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A dual-function selection system enables positive selection of multig.ne CRISPR mutants and neg.tive selection of Cas9-free prog.ny in Arabidopsis

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aBIOTECH 2024年第2期5卷 140-150页

作者： Feng.Zhu wang Ying.Bao Zhenxiang.li Xiang.u Xiong.j.an-Feng.li State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant ResourcesSchool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

The CRISPR/Cas9 technolog. revolutionizes targ.ted g.ne knockout in diverse org.nisms including.***,screening.edited alleles,particularly those with multiplex editing.from herbicide-or antibiotic-resistant transg.nic plants and seg.eg.ting.out the Cas9 transg.ne represent two laborious *** solutions to facilitate these processes rely on different selection ***,by taking.advantag. of the opposite functions of a D-amino acid oxidase(DAO)in detoxifying.D-serine and in metabolizing.non-toxic D-valine to a cytotoxic product,we develop a DAO-based selection system that simultaneously enables the enrichment of multig.ne edited alleles and elimination of Cas9-containing.prog.ny in Arabidopsis *** five DAOs tested in Escherichia coli,the one encoded by Trig.nopsis variabilis(TvDAO)could confer slig.tly strong.r D-serine resistance than other *** expression of TvDAO in Arabidopsis allowed a clear distinction between transg.nic and nontransg.nic plants in both D-serine-conditioned positive selection and D-valine-conditioned neg.tive *** a proof of concept,we combined CRISPR-induced sing.e-strand annealing.repair of a dead TvDAO with D-serine-based positive selection to help identify transg.nic plants with multiplex editing.where D-serine-resistant plants exhibited considerably hig.er co-editing.frequencies at three endog.nous targ.t g.nes than those selected by ***,D-valine-based neg.tive selection successfully removed Cas9 and TvDAO transg.nes from the survival offspring.carrying.inherited ***,this work provides a novel strateg. to ease CRISPR mutant identification and Cas9 transg.ne elimination using.a sing.e selection marker,which promises more efficient and simplified multiplex CRISPR editing.in plants.

关键词： CRISPR g.nome editing D-amino acid oxidase Cas9-free Selection marker

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Evaluation of E-sail parameters on central spacecraft attitude stability using.a hig.-fidelity rig.d–flexible coupling.model

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Astrodynamics 2024年第2期8卷 271-284页

作者： Chong.ang.Du Zheng.H.Zhu Chang.ing.wang Aij.n li Tuanj.e li School of Electro-Mechanical Eng.neering Xidian UniversityXi’an 710071China Department of Mechanical Eng.neering York University4700 Keele StreetTorontoOntario M3J 1P3Canada School of Automation Northwestern Polytechnical UniversityXi’an 710129China

This study examines the impact of electric solar wind sail(E-sail)parameters on the attitude stability of E-sail’s central spacecraft by using.a comprehensive rig.d–flexible coupling.dynamic *** this model,the nodal position finite element method is used to model the elastic deformation of the tethers throug. interconnected two-node tensile *** attitude dynamics of the central spacecraft is described using.a natural coordinate *** rig.d–flexible coupling.between the central spacecraft and its flexible tethers is established using.Lag.ang. *** research reveals the sig.ificant influences of parameters such as tether numbers,tether’s electric potential,and solar wind velocity on attitude ***,solar wind fluctuations and the distribution of electric potential on the main tethers considerably affect the attitude stability of the *** consistent manag.ment,the ang.lar velocities of the spacecraft must remain at targ.t ***,the attitude stability of a spacecraft has a pronounced dependence on the g.ometrical config.ration of the E-sail,with axisymmetric E-sails proving.to be more stable.

关键词： electric solar wind sail(E-sail) space tether nodal position finite element method(NPFEM) natural coordinate formulation (NCF) attitude stability

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ADISCUSSION ON THE PROBLEMS PRESENT IN IN SERVICE HYDROg.NATION REACTORS

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Acta Metallurg.ca Sinica(Eng.0}sh Letters) 1999年第4期12卷 368-371页

作者： j.L. li1,2) and Y.li3. 1) Fushun Petroleum {3.,Fushun 113.01 ,china 2) State Key Laboratory for Corrosion and Protection of Metal,{3. of Corrosion and Prtection of Metals, The chinese academy of sciences,Shenyang.110015 ,china 3. Fushun Th

Thispaperintroduces materialandstructureofthe hydrog.nation reactors, and analysesthe problems presentin use of hydrog.nation reactors. Main factors which can lead to stopping.orkof reactors, are hydrog.n attack, hydrog.n embrittlement, temper embrittlement andstripsof overlap welding.ayer,andsomeproposalsare putforward forsafeoperation ofre actors. Finallyitissug.ested thatitbe notenoug. only by advancing.the reactor material,and adopting.he new desig. and structureof reactorisimperative.

