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Influence of hypoxia-inducible factor 1-alpha on neuronal apoptosis in a rat model of hypoxia-or hypoxia-ischemia-induced brain injury

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Neural Regeneration Research 2009年第12期4卷 1019-1023页

作者： lihua li Yi Qu li Zhang xihong li Jinhui li Meng Mao Xiudong Jin Dezhi Mu Department of Pediatrics West China Second Hospital Sichuan University Chengdu 610041 Sichuan Province China Departrnent of Pediatrics Bayi Children's Hospital Military General Hospital of Beijing PLA Beijing 100700 China Mudanjiang Medical College Mudanjiang 157011 Heilongjiang Province China

BACKGROUND： In addition to neuroprotective genes, the targeted genes of hypoxia-inducible factor 1α （HIF-1α） include pro-apoptotic genes. However, the influence of HIF-1α on neuronal apoptosis in hypoxia-ischemia remains poorly understood. OBJECTIVE： To investigate the relationship between HIF-1α expression and neuronal apoptosis in hypoxia or hypoxia-ischemia brain injury and to determine the role of HIF-1α in regulating neuronal apoptosis. DESIGN, TIME AND SETTING： A randomized, controlled animal experiment was performed at the Laboratory of Children Neurology of Sichuan University between May 2006 and May 2007. MATERIALS： In situ cell death detected kit was provided by Roche, USA; rabbit anti-mouse HIF-1α polyclonal antibody was purchased from Santa Cruz Biotechnologies, USA; rabbit anti-mouse cleaved caspase-3 polyclonal antibody was purchased from Chemicon, USA. METHODS： A total of 36 Sprague Dawley rats aged 10 days were randomly assigned to 3 groups： sham-surgery, hypoxia, and hypoxia-ischemia, with 12 rats per group. The rats were treated at 3 time points： 4, 8, and 24 hours, with 4 rats per time point. In the hypoxia-ischemia group, the right common carotid artery was exposed and permanently ligated through a midline cervical incision. A 2.5-hour exposure to hypoxia （8% O2/92% N2） was used to induce hypoxia-ischemia injury. In the hypoxia group, rats were exposed to hypoxia without ligation of the common carotid artery. In the sham-surgery group, the common carotid artery was exposed without ligation or hypoxia. MAIN OUTCOME MEASURES： Histopathological changes, HIF-1α and activated caspase-3 protein expression, integrated optical density of positive cells, and apoptosis-positive cells. RESULTS： Hematoxylin and eosin staining showed that neuronal degeneration and edema was most prominent at 24 hours after hypoxia-ischemia. HIF-1α protein expression was significantly upregulated at 4 hours, peaked at 8 hours, and decreased at 24 hours after hypoxia or hypoxia

关键词： HIF-1α apoptosis hypoxia ischemia brain damage

A kinetic model for predicting shelf-life of fresh extruded rice-shaped kernels(FER)

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Grain & Oil Science and Technology 2022年第4期5卷 187-193页

作者： Lu li Xuejin li Ge Gao Yiming Yan Xiaodong Wang Yao Tang Yuqian Jiang xihong li State Key Laboratory of Food Nutrition and Safety Tianjin University of Science and TechnologyTianjin 300457China College of Bioengineering Tianjin University of Science and TechnologyTianjin 300457China

Fresh extruded rice-shaped kernels(FER) are remoulded rice products from cereals or seed flours, which have the advantages of safety, nutrition, health and time saving. However, the finished products are easy to react with oxygen, so it is necessary to develop a fast, simple and reliable approach to monitor and predict the shelf-life of FER. A comprehensive mathematical model of FER shelf-life prediction was developed using a dynamic modelling approach based on real supply chain conditions. This predictive model was developed to determine four key indexes including acid value, iodine blue value, water uptake ratio and peroxide value. The results showed that when the peroxide value was 1.6849, the FER lost its edible value, nutritional value and commodity value. Moreover, the acid value and peroxide value of FER were used to establish a first-order kinetic model, and the iodine blue value of FER was suited for a zero-order kinetic model. The validation experiment of predicted and measured shelf life showed that the relative error was 3.12%, which was less than 5%. Therefore, this kinetic model could be used to predict the shelf-life of FER quickly and conveniently. The kinetic-based shelf-life prediction model proposed in this study is rapid and practical, providing theoretical basis and guidance for the establishment of quality monitoring and quality evaluation systems of FER during the production, storage, transport and marketing.

