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Identification of leaf rust resistance genes in common wheat varieties from China and foreign countries

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Journal of Integrative Agriculture 2021年第5期20卷 1302-1313页

作者： liU Yuan Takele Weldu GEBREWAHID ZHANG Pei-pei li zai-feng liU Da-qun College of Plant Protection Hebei Agricultural UniversityBaoding 071001P.R.China College of Agriculture Aksum UniversityShire-Indaslassie 314Ethiopia

Wheat leaf rust,triggered by Puccinia triticina Eriks(Pt),is among the most important diseases of wheat *** resistant varieties against leaf rust is the most effective,environmentally-friendly and economic way to control the *** the present study,66 wheat varieties form China and foreign countries were tested with 17 Pt races for gene postulation during the seedling stage in the *** the varieties were also planted to identify slow rusting responses to leaf rust at the adult plant stage in Baoding and Zhoukou field trials during the 2016/2017 to 2017/2018 cropping ***,12 closely linked molecular markers to known leaf rust resistance(Lr)genes were used for assessing all the *** results of both gene postulation and molecular marker identification showed that a total of eight Lr genes,Lr1,Lr10,Lr17,Lr20,Lr26,Lr34,Lr37 and Lr46,either singly or in combination were detected in 32 *** Lr genes were not identified in the remaining 34 *** varieties were found to have slow rusting *** resistance sources identified in this study can be used as resources for resistance against leaf rust in wheat breeding programs in China and the respective foreign countries.

关键词： wheat gene postulation molecular marker Lr gene

Seedling and adult plant resistance to leaf rust in 46 Chinese bread wheat landraces and 39 wheat lines with known Lr genes

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Journal of Integrative Agriculture 2019年第5期18卷 1014-1023页

作者： ZHANG Pei-pei Takele Weldu Gebrewahid ZHOU Yue li Qing-luo li zai-feng liU Da-qun Department of Plant Pathology College of Plant Protection Hebei Agricultural University College of Agriculture Aksum University-Shire Campus College of Biochemistry and Environmental Engineering Baoding University

Wheat leaf rust,caused by Puccinia triticina(Pt),is an important foliar disease that has an important influence on wheat *** most economic,safe and effective way to control the disease is growing resistant *** the present study,a total of 46 wheat landraces and 34 wheat lines with known Lr(leaf rust resistance)genes were inoculated with 16Pt pathotypes for postulating seedling resistance gene(s)in the *** cultivars and five wheat differential lines with adult plant resistance(APR)genes(Lr12,Lr22b,Lr34,Lr35 and Lr37)were also evaluated for identification of slow rusting resistance in the field trials in Baoding,Hebei Province of China in the 2014–2015 and 2015–2016 cropping ***,10 functional molecular markers closely linked to 10 known Lr genes were used to detect all the wheat *** showed that most of the landraces were susceptible to most of the Pt pathotypes at seedling ***,Lr1 was detected only in *** field experimental test of the two environments showed that 38 landraces showed slow rusting *** cultivars possessed Lr34 but none of the landraces contained Lr37 and *** genes namely,Lr9,Lr19,Lr24,Lr28,Lr29,Lr47,Lr51 and Lr53 were effective at the whole plant ***18,Lr36 and Lr45 had lost resistance to part of pathotypes at the seedling stage but showed high resistance at the adult plant ***34 as a slowing rusting gene showed good resistance in the *** race-specific APR genes Lr12,Lr13,Lr35 and Lr37 conferred good resistance in the field *** race-specific genes,Lr2b,Lr2c,Lr11,Lr16,Lr26,Lr33 and LrB had lost *** 38 landraces showed slow rusting resistance to wheat leaf rust can be used as resistance resources for wheat resistance breeding in China.

