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Chinese Journal of Structural Chemistry 2016年第2期35卷 286-292页

作者： KANG Hai-Xia FU Yu-Qin XIN ling-Yun li gui-lian MA Lu-Fang liU Guang-Zhen College of Chemistry and Chemical Engineering Luoyang Normal University

Two new coordination polymers,named {[Co3（pfca）6（phen）2]·2H2O}n（1） and[Cu（pfca）2（4,4＇-bpy）（H2O）]n（2）（Hpfca = 4-fluorocinnamic acid and 4,4-bpy = 4,4-bipyridine）,were hydrothermally synthesized and characterized by elemental analysis,IR spectroscopy,thermogravimetric analysis（TGA）,and single-crystal X-ray *** 1 crystallizes in the triclinic crystal system,space group P1 with a = 10.939（3）,b = 12.430（4）,c = 13.815（4） A,α=78.436（3）,β= 70.261（3）,γ= 80.268（3）°,V= 1721.8（9） A^3,Dc = 1.508 g/cm^3,Mr= 1564.06,Z = 1,F（000） = 799,μ= 0.802 mm^（-1）,the final R = 0.0432 and wR = 0.1015 for 13250 observed reflections with I 2σ（I）.Complex 2 is of monoclinic system,space group P2／lc with a =15.4985（14）,b = 15.8424（14）,c = 10.9528（10） A,β= 107.1600（10）°,V= 2569.6（4） A^3,Dc = 1.468g/cm^3,Mr = 568.02,Z = 4,F（000） = 1164,μ= 0.906 mm^（-1）,the final R = 0.0371 and wR = 0.0842 for 18183 observed reflections with I〉 2σ（I）.Complex 1 features linear Co（Ⅱ）-trinuclear units,which are assembled into a final 3D supramolecular structure through π…π stacking interactions and F-H weak *** 2 consists of 1D polymeric Cu（Ⅱ）-chains that are further cohered together by extensive H-bonds.

关键词： 4-fluorocinnamic acid coordination polymers trinuclear chainmagnetic property

Preliminary Study on Drug Susceptibility Profile and Resistance Mechanisms to Macrolides of Clinical Isolates of Non-tuberculous Mycobacteria from China

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Biomedical and Environmental Sciences 2018年第4期31卷 290-299页

作者： li Fu li gui lian PANG Hui liU Hai Can XIAO Tong Yang li Shuang Jun LUO Qiao JIANG Yi WANG Rui Bai WAN Kang lin State Key Laboratory for Infectious Diseases Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Immunology Department Changzhi Medical College Key Laboratory of Laboratory Medicine Wenzhou Medical University

Objective Macrolide susceptibility and drug resistance mechanisms of clinical non-tuberculous mycobacteria（NTM） isolates were preliminarily investigated for more accurate diagnosis and treatment of the infection in China. Methods Four macrolides, including clarithromycin（CLAR）, azithromycin（AZM）, roxithromycin（ROX）, and erythromycin（ERY）, were used to test the drug susceptibility of 310 clinical NTM isolates from six provinces of China with the broth microdilution method. Two resistance mechanisms, 23 S r RNA and erm, were analyzed with nucleotide sequence analysis. Results Varied effectiveness of macrolides and species-specific resistance patterns were observed. Most Mycobacterium abscessus subsp. massiliense were susceptible and all M. fortuitum were highly resistant to macrolides. All the drugs, except for erythromycin, exhibited excellent activities against slow-growing mycobacteria, and drug resistance rates were below 22.2%. Only four highly resistant strains harbored 2,058/2,059 substitutions on rrl and none of other mutations were related to macrolide resistance. G2191 A and T2221 C on rrl were specific for the M. abscessus complex（MABC）. Seven sites, G2140 A, G2210 C, C2217 G, T2238 C, T2322 C, T2404 C, and A2406 G, were specifically carried by M. avium and M. intracellulare. Three sites, A2192 G, T2358 G, and A2636 G, were observed only in M. fortuitum and one site G2152 A was specific for M. gordonae. The genes erm（39） and erm（41） were detected in M. fortuitum and M. abscessus and inducible resistance was observed in relevant sequevar. Conclusion The susceptibility profile of macrolides against NTM was demonstrated. The well-known macrolide resistance mechanisms, 23 S r RNA and erm, failed to account for all resistant NTM isolates, and further studies are warranted to investigate macrolide resistance mechanisms in various NTM species.