关键词： Fresh extruded rice-shaped kernels Shelf life Kinetic model Rice ageing Predicting

iTRAQ-based analysis of 17β-estradiol induced proteome in Chinese tongue sole Cynoglossus semilaevis

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Journal of Oceanology and limnology 2019年第5期37卷 1659-1668页

作者： ZHU Ying li Yangzhen li Hailong WANG Lei ZHANG Ning liU Yang MENG liang XU Xiwen DONG Zhongdian WEI Min GUO Hua CUI Zhongkai li xihong SHAO Changwei XU Wenteng Key Laboratory of Sustainable Development of Marine Fisheries Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Research Institute of Metabolic Disease Qingdao UniversityQingdao 266003China

The phenomenon of sex dimorphism prevails among many fi sh species. It has attracted the general research interest due to its close relationship to fi sh growth and thus aquaculture productivity. In Chinese tongue sole ( Cynoglossus semilaevis ), 17β-estradiol (E2) can be used reportedly to induce the feminization of fi sh, although the detailed regulatory network remained elusive. We treated the tongue sole fry before sex diff erentiation with E2 (10 and 30 μg/L) to identify potential targets of E2 in Chinese tongue sole. The E2 treatment indeed induced genetic male fi sh sex-reversal to phenotypic female. Using an iTRAQ-based comparative proteomic analysis, 409 proteins that diff erentially expressed after E2 induction were identifi ed, including 259 up-regulated and 150 down-regulated proteins. Furthermore, 19 diff erentially expressed proteins identifi ed in the comparative proteomic analysis were selected to assess their transcription and eight of them exhibited the same tendency. Functions of the eight proteins included mainly nucleotide and protein binding. Interestingly, a far upstream element-binding protein 3-like isoform exhibited the signifi cant upregulation both at transcription and translation levels after E2 treatment. This work identifi ed a set of candidate proteins for E2 response and deepened our understanding of E2 regulatory mechanism.

关键词： Cynoglossus semilaevis 17β-estradiol sex reversal proteome iTRAQ analysis

Promotional effects of Er incorporation in CeO_2(ZrO_2)/TiO_2 for selective catalytic reduction of NO by NH_3

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Chinese Journal of Catalysis 2016年第9期37卷 1521-1529页

作者： Qijie Jin Yuesong Shen Shemin Zhu xihong li Min Hu Jiangsu Collaborative Innovation Center for Advanced Inorganic Function Composites Jiangsu National Synergetic Innovation Center for Advanced Materials （SICAM） College of Materials Science and Engineering Nanjing Tech University Nanjing 210009 Jiangsu China Shandong Gemsky Environmental Technology Co. Zibo 255086 Shandong China

A series CeO2（ZrO2）/TiO2 catalysts were modified with Er using a sol-gel *** catalytic activity of the obtained catalysts in the selective catalytic reduction（SCR） of NO with NH3 was investigated to determine the appropriate Er *** catalysts were characterized using X-ray diffraction,N2 adsorption,NH3 temperature-programmed desorption,H2 temperature-programmed reduction,photoluminescence spectroscopy,electron paramagnetic resonance spectroscopy,and X-ray photoelectron *** results showed that the optimum Er/Ce molar ratio was 0.10;this catalyst had excellent resistance to catalyst poisoning caused by vapor and sulfur and gave more than 90% NO conversion at 220–395 ℃ and a gas hourly space velocity of 71 400 h^-*** incorporation increased the Ti^3＋ concentrations,oxygen storage capacities,and oxygen vacancy concentrations of the catalysts,resulting in excellent catalytic *** incorporation also decreased the acid strength and inhibited growth of TiO2 and CeO2 crystal particles,which increased the catalytic *** results show that high oxygen vacancy concentrations and oxygen storage capacities,large amounts of Ti^3＋,and low acid strengths give excellent SCR activity.