关键词： Triticum aestivum L. wheat leaf rust gene postulation adult plant resistance

SYNTHESIS OF POLYURETHANE MODIFIED BISMALEIMIDE (UBMI) AND POLYURETHANE-IMIDE ELASTOMER

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Chinese Journal of Polymer Science 2008年第1期26卷 117-119页

作者： Xu, Tao Yuan, Wei Wang, Shen-jun li, zai-feng Sun, Bao-quan Wang, Min-xuan Qingdao Univ Sci & Technol Coll Chem & Mol Engn Qingdao 266042 Peoples R China SINOPEC Shengli Petr Adm Inst Oil Proc Shengli Oilfield Dongying 257001 Peoples R China

Urethane modified bismaleimide(UBMI)was synthesized by the reaction of maleic anhydride(MA)with NCO group terminated polyurethane prepolymer(PUP)in presence of *** product was determined by infrared analysis. Then ultrasonic assistant process was introduced into the solvent removal of the prepolymer mixture of UBMI and PUP. Polyurethane-imide(PUI)elastomer was synthesized from the above PUP-UBMI prepolymer mixture by the infusion technology with 2,5-dimethyl-2,5-bis(tert-butylperoxy)-hexane(B25)as l...

关键词： UBMI Polyurethane-imide Preparation

Mapping of QTL conferring leaf rust resistance in Chinese wheat lines W014204 and Fuyu 3 at adult plant stage

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Journal of Integrative Agriculture 2016年第1期15卷 18-28页

作者： QI Ai-yong ZHANG Pei-pei ZHOU Yue YAO Zhan-jun li zai-feng liU Da-qun Department of Plant Pathology College of Plant Protection Agricultural University of Hebei Department of Biochemistry Baoding University College of Agronomy Agricultural University of Hebei

Wheat leaf rust is a destructive foliar disease of common wheat （Triticum aestivum L.） worldwide. The most effective, economical s to control the disease is growing resistant cultivars with adult plant resistance （APR）. The Chinese wheat tines W014204 and Fuyu 3 showed high leaf rust resistance in the field. To identify leaf rust APR genes in the two lines, two mapping populations with 215 and 163 F2：3 lines from the crosses W014204/Zhengzhou 5389 and Fuyu 3/Zhengzhou 5389, respectively, were phenotyped for leaf rust severities during the 2010-2011, 2011-2012 and 2012-2013 cropping seasons in the field at Baoding, Hebei Province, China. A total of 1 215 SSR markers were used to identify the quantitative trait loci （QTLs） for leaf rust APR in the two populations. In the W014204/Zhengzhou 5389 population, three QTLs were detected and designated as ***-lBL.1, ***-2BS.1 and ***-7DS, and explained 2.9-8.4, 11.5-38.3 and 8.5-44.5% of the phenotypic variance, respectively; all the resistance alleles at these loci were derived from W014204. In the Fuyu 3/Zhengzhou 5389 population, three QTLs, ***-lBL.2, ***-2BS.2 and ***-7BL, explained 12.0-19.2, 22.3-38.9 and 4.1-4.3% of the phenotypic variance, respectively, and all resistance alleles were contributed by Fuyu 3. Based on chromosome positions of closely linked markers, both ***-lBL. 1 and ***-lBL.2 are Lr46, and ***-7DS is Lr34. ***-7BL was mapped on chromosome 7BL near to Lr68 and they are likely the same gene. Based on chromosome positions, pedigree and field reactions, the two 2BS QTLs are different from all the known APR genes and are likely to be newAPR QTL for leaf rust. These QTLs and their closely linked markers are potentially useful for improving leaf rust resistance in wheat breeding.