关键词： Non-tuberculous mycobacteria Macrolide Drug resistance

Immunological Evaluation of a Novel Mycobacterium tuberculosis Antigen Rv0674

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Biomedical and Environmental Sciences 2019年第6期32卷 427-437页

作者： XIAO Tong Yang liU Hai Can li Xiao Qin HUANG Ming Xiang li gui lian li Na YAN Yu Han LUO Qiao WANG Xue Zhi li Ma Chao WAN Kang lin State Key Laboratory for Infectious Diseases Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China Key Laboratory of Laboratory Medicine Ministry of Education College of Laboratory Medicine and Life Sciences Wenzhou Medical University Wenzhou 325035 Zhejiang China Clinical laboratory Fuzhou Pulmonary Hospital Fuzhou 350008 Fujian China The first hospital of Changsha Changsha 410005 Hunan China Key laboratory of Translational Medicine of Guangdong Center for Tuberculosis Control of Guangdong Province Guangzhou 510630 Guangdong China

Objective This study aimed to characterize the diagnostic and vaccine potential of a novel Mycobacterium tuberculosisantigen Rv0674. Methods To evaluate thediagnostic potential and antigenicity of Rv0674, IgG was evaluated using EliSA and interferon (IFN)-γ was done by using EliSpot assay among TB patients and healthy donors. For immunogenicity evaluation, BALB/c mice were immunized with Rv0674. Cytokine production was determined by cytokine release assay using an EliSA kit, and the antibodies were tested using EliSA. Results The results of serum Elisa tests showed that Rv0674 specific immunoglobulin G (IgG) response was higher in TB patients than negative controls. And Rv0674 had good performance in serological test with sensitivity and specificity of 77.1% and 81.1%, respectively. While it shows poor sensitivity and specificity of 26.23% and 79.69% for IFN-γ tests. In BALB/c mice, Rv0674 adjuvant by DDA/PolyI:C could also induce a high level of IFN-γ, interleukin-2 and interleukin-6 as well as a high IgG titer in both high-and low-dose groups indicating that Rv0674 is essential in humoral and cellular immunity. Moreover, the cytokine profile and IgG isotypecharacterized Rv0674 as a Th1/Th2-mixed-type protective immunity with the predominance of Th1 cytokines. Conclusion Rv0674 may be a good potential candidate for the development of TB serological diagnosis and a new TB vaccine.

关键词： Antigen Immunologic diagnosis Mycobacterium tuberculosis Rv0674 Vaccine

Immunogenicity of Whole Mycobacterium intracellulare Proteins and Fingding on the Cross-Reactive Proteins between *** and ***

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Biomedical and Environmental Sciences 2021年第7期34卷 528-539页

作者： XIAO Shi Qi XU Da DUAN Hong Yang FAN Xue Ting li gui lian ZHANG Wen li Ma Chao HAN Na li Xin Yao li Na ZHAO li lan ZHAO Xiu Qin WAN Kang lin liU Hai Can FENG Wen Hai State Key Laboratory of Agrobiotechnology Department of Microbiology and ImmunologyCollege of Biological SciencesChina Agricultural UniversityBeijing 100193China State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Department of Stomatology The Second Xiangya Hospital of Central South UniversityChangsha 410000HunanChina

