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Study on Tibetan Chicken embryonic adaptability to chronic hypoxia by revealing differential gene expression in heart tissue

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science china(life sciences) 2009年第3期52卷 284-295页

作者： li mei & zhao chunjiang college of animal science and technology,china agricultural university,beijing 100193,china college of animal science and technology China Agricultural University Beijing China

Oxygen concentration is essential for appropriate *** can exert a significant impact on physiological alteration of the cell and *** Chicken(Gallus gallus) is a Chinese indigenous breed inhabiting in Tibetan areas,which is also a chicken breed living at high altitude for the longest time in the *** has developed an adaptive mechanism to hypoxia,which is demonstrated by that Tibetan Chicken has much higher hatchability than low-land chicken breeds in high-altitude areas of *** the present study,Tibetan Chicken fertilized full sib eggs were incubated up to Hamburger-Hamilton stage 43 under 13% and 21% oxygen concentration,*** Chicken and Dwarf Recessive White Chicken were used as control *** hearts in all of the 3 chicken breeds under hypoxic and normoxic conditions were isolated and hybridized to Genechip Chicken Genome Array to study molecular mechanisms underlying the adaptation to high altitude of Tibetan *** a result,50 transcripts highly expressed in hypoxia are screened *** up-regulated genes,some are involved in the gene ontology(GO) such as cell growth,cell difference,muscle contraction and signal ***,the expression levels of 21 transcripts are lower in hypoxia than those in *** down-regulated genes take part in cell communication,ion transport,protein amino acid phosphorylation and signal ***,gene enrichment analyses of these differential gene expressions are mainly associated with immune system response and ion channel activity in response to ***,the transcriptional expression profiles analyzed by hierarchical clustering and CPP-SOM software in all of the 3 different chicken breeds revealed that Tibetan Chicken is much closely related to Shouguang Chicken rather than Dwarf Recessive White *** addition,12 transcripts of Tibetan Chicken breed-specific expressed genes were identified,which seem to result in a more

关键词： adaptation chronic hypoxia gene chip chicken genome array Tibetan Chicken(Gallus gallus) differential gene expression

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Sequencing and alignment of mitochondrial genomes of Tibetan chicken and two lowland chicken breeds

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science china(life sciences) 2008年第1期51卷 47-51页

作者： BAO HaiGang, zhao chunjiang, li JunYing & WU ChangXin college of animal science and technology, china agricultural university, beijing 100094, china college of animal science and technology China Agricultural University Beijing 100094 China

Tibetan chicken lives in high-altitude area and has adapted well to hypoxia genetically. Shouguang chicken and Silky chicken are both lowland chicken breeds. In the present study, the complete mito-chondrial genome sequences of the three chicken breeds were all sequenced. The results showed that the mitochondrial DNAs (mtDNAs) of Shouguang chicken and Silky chicken consist of 16784 bp and 16785 bp respectively, and Tibetan chicken mitochondrial genome varies from 16784 bp to 16786 bp. After sequence analysis, 120 mutations, including 4 single nucleotide polymorphisms (SNPs) in tRNA genes, 9 SNPs and 1 insertion in rRNA genes, 38 SNPs and 1 deletion in D-LOOP, 66 SNPs in pro-tein-coding genes, were found. This work will provide clues for the future study on the association between mitochondrial genes and the adaptation to hypoxia.

关键词： Tibetan chicken lowland chicken mitochondrial genome hypoxia

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ANALYSIS AND MONITORING FOR EPIDEMIC SYSTEM AS A BASIS FOR CUCUMBER DOWNY MILDEW WARNING IN GREENHOUSE

ANALYSIS AND MONITORING FOR EPIDEMIC SYSTEM AS A BASIS FOR C...

