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Development of a recombinant pB602L-based indirect ELISA assay for detecting antibodies against African swine fever virus in pigs

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Journal of Integrative Agriculture 2022年第3期21卷 819-825页

作者： WANG Peng-fei WANG Ming SHI Zhi-bin SUN Zhen-zhao WEI Li-li liu zai-si WANG Shi-da HE Xi-jun WANG Jing-fei State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China

African swine fever(ASF),caused by the African swine fever virus(ASFV),is a devastating disease of domestic and wild *** is no effective vaccine,and the control of the disease relies mainly on surveillance and early detection of infected ***,serological assays,such as ELISA,have been developed mainly based on recombinant structural viral proteins of ASFV,including p72,p54,and ***,the antibodies against these proteins do not provide efficient protection against ASFV infection in ***,new serological assays that can be applied for clinical diagnosis and evaluating serological immune response in vaccinated pigs are still *** this study,we expressed and purified a recombinant p B602 L *** purified p B602 L protein was then used as an antigen to develop an indirect ELISA *** assay has no cross-reaction with the anti-sera against the 15 most common pig pathogens in China,such as classical swine fever virus,pseudorabies virus,and porcine *** assay and a commercial ELISA kit were then used to detect 60 field pig serum samples,including an unknown number of antiASFV *** coincidence of the two assays was 95%.Furthermore,the p B602 L-based ELISA was employed to test the antibody responses to the seven-gene-deleted ASFV strain HLJ/18-7 GD in *** results showed that the antibody levels in all vaccinated pigs,starting from the 10 th day post-inoculation,have increased continuously during the observation period of 45 *** results indicate that this p B602 L-based indirect ELISA assay can be employed potentially in the field of ASFV diagnosis.

关键词： African swine fever virus pB602L recombinant protein indirect ELISA assay pig

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Unraveling brain aging through the lens of oral microbiota

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Neural Regeneration Research 2025年第7期20卷 1930-1943页

作者： Qinchao Hu si Wang Weiqi Zhang Jing Qu Guang-Hui liu State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Hospital of Stomatology Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijingChina CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijingChina University of Chinese Academy of Sciences BeijingChina Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina Aging Biomarker Consortium BeijingChina State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Beijing Institute for Stem Cell and Regenerative Medicine BeijingChina

The oral cavity is a complex physiological community encompassing a wide range of *** of oral microbiota can lead to various oral infectious diseases,such as periodontitis and tooth decay,and even affect systemic health,including brain aging and neurodegenerative *** studies have highlighted how oral microbes might be involved in brain aging and neurodegeneration,indicating potential avenues for intervention *** this review,we summarize clinical evidence demonstrating a link between oral microbes/oral infectious diseases and brain aging/neurodegenerative diseases,and dissect potential mechanisms by which oral microbes contribute to brain aging and *** also highlight advances in therapeutic development grounded in the realm of oral microbes,with the goal of advancing brain health and promoting healthy aging.

关键词： Alzheimer's disease brain aging multiple sclerosis neurodegeneration neurodegenerative diseases oral microbiota Parkinson's disease periodontitis bacteria Porphyromonas gingivalis

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NOX4 exacerbates Parkinson's disease pathology by promoting neuronal ferroptosis and neuroinflammation

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Neural Regeneration Research 2025年第7期20卷 2038-2052页

作者： Zhihao Lin Changzhou Ying Xiaoli si Naijia Xue Yi liu Ran Zheng Ying Chen Jiali Pu Baorong Zhang Department of Neurology The Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang ProvinceChina

