MiR1511 co-regulates with miR1511* to cleave the GmRPL4a gene in soybean
MiR1511 co-regulates with miR1511* to cleave the GmRPL4a gene in soybean出 版 物：《Science Bulletin》 (科学通报(英文版))
年 卷 期：2012年第Z2期
页 面：3804-3810页
基 金：supported by the National Natural Science Foundation of China (30871621 and 30490251) the National Major Special Project on New Varieties Cultivation for Transgenic Organisms (2009ZX08009-088B) the National High Technology Research and Development Program of China (2006AA10110)
主 题：soybean miR1511 miR1511 * GmRPL4a
摘 要：MicroRNA1511(miR1511) is a small RNA with unknown function identified in several plants by deep *** this study,we showed that this small RNA is an authentic miRNA by analyzing the structure of the precursor stem-loop containing the newly identified miR1511 * *** confirmed this result by Northern blotting *** used 5’RACE to identify one of the target genes(GmRPL4a) cleaved by both miR1511 and miR1511 *.The site cleaved by miR1511 * was located in the first exon of GmRPL4a,and the site cleaved by miR1511 was located in the second *** expression level of miR1511/1511 * was higher in leaves than in roots and *** contrast,the lowest level of GmRPL4a expression was in the leaves and the highest in the *** results indicate that an miRNA can co-regulate with an miRNA * to cleave the same target gene in plants,and that the level of GmRPL4a mRNA is regulated by miR1511/1511 *.
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