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Bacteriophage cocktail supplementation improves growth performance,gut microbiome and production traits in broiler chickens

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Journal of Animal Science and Biotechnology 2021年第4期12卷 1339-1350页

作者： Santi Devi Upadhaya Je Min ahn Jae Hyoung cho Jin Young Kim Dae kyung Kang Sung Woo Kim Hyeun Bum Kim In Ho Kim Department of Animal Resource and Science Dankook UniversityNo.29 AnseodongCheonanChoongnam 31116South Korea Department of Animal Science North Carolina State University116 Polk HallBox 7621120 W Broughton DrRaleighNC 27695USA

Background:Effective antibiotic alternatives are urgently needed in the poultry industry to control disease *** therapy mainly utilizes lytic phages to kill their respective bacterial hosts and can be an attractive solution to combating the emergence of antibiotic resistance in ***:Five hundred and four,one-day-old broilers(Ross 308)were allotted to 1 of 4 treatment groups in a completely randomized *** consisted of CON(basal diet),PC(CON+0.025%Avilamax®),BP 0.05(CON+0.05%bacteriophage),and BP 0.10(CON+0.10%bacteriophage).Results:A significant linear effect on body weight gain(BWG)was observed during days 1–7,days 22–35,and cumulatively in bacteriophage(BP)supplemented *** BWG tended to be higher(P=0.08)and the feed intake(FI)was increased(P=0.017)in the PC group over CON group.A greater(P=0.016)BWG and trends in increased FI(P=0.06)were observed in the experiment in birds fed PC than CON *** the genus level,the relative abundance of Lactobacillus was decreased in PC(65.28%),while it was similar in BP 0.05 and BP 0.10(90.65%,86.72%)compared to CON(90.19%).At the species level,the relative abundance of Lactobacillus salivarus was higher in BP 0.05(40.15%)and BP 0.10(38.58%)compared to the CON(20.04%)and PC(18.05%).A linear reduction in the weight of bursa of Fabricius(P=0.022)and spleen(P=0.052)was observed in birds fed graded level of BP and an increase(P=0.059)in the weight of gizzard was observed in birds fed PC over BP *** and quadratic responses were observed in redness of breast muscle color in birds fed graded level of ***:The inclusion of the 0.05%and 0.1%BP cocktail linearly improved broiler weight during the first 7 days,22–35 days and cumulatively,whereas 0.05%BP addition was sufficient for supporting immune organs,bursa and spleen as well as enhancing gut microbiome,indicating the efficacy of 0.05%BP as a substitute antibiotic growth promoter in broiler diets.

关键词： Bacteriophage Broiler chickens Gut microbiome Performance

Fiber-based quantum-dot pulse oximetry for wearable health monitoring with high wavelength selectivity and photoplethysmogram sensitivity

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npj Flexible Electronics 2023年第1期7卷 413-424页

作者： Ho Seung Lee Byeongju Noh Seong Uk Kong Yong Ha Hwang Ha-Eun cho Yongmin Jeon kyung Cheol choi School of Electrical Engineering Korea Advanced Institute of Science and Technology(KAIST)DaejeonRepublic of Korea Department of Biomedical Engineering Gachon UniversitySeongnamRepublic of Korea

Increasing demand for real-time healthcare monitoring is leading to advances in thin and flexible optoelectronic device-based wearable pulse *** previous studies have used OLEDs for this purpose,but did not consider the side effects of broad full-width half-maximum(FWHM)characteristics and single *** this study,we performed SpO_(2)measurement using a fiber-based quantum-dot pulse oximetry(FQPO)system capable of mass production with a transferable encapsulation technique,and a narrow FWHM of about 30 *** on analyses we determined that uniform angular narrow FWHM-based light sources are important for accurate SpO_(2)measurements through multi-layer structures and human skin *** FQPO was shown to have improved photoplethysmogram(PPG)signal sensitivity with no waveguide-mode noise signal,as is typically generated when using a single substrate(30–50%).We successfully demonstrate improved SpO_(2)measurement accuracy as well as all-in-one clothing-type pulse oximetry with FQPO.

