Study on Optimizing Nutrition and Fermentation Conditions for Compound <i>Bacillus spp.</i>

作     者：Yanyan Li Yuxiang Xu Wenhao Li Yong Yang Lin Wang Jun Yu Changjun Wang Xihong Li 

作者机构：Tobacco Research Institute of Hubei Province Wuhan China The Key Lab of Plant Pathology of Hubei Province College of Plant Science and Technology Huazhong Agricultural University Wuhan China Hubei University Wuhan China Hubei Tobacco Industry Co. Ltd. Wuhan China 

出 版 物：《American Journal of Molecular Biology》 (美国分子生物学期刊（英文）)

年 卷 期：2019年第9卷第2期

页      面：75-84页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Compound Bacillus spp. Spore Yield Medium Optimization Fermentation Optimization 

摘      要：In order to improve the spore yield of compound Bacillus spp. (B. amyloliquefaciens, B. laterosporus and B. megaterium), the effects of nutrient conditions including carbon source, nitrogen source, mineral salt and fermentation conditions including the inoculum age, inoculation amount, loading volume of liquid and initial pH on the spore yield were studied. The results indicated that the optimized medium was glucoses 20 g/L, soybean meal 30.0 g/L, K2HPO4 1.0 g/L;fermentation temperature is 37℃, the inoculum age 12 h, initial pH 7.0, 2% inoculation amount, loading volume of liquid 20 mL/250 mL. Under the optimized conditions of culture medium and fermentation for compound Bacillus spp., spore yield was 10.24 times more than the initial medium, and the spore formation rate reached more than 90%.