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Genome-Wide Analysis of the KANADI Gene Family and Its Expression Patterns under Different Nitrogen Concentrations Treatments in Populus trichocarpa

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Phyton-International Journal of Experimental Botany 2023年第7期92卷 2001-2015页

作者： Minghui Niu Heng Zhang xiangyang li Zhibao Hu Hongjiao Zhang Zhiru Xu Chunpu Qu Guanjun liu State Key Laboratory of Tree Genetics and Breeding(Northeast Forestry University) School of ForestryNortheast Forestry UniversityHarbin150040China Key Laboratory of Saline-Alkali Vegetation Ecology Restoration(Northeast Forestry University) Ministry of EducationHarbin150040China College of life Science Northeast Forestry UniversityHarbin150040China College of Forestry Guizhou UniversityGuiyang550025China

KANADI(KAN)is a plant-specific gene that controlled the polarity development of lateral *** mainly acted on the abaxial characteristics of plants to make the lateral organs ***,it had been less identified in woody *** this study,the members of the KAN gene family in Populus trichocarpa were identified and analyzed using the bioinformatics *** results showed that a total of 8 KAN family members were screened out,and each member contained the unique GARP domain and conserved region of the family *** analysis and their gene structures revealed that all KAN genes from ***,Arabidopsis thaliana,and Nicotiana benthamiana could be divided into four subgroups,while the eight genes in *** were classified into three subgroups,*** analysis of tissue-specific expression indicated that PtKAN1 was highly expressed in young leaves,PtKAN6 was highly expressed in young leaves and mature leaves,PtKAN2,PtKAN5,and PtKAN7 were highly expressed in nodes and internodes,PtKAN8 was highly expressed in roots,and PtKAN3 and PtKAN4 showed low expression levels in all *** them,PtKAN2 and PtKAN6,and PtKAN4 and PtKAN5 might have functional *** high nitrogen concentrations,PtKAN2 and PtKAN8 were highly expressed in mature stems and leaves,respectively,while PtKAN4,PtKAN5,and PtKAN7 were highly expressed in *** study laid a theoretical foundation for further study of the KAN genemediated nitrogen effect on root development.

关键词： Bioinformatics analysis KANADI gene family nitrogen Populus trichocarpa

Enhancing prime editing efficiency by modified pegRNA with RNA G-quadruplexes

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Journal of Molecular Cell Biology 2022年第4期14卷 65-68页

作者： xiangyang li Xin Wang Wenjun Sun hisheng Huang Mingtian Zhong Yuan Yao Quanjiang Ji Xingxu Huang Gene Editing Center School of Life Science and TechnologyShanghai Tech UniversityShanghai 201210China University of Chinese Academy of Sciences Beijing 100049China Institute for Brain Research and Rehabilitation Guangdong Key Laboratory of Mental Health and Cognitive ScienceCenter forStudies of Psychological ApplicationSouth China Normal UniversityGuangzhou 510631China HangzhouGlobal Scientific and Technological Innovation Center Zhejiang UniversityHangzhou 311215China Schoolof Physical Science and Technology.ShanghaiTech University Shanghai 201210China Guangzhou Laboratory Guangzhou International Bio IslandGuangzhou 510005China

Dear Editor,Recent study shows that the prime editing system fusing the Cas9 nickase and reverse transcriptase could perform all types ofgene modifications,including basesubstitutions(transitionsand transversions),small insertions,and deletions,without requiringdonor DNA or double-strand breaks(DSBs)(Anzalone et al.,2019).Despite the accuracy and versatility,the efficiencyof the prime editor(PE)is often insufficient,which limits its broadapplications.

关键词： prime donor transitions

Novel thin plate element theory based on a continuity re-relaxed technique

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Science China(Technological Sciences) 2010年第9期53卷 2450-2457页

作者： CUI xiangyang,li GuangYao & ZHENG Gang State Key Laboratory of Advanced Design and Manufacturing for Vehicle Body,Hunan University,Changsha 410082,China 1. State Key Laboratory of Advanced Design and Manufacturing for Vehicle Body Hunan University Changsha 410082 China

Thin plate problem has been receiving much interest due to its wide application in *** this paper,a novel thin plate element theory is proposed based on a continuity re-relaxed technique to avoid the high continuity requirement for thin plate formulation which needs the high computation *** problem is first discretized into a set of background cells with field nodes,and only deflection field is treated as the field *** top of the background cells,the integration domains are further *** curvatures over integration domains are restructured through the divergence theorem,and the continuity requirement of the trial deflection function for thin plate problems can be *** Galerkin weak form is then used to create the discretized system *** curvatures in the integration domains are constant,and the stiffness matrix of the system can be computed directly without numerical *** rotational essential boundary conditions are imposed in the process of curvature field *** numerical examples are computed using the RPIM approximation *** excellent results demonstrate the efficiency of the proposed method.

