Immunogenicity of Whole Mycobacterium intracellulare Proteins and Fingding on the Cross-Reactive Proteins between *** and ***

作     者：XIAO Shi Qi XU Da DUAN Hong Yang FAN Xue Ting LI Gui Lian ZHANG Wen LI Ma Chao HAN Na LI Xin Yao LI Na ZHAO Li lan ZHAO Xiu Qin WAN Kang Lin LIU Hai Can FENG Wen Hai XIAO Shi Qi;XU Da;DUAN Hong Yang;FAN Xue Ting;LI Gui Lian;ZHANG Wen;LI Ma Chao;HAN Na;LI Xin Yao;LI Na;ZHAO Li lan;ZHAO Xiu Qin;WAN Kang Lin;LIU Hai Can;FENG Wen Hai

作者机构：State Key Laboratory of AgrobiotechnologyDepartment of Microbiology and ImmunologyCollege of Biological SciencesChina Agricultural UniversityBeijing 100193China State Key Laboratory for Infectious Disease Prevention and ControlNational Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Department of StomatologyThe Second Xiangya Hospital of Central South UniversityChangsha 410000HunanChina 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2021年第34卷第7期

页      面：528-539页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by National Science and Technology Major Project of China[2018ZX10731301-002] 

主　　题：Mycobacterium intracellulare Mycobacterium tuberculosis Cross-reactive immune response Vaccine Antigen 

摘      要：Objectives To evaluate the immunogenicity of Mycobacterium intracellulare proteins and determine the cross-reactive proteins between *** and *** Protein extracts from *** were used to immunize BALB/c *** antigens were evaluated using cellular and humoral *** common genes between *** and *** were identified using genome-wide comparative analysis,and cross-reactive proteins were screened using immunoproteome *** Immunization with *** proteins induced significantly higher levels of the cytokines interferon-γ(IFN-γ),interleukin-2(IL-2),interleukin-12(IL-12),interleukin-6(IL-6)and immunoglobulins IgG,IgG1,IgM,and IgG2a in mouse *** marrow-derived macrophages isolated from mice immunized with *** antigens displayed significantly lower bacillary loads than those isolated from mice immunized with ***-genome sequence analysis revealed 396 common genes between *** and *** hybridization with *** proteins revealed the presence of 478 proteins in the serum of mice immunized with *** protein *** common antigens were found using both microchip and genomic comparative *** This is the advanced study to investigate the immunogenicity of *** proteins and the cross-reactive proteins between *** and *** results revealed the presence of a number of cross-reactive proteins between *** and ***,this study provides a new way of identifying immunogenic proteins for use in tuberculosis vaccines against both *** and *** in future.