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Role of Pathogen-Related Protein 10 (PR 10) under Abiotic and Biotic Stresses in Plants

Phyton-International Journal of Experimental Botany 2020年第2期89卷 167-182页

作者： rakesh kumar Sinha Shiv Shankar Verma Anshu Rastogi Department of Plant Biophysics&Biochemistry Biology Center of the Czech Academy of SciencesInstitute of PlantMolecular BiologyCeskeBudejovice37005Czech Republic School of Medicine Case Western Reserve UniversityCleveland44106USA Department of Ecology and Environmental Protection Laboratory of BioclimatologyPoznan University of Life SciencesPoznan60-649Poland

Members of the Pathogenesis Related(PR)10 protein family have been identified in a variety of plant species and a wide range of functions ranging from defense to growth and development has been attributed to ***10 protein possesses ribonuclease(RNase)activity,interacts with phytohormones,involved in hormone-mediated signalling,afforded protection against various phytopathogenic fungi,bacteria,and viruses particularly in response to biotic and abiotic *** resistance mechanism of PR10 protein may include activation of defense signalling pathways through possible interacting proteins involved in mediating responses to pathogens,degradation of RNA of the invading ***,several morphological changes have been shown to accompany the enhanced abiotic stress *** this review,the possible mechanism of action of PR10 protein against biotic and abiotic stress has been ***,our findings also confirmed that the in vivo Nitric oxide(NO)is essential for most of environmental abiotic stresses and disease resistance against pathogen *** proper level of NO may be necessary and beneficial,not only in plant response to the environmental abiotic stress,but also to biotic *** updated information on this interesting group of proteins will be useful in future research to develop multiple stress tolerance in plants.

关键词： Pathogenesis-related(PR) PR10 abiotic stress biotic stress ribonuclease stress tolerance nitric oxide

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Detection of urinary antigens and their seroreactivity with serum of patients in Leishmania donovani infection

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Asian Pacific Journal of Tropical Medicine 2011年第5期4卷 367-370页

作者： Vinod kumar Jalaj kumar Gour Surabhi Bajpai Manish Mishra rakesh kumar Singh Molecular Immunology Laboratory Department of BiochemistryFaculty of ScienceBanaras Hindu UniversityVaranasi-221005India

Objective:To detect leishmanial antigens in pre and post treated urine of visceral leishmaniasis (VL) ***:Urine and serum sample from three VL patients were collected. Ammonium sulphate precipitation and purification of urine sample was done for proteins *** PAGE of proteins was done followed by western blotting,with the patient’s pre and post treatment ***:Eight proteins of molecular weights 17 kDa,25 kDa,28 kDa,42 kDa, 47 kDa,54 kDa,60 kDa and 85 kDa were detected in the urine of VL patients before treatment. After treatment with miltefosine,none of the above proteins was detected in urine *** western blot analysis with pre treatment serum confirmed the antigenicity of four urinary proteins of molecular weights 25 kDa,28 kDa,54 kDa and 60 *** seropositivity with 25 kDa and 28 kDa antigens was negative with serum obtained after the completion of ***:In the context to unavailability of a prognostic tool,urinary leishmanial antigens may offer a better choice and may also be useful as immunoprophylactic candidates.

关键词： Visceral leishmaniasis Urinary antigens Seroreactivity

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D^(2)PAM:Epileptic seizures prediction using adversarial deep dual patch attention mechanism

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CAAI Transactions on Intelligence Technology 2023年第3期8卷 755-769页

作者： Arfat Ahmad Khan rakesh kumar Madendran Usharani Thirunavukkarasu Muhammad Faheem Department of Computer Science College of ComputingKhon Kaen UniversityKhon KaenThailand Department of Computer Science and Engineering Rajalakshmi Engineering CollegeChennaiIndia Department of Biomedical Engineering Saveetha School of EngineeringSaveetha Institute of Medical and Technical Sciences(Deemed to be University)ChennaiIndia School of Technology and Innovations University of VaasaVaasaFinland

Epilepsy is considered as a serious brain disorder in which patients frequently experience *** seizures are defined as the unexpected electrical changes in brain neural activity,which leads to *** researches made an intense effort for predicting the epileptic seizures using brain signal ***,they faced difficulty in obtaining the patients'characteristics because the model's distribution turned to fake predictions,affecting the model's *** addition,the existing prediction models have severe issues,such as overfitting and false positive *** overcome these existing issues,we propose a deep learning approach known as Deep dual‐patch attention mechanism(D^(2)PAM)for classifying the pre‐ictal signals of people with Epilepsy based on the brain *** neural network is integrated with D^(2)PAM,and it lowers the effect of differences between patients to predict *** multi‐network design enhances the trained model's generalisability and stability ***,the proposed model for processing the brain signal is designed to transform the signals into data blocks,which is appropriate for pre‐ictal *** earlier warning of epilepsy with the proposed model obtains the auxiliary *** data of real patients for the experiments provides the improved accuracy by D2PAM approximation compared to the existing *** be more distinctive,the authors have analysed the performance of their work with five patients,and the accuracy comes out to be 95%,97%,99%,99%,and 99%***,the numerical results unveil that the proposed work outperforms the existing models.