Objectives To evaluate the immunogenicity of Mycobacterium intracellulare proteins and determine the cross-reactive proteins between *** and *** Protein extracts from *** were used to immunize BALB/c *** antigens were evaluated using cellular and humoral *** common genes between *** and *** were identified using genome-wide comparative analysis,and cross-reactive proteins were screened using immunoproteome *** Immunization with *** proteins induced significantly higher levels of the cytokines interferon-γ(IFN-γ),interleukin-2(IL-2),interleukin-12(IL-12),interleukin-6(IL-6)and immunoglobulins IgG,IgG1,IgM,and IgG2a in mouse *** marrow-derived macrophages isolated from mice immunized with *** antigens displayed significantly lower bacillary loads than those isolated from mice immunized with ***-genome sequence analysis revealed 396 common genes between *** and *** hybridization with *** proteins revealed the presence of 478 proteins in the serum of mice immunized with *** protein *** common antigens were found using both microchip and genomic comparative *** This is the advanced study to investigate the immunogenicity of *** proteins and the cross-reactive proteins between *** and *** results revealed the presence of a number of cross-reactive proteins between *** and ***,this study provides a new way of identifying immunogenic proteins for use in tuberculosis vaccines against both *** and *** in future.

关键词： Mycobacterium intracellulare Mycobacterium tuberculosis Cross-reactive immune response Vaccine Antigen

Rapid Detection of Rifampin-resistant Clinical Isolates of Mycobacterium tuberculosis by Reverse Dot Blot Hybridization

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Biomedical and Environmental Sciences 2015年第1期28卷 25-35页

作者： GUO Qian YU Yan ZHU Yan ling ZHAO Xiu Qin liU Zhi Guang ZHANG Yuan Yuan li gui lian WEI Jian Hao WU Yi Mou WAN Kang lin Pathogenic Biology Institute University of South China State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Dalian Maternity and Child Health Care Hospital (the Obstetrics and Gynecology Hospital of Dalian) Key Lab of Laboratory Medicine Wenzhou Medical College

Objective A PCR-reverse dot blot hybridization （RDBH） assay was developed for rapid detection of rpoB gene mutations in ＇hot mutation region＇ of Mycobacterium tuberculosis （M. tuberculosis）. Methods 12 oligonucleotide probes based on the wild-type and mutant genotype rpoB sequences of M. tuberculosis were designed to screen the most frequent wild-type and mutant genotypes for diagnosing RIF resistance. 300 M. tuberculosis clinical isolates were detected by RDBH, conventional drug-susceptibility testing （DST） and DNA sequencing to evaluate the RDBH assay. Results The sensitivity and specificity of the RDBH assay were 91.2% （165/181） and 98.3% （117/119）, respectively, as compared to DST. When compared with DNA sequencing, the accuracy, positive predictive value （PPV） and negative predictive value （NPV） of the RDBH assay were 97.7% （293/300）, 98.2% （164/167）, and 97.0% （129/133）, respectively. Furthermore, the results indicated that the most common mutations were in codons 531 （48.6%）, 526 （25.4%）, 516 （8.8%）, and 511 （6.6%）, and the combinative mutation rate was 15 （8.3%）. One and two strains of insertion and deletion were found among all strains, respectively. Conclusion Our findings demonstrate that the RDBH assay is a rapid, simple and sensitive method for diagnosing RIF-resistant tuberculosis.

关键词： Mycobacterium tuberculosis Rifampin-resistance Reverse dot blot hybridization

First Report in China on the Identification and Drug Sensitivity of Mycobacterium elephantis Isolated from the Milk of a Cow with Mastitis

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Biomedical and Environmental Sciences 2017年第7期30卷 501-507页

作者： JI ling Yun XU Dong Lei YIN Shu Peng liU Hai Can li gui lian JIANG Yi WEI Jian Hao ZENG Hao LOU Yong liang LYU Jian Xin WAN Kang lin Department of Laboratory Medicine Shanghai East Hospital Tongji University School of Medicine Shanghai 200123 China State Key Laboratory of Infectious Diseases Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China Key Laboratory of Laboratory Medicine Ministry of Education College of Laboratory Medicine and Life Sciences Wenzhou Medical University Wenzhou 325035 Zhejiang China Department of Clinical Laboratory Shanghai Public Health Clinical Center Affiliated to Fudan University Shanghai 201508 China Department of Spine Surgery The Xiangya Hospital of Central South University Changsha 410008 Hunan China

