Enhancing prime editing efficiency by modified pegRNA with RNA G-quadruplexes

作     者：Xiangyang Li Xin Wang Wenjun Sun hisheng Huang Mingtian Zhong Yuan Yao Quanjiang Ji Xingxu Huang Xiangyang Li;Xin Wang;Wenjun Sun;Shisheng Huang;Mingtian Zhong;Yuan Yao;Quanjiang Ji;Xingxu Huang

作者机构：Gene Editing CenterSchool of Life Science and TechnologyShanghai Tech UniversityShanghai 201210China University of Chinese Academy of SciencesBeijing 100049China Institute for Brain Research and RehabilitationGuangdong Key Laboratory of Mental Health and Cognitive ScienceCenter forStudies of Psychological ApplicationSouth China Normal UniversityGuangzhou 510631China HangzhouGlobal Scientific and Technological Innovation CenterZhejiang UniversityHangzhou 311215China Schoolof Physical Science and Technology.ShanghaiTech UniversityShanghai 201210China Guangzhou LaboratoryGuangzhou International Bio IslandGuangzhou 510005China 

出 版 物：《Journal of Molecular Cell Biology》 (分子细胞生物学报（英文版）)

年 卷 期：2022年第14卷第4期

页      面：65-68页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：supported by the Emergency Key Program of Guangzhou Laboratory(EKPG21-18) the Leading Talents of Guangdong Province Program(608285568031) 

主　　题：prime donor transitions 

摘      要：Dear Editor,Recent study shows that the prime editing system fusing the Cas9 nickase and reverse transcriptase could perform all types ofgene modifications,including basesubstitutions(transitionsand transversions),small insertions,and deletions,without requiringdonor DNA or double-strand breaks(DSBs)(Anzalone et al.,2019).Despite the accuracy and versatility,the efficiencyof the prime editor(PE)is often insufficient,which limits its broadapplications.