Study on Tibetan Chicken embryonic adaptability to chronic hypoxia by revealing differential gene expression in heart tissue

作     者：LI Mei & ZHAO ChunJiang College of Animal Science and Technology,China Agricultural University,Beijing 100193,China 

作者机构：College of Animal Science and Technology China Agricultural University Beijing China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2009年第52卷第3期

页      面：284-295页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0905[农学-畜牧学] 09[农学] 

基　　金：Supported by National Key Basic Research and Development Program of China (Grant No.2006CB102101) the Project of National Fundamental Platform for Scientific Work (Grant No.2005DKA21100-02) 

主　　题：adaptation chronic hypoxia gene chip chicken genome array Tibetan Chicken(Gallus gallus) differential gene expression 

摘      要：Oxygen concentration is essential for appropriate *** can exert a significant impact on physiological alteration of the cell and *** Chicken(Gallus gallus) is a Chinese indigenous breed inhabiting in Tibetan areas,which is also a chicken breed living at high altitude for the longest time in the *** has developed an adaptive mechanism to hypoxia,which is demonstrated by that Tibetan Chicken has much higher hatchability than low-land chicken breeds in high-altitude areas of *** the present study,Tibetan Chicken fertilized full sib eggs were incubated up to Hamburger-Hamilton stage 43 under 13% and 21% oxygen concentration,*** Chicken and Dwarf Recessive White Chicken were used as control *** hearts in all of the 3 chicken breeds under hypoxic and normoxic conditions were isolated and hybridized to Genechip Chicken Genome Array to study molecular mechanisms underlying the adaptation to high altitude of Tibetan *** a result,50 transcripts highly expressed in hypoxia are screened *** up-regulated genes,some are involved in the gene ontology(GO) such as cell growth,cell difference,muscle contraction and signal ***,the expression levels of 21 transcripts are lower in hypoxia than those in *** down-regulated genes take part in cell communication,ion transport,protein amino acid phosphorylation and signal ***,gene enrichment analyses of these differential gene expressions are mainly associated with immune system response and ion channel activity in response to ***,the transcriptional expression profiles analyzed by hierarchical clustering and CPP-SOM software in all of the 3 different chicken breeds revealed that Tibetan Chicken is much closely related to Shouguang Chicken rather than Dwarf Recessive White *** addition,12 transcripts of Tibetan Chicken breed-specific expressed genes were identified,which seem to result in a more