关键词： pulse waveguide quantum

Anemia after gastrectomy for early gastric cancer:Long-term follow-up observational study

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World Journal of Gastroenterology 2012年第42期18卷 6114-6119页

作者： Chul-Hyun Lim Sang Woo Kim Won Chul Kim Jin Soo Kim Yu kyung cho Jae Myung Park In Seok Lee Myung-Gyu choi Kyo-Young Song Hae Myung Jeon cho-Hyun Park Department of Internal Medicine The Catholic University of Korea College of MedicineSeoul 137-701South Korea Department of Surgery The Catholic University of Korea College of MedicineSeoul 137-701South Korea

AIM:To identify the incidence and etiology of anemia after gastrectomy in patients with long-term follow-up after gastrectomy for early gastric ***:The medical records of those patients with early gastric adenocarcinoma who underwent curative gastrectomy between January 2006 and October 2007 were *** with anemia in the preoperative workup,cancer recurrence,undergoing systemic chemotherapy,with other medical conditions that can cause anemia,or treated during follow up with red cell transfusions or supplements for anemia were *** was defined by World Health Organization criteria(Hb 100 fL) with vitamin B 12 *** profile of anemia over 48 mo of follow-up was ***:One hundred sixty-one patients with gastrectomy for early gastric cancer were *** incidence of anemia was 24.5% at 3 mo after surgery and increased up to 37.1% at 48 mo after *** incidence of iron deficiency anemia increased during the follow up and became the major cause of anemia at 48 mo after *** of chronic disease and megaloblastic anemia were *** incidence of anemia in female patients was significantly higher than in male patients at 12(40.0% vs 22.0%,P = 0.033),24(45.0% vs 25.0%,P = 0.023),36(55.0% vs 28.0%,P = 0.004),and 48 mo(52.0% vs 31.0%,P = 0.022) after *** with total gastrectomy showed significantly higher incidence of anemia than patients with subtotal gastrectomy at 48 mo after surgery(60.7% vs 31.3%,P = 0.008).The incidence of iron deficiency was significantly higher in female patients than in male patients at 6(35.4% vs 13.3%,P = 0.002),12(45.8% vs 16.8%,P

关键词： Gastrectomy Stomach neoplasms Anemia Iron deficiency Vitamin B 12 deficiency

Successful endoscopic submucosal dissection of a giant polyp in a 21-month-old female

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World Journal of Gastroenterology 2014年第1期20卷 323-325页

作者： Eun-Young Jung Soon-Ok choi Kwang Bum cho Eun Soo Kim kyung Sik Park Jin-Bok Hwang Department of Pediatric Surgery Division of Gastroenterology and HepatologyKeimyung University School of MedicineDaegu 700-712South Korea Department of Internal Medicine Division of Gastroenterology and HepatologyKeimyung University School of MedicineDaegu 700-712South Korea Division of Gastroenterology and Hepatology Department of PediatricsKeimyung University School of MedicineDaegu 700-712South Korea

Endoscopic submucosal dissection(ESD)is now recognized as the preferred treatment modality for gastrointestinal epithelial lesions.A 21-month-old female was admitted with a giant hyperplastic polyp causing a gastric outlet *** ESD was performed with *** post-procedural course was uneventful without a bleeding *** further study of the feasibility of ESD in early children is necessary,ESD could be applied to avoid laparotomy even in young children.

关键词： Obstruction Endoscopic submucosal dissection Infant Hyperplastic polyp

Phase separations in graded-indium content InGaN/GaN multiple quantum wells and its function to high quantum efficiency

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Journal of Semiconductors 2012年第5期33卷 13-16页

作者：郭洪英孙元平 Yong-Hoon cho Eun-kyung Suh Hai-Joon Lee Rak-Jun choi Yoon-Bong Hahn Institute of Science and Technology for Opto-Electronic Information Yantai University Department of Physics Korea Advanced Institute of Science and Technology School of Semiconductor and Chemical Engineering Semiconductor Physics Research CenterChonbuk National University School of Chemical Engineering Chonbuk National University

Phase separations have been studied for graded-indium content In_xGa_（1-x）N/GaN multiple quantum wells（MQWs） with different indium contents by means of photoluminescence（PL）,cathodeluminescence（CL） and time-resolved PL（TRPL） *** the main emission peaks,all samples show another 2 peaks at the high and low energy parts of the main peaks in PL when excited at 10 *** images show a clear contrast for 3 samples,which indicates an increasing phase separation with increasing indium *** spectra at 15 K of the main emissions show an increasing delay of rising time with indium content,which means a carrier transferring from low indium content structures to high indium content structures.