关键词： thin plate problem continuity re-relaxed curvature field restructured Galerkin weak form

Asymmetric construction of axial and planar chirality with N-heterocyclic carbene(NHC) organocatalysis

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Science China Chemistry 2024年

作者： Meng Zhang Xiaoqun Yang Xiaolin Peng xiangyang li Zhichao Jin State Key Laboratory of Green Pesticide Guizhou University

N-heterocyclic carbene(NHC) organocatalytic reactions that have been developed for asymmetric construction of axial and planar chirality are systematically summarized and discussed in this review. The challenges and limitations inherent to this highly reactive research field are highlighted towards the end of the review. Additionally, based on our understanding of the current advancements in NHC organocatalysis, we propose potential future directions for further exploration.

关键词：

Gene editing and its applications in biomedicine

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Science China(life Sciences) 2022年第4期65卷 660-700页

作者： Guanglei li xiangyang li Songkuan Zhuang liren Wang Yifan Zhu Yangcan Chen Wen Sun Zeguang Wu Zhuo Zhou Jia Chen Xingxu Huang Jin Wang Dali li Wei li Haoyi Wang Wensheng Wei School of life Science and Technology ShanghaiTech UniversityShanghai201210China Department of Clinical Laboratory Shenzhen Institute of Translational MedicineThe First Affiliated Hospital of Shenzhen UniversityShenzhen Second People’s HospitalShenzhen518035China Shanghai Frontiers Science Research Base of Genome Editing and Cell Therapy Shanghai Key Laboratory of Regulatory BiologySchool of Life SciencesEast China Normal UniversityShanghai200241China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing100101China University of Chinese Academy of Sciences Beijing100049China Institute for Stem Cell and Regenerative Medicine Chinese Academy of SciencesBeijing100101China Biomedical Pioneering Innovation Center Beijing Advanced Innovation Center for GenomicsPeking-Tsinghua Center for Life SciencesPeking University Genome Editing Research CenterState Key Laboratory of Protein and Plant Gene ResearchSchool of Life SciencesPeking UniversityBeijing100871China Gene Editing Center School of Life Science and TechnologyShanghaiTech UniversityShanghai201210China Bejing Institute for Stem Cell and Regenerative Medicine Beijing100101China HIT Center for life Sciences Harbin Institute of TechnologyHarbin150001China

The steady progress in genome editing, especially genome editing based on the use of clustered regularly interspaced short palindromic repeats(CRISPR) and programmable nucleases to make precise modifications to genetic material, has provided enormous opportunities to advance biomedical research and promote human health. The application of these technologies in basic biomedical research has yielded significant advances in identifying and studying key molecular targets relevant to human diseases and their treatment. The clinical translation of genome editing techniques offers unprecedented biomedical engineering capabilities in the diagnosis, prevention, and treatment of disease or disability. Here, we provide a general summary of emerging biomedical applications of genome editing, including open challenges. We also summarize the tools of genome editing and the insights derived from their applications, hoping to accelerate new discoveries and therapies in biomedicine.

关键词： gene editing CRISPR high-throughput functional genomics diagnostics animal model therapeutics

Approaches to the Resolution of Terrestrial and Maritime Territorial Disputes

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Contemporary International Relations 2012年第5期22卷 60-62页

作者： li xiangyang li Hongli Chinese Academy of Social Sciences

Countries which have territorial disputes with China are growing increasingly worried about losing leverage against the rising giant of the *** major powers are adjusting their strategies in Asia in response to China... 详细信息

关键词：海上陆地纠纷杠杆作用领土争端中国

Charge state modulation on boron site by carbon and nitrogen localized bonding microenvironment for two-electron electrocatalytic H_(2)O_(2)production

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Chinese Chemical Letters 2023年第5期34卷 598-603页