Objective In this study, milk from a cow with mastitis was analyzed to determine the presence of mycobacterial infection. Milk quality and security problems pertaining to the safe consumption of dairy products were also discussed in this study. Methods Milk was preprocessed with 4% NaOH. Then, mycobacteria were isolated from the milk sample on L-J medium. The isolate was identified using multiple loci Polymerase Chain Reaction （PCR） and multi-locus sequence analysis with 16S rRNA, sodA, hsp65, and ITS genes. The drug sensitivity of the isolate to 27 antibiotics was tested through alamar blue assay. Results Smooth, moist, pale yellow colonies appeared on the L-J medium within a week after inoculation. Based on the results of multiple loci PCR analysis, the isolate was preliminarily identified as non-tuberculous mycobacteria. The 16S rRNA, SodA, hsp65, and ITS gene sequences of the isolate exhibited 99%, 99%, 99%, and 100% similarities, respectively, with those of the published reference strains of Mycobacteriurn elephantis （M. elephantis）. The drug sensitivity results showed that the strain is resistant to isoniazid, p-aminosalicylic acid, and trimesulf but is sensitive to ofloxacin, rifampicin, amikacin, capreomycin, moxifloxacin, kanamycin, levofloxacin, cycloserine, ethambutol, streptomycin, tobramycin, rifabutin, ciprofloxacin, linezolid, cefoxitin, clarithromycin, and minocycline. Conclusion To the best of our knowledge, this study is initially to report the isolation of M. elephantis from the milk of a cow with mastitis in China.

关键词： Key words: Non-tuberculosis mycobacterium Mycobacterium elephantis Milk Cow mastitis

“谁解绘其真”:端恩的题红诗与清宗室文人社会心理

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南京师大学报（社会科学版） 2018年第6期 134-141页

作者：李贵连浙江越秀外国语学院

文学是生活的间接反映,一个群体的文化特质往往和其所面对的问题及处境息息相关。清宗室文人既是封建君主集权体系中专制政体的延伸,又在天潢贵胄闲适优裕生活的另一面承载着皇权的势压和政治倾轧所带来的焦虑紧张。娴熟笔墨的学植修养... 详细信息

文学是生活的间接反映,一个群体的文化特质往往和其所面对的问题及处境息息相关。清宗室文人既是封建君主集权体系中专制政体的延伸,又在天潢贵胄闲适优裕生活的另一面承载着皇权的势压和政治倾轧所带来的焦虑紧张。娴熟笔墨的学植修养使他们习惯了用文字呈现志趣、交心友朋的生活方式,自然也常用诗歌的咏叹抒散内心的惶惑和隐忧。曹雪芹作为汉军旗人的特定身份及其在宗室文人交际圈的辗转声名传播,给端恩等宗室文人以族群身份认同感和斯人宛在的亲切感;《红楼梦》在预示封建体制没落和崩塌的同时,也给他们无可奈何的命运代入感。宗室文人关于《红楼梦》的题咏在表现他们闲适雅致情趣的同时,更体现了这一群体所濡染的儒家事功观念和多发性的投闲置散两者之间所激发出的人生如梦的惶惑。

关键词：端恩宗室文人题红诗社会心理

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清代满人经学成就述论

河南师范大学学报（哲学社会科学版） 2012年第4期39卷 152-155页

作者：李贵连南京大学文学院江苏南京210093

儒家经典是中国古代治国安邦的神圣准则和重要依据,作为清朝统治阶级的满人,亦非常重视学习儒学经典及广用儒臣。上至皇室诸王,下至八旗生员,经学专才辈出,撰有多部经学论著。涉猎范围遍及《五经》《四书》等,而尤以对易学的广研精索及... 详细信息

儒家经典是中国古代治国安邦的神圣准则和重要依据,作为清朝统治阶级的满人,亦非常重视学习儒学经典及广用儒臣。上至皇室诸王,下至八旗生员,经学专才辈出,撰有多部经学论著。涉猎范围遍及《五经》《四书》等,而尤以对易学的广研精索及理学的体察践求为著。满人对经学的崇尚与翻译引致小学的昌大繁盛,其中对满文的辨析考释又让他们尤为热衷。学风上趋兼采,重实证,则颇现朴学思潮之波及。

关键词：清代满人经学成就