关键词： carrier transfer phase separation graded-indium content multiple quantum wells

Acoustic radiation force impulse elastography for hepatocellular carcinoma-associated radiofrequency ablation

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World Journal of Gastroenterology 2011年第14期17卷 1874-1878页

作者： Hee-Jin Kwon Myong-Jin Kang Jin-Han cho Jong-Young Oh kyung-Jin Nam Sang-Yeong Han Sung Wook Lee Department of Radiology Dong-A University HospitalDongdaesindong 3gaSeo-guPusan 602-715South Korea Department of Internal Medicine Dong-A University HospitalDongdaesindong 3gaSeo-guPusan 602-715South Korea

AIM:To evaluate the potential usefulness of acoustic radiation force impulse (ARFI) images for evaluation of hepatocellular carcinomas (HCC)-associated radiofrequency ablation. METHODS:From January 2010 to June 2010,a total of 38 patients with HCC including recurred HCCs after RFA underwent ARFI elastography. The brightness of tumor was checked and the shear wave velocity was measure-d for the-quantification of stiffne-ss. According to theb-rightne-ss,the-tumors we-re-classifie-d as b-righte-r,samecolor and darker compared with adjacent parenchyma. Using the same methods,8 patients with recurred HCCs after RFA state were evaluated about the brightness compared with adjacent RFA ablation area. RESULTS:In the 38 patients with HCCs,20 (52.6%)were brighter than surrounding cirrhotic parenchyma. Another 13 (34.2%) were darker. The others (5 cases,13.2%) were seen as the same color as the adjacent liver parenchyma. Post-RFA lesions were darker than previous tumor and surrounding parenchyma in all 38 cases. However,recurred HCCs were brighter than the treated site in all 8 cases. CONCLUSION:Using ARFI technique is helpful for differential diagnosis in order to detect recurred HCCs more easily in patients with confusing status.

关键词： Hepatocellular carcinoma Elastography Radiofrequency ablation

cholecystectomy increases the risk of metabolic syndrome in the Korean population:a longitudinal cohort study

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Hepatobiliary Surgery and Nutrition 2023年第4期12卷 523-533,I0023页

作者： Ji Hye Huh Kyong Joo Lee Yun kyung cho Shinje Moon Yoon Jung Kim kyung-Do Han Jun Goo Kang Seong Jin Lee Sung-Hee Ihm Department of Internal Medicine Hallym University College of MedicineChuncheonRepublic of Korea Department of Internal Medicine Asan Medical CenterUniversity of Ulsan College of MedicineSeoulRepublic of Korea Department of Statistics and Actuarial Science Soongsil UniversitySeoulRepublic of Korea

Background:cholecystectomy is a common surgical procedure to treat symptomatic gallstones;however,the long-term outcomes after cholecystectomy are ***,we aimed to investigate whether incident metabolic syndrome(MetS)is associated with cholecystectomy through a large,population-based,longitudinal ***:Subjects aged≥20 years who underwent cholecystectomy from 2010 to 2014(n=76,485)and controls(n=76,485),matched for age and sex,were identified from the Korean National Health Insurance *** proportional hazards analyses were performed to evaluate the association between cases and incident MetS,and hazard ratios and 95% confidence intervals(CIs)were ***:A total of 152,970 patients were *** age was 52.47±12.76 years,and 50.65% of participants were *** the follow-up period,there were 38,979(25.48%)newly diagnosed MetS cases in the study *** risk of MetS in the cholecystectomy group was approximately 20% higher than that in the control group[adjusted odds ratio(OR),1.20;95%CI:1.17-1.23].In the fully adjusted models,the corresponding ORs for new-onset high waist circumference(WC),low high-density lipoprotein cholesterol(HDL-C)levels,high triglycerides(TG)levels,high blood pressure(BP),and high blood glucose levels were 1.16(1.13-1.19),1.19(1.16-1.22),1.25(1.22-1.28),1.27(1.23-1.31),and 1.21(1.18-1.24),*** was an independent risk factor of incident MetS,after adjusting for potential confounding *** the subgroup analyses,the cholecystectomy group had a higher risk of MetS than the control group in subjects without hypertension or dyslipidemia,***:In this large,population-based study,cholecystectomy was associated with an increased risk of developing MetS,independent of other confounding ***,careful monitoring of metabolic variables and long-term follow-up are required to evaluate MetS risk after cholecystectomy.