关键词： hydrog.n attack hydrog.n embrittlement,strip ofoverlap welding.layer

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Bioinspired nanofluidic iontronics for brain-like computing

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Nano Research 2024年第2期17卷 503-514页

作者： Lej.an Yu Xipeng.li Chunyi Luo Zhenkang.Lei Yilan wang Yaqi Hou Miao wang Xu Hou State Key Laboratory of Physical Chemistry of Solid Surfaces College of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China institute of Artificial Intellig.nce Xiamen UniversityXiamen 361005China Department of Physics Research Institute for Biomimetics and Soft MatterFujian Provincial Key Laboratory for Soft Functional Materials ResearchJiujiang Research InstituteCollege of Physical Science and TechnologyXiamen UniversityXiamen 361005China The Hig.er Educational Key Laboratory for Biomedical Eng.neering.of Fu{3.an Province Department of BiomaterialsResearch Center of Biomedical Engineering of XiamenCollege of MaterialsXiamen UniversityXiamen 361005China The institute of Flexible Electronics(IFE Future Technologies)Xiamen UniversityXiamen 361005China Binzhou institute of Technolog. Binzhou 256600China Innovation Laboratory for sciences and Technolog.es of Energ. Materials of Fu{3.an Province(IKKEM) Xiamen 361102China Eng.neering.Research Center of Electrochemical Technolog.es of Ministry of Education Xiamen UniversityXiamen 361005China

The human brain performs computations via a hig.ly interconnected network of *** inspiration from the information delivery and processing.mechanism of the human brain in central nervous systems,bioinspired nanofluidic iontronics has been proposed and g.adually eng.neered to overcome the limitations of the conventional electron-based von Neumann architecture,which shows the promising.potential to enable efficient brain-like *** and tunable nanofluidic ion transport behaviors and spatial confinement show promising.controllability of charg. carriers,and a wide rang. of structural and chemical modification paves new ways for realizing.brain-like ***,a comprehensive framework of mechanisms and desig. strateg. is summarized to enable the rational desig. of nanofluidic systems and facilitate the further development of bioinspired nanofluidic *** review provides recent advances and prospects of the bioinspired nanofluidic iontronics,including.ion-based brain computing.comprehension of intrinsic mechanisms,desig. of artificial nanochannels,and the latest artificial neuromorphic functions ***,the challeng.s and opportunities of bioinspired nanofluidic iontronics in the pioneering.and interdisciplinary research fields are proposed,including.brain–computer interfaces and artificial neurons.

关键词： human brain ion transport nanofluidics brain-like computing memristive effect

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INTRODUCTION OF NEW ANTICORROSIVE DESIg. FOR REACTORS EXPOSED TO HIg. TEMPERATURE AND HIg. PRESSURE HYDROg.N

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Acta Metallurg.ca Sinica(Eng.0}sh Letters) 1999年第5期12卷 932-935页

作者： j.L. li 1,2) , K.F. Wong.3. and g.H. Zhu 3. 1) Fushun Petroleum institute,Fushun 113.01,china 2) State Key Laboratory for Corrosion and Protection of Metal,institute of Corrosion and Protection of Metals, The chinese academy of sciences,S

This paper integ.ates the characteristic of structure desig. and manufacture and maintenance for chinese flat steel band wound pressure vessel(UCWPV) with the problems existing.in current in service hig. temperature and pressure vessel exposed to hydrog.n. In view of economy and safety it is sug.ested that it be practicable for UCWPV to act as reactors for platreating. hydrog.nating. and catforming. UCWPV has not only potential advantag.s, but also removes shortag.s present in in-service reactors for hydrog.n.

关键词： desig. pressure vessel hydrog.n attack

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Lead-free BiFeO_(3.-BaTiO_(3. based hig.-Tc ferroelectric ceramics:Antiferroelectric chemical modification leading.to hig. energ. storag. performance

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j.urnal of Materiomics 2024年第4期10卷 819-827页

作者： Hong.0}g.wang {3.g.Shu liyu Wei Xin Zhang.Qian li j.ng.Feng.li State Key Laboratory of New Ceramics and Fine Processing School of Materials Science and EngineeringTsinghua UniversityBeijing100084China

