The effect of cigarette smoke extract on thrombomodulinthrombin binding: an atomic force microscopy study

作     者：WEI YuJie ZHANG XueJie XU Li YI ShaoQiong LI Yi FANG XiaoHong LIU HuiLiang 

作者机构：Department of Cardiologythe General Hospital of Chinese People's Armed Police ForcesBeijing 100039China Beijing National Laboratory for Molecular SciencesCAS Key Laboratory of Molecular Nanostructure and Nanotechnology Institute of ChemistryChinese Academy of SciencesBeifing 100190China Beijing Institute of BiotechnologyAcademy of Military Medical SciencesBeijing 100071China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2012年第55卷第10期

页      面：891-897页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0803[工学-光学工程] 

基　　金：supported by the National Basic Research Program of China(Grant No.2011CB911001) National Natural Science Foundation of China(Grant Nos.21127901 and 21121063) Chinese Academy of Sciences 

主　　题：cigarette smoke extract (CSE) thrombin thrombomodulin AFM single-molecule force spectroscopy protein-proteininteraction 

摘      要：Cigarette smoking is a well-known risk factor for cardiovascular disease. Smoking can cause vascular endothelial dysfunction and consequently trigger haemostatic activation and thrombosis. However, the mechanism of how smoking promotes thrombosis is not fully understood. Thrombosis is associated with the imbalance of the coagulant system due to endothelial dysfunction. As a vital anticoagulation cofactor, thrombomodulin (TM) located on the endothelial cell surface is able to regulate intravascular coagulation by binding to thrombin, and the binding results in thrombosis inhibition. This work focused on the effects of cigarette smoke extract (CSE) on TM-thrombin binding by atomic force microscopy (AFM) based single-molecule force spectroscopy. The results from both in vitro and live-cell experiments indicated that CSE could notably reduce the binding probability of TM and thrombin. This study provided a new approach and new evidence for studying the mechanism of thrombosis triggered by cigarette smoking.