MiR1511 co-regulates with miR1511* to cleave the GmRPL4a gene in soybean

作     者：LUO ZhongQin JIN LongGuo QIU LiJuan 

作者机构：The National Key Faeility for Crop Gene Resources and Genetic Improvement(NFCRI)Institute of Crop ScienceChinese Academy of Agricultural SeiencesBeijing 100081China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2012年第57卷第28期

页      面：3804-3810页

核心收录：

学科分类：0710[理学-生物学] 080503[工学-材料加工工程] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0901[农学-作物学] 0802[工学-机械工程] 0836[工学-生物工程] 090102[农学-作物遗传育种] 080201[工学-机械制造及其自动化] 

基　　金：supported by the National Natural Science Foundation of China (30871621 and 30490251) the National Major Special Project on New Varieties Cultivation for Transgenic Organisms (2009ZX08009-088B) the National High Technology Research and Development Program of China (2006AA10110) 

主　　题：靶基因 切割 miRNA Northern 大豆 blot分析 小RNA RACE 

摘      要：MicroRNA1511(miR1511) is a small RNA with unknown function identified in several plants by deep *** this study,we showed that this small RNA is an authentic miRNA by analyzing the structure of the precursor stem-loop containing the newly identified miR1511 * *** confirmed this result by Northern blotting *** used 5 RACE to identify one of the target genes(GmRPL4a) cleaved by both miR1511 and miR1511 *.The site cleaved by miR1511 * was located in the first exon of GmRPL4a,and the site cleaved by miR1511 was located in the second *** expression level of miR1511/1511 * was higher in leaves than in roots and *** contrast,the lowest level of GmRPL4a expression was in the leaves and the highest in the *** results indicate that an miRNA can co-regulate with an miRNA * to cleave the same target gene in plants,and that the level of GmRPL4a mRNA is regulated by miR1511/1511 *.