关键词： artificial intelligence techniques classification learning(artificial intelligence)

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Strategies to mitigate the adverse effect of drought stress on crop plants-influences of soil bacteria:A review

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Pedosphere 2021年第3期31卷 496-509页

作者： Priyanka CHANDRA Amoolya WUNNAVA Pooja VERMA Amaresh CHANDRA rakesh kumar SHARMA Indian Council of Agricultural Research(ICAR) Central Soil Salinity Research InstituteKarnalHaryana 132001India Department of Biosciences Manipal University JaipurRajasthanJaipur 303007India Indian Council of Agricultural Research(ICAR) Indian Institute of Sugarcane ResearchUttar PradeshLucknow 226002India

Drought stress affects plant growth and causes significant issues in meeting global demand for food crops and *** can cause physiological,physicochemical,and morphological changes in plants,which negatively affects plant growth and *** combat this under the increasing global threat of water shortage and rapid population expansion,it is crucial to develop strategies to meet global food *** growth-promoting rhizobacteria(PGPR)may provide a safe solution to enhancing crop yields through various *** soil bacteria can provide drought tolerance to crop plants,allowing them to survive and thrive in water-scarce *** of phytohormones,free radical-scavenging enzymes,and stress-combating enzymes that can increase tolerance to drought-induced stress are key features of plant-associated microbial *** review summarizes the beneficial properties of microbes that help plants tolerate water scarcity and highlights the bacterial mechanisms that enhance drought tolerance in plants.

关键词： abiotic stress agricultural yield drought tolerance food crops microbe PGPR rhizobactoria water deficit

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An updated study of traditional medicines to the era of 1,3,4 oxadiazole derivatives for malaria treatment

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Traditional Medicine Research 2023年第1期8卷 36-42页

作者： Davinder kumar Harsh kumar Aakash Deep rakesh kumar Marwaha Department of Pharmaceutical Sciences Maharishi Dayanand UniversityRohtak 124001HaryanaIndia Department of Pharmaceutical Sciences Chaudhary Bansi Lal UniversityBhiwani 127021HaryanaIndia.

Discovering potent drug candidates requires new antimalarial *** this reason,antimalarial drug researchers focus on developing new drug candidates on promising *** the identification of these targets,a basic understanding of the metabolism and biochemical processes of malaria parasites such as Plasmodium falciparum can prove ***,malarial resistant parasites are becoming more and more significant issues as antimalarial medications *** drugs are becoming increasingly resistant,thus the search for new molecules is very important *** based combination therapies are becoming more popular for treating *** will ultimately lead to the selection of resistance due to their extensive and indiscriminate *** artemisinin-based combination therapies are therefore *** is an interest in finding suitable antimalarial compounds based on their potential effectiveness against *** potential activity of 1,3,4 oxadiazole analogues against malarial infections is a major draw of the search for suitable antimalarial *** review identifies existing antimalarial drugs that have own therapeutic effects and evaluating their feasibility for developing novel synthetic antimalarial derivatives.

关键词： antimalarial Plasmodium falciparum 1,3,4 oxadiazoles traditional medicines

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Doubled haploid production in advanced back cross generations and molecular cytogenetic characterization of rye chromatin in triticale×wheat derived doubled haploid lines

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BIOCELL 2021年第6期45卷 1651-1659页

作者： MUNIYANDI SAMUEL JEBERSON HARINDER kumar CHAUDHARY rakesh kumar CHAHOTA SHABIR HUSSAIN WANI AICRP(MULLaRP) Directorate of ResearchCAUImphalIndia Molecular Cytogenetics and Tissue Culture Laboratory CSKHPKVPalampurIndia Department of Agricultural Biotechnology CSKHPKVPalampurIndia Mountain Research Center for Field Crops Sher-e-Kashmir University of Agricultural Sciences and TechnologyKhudwaniIndia