关键词： cholecystectomy metabolic syndrome(MetS) obesity diabetes cardiovascular risk

Effect of trapping vascular endothelial growth factor-A in a murine model of dry eye with inflammatory neovascularization

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International Journal of Ophthalmology(English edition) 2016年第11期9卷 1541-1548页

作者： Jin Woo Kwon Jin A choi Eun Young Shin Tae Yoon La Dong Hyun Jee Yeon Woong Chung Yang kyung cho Department of Ophthalmology St.Vincent's HospitalCollege of Medicinethe Catholic University of Korea Research Institute of Medical Science St.Vincent's Hospitalthe Catholic University of Korea

AIM： To evaluate whether trapping vascular endothelial growth factor-A（VEGF-A） would suppress angiogenesis and inflammation in dry eye corneas in a murine corneal suture ***： We established two groups of animals, one with non-dry eyes and the other with induced dry *** both groups, a corneal suture model was used to induce inflammation and neovascularization. Each of two groups was again divided into three subgroups according to the treatment; subgroup I（aflibercept）,subgroup II（dexamethasone） and subgroup III（phosphate buffered saline, PBS）. Corneas were harvested and immunohistochemical staining was performed to compare the extents of neovascularization and CD11b＋ cell infiltration. Real-time polymerase chain reaction was performed to quantify the expression of inflammatory cytokines and VEGF-A in the ***： Trapping VEGF-A with aflibercept resulted in significantly decreased angiogenesis and inflammation compared with the dexamethasone and PBS treatments in the dry eye corneas（all P 〈0.05）, but with no such effects in non-dry eyes. The anti-inflammatory and antiangiogenic effects of VEGF-A trapping were stronger than those of dexamethasone in both dry eye and non-dry eye corneas（all P 〈0.05）. The levels of RNA expression of VEGF-A, TNF-alpha, and IL-6 in the aflibercept subgroup were significantly decreasedcompared with those in the PBS subgroup in the dry eye ***： Compared with non-dry eye corneas,dry eye corneas have greater amounts of inflammation and neovascularization and also have a more robust response to anti-inflammatory and anti-angiogenic agents after ocular surface surgery. Trapping VEGF-A is effective in decreasing both angiogenesis and inflammation in dry eye corneas after ocular surface surgery.

关键词： dry eye aflibercept neovascularization inflammation dexamethasone

Sequence Information on Simple Sequence Repeats and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean

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Journal of Integrative Plant Biology 2011年第1期53卷 63-73页

作者： Kyaw Thu Moe Jong-Wook Chung Young-Il cho Jung-kyung Moon Ja-Hwan Ku Jin-Kyo Jung Jungran Lee Yong-Jin Park Department of Plant Resources College of Industrial SciencesKongju National University The Rural Development Administration(RDA) Legume Bio-Resource Center of Green Manure(LBRCGM) Kongju National University

Mungbean （Vigna radiata （L.） Wilczek） is a unique species in its ability to fix atmospheric nitrogen, with early maturity, and relatively good drought resistance. We used 454 sequencing technology for transcriptome sequencing. A total of 150 159 and 142 993 reads produced 5 254 and 6 374 large contigs （〉_500 bp） with an average length of 833 and 853 for Sunhwa and Jangan, respectively. Functional annotation to known sequences yielded 41.34% and 41.74% unigenes for Jangan and Sunhwa. A higher number of simple sequence repeat （SSR） motifs was identified in Jangan （1 630） compared with that of Sunhwa （1 334）. A similar SSR distribution pattern was observed in both varieties. A total of 8 249 single nucleotide polymorphisms （SNPs） and indels with 2 098 high-confidence candidates were identified in the two mungbean varieties. The average distance between individual SNPs was -860 bp. Our report demonstrates the utility of transcriptomic data for implementing a functional annotation and development of genetic markers. We also provide large resource sequence data for mungbean improvement programs.

关键词： Sequence Information on Simple Sequence Repeats and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean SNPs