关键词： Triticum aestivum Puccinia triticina APR SSR marker

Molecular mapping of leaf rust resistance genes in the wheat line Yu 356-9

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Journal of Integrative Agriculture 2015年第7期14卷 1223-1228页

作者： HAN liu-sha li zai-feng WANG Jia-zhen SHI ling-zhi ZHU lin li Xing liU Da-qun Syed J A Shah College of Plant Protection Agricultural University of Hebei Nuclear Institute for Food and Agricuture

The Chinese wheat line Yu 356-9 exhibits a high level of resistance to leaf rust. In order to decipher the genetic base of resistance in Yu 356-9, gene postulation, inheritance analyses, and chromosome linkage mapping were carried out. Gene postulation completed using 15 leaf rust pathotypes and 36 isogenic lines indicated that Yu 356-9 was resistant to all pathotypes tested. F1 and F2 plants from the cross Yu 356-9 （resistant）/Zhengzhou 5389 （susceptible） were tested with leaf rust pathotype ＂FHNQ＂ in the greenhouse. Results indicated a 3：1 segregation ratio, indicative of the presence of a single dominant leaf rust resistance gene in Yu 356-9 which was temporarily designated as LrYu. Bulk segregant analysis and molecular marker assays were used to map LrYu. Five simple sequence repeat （SSR） markers on chromosome 2BS were found closely linked to LrYu. Among these markers, Xwmc770 is the most closely linked, with a genetic distance of 5.7 cM.

关键词： wheat leaf rust resistance gene inheritance analyses molecular mapping

Robust chemo-mechanical stability of additives-free SiO_(2)anode realized by honeycomb nanolattice for high performance li-ion batteries

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Chinese Chemical Letters 2024年第10期35卷 442-446页

作者： Xinpin Pan Yongjian Cui Zhe Wang Bowen li Hailong Wang Jian Hao feng li Jing li Advanced Energy Storage Materials Lab School of Materials and New EnergyNingxia UniversityYinchuan 750021China School of Chemistry and Chemical Engineering Ningxia UniversityYinchuan 750021China

Silicon-based anodes including Si,Si Oxand SiO_(2)could deliver ultra-large capacities,but degrade fast owing to huge volume change and low ***,large amounts of elastic binder and conductive additives were composited with nanosized silicon-based materials to yield reasonable cycling stability,which nevertheless not only decrease specific capacity but also induce inhomogeneous lithiation/delithiation as well as uneven stress *** nanolattice has exhibited superior mechanical properties which could be ideal structure for silicon-based anodes,but yet faces challenges in integration of chemical reactivity,conductivity and mechanical ***,we fabricate artificial SiO_(2)honeycomb nanolattice consisting of numerous nanoscale SiO_(2)cells interconnected by through-holes,and conformal coating of highly graphitic carbon on the nanolattice is achieved through in situ catalytic ***,the nanolattice is firmly bonded on Cu substrate through atomic interdiffusion irrespective of surface *** unique structure allows fast charge transportation and homogeneous lithiation/delithiation throughout the micron-meter nanolattice,which results in excellent stability and large reversible capacity over 500 cycles at 1 A/*** results highlight design and constructing artificial nanolattice can be an effective way to prevent chemo-mechanical degradation of silicon-based anode materials.

关键词： li-ion batteries Nanolattice SiO_(2)anode Stability Conductivity

Discovery and Asymmetric Total Synthesis of Phlegmine A as a Selective Inhibitor of ASIC1a

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CCS Chemistry 2024年第10期6卷 2529-2536页

作者： zai-feng Yuan Fei liu Yu-Fei Ou Wen-Yan li li-Yan Peng Xiao-Nian li Qin-Shi Zhao State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201 School of Pharmacy Chengdu University of Traditional Chinese MedicineChengdu 611137 University of Chinese Academy of Sciences Beijing 10049

Phlegmine A(1),a novel type of Lycopodium alkaloid with a unique skeleton,was isolated from the rare and endangered herbal medicinal plant,Phlegmariurus *** methods and X-ray diffraction analysis were employed to unambiguously elucidate the structure of phlegmine A(1).Furthermore,we realized the asymmetric total synthesis of the natural product phlegmine A(1)in 18 linear steps from commercial ingredients,achieving a 1.3%overall *** intramolecular carbene cyclization,dimethyldioxirane(DMDO)enamine oxidation,and Stevens rearrangement were exploited to establish the sterically congested vicinal quaternary centers,and an epimerization/aldol condensation/deacetylation reaction was utilized for the rapid assembly of enone at the final *** our semisynthesis attempts,a novel transformation was developed to constructα-aminoketone from enamine ***,the synthetic sample obtained from this work enabled the successful verification of phlegmine A(1)as a new type of highly selective acidsensing ion channel 1a(ASIC1a)inhibitor,which also exerted an in vivo analgesic effect,rendering it a promising lead compound.

