Enlarged DNA unwinding by Nme2Cas9 permits a broadened base editing window beyond the protospacer

作     者：Ziting Chen Xiangyang Li Qian Zhang Wenjun Sun Xiaoxuan Song Xia Zhang Xingxu Huang Bo Sun 

作者机构：School of Life Science and Technology and Gene Editing CenterShanghaiTech UniversityShanghai 201210China Zhejiang LabHangzhouZhejiang 311121China CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2024年第67卷第2期

页      面：424-427页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the National Natural Science Foundation of China(32022048,32271505) the Natural Science Foundation of Shanghai(22ZR1441900,23ZR1442200) the Key Research Project of Zhejiang Laboratory 

主　　题：install base winding 

摘      要：Dear Editor,Base editing has been developed to be one of the effective CRISPR-based gene editing techniques(Rees and Liu,2018).Base editors(BEs)take advantage of DNAmodifying enzymes fused with a catalytically impaired CRISPR protein to install base *** BE,DNA-modifying enzymes often process nontarget singlestranded DNA(NTS)generated by Cas proteins.