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第四届智能化农业信息技术国际学术会议

作者： chunjiang zhao~~(1 2*) Ming li~(1 3) Xiao Han~(1 2) Zheng Zhang~(1 2) Yan’an Wang~4 1 National Engineering Research Center for Information technology in Agriculture beijing 100097 PR china 2 Key Laboratory for Information Technologies in Agriculture the Ministry of Agriculture beijing 100097 PR china 3 college of Information and Electrical Engineering china agricultural university beijing 100083 PR china 4 college of life science Shandong agricultural university Taian 271018 PR china

＜正＞Plant disease warning is a relative new concept and method system for integrated pest management. The basic of plant disease warning is epidemiology mechanistic and monitoring *** analysis of cucumber downy mildew epidemic has been proposed as the framework and criteria of early warning ***,a wide range of monitoring methods,including computer aids,for epidemic system provide foundation of establishing, operation and evaluation for early warning models. These results and approaches would prove useful in modeling and information system development for cucumber downy mildew warning.

关键词： Cucumber downy mildew greenhouse plant epidemiology monitoring epidemics waning

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昌黎县水资源问题及其可持续利用研究

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水土保持研究 2007年第5期14卷 279-281页

作者：赵会芝任树梅河北科技师范学院园艺园林系河北昌黎066600 中国农业大学水利与土木工程学院北京100083

介绍了昌黎县的自然、地理及水资源概况,分析了昌黎县水资源开发利用中存在的问题,并提出了促进水资源可持续开发利用的具体策略,即：节约用水、进一步退耕还林、提高区域供水能力及加强管理与保护。

关键词：昌黎县水资源可持续利用

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The ciliate protozoan Colpoda cucullus can improve maize growth by transporting soil phosphates

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Journal of Integrative Agriculture 2022年第3期21卷 855-861页

作者： ZHANG Wen-li liN Qi-mei li Gui-tong zhao Xiao-rong college of Land science and technology China Agricultural UniversityBeijing 100193P.R.China

little is known regarding the ability of protozoans to transfer phosphates and improve maize *** objective of this study was to determine whether Colpoda cucullus could improve the maize phosphorus(P) level by transferring *** this three-compartment root box study,the soil in the outer compartment was inoculated with the common ciliate,***,together with the addition of either KH_(2)^(32)PO_(4),rock phosphate(RP),super phosphate(SP) or ammonium phosphate(AP),and then maize was grown in the inner *** results showed that the maize plants grown in the soil inoculated with *** had much higher ^(32)P radioactivity than the *** cucullus inoculation resulted in significant increases in dry matter by up to 25.07%,and nitrogen(N),P and potassium(K) absorption by 1–36%(Pliate inoculation(P<0.05).It was therefore suggested that phosphates might be transported from the outer to inner compartments by the inoculated *** and then absorbed by the maize plant.

关键词： Colpoda cucullus root box ^(32)P phosphate fertilizer maize

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Contrasting resilience of soil microbial biomass,microbial diversity and ammonification enzymes under three applied soil fumigants

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Journal of Integrative Agriculture 2020年第10期19卷 2561-2570页

作者： SUN Zhen-cai li Gui-tong ZHANG Cheng-lei WANG Zhi-min liN Qi-mei zhao Xiao-rong college of Agronomy China Agricultural UniversityBejing 100193P.R.China college of Land science and technology China Agricultural UniversityBeijing 100193P.R.China Institute of Process Engineering Chinese Academy of SciencesBejing 100190P.R.China

Fumigation is a widely applied approach to mitigate the soil-borne ***,the potential effects of currently applied fumigants on ammonification remain *** 84-day incubation experiment was conducted based on non-fumigated soil(CK)and fumigated soil using three common fumigants,i.e.,chloropicrin(CP),1,3-dichloropropene(1,3-D),and metam sodium(MS).The results showed that,the three fumigants all decreased the microbial C,and the largest reduction(84.7%)occurred with the application of *** fumigation,the microbial diversity in the CP treatment rapidly recovered,but that in the 1,3-D treatment decreased and did not recover by the end of the *** application of MS showed no impact on the microbial diversity during the assay,indicating that significantly different microbial diversity can be achieved by choosing different ***,the three fumigants showed divergent effects on the enzymes involved in *** analysis showed that the enzyme variation with CP application was mainly associated with the changed microbial C and N(Plity index showed that CP was still significantly harmful at the end of incubation compared with the good resilience of MS,indicating that CP may not be a suitable fumigant.