关键词： Lycopodium alkaloids phlegmine A total synthesis ASIC1a inhibitor analgesic

Pd cluster decorated free standing flexible cathode for high performance li-oxygen batteries

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Nano Research 2024年第4期17卷 2678-2686页

作者： liang Guo Guoliang Zhang Ruonan Yang Dongmei Zhang Xiuqi Zhang Han Yu Xia li feng Dang Key Laboratory for liquid-Solid Structure Evolution and Processing of Materials Ministry of EducationShandong UniversityJinan 250061China

As a promising candidate for the next generation energy storage system,rechargeable lithium-oxygen batteries(LOBs)still face substantial challenges caused by insulating discharge products that preclude their practical *** highly efficient cathode catalysts capable of facilitating formation/decomposition of discharge products is considered as an essential approach towards high performance ***,Pd decorated Te nanowires(Pd@Te NWs)were synthesized as advanced catalyst in LOBs to maximize Pd utilization and achieve synergistic effect,in which Pd clusters were uniformly grown on Te substrate though regulating the Pd:Te ***,Pd@Te nanowires assembled into an interpenetrating network-like structure by vacuum filtration and employed as flexible cathode,enabling LOBs achieved an ultralong 190 cycles stability and a superior specific capacity of 3.35 mAh·cm^(-2).Experimental studies and density functional theory(DFT)calculations reveal the excellent catalytic ability of Pd@Te and synergistic catalytic mechanism of Pd and Te,in which uniform electron distribution,extensive electron exchange,and large adsorption distance between Pd cluster and discharge products promote homogeneous adsorption/desorption of discharge products,while the high adsorption energy of Te substrate for li species reduces the initial dynamical energy barrier during discharging *** current work provides viable strategy to design composite catalysts for flexible cathode of LOBs with synergistic catalytic effects.

关键词： flexible lithium-oxygen batteries Pd@Te synergistic catalysis cathode catalysts density functional theory(DFT)calculation

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咪唑和三唑醚类化合物的合成及生物活性

精细化工 2005年第8期22卷 619-624页

作者：李再峰罗富英湛江师范学院自然科学与技术研究中心广东湛江524048

以2,4-二氯-α-氯代苯乙酮、取代甲苯、咪唑、三唑为起始原料,合成了9个咪唑醚类和16个三唑醚类化合物,所有化合物结构经1HNMR、IR和元素分析进行了确证,对化合物对马铃薯晚疫病菌(Phytophthoro infestans)和秧苗枯萎病菌(Phizoctonia)... 详细信息

以2,4-二氯-α-氯代苯乙酮、取代甲苯、咪唑、三唑为起始原料,合成了9个咪唑醚类和16个三唑醚类化合物,所有化合物结构经1HNMR、IR和元素分析进行了确证,对化合物对马铃薯晚疫病菌(Phytophthoro infestans)和秧苗枯萎病菌(Phizoctonia)的抑菌活性进行了测定,测定表明,25个化合物都有不同程度的抑菌活性,其中Ⅲa、Ⅲb、Ⅲd、Ⅳg、Ⅴb、Ⅴc等6个化合物质量浓度为50 mg/L和10 mg/L时,对两种病菌的抑菌率达80%和50%以上,初步分析了化合物抑菌活性与结构的关系。

关键词：咪唑醚类化合物三唑醚类化合物抑菌活性构效关系