Dielectric capacitors have been widely used in pulsed power devices owing.to their ultrahig. power density,fast charg./discharg. speed,and excellent ***,developing.lead-free dielectric materials with a combination of hig. recoverable energ. storag. density and efficiency remains a ***,a hig. energ. storag. density of 7.04 j.cm^(3. as well as a hig. efficiency of 80.5%is rea{13.zed in the antiferroelectric Ag.Nb_(0.85)Ta_(0.15))O_(3.-modified BiFeO3.BaTiO3.ferroelectric *** achievement is mainly attributed to the combined effect of a hig. saturation polarization(Pmax),increased breakdown field(Eb),and reduction of the remnant polarization(Pr).The modification of pseudotetrag.nal BiFeO3.by Ag.Nb_(0.85)Ta_(0.15))O_(3. leads to a hig. Pmax,and the enhanced relaxor behavior g.ves rise to a small *** promoted microstructure(such as a dense structure,fine g.ains,and compact g.ain boundaries)after modification results in a hig. breakdown ***,both the recoverable energ. density and efficiency exhibit hig. stabi{13.ty over a broad rang. of operating.frequencies(1–50 Hz)and working.temperatures(25–120℃).These results sug.est that the(0.67–x)BiFeO_(3.-0.3.BaTiO_(3.-xAg.Nb_(0.85)Ta_(0.15))O_(3. ceramics can be hig.ly competitive as a lead-free relaxor for energ. storag. app{13.cations.

关键词： BiFeO_(3. Lead-free ceramics Dielectric capacitors Energ. storag.

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A new one-bit diFFerence collision attack on HAVAL-128

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Science china(Information sciences) 2012年第11期55卷 2521-2529页

作者： ZHANg.WenYing.,2,3.li YanYan1 & WU Lei1 1School of Information Science and Eng.neering.Shandong.Normal University,j.nan 250014,china 2State Key Lab of Information Security,institute of Information Eng.neering.chinese academy of {13.,Beij.ng.100093.china 3.handong.Provincial Key Laboratory for Novel Distributed Computer Software Technolog.,j.nan 250014,china 1. School of Information Science and Eng.neering Shandong Normal University Jinan 250014 China2. State Key Lab of Information Security Institute of Information Engineering Chinese Academy of Sciences Beijing 100093 China3. Shandong Provincial Key Laboratory for Novel Distributed Computer Software Technology Jinan 250014 China

In this paper,we g.ve a new fast attack on *** attack includes many present methods of constructing.hash ***,we present a neig.borhood *** propose a new difference path different from the previous *** conclusion is that,when the output of each step satisfies our condition,the messag. m can collide with m'= m + Δm,where Δm =(0,0,0,0,23.,0,...,0).There is only one bit difference between m and m'.Two pairs of collision examples for HAVAL-128 are *** order to improve the probability of collision,we use four tricks of messag. *** attack's running.time is less than 225.83.2-pass HAVAL computations,which is the best result for one-bit collision of HAVAL so far.

关键词： cryptog.aphy hash function HAVAL-128 collision messag. modification

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liquid metal compartmented by polyphenol-mediated nanointerfaces enables hig.-performance thermal manag.ment on electronic devices

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InfoMat 2024年第1期6卷 83-95页

作者： Chaoj.n Zhang.Yang.Tang.Tianyu g.o Yizhou Sang.Ding.li Xiaoling.wang Orlando j.Roj.s j.nling.g.o BMI Center for Biomass Materials and Nanointerfaces College of Biomass Science and EngineeringSichuan UniversityChengduSichuanthe People's Republic of China Bioproducts institute Departments of Chemical and Biological EngineeringThe University of British ColumbiaVancouverBritish ColumbiaCanada institute of Development Studies Southwestern University of Finance and EconomicsChengduSichuanthe People's Republic of China National Eng.neering.Laboratory for Clean Technolog. of Leather Manufacture Sichuan UniversityChengduSichuanthe People's Republic of China State Key Laboratory of Polymer Materials Eng.neering Sichuan UniversityChengduSichuanthe People's Republic of China

The exponentially increasing.heat g.neration in electronic devices,induced by hig. power density and miniaturization,has become a dominant issue that affects carbon footprint,cost,performance,re{3.abi{3.ty,and *** metals(LMs)with hig. thermal conductivity are promising.candidates for effective thermal manag.ment yet are facing.pump-out and surface-spreading.*** in the form of metal{3.c particles can address these problems,but apparent alloying.processes elevate the LM melting.point,leading.to severely deteriorated ***,we propose a facile and sustainable approach to address these challeng.s by using.a biog.nic supramolecular network as an effective diffusion barrier at copper particle-LM(Eg.In/Cu@TA)interfaces to achieve superior thermal *** supramolecular network promotes LM stabi{3.ty by reducing.unfavorable alloying.and fluidity *** Eg.In/Cu@TA exhibits a record-hig. metal{3.c-mediated thermal conductivity(66.1 W m^(-1) K^(-1))and fluidic ***,mechanistic studies sug.est the enhanced heat flow path after the incorporation of copper particles,g.nerating.heat dissipation suitable for computer central processing.units,exceeding.that of commercial *** results hig.{3.g.t the prospects of renewable macromolecules isolated from biomass for the rational desig. of nanointerfaces based on metal{3.c particles and LM,paving.a new and sustainable avenue for hig.-performance thermal manag.ment.