Parkinson's disease is primarily caused by the loss of dopaminergic neurons in the substantia nigra ***,a novel form of regulated cell death characterized by iron accumulation and lipid peroxidation,plays a vital role in the death of dopaminergic ***,the molecular mechanisms underlying ferroptosis in dopaminergic neurons have not yet been completely *** oxidase 4 is related to oxidative stress,however,whether it regulates dopaminergic neuronal ferroptosis remains *** aim of this study was to determine whether NADPH oxidase 4 is involved in dopaminergic neuronal ferroptosis,and if so,by what *** found that the transcriptional regulator activating transcription factor 3 increased NADPH oxidase 4 expression in dopaminergic neurons and astrocytes in an 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine-induced Parkinson's disease *** oxidase 4 inhibition improved the behavioral impairments observed in the Parkinson's disease model animals and reduced the death of dopaminergic ***,NADPH oxidase 4 inhibition reduced lipid peroxidation and iron accumulation in the substantia nigra of the Parkinson's disease model ***,we found that NADPH oxidase 4 interacted with activated protein kinase Cαto prevent ferroptosis of dopaminergic ***,by lowering the astrocytic lipocalin-2 expression,NADPH oxidase 4 inhibition reduced 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine-induced *** findings demonstrate that NADPH oxidase 4 promotes ferroptosis of dopaminergic neurons and neuroinflammation,which contribute to dopaminergic neuron death,suggesting that NADPH oxidase 4 is a possible therapeutic target for Parkinson's disease.

关键词： dopaminergic neuron ferroptosis NADPH oxidase 4(NOX4) neuroinflammation Parkinson's disease

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科学数据伦理治理:政策框架与路径——以英国为例

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现代情报 2025年第1期45卷 112-123,134页

作者：刘先瑞司莉武汉大学信息管理学院湖北武汉430072

[目的/意义]完善科学数据伦理治理有助于规范科学数据收集、共享和使用,发挥数据作为科研的关键资源与要素的功能,优化科学数据管理服务。[方法/过程]本研究基于扎根理论分析英国科学数据伦理政策,总结其政策框架与内容,并通过矩阵编码... 详细信息

[目的/意义]完善科学数据伦理治理有助于规范科学数据收集、共享和使用,发挥数据作为科研的关键资源与要素的功能,优化科学数据管理服务。[方法/过程]本研究基于扎根理论分析英国科学数据伦理政策,总结其政策框架与内容,并通过矩阵编码与社会网络分析方法揭示治理特征,提出我国优化科学数据伦理治理的建议。[结果/结论]研究发现,伦理政策框架包括治理环境、主体与策略3层面。伦理治理环境内容分为适用领域、适用主体、概念定义、问题分析与现有政策;伦理治理主体内容涉及治理主体界定与促进主体协作;伦理治理策略内容涵盖治理指导与各阶段治理措施。其治理特征有3维度:注重定义概念,明确分配主体权责与促进协作,全面厘定治理策略与加强伦理风险防控。据此提出4条治理路径:形成治理体系,规范治理理论,明确各方权责与协作方式,细化各阶段治理措施。

关键词：科学数据数据伦理数据治理政策分析英国

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Distinct MLT asymmetry of auroral kilometric radiation observed by the FAST satellite

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Earth and Planetary Physics 2025年第1期9卷 188-193页

作者： JiaWen Tang Sai Zhang FuLiang Xiao HongMing Yang si liu YiHua He Chang Yang YuYue Jin ZhouKun Deng Ping Li Archie James Johnston School of Physics and Electronic Sciences Changsha University of Science and TechnologyChangsha 410114China Hunan Province Higher Education Key Laboratory of Modeling and Monitoring on the Near-Earth Electromagnetic Environments Changsha University of Science and TechnologyChangsha 410114China School of Electrical and Information Engineering Changsha University of Science and TechnologyChangsha 410114China

Auroral Kilometric Radiation(AKR)is a common radio emission,which can contribute to the magnetosphere−ionosphere−atmosphere *** emissions have been observed in all magnetic planet magnetospheres of the solar *** this study,using observations from the FAST satellite from 30 August 1996 to 9 September 2001,the distribution of AKR in altitude=500−4500 km and invariant latitude(|ILAT|)=60°−80°has been analyzed.63045 AKR samples have been identified with~48%(52%)samples on the dayside(nightside).Of considerable interest,there is a distinct MLT asymmetry with the high occurrence rate in MLT=05−08 and 18−22(02−05 and 12−17)in the northern(southern)*** distinct MLT asymmetry is associated with the direction of Bxof the interplanetary magnetic *** addition,the occurrence rate on the nightside clearly increases as the index *** study further enriches *** and understanding of AKR in the magnetosphere as well as other similar radio emissions.

