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study of the effect of atorvastatin on the interaction between ICAM-1 and CD11b by live-cell single-molecule force spectroscopy

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science china Chemistry 2010年第4期53卷 752-758页

作者： li yi1, shi xiaoli2, liu huiliang1,{3., the general hospital of chinese people’s armed police forces, Beijing 10003., china 2Beijing National Laboratory for Molecular sciences Institute of Chemistry, chinese Academy of sciences, Beijing 100190, china 3.tate Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology, Beijing 100071, china 1. department of cardiology the General Hospital of Chinese People’s Armed Police Forces Beijing 100039 China2. Beijing National Laboratory for Molecular Sciences Chinese Academy of Sciences Beijing 100190 China4. Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China3. State Key Laboratory of Pathogen and Biosecurity Beijing 100071 China5. Beijing Institute of Microbiology and Epidemiology Beijing 100071 China

The interaction between the cell adhesion molecule CD11b and its ligand ICAM-1 plays an important role in inflammatory responses in the disease of atherosclerosis. Atorvastatin is a commonly prescribed statin drug which has been considered as one of the most potent therapeutic agents for atherosclerosis due to its lipid-lowering effect. Recently, there is a growing body of evidence that atorvastatin has anti-inflammatory effect. We have applied the advanced method of live-cell single-molecule force spectroscopy to investigate the effect of atorvastatin on adhesion force between ICAM-1 and CD11b. Our result showed that single-molecule binding force of ICAM-1 and CD11b detected by AFM in the living cells was about 40 pN, and atorvastatin did not affect this force by blocking ICAM-1 or CD11b. This was different from the ICAM-1 monoclonal antibody, which could directly reduce the binding force of ICAM-1 and CD11b. Flow cytometry results revealed that atorvastatin pretreatment decreased the ICAM-1 expression in TNF-α activated HUVECs, which may contribute to its anti-inflammatory effect. The study provides a new approach to study anti-inflammatory mechanism for clinic drugs.

关键词： atherosclerosis atorvastatin atomic force microscopy single-molecule force spectroscopy protein-protein interaction

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The effect of cigarette smoke extract on thrombomodulinthrombin binding: an atomic force microscopy study

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science china(life sciences) 2012年第10期55卷 891-897页

作者： WEI YuJie zhang xuejie XU li {3. shaoqiong li {3. fang {3. liu huiliang department of cardiology the General Hospital of Chinese People's Armed Police ForcesBeijing 100039China Beijing National Laboratory for Molecular sciences CAS Key Laboratory of Molecular Nanostructure and Nanotechnology Institute of ChemistryChinese Academy of SciencesBeifing 100190China Beijing Institute of Biotechnology Academy of Military Medical SciencesBeijing 100071China

Cigarette smoking is a well-known risk factor for cardiovascular disease. smoking can cause vascular endothelial dysfunction and consequently trigger haemostatic activation and thrombosis. However, the mechanism of how smoking promotes thrombosis is not fully understood. Thrombosis is associated with the imbalance of the coagulant system due to endothelial dysfunction. As a vital anticoagulation cofactor, thrombomodulin (TM) located on the endothelial cell surface is able to regulate intravascular coagulation by binding to thrombin, and the binding results in thrombosis inhibition. This work focused on the effects of cigarette smoke extract (CsE) on TM-thrombin binding by atomic force microscopy (AFM) based single-molecule force spectroscopy. The results from both in vitro and live-cell experiments indicated that CsE could notably reduce the binding probability of TM and thrombin. This study provided a new approach and new evidence for studying the mechanism of thrombosis triggered by cigarette smoking.

关键词： cigarette smoke extract (CsE) thrombin thrombomodulin AFM single-molecule force spectroscopy protein-proteininteraction

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chinese expert consensus on the non-invasive imaging examination pathways of stable coronary artery disease

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Journal of Geriatric cardiology 2018年第1期15卷 30-40页

作者： Yun-Dai CHEN Wei-yi fang Ji-Yan CHEN Zhan-Ming FAN Chuan-Yu GAO Jun-Bo GE Zuo-Xiang HE Yong HUO Lang li si-Jin li Xi-lie LU Bin LV Ju-ying QIAN Ya-Jun shi Zhu-Jun sHEN Jing WANG yi-Ning WANG Lei XU li YANG Bo YU Mei zhang Jun-Jie YANG shu-Yang zhang Xiao-li zhang shi-Hua ZHAO Yang ZHENG Yu-Chi HAN Guang ZHI chinese PLA general hospital Beijing China shanghai Chest hospital Shanghai Jiaotong University Shanghai China Guangdong general hospital Guangzhou China Beijing Anzhen hospital Capital Medical University Beijing China Henan Provincial people's hospital Zhengzhou China Zhongshan hospital Fudan University Shanghai China Fuwai hospital Chinese Academy of Medical Sciences Beijing China The First hospital of Peking University Beijing China The First Affiliated hospital of Guangxi Medical University Nanning China The First Affiliated hospital of shanxi Medical University Taiyuan China Peking Union Medical College hospital Beijing China The 2^nd Affiliated hospital of Harbin Medical University Harbin China Qilu hospital of shandong University Jinan China The First Bethune hospital of Jilin University Changchun China University of Pennsylvania United States

1 Introduction Early detection and diagnosis of stable coronary artery disease （sCAD） is essential for proactive secondary prevention of myocardial infarction （MI）, control of disease progress, and reduction of mortality. Clinical decision-making in modem medicine is increasingly dependent on cardiovascular imaging techniques. 2012 ACCF/AHA/ACP/AATs/ PCNA/sCAI/sTs guideline for the diagnosis and management of patients with stable ischemic heart disease has been issued by American Heart Association （AHA）. European society of {3. （EsC） has issued 2013.EsC guidelines on the management of stable coronary artery disease.