关键词： CeO2(ZrO2)/TiO2 Erbium incorporation Selective catalytic reduction Nitrogen oxide Catalytic performance

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不锈钢弹簧座的离心铸坯-热挤压成形技术

特种铸造及有色合金 2009年第5期29卷 444-445页

作者：李喜红河北机电职业技术学院

通过对离心铸坯、热挤压成形技术特点的分析及不锈钢机械密封弹簧座离心铸坯-热挤压联合成形新工艺实践和经济效益的研究,表明离心铸坯-热挤压联合成形新工艺,发挥离心铸造、挤压两种工艺的优势,具有缩短工艺流程、提高材料利用率、节... 详细信息

通过对离心铸坯、热挤压成形技术特点的分析及不锈钢机械密封弹簧座离心铸坯-热挤压联合成形新工艺实践和经济效益的研究,表明离心铸坯-热挤压联合成形新工艺,发挥离心铸造、挤压两种工艺的优势,具有缩短工艺流程、提高材料利用率、节约能源、改善制件内在品质等优点。生产100t成品可节约材料费300余万元。

关键词：不锈钢离心铸造热挤压成形工艺优化

3 - 96 Anti-Porcine Respiratory and Reproductive Syndrome Virus Effects of Extract from Isatis Indigotica Fortune

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IMP & HIRFL Annual Report 2010年第1期 201-202页

作者： liang Jiangping Zhou Xiang li Xuehu Lu xihong

3 - 95 Strain Improvements Trichoderma viride for of Aspergillus niger and Increased Cellulase Production by Irradiation of Electron and 12C6＋-ion Beams

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IMP & HIRFL Annual Report 2010年第1期 200-201页

作者： liang Jianping li Zhaozhou li Xuehu Lu xihong Zhou Xiang

3 - 94 Non-specific Amplification Analysis （L25436） Chlamydia Irradiated by Heavy Carbon Ion

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IMP & HIRFL Annual Report 2010年第1期 198-199页

作者： Zhou Xiang liang Jianping li Xuehu Lu xihong Wang Shuyang

Study on Optimizing Nutrition and Fermentation Conditions for Compound <i>Bacillus spp.</i>

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American Journal of Molecular Biology 2019年第2期9卷 75-84页

作者： Yanyan li Yuxiang Xu Wenhao li Yong Yang lin Wang Jun Yu Changjun Wang xihong li Tobacco Research Institute of Hubei Province Wuhan China The Key Lab of Plant Pathology of Hubei Province College of Plant Science and Technology Huazhong Agricultural University Wuhan China Hubei University Wuhan China Hubei Tobacco Industry Co. Ltd. Wuhan China

In order to improve the spore yield of compound Bacillus spp. (B. amyloliquefaciens, B. laterosporus and B. megaterium), the effects of nutrient conditions including carbon source, nitrogen source, mineral salt and fermentation conditions including the inoculum age, inoculation amount, loading volume of liquid and initial pH on the spore yield were studied. The results indicated that the optimized medium was glucoses 20 g/L, soybean meal 30.0 g/L, K2HPO4 1.0 g/L;fermentation temperature is 37℃, the inoculum age 12 h, initial pH 7.0, 2% inoculation amount, loading volume of liquid 20 mL/250 mL. Under the optimized conditions of culture medium and fermentation for compound Bacillus spp., spore yield was 10.24 times more than the initial medium, and the spore formation rate reached more than 90%.

关键词： Compound Bacillus spp. Spore Yield Medium Optimization Fermentation Optimization