关键词： asymmetry auroral kilometric radiation interplanetary magnetic field

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Momentum Effect Differs Across Stock Performances:Chinese Evidence

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Acta Mathematicae Applicatae sinica 2014年第2期30卷 279-288页

作者： Zhao-yuan LI si-bo liu Mao-zai TIAN Department of Statistics and Actuarial Science The University of Hong Kong Department of Finance and Insurance Lingnan University Center for Applied Statistics School of StatisticsRenmin University of China School of Statistics Lanzhou University of Finance and Economics

Prior empirical studies find positive and negative momentum effect across the global nations, but few focus on explaining the mixed results. In order to address this issue, we apply the quantile regression approach to analyze the momentum effect in the context of Chinese stock market in this paper. The evidence suggests that the momentum effect in Chinese stock is not stable across firms with different levels of performance. We find that negative momentum effect in the short and medium horizon （3 months and 9 months） increases with the quantile of stock returns. And the positive momentum effect is observed in the long horizon （12 months）, which also intensifies for the high performing stocks. According to our study, momentum effect needs to be examined on the basis of stock returns. OLS estimation, which gives an exclusive and biased result, provides misguiding intuitions for momentum effect across the global nations. Based on the empirical results of quantile regression, effective risk control strategies can also be inspired by adjusting the proportion of assets with past performances.

关键词： chinese stock market investment strategy momentum effect quantile regression

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禽流感病毒NS1蛋白在杆状病毒表达系统中的表达

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动物医学进展 2012年第6期33卷 27-31页

作者：李东卫郭莹莹刘在斯王世达沈楠文辉强焦同路王靖飞中国农业科学院哈尔滨兽医研究所动物疫病诊断中心农业部兽用药物与兽医生物技术重点实验室黑龙江哈尔滨150001 东北农业大学动物医学学院黑龙江哈尔滨150030

为获得纯化的禽流感病毒(AIV)的NS1蛋白,将A/chicken/Guangxi/10/99(H9N2)(CK/GX/10/99)NS1全长核苷酸克隆至杆状病毒转移载体pFastBacHTA中,构建了重组转移载体pFastBacHTA-NS1,然后转化入DH10Bac感受态细胞中制备重组杆粒,在脂质体介导下转染sf9昆虫细胞,获得表达NS1基因的重组杆状病毒。用重组杆状病毒转染昆虫细胞sf9进行NS1蛋白的表达,通过SDS-PAGE电泳、Western blot、间接免疫荧光(IFA)对表达的目标蛋白进行分析。结果表明,构建了含NS1基因的重组杆状病毒,分子质量约30ku的重组蛋白得到了表达,为NS1的相关功能研究奠定了基础。

关键词：禽流感病毒,NS1蛋白杆状病毒表达载体系统蛋白纯化

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Low-cost and fluoride-free synthesis of MFI zeolite nanosheets with enhanced stability for benzene alkylation with ethanol

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Journal of Energy Chemistry 2025年第1期100卷 458-468页

作者： Peng Zhu Cun liu Yiren Yu Guoshu Gao Yumeng Zhao Xiongfu Zhang Guodong liu Guohui Yang State Key Laboratory of Fine Chemicals School of Chemical Engineering Dalian University of Technology State Key Laboratory of Catalysis Dalian Institute of Chemical Physics Chinese Academy of Sciences