关键词： chloropicrin 1,3-dichloropropene metam sodium N cycling microbial biomass and diversity

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SARM1 participates in axonal degeneration and mitochondrial dysfunction in prion disease

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Neural Regeneration Research 2022年第10期17卷 2293-2299页

作者： Meng-Yu Lai Jie li Xi-Xi Zhang Wei Wu Zhi-Ping li Zhi-Xin Sun Meng-Yang zhao Dong-Ming Yang Dong-Dong Wang Wen li De-Ming zhao Xiang-mei Zhou li-Feng Yang National animal Transmissible Spongiform Encephalopathy Laboratory College of Veterinary MedicineChina Agricultural UniversityBeijingChina

Prion disease represents a group of fatal neurogenerative diseases in humans and animals that are associated with energy loss,axonal degeneration,and mitochondrial *** degeneration is an early hallmark of neurodegeneration and is triggered by *** found that depletion or dysfunctional mutation of SARM1 protected against NAD+loss,axonal degeneration,and mitochondrial functional disorder induced by the neurotoxic peptide ***+supplementation rescued prion-triggered axonal degeneration and mitochondrial dysfunction and SARM1 overexpression suppressed this protective ***+supplementation in PrP106-126-incubated N2a cells,SARM1 depletion,and SARM1 dysfunctional mutation each blocked neuronal apoptosis and increased cell *** results indicate that the axonal degeneration and mitochondrial dysfunction triggered by PrP^(106-126) are partially dependent on SARM1 NADase *** pathway has potential as a therapeutic target in the early stages of prion disease.

关键词： axonal degeneration mitochondrial dysfunction NAD+metabolism NADase neurodegenerative disease prion disease SARM1 sterile alpha and TIR motif-containing 1

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食源性甲型肝炎病毒快速检测技术研究进展

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食品安全质量检测学报 2024年第6期15卷 74-82页

作者：魏莹杨艳歌赵健淞李涛李红娜孙冬梅袁飞黑龙江八一农垦大学生命科学技术学院大庆163000 中国检验检疫科学研究院国家市场监管重点实验室(食品质量与安全)北京100176

病毒在食源性疾病的爆发中起着至关重要的作用,其中,甲型肝炎病毒(hepatitis A virus,HAV)感染后能够致人衰弱,并引发急性肝衰竭。由于其传染迅速,影响范围广,HAV污染已经给消费者带来了严重的健康风险,且在全球范围内造成了大规模的食... 详细信息

病毒在食源性疾病的爆发中起着至关重要的作用,其中,甲型肝炎病毒(hepatitis A virus,HAV)感染后能够致人衰弱,并引发急性肝衰竭。由于其传染迅速,影响范围广,HAV污染已经给消费者带来了严重的健康风险,且在全球范围内造成了大规模的食源性疫情和经济损失。因此,早期快速准确的检测HAV对于食品安全和疫情暴发的溯源至关重要,以便及时确定并召回受污染的食品,防止感染的进一步发生。传统的HAV检测方法由于检出率不高以及病毒在细胞内的培养周期长等问题,往往耗时费力,无法快速有效地对其进行检测。本文对目前报道较多的HAV快速检测技术进行了阐述和梳理,对各项技术的检出限、检测时间以及优缺点进行了比较,并对HAV快速检测技术研究进行了展望,为后续HAV的快速检测研究提供依据,也有助于进一步探讨与开发HAV快速检测的新技术,以有效防止HAV的传播与危害。

