethylmethylsulfonate(EMS) mutation of soybean cultivar *** this study d...

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MiR1511 co-regulates with miR1511* to cleave the GmRPL4a gene in soybean

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chinese science Bulletin 2012年第28期57卷 3804-3810页

作者： luo zhongqin jin longguo qiu lijuan The national key Faeility for crop gene resources and genetic improvement(NFCRI) Institute of Crop ScienceChinese Academy of Agricultural SeiencesBeijing 100081China

MicroRNA1511(miR1511) is a small RNA with unknown function identified in several plants by deep *** this study,we showed that this small RNA is an authentic miRNA by analyzing the structure of the precursor stem-loop containing the newly identified miR1511 * *** confirmed this result by Northern blotting *** used 5'RACE to identify one of the target genes(GmRPL4a) cleaved by both miR1511 and miR1511 *.The site cleaved by miR1511 * was located in the first exon of GmRPL4a,and the site cleaved by miR1511 was located in the second *** expression level of miR1511/1511 * was higher in leaves than in roots and *** contrast,the lowest level of GmRPL4a expression was in the leaves and the highest in the *** results indicate that an miRNA can co-regulate with an miRNA * to cleave the same target gene in plants,and that the level of GmRPL4a mRNA is regulated by miR1511/1511 *.

关键词：靶基因切割 miRNA Northern 大豆 blot分析小RNA RACE

The optimization of soybean genetic transformation system mediated by Agrobacterium

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The optimization of soybean genetic transformation system me...

第十三届全国植物基因组学大会

作者： Bingfu Guo,lijuan qiu~* The national key facility for crop gene resources and genetic improvement(NFCRI), beijing 100081,china

Soybean[Glycine max(L).Merr]is one of the most important oil crops and the main source of edible vegetable oil and *** has played an important part in soybean breeding and more than 70%of soybean production in the wor...

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SOYBEAN （GLYCINE MAXL.） CORE COLLECTIONS IN china: ITS ESTABLISHMENT AND APPLICATION REVEALED BY BOTH AGRONOMIC CHARACTERS AND SSR MARKERS

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SOYBEAN （GLYCINE MAXL.） CORE COLLECTIONS IN CHINA: ITS EST...

全国作物生物技术与诱变技术学术研讨会

作者： qiu lijuan~(1**), GUAN Rongxia, LIU Zhangxiong, LI Yinghui and CHANG Ruzhen (national key facility of crop gene resources and genetic improvement, institute of crop science, chinese academy of agricultural sciences)

＜正＞ There are over 23 thousands soybean accessions in china, where has the most soybean germplasm in the world. It becomes urgent task to evaluate and utilize the collection efficiently. Based on the passport data and agronomic traits of these germplasm, a total of 20 sampling methods were compared and the best one was subjected to collection samples. The primary core collection was consisted of the samples selecting by the best method and artificially adding few accessions with traits that not included. Then this collection was analyzed by a core set of SSR loci, which identified with 80 autumn soybean accessions and confirmed with 190 random soybean accessions from the whole country. The core collection established with both SSR data

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FLOWING AND MAPPING OF GLYPHOSATE-RESISTANT gene IN SOYBEAN

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FLOWING AND MAPPING OF GLYPHOSATE-RESISTANT GENE IN SOYBEAN

全国作物生物技术与诱变技术学术研讨会

作者： ZHOU Bo, GUAN Rongxia, TAO Bo, CHANG Ruzhen, LIU Zhangxiong, qiu lijuan (national key facility of crop gene resources and genetic improvement, chinese academy of agricultural sciences, 100081, beijing Lab of Weed and Pesticide, Northeast Agriculture University, Haerbin, 150030)

＜正＞ In order to evaluate the glyphosate-resistant gene flowing in different soybean varieties, the glyphosate-resistant soybean（D001） with purple stem and purple flower were planted in the center of field. Around the center in eight directions, three non-glyphosate-resistant soybean with green stem and white flower was planted at the distance 5m, 7m, 10m, 14m, 19m and 25m respectively, including a soybean cultivar zhonghuang15, semi-wild soybean YDD722 and a wild soybeanaccession. The seeds of the three non-transgenic soybean were harvested and then planted for the examination of gene flowing. According to stem color, purple stem seedlings were selected as heterozygote. The wild soybean accession had lowest heterozygote, of which the heterozygous rate is 0 in 64740 individual plants;following with the heterozygous rate of zhonghuang15 foe 1.35% of 40415 ones and YDD722 for 0.05% of 63374 ones. No significant difference of heterozygous rate was observed for the three types of soybeans in different distances or directions by means of variance. And the rate decreased by the increasing of distance, such as 0.58% in 5m and 0.07% in 25m. So the glyphoate-resistant gene of soybean can flow to the non glyphosate-resistant soybean by natural pollination.

