Sequence Information on Simple Sequence Repeats and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean

作     者：Kyaw Thu Moe Jong-Wook Chung Young-Il Cho Jung-Kyung Moon Ja-Hwan Ku Jin-Kyo Jung Jungran Lee Yong-Jin Park 

作者机构：Department of Plant ResourcesCollege of Industrial SciencesKongju National University The Rural Development Administration(RDA) Legume Bio-Resource Center of Green Manure(LBRCGM)Kongju National University 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2011年第53卷第1期

页      面：63-73页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：support of the "Cooperative Research Program for Agriculture Science & Technology Development (Project No. 200908FHT020609001)" Rural Development Administration (RDA) Republic of Korea 

主　　题：Sequence Information on Simple Sequence Repeats and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean SNPs 

摘      要：Mungbean （Vigna radiata （L.） Wilczek） is a unique species in its ability to fix atmospheric nitrogen, with early maturity, and relatively good drought resistance. We used 454 sequencing technology for transcriptome sequencing. A total of 150 159 and 142 993 reads produced 5 254 and 6 374 large contigs （〉_500 bp） with an average length of 833 and 853 for Sunhwa and Jangan, respectively. Functional annotation to known sequences yielded 41.34% and 41.74% unigenes for Jangan and Sunhwa. A higher number of simple sequence repeat （SSR） motifs was identified in Jangan （1 630） compared with that of Sunhwa （1 334）. A similar SSR distribution pattern was observed in both varieties. A total of 8 249 single nucleotide polymorphisms （SNPs） and indels with 2 098 high-confidence candidates were identified in the two mungbean varieties. The average distance between individual SNPs was -860 bp. Our report demonstrates the utility of transcriptomic data for implementing a functional annotation and development of genetic markers. We also provide large resource sequence data for mungbean improvement programs.