关键词：甲型肝炎病毒快速检测分子生物学免疫学

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A comparative review of the state and advancement of Site-Specific Crop Management in the UK and china

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Frontiers of agricultural science and Engineering 2019年第2期6卷 116-136页

作者： Zhenhong li James TAYLOR Lynn FREWER chunjiang zhao Guijun YANG Zhenhai li Zhigang liU Rachel GAULTON Daniel WICKS Hugh MORTIMER Xiao CHENG Chaoqing YU Zhanyi SUN School of Engineering Newcastle University Newcastle upon Tyne NE1 7RU UK School of Natural and Environmental sciences Newcastle University Newcastle upon l\ne NE1 7RU UK National Engineering Research Center for Information technology in Agriculture Beijing Nongke Mansion Beijing 100097 China Courtyard Agriculture Ltd. Wiltshire SN3 5HY UK Satellite Applications Catapult Harwell OX11 0QR UK RAL Space Science and Technology Facilities Council Didcot 0X11 OQX UK State Key Laboratory of Remote Sensing science College of Global Change and Earth System Science Beijing Normal University Beijing 100875 China Center for Earth System science Tsinghua University Beijing 100084 China National Geomatics Center of china Beijing 100830 China

Precision agriculture, and more specifically Site-Specific Crop Management(SSCM), has been implemented in some form across nearly all agricultural production systems over the past 25 years. Adoption has been greatest in developed agricultural countries. In this review article, the current situation of SSCM adoption and application is investigated from the perspective of a developed(UK) and developing(china) agricultural economy. The current state-of-the art is reviewed with an emphasis on developments in position system technology and satellite-based remote sensing. This is augmented with observations on the differences between the use of SSCM technologies and methodologies in the UK and china and discussion of the opportunities for(and limitations to)increasing SSCM adoption in developing agricultural economies. A particular emphasis is given to the role of socio-demographic factors and the application of responsible research and innovation(RRI) in translating agritechnologies into china and other developing agricultural economies. Several key research and development areas are identified that need to be addressed to facilitate the delivery of SSCM as a holistic service into areas with low precision agriculture(PA) adoption. This has implications for developed as well as developing agricultural economies.

关键词： remote sensing decision support responsible research and innovation digital soil mapping

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Functional characterization of a novel, highly expressed ion-driven sugar antiporter in the thoracic muscles of Helicoverpa armigera

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Insect science 2022年第1期29卷 78-90页

作者： Yi-Yang Yuan Yu-Cui Xin Jian-li Han Yu-Han zhao Shi-Ming Han Zi-Yan Nangong Xin Chen Zhu-Cheng Wang mei li Xing-Hui Qiu State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijingChina college of life sciences University of Chinese Academy of SciencesBeijingChina college of life sciences Cangzhou Normal UniversityCangzhouJiangsu ProvinceChina college of Plant Protection Hebei Agricultural UniversityBaodingHebei ProvinceChina

Sugar transporters(STs),which mainly mediate cellular sugar exchanges,play critical physiological roles in living organisms,and they may be responsible for sugar exchanges among various insect ***,the molecular and physiological functions of insect STs are largely ***,16 STs of Helicoverpa armigera were identified.A phylogenetic analysis classified the putative HaSTs into 12 sub-families,and those identified in this study were distributed into 6 ***-time polymerase chain reaction indicated that the 16 HaSTs had diverse tissue-specific expression *** transporter,HaST10,was highly expressed in thoracic muscles.A functional study using a Xenopus oocyte expression system revealed that HaST10 mediated both H+-driven trehalose and Na+-driven glucose antiport activities with high transport efficiency and low affinity levels.A HaST10 knockout clearly impaired the performance of ***,HaST10 may participate in sugar-supply regulation and have essential physiological roles in ***.

关键词： CRISPR/Cas9 glucose Helicoverpa armigera sugar transporter trehalose thoracic muscles

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