Molecular mapping of leaf rust resistance genes in the wheat line Yu 356-9

作     者：HAN Liu-sha LI Zai-feng WANG Jia-zhen SHI Ling-zhi ZHU Lin LI Xing LIU Da-qun Syed J A Shah 

作者机构：College of Plant Protection Agricultural University of Hebei Nuclear Institute for Food and Agricuture 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2015年第14卷第7期

页      面：1223-1228页

核心收录：

学科分类：09[农学] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 0904[农学-植物保护] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China (International/Regional Cooperation and Exchange Program) (31361140367) the Hebei Provincial Outstanding Youth Project, China (YQ2013024) 

主　　题：wheat leaf rust resistance gene inheritance analyses molecular mapping 

摘      要：The Chinese wheat line Yu 356-9 exhibits a high level of resistance to leaf rust. In order to decipher the genetic base of resistance in Yu 356-9, gene postulation, inheritance analyses, and chromosome linkage mapping were carried out. Gene postulation completed using 15 leaf rust pathotypes and 36 isogenic lines indicated that Yu 356-9 was resistant to all pathotypes tested. F1 and F2 plants from the cross Yu 356-9 （resistant）/Zhengzhou 5389 （susceptible） were tested with leaf rust pathotype ＂FHNQ＂ in the greenhouse. Results indicated a 3：1 segregation ratio, indicative of the presence of a single dominant leaf rust resistance gene in Yu 356-9 which was temporarily designated as LrYu. Bulk segregant analysis and molecular marker assays were used to map LrYu. Five simple sequence repeat （SSR） markers on chromosome 2BS were found closely linked to LrYu. Among these markers, Xwmc770 is the most closely linked, with a genetic distance of 5.7 cM.