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A new Lycopodium alkaloid from Phlegmariurus fargesii

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Chinese Journal of Natural Medicines 2014年第5期12卷 373-376页

作者： PAN Ke luo jian-guang KONG Ling-Yi State Key Laboratory of Natural Medicines Department of Natural Medicinal Chemistry China Pharmaceutical University

AIM: To investigate the chemical constituents from the whole plants of Phlegmariurus fargesii. METHOD: Compounds were isolated by repeated silica gel column chromatography. Their structures were elucidated by spectroscopic methods and chemical correlation. The acetylcholinesterase(AChE) inhibitory activity of the isolated compounds was evaluated. RESULTS: A new Lycopodium alkaloid, lycopodine N-oxide(1), along with lycopodine(2), 8,15-dehydrolycopodine(3), 6α-hydroxylycopodine(4), deacetyllycoclavine(5), N-methylhuperzine B(6), lycodine(7), and phlegmarine(8), was isolated. CONCLUSION: Compound 1 is a new Lycopodium alkaloid, and compound 3 was obtained from nature for the first time. Other alkaloids are isolated from this plant for the first time.

关键词： Phlegmariurusfargesii Lycopodium alkaloids Lycopodine N-oxide

Mutual regulation between microRNA-373 and methyl-CpGbinding domain protein 2 in hilar cholangiocarcinoma

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World Journal of Gastroenterology 2012年第29期18卷 3849-3861页

作者： Yong-Jun Chen jian luo guang-Yao Yang Kang Yang Song-Qi Wen Sheng-Quan Zou Department of Biliary-pancreatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

AIM:To investigate the reciprocal modulation between microRNA(miRNA) and DNA methylation via exploring the correlation between miR-373 and methyl-CpGbinding domain protein(MBD)***:MiR-373 expression was examined using the TaqMan miRNA *** of miR-373 was investigated using methylation-specific polymerase chain reaction,and recruitment of methyl binding proteins was studied using the chromatin immunoprecipitation *** analysis was conducted using the QuikChange Site-Directed Mutagenesis *** activity of miR-373 gene promoter constructs and targeting at MBD2-three prime untranslated region(3'UTR) by miR-373 were evaluated by a dual-luciferase reporter gene ***:In hilar cholangiocarcinoma,miR-373 decreased and was closely associated with poor cell differentiation,advanced clinical stage,and shorter *** promoter-associated CpG island of miR-373 gene was hypermethylated and inhibited expression of ***2 was up-regulated and enriched at the promoter-associated CpG island of ***-mediated suppression of miR-373 required MBD2 enrichment at the promoter-associated CpG island,and miR-373 negatively regulated MBD2 expression through targeting the 3'***:MiR-373 behaves as a direct transcriptional target and negative regulator of MBD2 activity through a feedback loop of CpG island methylation.

关键词： MicroRNA-373 Methyl-CpG binding domain proteins 2 Methylation Hilar cholangiocarcinoma Three prime untranslated region

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巴西甘薯叶黄酮类成分的研究

中国中药杂志 2005年第7期30卷 516-518页

作者：罗建光孔令义中国药科大学江苏南京210038

目的:研究巴西甘薯叶Ipomoeabatatas的黄酮类成分。方法:乙醇提取后分别以氯仿、醋酸乙酯和正丁醇萃取,醋酸乙酯部位用硅胶柱色谱,SephadexLH20凝胶柱色谱等色谱法分离;通过理化性质和波谱方法鉴定结构。结果:分离并鉴定了5个黄酮类化合... 详细信息

目的:研究巴西甘薯叶Ipomoeabatatas的黄酮类成分。方法:乙醇提取后分别以氯仿、醋酸乙酯和正丁醇萃取,醋酸乙酯部位用硅胶柱色谱,SephadexLH20凝胶柱色谱等色谱法分离;通过理化性质和波谱方法鉴定结构。结果:分离并鉴定了5个黄酮类化合物:银椴苷(tiliroside)(Ⅰ),紫云英苷(astragalin)(Ⅱ),鼠李柠檬素(rhamnocitrin)(Ⅲ),鼠李素(rhamnetin)(Ⅳ),山柰酚(Ⅴ)。结论:上述黄酮类成分均首次从该植物中分得。

关键词：黄酮类成分甘薯叶巴西 Sephadex 黄酮类化合物醋酸乙酯正丁醇萃取硅胶柱色谱凝胶柱色谱乙醇提取波谱方法理化性质色谱法紫云英山柰酚分离鉴定

大豆油中黄曲霉毒素B_1快速检测方法的研究

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粮食与油脂 2018年第5期31卷 75-78页

作者：罗建光江苏食品药品职业技术学院食品学院江苏淮安223003

为了探究大豆油中黄曲霉毒素检测的最优方法。通过酶联免疫吸附法和高效液相色谱法相对比,建立了酶联免疫吸附法对大豆油中黄曲霉毒素B1的快速检测方法。试验结果表明:最佳检测条件为振荡器转速200 r/min、振荡时间15 min、显色时间5 mi... 详细信息