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Natural variation of GmFNSII-2 contributes to drought resistance by modulating enzyme activity in soybean

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The crop Journal 2024年第2期12卷 529-539页

作者： Huihui Gao Pengcheng Wei Yongzhe Gu Pengbin Tang Yifan Shen Lei Yang Linxin Dong Haowei Zheng Kuo Shu Mayamiko Masangano Bin Dong Long Miao Jiajia Li lijuan qiu Xiaobo Wang School of Agronomy Anhui Agricultural UniversityHefei 230036AnhuiChina State key Laboratory of crop gene resources and Breeding The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Key Laboratory of Crop Gene Resource and Germplasm Enhancement(MOA)Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

As an essential crop that provides vegetable oil and protein,soybean(Glycine max(L.)Merr.)is widely planted all over the ***,the scarcity of water resources worldwide has seriously impacted on the quality and yield of *** address this,exploring excellent genes for improving drought resistance in soybean is *** this study,we identified natural variations of GmFNSII-2(flavone synthase II)significantly affect the drought resistance of *** sequence analysis of GmFNSII-2 in 632 cultivated and 44 wild soybeans nine haplotypes were *** full-length allele GmFNSII-2^(C),but not the truncated allele GmFNSII-2^(A) possessing a nonsense nucleotide variation,increased enzyme *** research found that GmDREB3,known to increase soybean drought resistance,bound to the promoter region of GmFNSII-2^(C).GmDREB3 positively regulated the expression of GmFNSII-2^(C),increased flavone synthase abundance and improved the drought ***,a singlebase mutation in the GmFNSII-2^(C) promoter generated an additional drought response element(CCCCT),which had stronger interaction strength with GmDREB3 and increased its transcriptional activity under drought *** frequency of drought-resistant soybean varieties with Hap 1(Pro:GmFNSII-2^(C))has increased,suggesting that this haplotype may be selected during soybean *** summary,GmFNSII-2^(C) could be used for molecular breeding of drought-tolerant soybean.

关键词： Drought stress Flavone synthase GmFNSII-2 Natural variation Drought response element

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Pan-genome of annual wild soybean(Glycine soja *** Zucc.)and its comparison with cultivated soybean(***(L.)Merr.)

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Pan-genome of annual wild soybean(Glycine soja *** Zucc.)and...

第十三届全国植物基因组学大会

作者： Yinghui Li~1,Guangyu Zhou~2,Yong Guo~1,Zhouhao Zhang~2,Rongxia Guan~1,Hongmei Zhu~2, Ke jing Wang~1,jinbo Zhang~2,longguo jin~1,Jian Liu~2,Zhangxiong Liu~1,Zhi Jiang~2,Xianghua Li~1,lijuan Zhang~1,Ruiqiang Li~2,lijuan qiu~(1*) 1 The national key facility for crop gene resources and genetic improvement(NFCRI) / institute of crop science,chinese academy of agricultural sciences,100081 beijing,china 2 Novogege Bioinformatics Technology Co.Ltd,beijing,100083 china

As the progenitor of soybean,annual wild soybean(Glycine soja *** Zucc.) is generally considered to be a promising source of novel genes for soybean breeding. By re-sequencing both wild and domesticated soybeans,which taking domesticated Williams 82 genome as a reference(Schmutz et al.,2010,Nature),a wide range of nucleotide and structural variations between wild and domesticated soybean have

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大豆新品种黑农84品质性状的环境稳定性分析

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大豆科学 2024年第1期43卷 1-12页

作者：王婉刘鑫磊孙君明栾晓燕邱丽娟中国农业科学院作物科学研究所/农作物基因资源与遗传改良国家重大科学工程/农业部北京大豆生物学重点实验室北京100081 黑龙江省农业科学院大豆研究所黑龙江哈尔滨150086

为明确黑农84的品质稳定性,为新品种的推广应用提供依据,本研究分析了黑农84不同年份、不同地点种植的品质性状变异。结果显示:黑农84是具备高蛋白、低脂肪、高亚麻酸、高异黄酮、高蔗糖和低水苏糖特性的优质功能型品种。在检测的27个... 详细信息

为明确黑农84的品质稳定性,为新品种的推广应用提供依据,本研究分析了黑农84不同年份、不同地点种植的品质性状变异。结果显示:黑农84是具备高蛋白、低脂肪、高亚麻酸、高异黄酮、高蔗糖和低水苏糖特性的优质功能型品种。在检测的27个品质性状中,16个性状(蛋白质和脂肪含量,蛋脂总量,棕榈酸、硬脂酸、油酸、亚油酸和亚麻酸含量,维生素E含量,水溶性糖,γ-维生素E,δ-维生素E,果糖、葡萄糖、蔗糖和水苏糖含量)的变异系数小于10%,较为稳定。两年的遗传多样性指数变异范围分别为1.08~1.98和1.07~1.73,平均值分别为1.62和1.59,其中α-大豆苷的遗传多样性指数最高(1.98和1.73),棉子糖的遗传多样性指数均最小(1.08和1.07)。黑农84品质性状聚类均可以分为两类,第一类中蛋白质和α-维生素E含量较高,脂肪和异黄酮含量较低;第二类中大豆苷、m-大豆苷、α-大豆苷和m-染料木苷含量较低。黑农84优质性状的环境稳定性为该品种的遗传育种和加工应用等提供了理论依据。

关键词：黑农84 品质性状遗传多样性指数聚类分析环境稳定性

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