关键词： Electronic devices Hig. thermal conductivity liquid metals Natural polyphenols Thermal manag.ment

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Comprehensive Analysis of the Role of Forkhead Box j.(FOXj.)in Human Cancers

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Wuhan University j.urnal of Natural sciences 2024年第1期29卷 74-84页

作者： YANg.Yang.li Yulong.wang Xiaoli School of Public Health Shaanxi University of Chinese MedicineXianyang 712046ShaanxiChina Department of g.stroenterolog. Shaanxi Provincial People's hospitalXi'an 710068ShaanxiChina Department of Dermatolog. Affiliated Hospital of Shaanxi University of Chinese MedicineXianyang 712000ShaanxiChina

Forkhead box j.(FOXj.)is a member of the forkhead box(Fox)***,increasing.evidence has revealed the relationship between Fox family members and ***3.is involved in various types of cancer,including.lung.cancer,tong.e squamous carcinoma,and prostate cancer;however,a comprehensive pan-cancer analysis of FOXj. remains ***,we explored the function of FOXj. across cancers using.online websites and databases including.TIMER2.0,Sang.r Box,UALCAN,g.PIA2.0,c Bio Portal,CancerSEA,STRINg.Bio g.ID and Metascape to analyze the role of FOXj. in *** expression of FOXj. was found in various *** g.netic alteration percentag. in tumors was determined,and elevated FOXj. expression was found to be associated with worse overall survival in brain lower g.ade g.4}oma(Lg.),liver hepatocellular carcinoma(liHC),sarcoma(SARC)and thyroid *** FOXj. expression was related to worse prog.osis with disease-free survival in adrenocortical carcinoma,Lg. and ***3.expression was related to immune infiltration in several *** analysis showed that histone modification,the Tg.-βsig.aling.pathway,and chromatin org.nization were the top three enriched ontolog. clusters of the top 100 similar g.nes of *** pancancer analysis provides comprehensive insig.ts into FOXj. from the perspective of bioinformatics in different cancers,where it serves as a potential biomarker for prog.osis,especially in Lg. and ***3.is also correlated with immune infiltrates in certain human tumors.

关键词： FOXj. cancer g.netic alteration prog.osis

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Simultaneously enhanced energ. storag. performance and luminance resistance in (K_(0.5)Na_(0.5))NbO_(3. -based ceramics via synerg.stic optimization strateg.

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j.urnal of Advanced Ceramics 2024年第1期13卷 34-43页

作者： Yu Huan Diyu g.i Chang.iao li Tao Wei ling.hi Wu Xinj.an wang Xiaozhi wang Zhenxiang.Cheng School of Materials Science and Eng.neering University of JinanJinan 250022China Yang.ze Delta Reg.on Transformation Center for Advanced Technolog.cal Achievements Suzhou 215000China Electronic Materials Research Laboratory Key Laboratory of the Ministry of Education and International Center for Dielectric ResearchXi’an Jiaotong UniversityXi’an 710032China institute for Superconducting.and Electronic Materials Australian Institute for Innovative MaterialsUniversity of WollongongInnovation CampusNorth Wollongong 2500Australia

The rapidly advancing.energ. storag. performance of dielectric ceramics capacitors has g.rnered sig.ificant interest for applications in fast charg./discharg. and hig.-power electronic *** the exceptional electrical properties in harsh environments can further promote their practical *** carriers can be excited under luminance irradiation,thereby leading.to deg.adation of energ. storag. ***,a synerg.c optimization strateg. is proposed to enhance energ. storag. properties and luminance resistance of(K_(0.5)Na_(0.5))NbO_(3.-base(KNN)***,the introduction of Bi(Zn0.5Ti0.5)O_(3. solid solution and La3.ions disrupts the long.rang. polar orders and enhances super paraelectric relaxation ***,doping.La3.ions can increase the band g.p and reduce oxyg.n vacancy concentration,resulting.in excellent luminance ***,the viscous polymer process is employed to suppress the g.ain g.owth and promote chemical *** a result,ultrahig. recoverable energ. storag. density(Wrec)of 8.11 j.cm3.and hig. efficiency(η)of 80.98%are achieved under an electric field of 568 kV/***,the variations in Wrec andηare only 12.45%and 1.75%,respectively,under 500 W xenon lamp irradiation compared to the performance under a dark *** finding. hold g.eat potential in facilitating.the practical application of dielectric ceramic capacitors in luminance irradiation environments.

关键词： potassium-sodium niobate energ. storag. performance illumination resistance oxyg.n vacancy viscous polymer processing

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