关键词： Expert consensus Clinical pathway Coronary artery disease Non-invasive imaging examination Pre-test probabilities

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应用新型介入瓣膜行经导管主动脉瓣置入术的实验研究

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中华心血管病杂志 2011年第11期39卷 1005-1010页

作者：王建铭杨剑杨丽芳张学昕胡运刘金城俞世强易定华第四军医大学西京医院心血管外科西安710032 第四军医大学西京医院麻醉科西安710032 第四军医大学西京医院放射影像科西安710032 第四军医大学西京医院超声科西安710032

目的探讨应用新型介入瓣膜在实验动物体内行经导管主动脉瓣置入术（TAVI）的可行性，并观察新型介入瓣膜置入后的短期效果。方法选取健康成年绵羊20只，在全身麻醉及成像造影设备的辅助下，经绵羊一侧颈总动脉置入输送鞘管，于主动脉瓣... 详细信息

目的探讨应用新型介入瓣膜在实验动物体内行经导管主动脉瓣置入术（TAVI）的可行性，并观察新型介入瓣膜置入后的短期效果。方法选取健康成年绵羊20只，在全身麻醉及成像造影设备的辅助下，经绵羊一侧颈总动脉置入输送鞘管，于主动脉瓣环处释放新型介入瓣膜。以左心室和主动脉造影及经胸超声心动图观察介入瓣膜的位置和作用。记录术后3.d实验动物的存活情况。结果所有实验绵羊均置入新型介入瓣膜。15只（75％）存活绵羊的术后造影显示介入瓣膜位置良好，无移位，未影响二尖瓣；冠状动脉开口显影良好，冠状动脉通畅。经胸超声心动图显示5例绵羊存在轻度瓣周漏。术后存活绵羊主动脉瓣反流百分比由术前（1．25±0．46）％增加至（4．52±3.56）％（P〈0．05）。术后主动脉瓣有效瓣口面积、主动脉收缩压、主动脉舒张压、平均主动脉压、左心室收缩压、左心室舒张末压、心率与术前比较差异均无统计学意义（P〉0．05）。5只（25％）绵羊于TAVI后3.d内死亡，其中介入瓣膜释放展开后即刻，因左冠状动脉开口堵塞发生心室颤动而死亡1只，术后20min死于急性心肌梗死1只，术后8和12h死于二尖瓣反流2只，术后26d死于感染性心内膜炎1只。结论采用新型介入瓣膜在实验动物体内行TAVI是可行的。短期内安全、有效。

关键词：心脏导管插入术心脏瓣膜人工主动脉瓣

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2型糖尿病合并冠心病患者血清miR-21、miR-155水平与脂代谢的关系

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心血管康复医学杂志 2019年第4期28卷 419-423页

作者：石一夫张焱王奕夏芳梁洁韩荣丽薛红梅宋光明胡延军上海市徐汇区大华医院心内科上海市200237 上海市徐汇区大华医院内分泌科上海市200237 上海市第六人民医院老年科

目的:探讨T2DM)合并CHD患者血清miR-21、miR-155水平与脂代谢的关系。方法: 2016年~2017年我院收治的13.例T2DM患者,根据是否合并CHD,患者被分为T2DM合并CHD组(T2DM+CHD组,60例)和单纯T2DM组(74例),检测两组血糖、血脂指标,及血清miR-21... 详细信息

目的:探讨T2DM)合并CHD患者血清miR-21、miR-155水平与脂代谢的关系。方法: 2016年~2017年我院收治的13.例T2DM患者,根据是否合并CHD,患者被分为T2DM合并CHD组(T2DM+CHD组,60例)和单纯T2DM组(74例),检测两组血糖、血脂指标,及血清miR-21、miR-155水平,并进行比较。结果:两组患者一般资料、血压、人体质量指数(BMI)、血糖、总胆固醇(TC)水平差异均无显著性( P 均>0.05)。与单纯T2DM组比较,T2DM+CHD组甘油三酯(TG)[(1.89±0.92) mmol/L比(2.75±1.61)mmol/L]、低密度脂蛋白胆固醇(LDL-C)[(2.83.0.79) mmol/L比(3.52±1.24) mmol/L]、血清miR-21[(0.93.0.15)比(1.86±0.24)]水平显著升高,高密度脂蛋白胆固醇(HDL-C)[(1.3.±0.3.)mmol/L比(0.94±0.3.)mmol/L]、血清miR-155[(0.95±0.19)比(0.27±0.10)]水平显著降低, P 均=0.001。多因素Logistic回归分析显示,TG、LDL-C、miR-21是T2DM合并CHD发生的独立危险因素(OR=2.800~4.986, P 均155则是其独立保护因素(OR=0.3.4,0.3.7, P 均son相关性分析显示,血清miR-21水平与TG、LDL-C水平呈显著正相关( r =0.415,0.506, P 1),血清miR-155水平与TG、LDL-C水平呈显著负相关( r =-0.3.7,-0.526, P 1)。结论:在T2DM合并CHD患者,血清miR-21与TG、LDL-C水平呈显著正相关,而miR-155与TG、LDL-C水平呈显著负相关。

关键词：糖尿病,2型冠心病微RNAs

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