Zeolite nanosheets with a short b-axis thickness are highly desirable in lots of catalytic reactions due to their reduced diffusion resistance. Nevertheless, conventional synthesis methods usually require expensive structure-directing agents(SDAs), pricey raw materials, and eco-unfriendly fluorine-containing additives. Here, we contributed a cost-effective and fluoride-free synthesis method for synthesizing high-quality MFI zeolite nanosheets through a silicalite-1(sil-1) seed suspension and urea cooperative strategy, only with inexpensive colloidal silica as the si source. Our approach was effective for synthesizing both sil-1 and aluminum-containing ZSM-5 nanosheets. By optimizing key synthesis parameters,including seed aging time, seed quantity, and urea concentration, we achieved precise control over the crystal face aspect ratio and b-axis thickness. We also revealed a non-classical oriented nanosheet growth mechanism, where sil-1 seeds induced the formation of quasi-ordered precursor particles, and the(010)crystal planes of these particles facilitated urea adsorption, thereby promoting c-axis-oriented *** obtained ZSM-5 nanosheets exhibited exceptional catalytic performance in the benzene alkylation with ethanol, maintaining stability for over 500 h, which is 5 times longer than traditional ZSM-5 catalysts. Furthermore, large-scale production of ZSM-5 nanosheets was successfully carried out in a 3 L highpressure autoclave, yielding samples consistent with those from laboratory-scale synthesis. This work marks a significant step forward in the sustainable and efficient production of MFI nanosheets using inexpensive and environmentally friendly raw materials, offering the broad applicability in catalysis.

关键词： MFI zeolite ZSM-5 Nanosheets Scale-up synthesis Aromatic alkylation

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A review on Ti_(3)C_(2)T_(x)-based nanomaterials:synthesis and applications in gas and humidity sensors

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Rare Metals 2021年第6期40卷 1459-1476页

作者： Qiu-Ni Zhao Ya-Jie Zhang zai-Hua Duan si Wang Can liu Ya-Dong Jiang Hui-Ling Tai State Key Laboratory of Electronic Thin Films and Integrated Devices School of Optoelectronic Science and EngineeringUniversity of Electronic Science and Technology of ChinaChengdu 610054China

Ti_(3)C_(2)T_(x),which is a novel two-dimensional(2 D)material,has received enormous interest in the field of sensor technology due to its large surface area,excellent electrical conductivity,and abundant active surface *** recent years,several Ti_(3)C_(2)T_(x)-based gases and humidity sensors have been developed and *** this review,we focus on the latest applications of Ti_(3)C_(2)T_(x)-based nanomaterials in gas and humidity ***,the synthesis of Ti_(3)C_(2)T_(x) from the dangerous fluorine-containing etching process to the safe fluorine-free preparation method was discussed,and the structures of the Ti_(3)C_(2)T_(x) controlled by different delamination methods were also ***,the functionalization of pristine Ti_(3)C_(2)T_(x) and composite strategies for enhancing its gas and humidity sensing performance were *** addition,the gas and humidity sensing mechanisms of sensors based on Ti_(3)C_(2)T_(x) were also ***,the challenges and opportunities for the use of Ti_(3)C_(2)T_(x) gas and humidity sensors were discussed to provide guidance on the promising potential of Ti_(3)C_(2)T_(x) in this field.

关键词： Ti_(3)C_(2)T_(x) Two-dimensional material Synthesis Gas sensor Humidity sensor

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On a risk model with Markovian arrivals and tax

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Applied Mathematics(A Journal of Chinese Universities) 2012年第2期27卷 150-158页

作者： DONG Hua liu zai-ming School of Mathematics Qufu Normal UniversityQufu 273165China School of Mathematics Central South UniversityChangsha 410075China

A risk model with Markovian arrivals and tax payments is *** the insurer is in a profitable situation,the insurer may pay a certain proportion of the premium income as tax ***,the Laplace transform of the time to cross a certain level before ruin is ***,explicit formulas for a generalized Gerber-Shiu function are established in terms of the＇original＇Gerber-Shiu function without tax and the Laplace transform of the first passage time before ***,the differential equations satisfied by the expected accumulated discounted tax payments until ruin are *** explicit expression for the discounted tax payments is also given.

关键词： Markovian arrival process Gerber-Shiu function tax payment.

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