为了探究大豆油中黄曲霉毒素检测的最优方法。通过酶联免疫吸附法和高效液相色谱法相对比,建立了酶联免疫吸附法对大豆油中黄曲霉毒素B1的快速检测方法。试验结果表明:最佳检测条件为振荡器转速200 r/min、振荡时间15 min、显色时间5 min、p H 4,在此条件下,检测到大豆油中黄曲霉毒素B_1的含量为2.33μg/kg、平均回收率为99.5%、RSD为0.8%。该方法简便迅速,准确可靠,具有较高的回收率及精密度。

关键词：大豆油黄曲霉毒素高效液相色谱法酶联免疫吸附法

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超临界CO_2提取虾青素的条件优化

粮食与油脂 2018年第12期31卷 54-57页

作者：罗建光江苏食品药品职业技术学院食品学院江苏淮安223003

采用超临界CO_2提取虾青素含量,最佳的提取温度为40℃,最佳的夹带剂为1倍量的95%乙醇,CO_2流量为30L/h,最佳的提取压力为30 MPa,最佳的提取时间为2 h,提取温度为50℃,分离温度为40℃,物料粒径为0.425 mm。线性范围为0～12μg/m L;检出限... 详细信息

采用超临界CO_2提取虾青素含量,最佳的提取温度为40℃,最佳的夹带剂为1倍量的95%乙醇,CO_2流量为30L/h,最佳的提取压力为30 MPa,最佳的提取时间为2 h,提取温度为50℃,分离温度为40℃,物料粒径为0.425 mm。线性范围为0～12μg/m L;检出限为0.01μg/m L。该方法分析灵敏度高、快速准确,精密度和回收率试验结果较好。

关键词：高效液相色谱虾青素萃取

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基于P2P网络的大规模视频直播系统

软件学报 2007年第2期18卷 391-399页

作者：罗建光张萌赵黎杨士强清华大学计算机科学与技术系北京100084

介绍了一种基于P2P(peer-to-peer)网络的大规模视频直播系统Gridmedia.该系统采用Gossip协议构建无结构的应用层覆盖网络,每个节点可以独立地选择自己的伙伴节点.在覆盖网络上,每个节点通过一种推拉结合的流传输策略从邻居节点获取数据.... 详细信息

介绍了一种基于P2P(peer-to-peer)网络的大规模视频直播系统Gridmedia.该系统采用Gossip协议构建无结构的应用层覆盖网络,每个节点可以独立地选择自己的伙伴节点.在覆盖网络上,每个节点通过一种推拉结合的流传输策略从邻居节点获取数据.与DONet中的纯拉策略相比,推拉结合策略大幅度减小了终端用户观看视频的延迟,并有效降低了直播系统的控制开销.PlanetLab上的大量实验充分表明了该策略的有效性.Gridmedia的原型系统通过300Kbps的视频码流对2005年春节联欢晚会进行了全球互联网直播.晚会期间,全球范围内有超过500000人次通过系统观看了直播,最高在线人数达到了15239人,充分验证了系统的性能.

关键词： P2P(peer-to-peer)络视频直播无结构覆盖网络推拉结合策略低延迟

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微波辅助提取黑米中黑色素的研究

粮食与油脂 2018年第11期31卷 73-75页

作者：罗建光鲍会梅江苏食品药品职业技术学院食品学院江苏淮安223003

研究微波辅助提取黑米中黑色素工艺。试验最佳工艺为提取温度70℃、乙醇溶液70%、微波功率320 W、微波辐照时间120s、料液比1∶25(g/m L),微波辅助提取提高了黑米中黑色素的得率且操作时间短、节省试剂用量、污染小、安全。

关键词：高效液相色谱微波黑色素

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超疏水涂膜的研究进展

高分子材料科学与工程 2008年第11期24卷 13-16,20页

作者：黄月文刘伟区罗广建中科院广州化学有限公司广东广州510650

从固体表面的润湿性理论出发介绍了三种不同的接触角模型和理论方程,阐明了实现超疏水性的两个必要条件:低表面能表面和特定的细微粗糙度。同时综述了国内外制备超疏水涂膜时常用的疏水材料和制备粗糙表面的方法、动态疏水性及其应用前景。

关键词：超疏水性润湿接触角粗糙度滑动角

Triterpenoid Saponins from Roots of Gypsophila pacifica

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Chinese Herbal Medicines 2010年第2期2卷 145-147页

作者： NIE Wei,luo jian-guang,KONG Ling-yi Department of Natural Medicinal Chemistry,China Pharmaceutical University,Nanjing 210009,China Department of Natural Medicinal Chemistry China Pharmaceutical UniversityNanjing 210009China

Objective To study the chemical constituents from the roots of Gypsophila *** The chemical constituents were isolated by various column chromatographic methods and their structures were identified by spectral data together with physicochemical *** Five compounds were isolated and identified as 3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl gypsogenin 28-O-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside(1),3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl quillaic acid 28-O-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside(2),3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl gypsogenin 28-O-β-D-glucopyranosyl-(1→3)-[β-D-xylopyranosyl-(1→4)]-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside(3),3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl quillaic acid 28-O-β-D-glucopyranosyl-(1→3)-[β-D-xylopyranosyl-(1→4)]-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside(4),3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl quillaic acid 28-O-α-L-arabino-pyranosyl-(1→4)-α-L-arabinopyranosyl-(1→3)-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside(5).Conclusion The five compounds are isolated from this plant for the first time.

关键词： Caryophyllaceae Gypsophila pacifica triterpenoid saponins