Immunological Evaluation of a Novel Mycobacterium tuberculosis Antigen Rv0674

作     者：XIAO Tong Yang LIU Hai Can LI Xiao Qin HUANG Ming Xiang LI Gui Lian LI Na YAN Yu Han LUO Qiao WANG Xue Zhi LI Ma Chao WAN Kang Lin 

作者机构：State Key Laboratory for Infectious Diseases Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China Key Laboratory of Laboratory Medicine Ministry of Education College of Laboratory Medicine and Life Sciences Wenzhou Medical University Wenzhou 325035 Zhejiang China Clinical laboratory Fuzhou Pulmonary Hospital Fuzhou 350008 Fujian China The first hospital of Changsha Changsha 410005 Hunan China Key laboratory of Translational Medicine of Guangdong Center for Tuberculosis Control of Guangdong Province Guangzhou 510630 Guangdong China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2019年第32卷第6期

页      面：427-437页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：National Science and Technology Major Project of China [2018ZX10731301-002] 

主　　题：Antigen Immunologic diagnosis Mycobacterium tuberculosis Rv0674 Vaccine 

摘      要：Objective This study aimed to characterize the diagnostic and vaccine potential of a novel Mycobacterium tuberculosisantigen Rv0674. Methods To evaluate thediagnostic potential and antigenicity of Rv0674, IgG was evaluated using ELISA and interferon (IFN)-γ was done by using ELISpot assay among TB patients and healthy donors. For immunogenicity evaluation, BALB/c mice were immunized with Rv0674. Cytokine production was determined by cytokine release assay using an ELISA kit, and the antibodies were tested using ELISA. Results The results of serum Elisa tests showed that Rv0674 specific immunoglobulin G (IgG) response was higher in TB patients than negative controls. And Rv0674 had good performance in serological test with sensitivity and specificity of 77.1% and 81.1%, respectively. While it shows poor sensitivity and specificity of 26.23% and 79.69% for IFN-γ tests. In BALB/c mice, Rv0674 adjuvant by DDA/PolyI:C could also induce a high level of IFN-γ, interleukin-2 and interleukin-6 as well as a high IgG titer in both high-and low-dose groups indicating that Rv0674 is essential in humoral and cellular immunity. Moreover, the cytokine profile and IgG isotypecharacterized Rv0674 as a Th1/Th2-mixed-type protective immunity with the predominance of Th1 cytokines. Conclusion Rv0674 may be a good potential candidate for the development of TB serological diagnosis and a new TB vaccine.