作者： Tingting Zhang Yin Wang xiangyang li Quan Zhuang Zixuan Zhang Hong Zhou Qin Ding Yingqi Wang Yuxin Dang limei Duan Jinghai liu Inner Mongolia Key Laboratory of Carbon Nanomaterials College of Chemistry and Materials ScienceNano Innovation InstituteInner Mongolia Minzu UniversityTongliao 028000China Tongliao Center for Disease Control and Prevention Tongliao 028000China

Design of electrochemical active boron(B)site at solid materials to understand the relationships between the localized structure,charge state at the B site and electrocatalytic activity plays a crucial role in boosting the green electrochemical synthesis of hydrogen peroxide(H_(2)O_(2))via two-electron oxygen reduction(2eORR)***,we demonstrate a carbon(C)and nitrogen(N)localized bonding microenvironment to modulate the charge state of B site at the boron-carbon nitride solid(BCNs)to realize the efficient selective electrocatalytic H_(2)O_(2)*** localized chemical structure of N-B-N,N-B-C and C-B-C bonds at B site can be regulated through solid-state reaction between boron nitride(BN)and porous carbon(C)at variable *** optimized BCN-1100 achieves an outstanding H_(2)O_(2)selectivity of 89%and electron transfer number of 2.2(at 0.55 V ***),with the production of 10.55mmol/L during 2.5 h and the catalytic stability duration for 15000 *** first-principles calculations identified the dependency of localized bonding microenvironment on the OOH~*adsorption energies and relevant charge states at the boron *** localized structure of B site with BNC_(2)-Gr configuration is predicted to be the highest 2eORR activity.

关键词： Solid boron site Charge state modulation Localized bonding microenvironment Two-electron oxygen reduction H_(2)O_(2)electrosynthesis

Enlarged DNA unwinding by Nme2Cas9 permits a broadened base editing window beyond the protospacer

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Science China(life Sciences) 2024年第2期67卷 424-427页

作者： Ziting Chen xiangyang li Qian Zhang Wenjun Sun Xiaoxuan Song Xia Zhang Xingxu Huang Bo Sun School of life Science and Technology and Gene Editing Center ShanghaiTech UniversityShanghai 201210China Zhejiang Lab HangzhouZhejiang 311121China CAS Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China

Dear Editor,Base editing has been developed to be one of the effective CRISPR-based gene editing techniques(Rees and liu,2018).Base editors(BEs)take advantage of DNAmodifying enzymes fused with a catalytically impaired CRISPR protein to install base *** BE,DNA-modifying enzymes often process nontarget singlestranded DNA(NTS)generated by Cas proteins.

关键词： install base winding

Transcription-wide impact by RESCUE-induced off-target single-nucleotide variants in mammalian cells

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Journal of Molecular Cell Biology 2023年第2期15卷 17-28页

作者： Guo li Xiaoning Zhu Yihan Wang Hongru Ma Yuzhe Wang Hanyu Wu xiangyang li Yiling Wang Jianen Gao Xuexin Chen Xingxu Huang Yuan Yao Xiaoxiang Hu College of Chemical and Biological Engineering Zhejiang UniversityHangzhou 310027China ZJU-Hangzhou Global Scientific and Technological Innovation Center Zhejiang UniversityHangzhou 311200China State Key Laboratory of Agrobiotechnology College of Biological SciencesChina Agricultural UniversityBeĳing 100193China National Research Institute for Family Planning Beĳing 100081China School of life Science and Technology ShanghaiTech UniversityShanghai 201210China Institute of Insect Sciences College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China Zhejiang Lab Hangzhou 311121China

RNA base editing is a promising tool in precise molecular therapy. Currently, there are two widely used RNA base editors, REPAIRand RESCUE. REPAIR only facilitates A-to-I conversions, while RESCUE performs both A-to-I and C-to-U conversions. Thus, RESCUEcan generate twice the number of mutations compared to REPAIR. However, transcription-wide impact due to RESCUE-induced offtarget single-nucleotide variants (SNVs) is not fully appreciated. Therefore, to determine the off-target effects of RESCUE-mediatedediting, we employed transcription-wide sequencing on cells edited by RESCUE. The SNVs showed different off-target effects onmRNA, circRNA, lncRNA, and miRNA expression patterns and their interacting networks. Our results illustrate the transcriptionwide impact of RESCUE-induced off-target SNVs and highlight the need for careful characterization of the off-target impact by thisediting platform.

关键词： transcription-wide off-target SNV RESCUE RNA