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iTRAQ-based analysis of 17β-estradiol induced proteome in Chinese tongue sole Cynoglossus semilaevis

iTRAQ-based analysis of 17β-estradiol induced proteome in Chinese tongue sole Cynoglossus semilaevis

作     者:ZHU Ying LI Yangzhen LI Hailong WANG Lei ZHANG Ning LIU Yang MENG Liang XU Xiwen DONG Zhongdian WEI Min GUO Hua CUI Zhongkai LI Xihong SHAO Changwei XU Wenteng 

作者机构:Key Laboratory of Sustainable Development of Marine FisheriesMinistry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production ProcessesQingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Research Institute of Metabolic DiseaseQingdao UniversityQingdao 266003China 

出 版 物:《Journal of Oceanology and Limnology》 (海洋湖沼学报(英文))

年 卷 期:2019年第37卷第5期

页      面:1659-1668页

核心收录:

学科分类:0908[农学-水产] 09[农学] 

基  金:Supported by the Natural Science Foundation of Shandong Province(No.ZR2014CQ053) the National Natural Science Foundation of China(No.31402293) the Taishan Scholar Climbing Program of Shandong Province,China 

主  题:Cynoglossus semilaevis 17β-estradiol sex reversal proteome iTRAQ analysis 

摘      要:The phenomenon of sex dimorphism prevails among many fi sh species. It has attracted the general research interest due to its close relationship to fi sh growth and thus aquaculture productivity. In Chinese tongue sole ( Cynoglossus semilaevis ), 17β-estradiol (E2) can be used reportedly to induce the feminization of fi sh, although the detailed regulatory network remained elusive. We treated the tongue sole fry before sex diff erentiation with E2 (10 and 30 μg/L) to identify potential targets of E2 in Chinese tongue sole. The E2 treatment indeed induced genetic male fi sh sex-reversal to phenotypic female. Using an iTRAQ-based comparative proteomic analysis, 409 proteins that diff erentially expressed after E2 induction were identifi ed, including 259 up-regulated and 150 down-regulated proteins. Furthermore, 19 diff erentially expressed proteins identifi ed in the comparative proteomic analysis were selected to assess their transcription and eight of them exhibited the same tendency. Functions of the eight proteins included mainly nucleotide and protein binding. Interestingly, a far upstream element-binding protein 3-like isoform exhibited the signifi cant upregulation both at transcription and translation levels after E2 treatment. This work identifi ed a set of candidate proteins for E2 response and deepened our understanding of E2 regulatory mechanism.

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