The rye genome has shown potential for improvement of bread wheat,where wheat-rye substitutions and translocations have been and are frequently used in resistance *** belongs to different generations viz.,BC_(1)F_(1),BC_(1)F_(2),BC_(1)F_(3),BC_(1)F_(4)and BC_(2)F_(3)of triticale×wheat derived were used for different haploid induction parameters using Gogon grass(Imperata cylindrica)as a pollen *** percentage of pseudo seed formation ranged from 34.55%for BC_(1)F_(2)to 63.77 for BC_(1)F_(1)crosses,the haploid embryo formation ranges from 9.43%for BC_(1)F_(1)to 30.2%for BC_(1)F_(2),the haploid plant generation ranges from 19.36%for BC_(1)F_(2)to 63.25%for BC_(1)F_(1).Four doubled haploids were developed from ITSN 105/58-VL 802-VL 802 of BC_(2)F_(3)underwent molecular cytogenetic analyses using the probes,viz.,rye genomic rDNA,pSc 119 and *** and GISH analysis revealed an IBL.1RS translocation and substitution of 5R chromosome instead of the 5D chromosomes in these doubled haploids.

关键词： Haploid Triticale×wheat backcross FISH GISH Rye chromatin

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Current status and prospects of primary immunodeficiency diseases in Asia

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Genes & Diseases 2020年第1期7卷 3-11页

作者： rakesh kumar Pilania Himanshi Chaudhary Ankur kumar Jindal Amit Rawat Surjit Singh Allergy Immunology Unit Department of PediatricsAdvanced Pediatrics CentrePostgraduate Institute of Medical Education and ResearchChandigarh160012India

Primary Immunodeficiency Diseases(PIDs)are increasingly being reported across the *** advances have been made in the diagnostic and therapeutic research related to *** increasing awareness,the field of PIDs has rapidly evolved in Asia as *** this review,we summarize the progress that has been made in the field of PIDs in Asian countries;major limitations and challenges faced by the clinicians working in this field in Asia;difference in spectrum of PIDs in Asia from rest of the World;current state of diagnostic and treatment facilities available in various countries in Asia and the future prospects of these diseases in the continent.

关键词： Asia Genetic diagnosis Hematopoietic stem cell transplant Intravenous immunoglobulin Primary immunodeficiency diseases

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Emergence of non-albicans Candida species and antifungal resistance in intensive care unit patients

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Asian Pacific Journal of Tropical Biomedicine 2016年第5期6卷 455-460页

作者： Ravinder Kaur Megh Singh Dhakad Ritu Goyal rakesh kumar Department of Microbiology Lady Hardinge Medical College and Associated Hospitals Department of Microbiology Maulana Azad Medical College and Associated Lok Nayak Hospitals Department of Obstetrics and Gynaecology Maulana Azad Medical College and Associated Lok Nayak Hospitals Department of Anaesthesiology Maulana Azad Medical College and Associated Lok Nayak Hospitals

Objective: To evaluate the epidemiology of candidiasis and the antifungal susceptibility profile of Candida species isolated from the intensive care unit(ICU) ***: The study used a qualitative descriptive design. Relevant samples depending on organ system involvement from 100 ICU patients were collected and *** and speciation of the isolates was conducted by the biochemical *** susceptibility testing was carried out as per CLSI-M27-A3 ***: Ninety Candida isolates were isolated from the different clinical samples: urine(43.3%), tracheal aspirate(31.1%), urinary catheter(12.2%), endotracheal tube(7.8%),abdominal drains(3.3%), sputum(2.2%). The incidence of candidiasis caused by nonalbicans Candida(NAC) species(63.3%) was higher than Candida albicans(36.7%).The various NAC species were isolated as: Candida tropicalis(41.1%), Candida glabrata(10%), Candida parapsilosis(6.7%), Candida krusei(3.3%) and Candida kefyr(2.2%). The overall isolation rate of Candida species from samples was 53.3%. Antifungal susceptibility indicated that 37.8% and 7.8% of the Candida isolates were resistant to fluconazole and amphotericin B, ***: Predominance of NAC species in ICU patients along with the increasing resistance being recorded to fluconazole which has a major bearing on the morbidity and management of these patients and needs to be further worked upon.

关键词： Antifungal resistance Candida species Colonization Intensive care unit patients

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Recent advances on pathogenesis,diagnosis,prevention,immunological aspects,and vectors of dengue:A review

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Asian Pacific Journal of Tropical Biomedicine 2023年第8期13卷 325-338页

作者： Mahesh Chandra Sahu rakesh kumar Samantaray Aditi Pal Sanghamitra Pati Division of Medical Entomology ICMR-Regional Medical Research CentreChandrasekharpurBhubaneswar-751023OdishaIndia ICMR-Regional Medical Research Centre ChandrasekharpurBhubaneswar-751023OdishaIndia

Dengue is a vector-borne disease caused by the dengue virus(DENV)of family *** fever is common in both developed and developing ***,approximately 400 million cases of dengue fever are reported annually,resulting in approximately 22000 *** cases in India have progressively increased in the last *** recent years,substantial progress has been made in understanding various aspects of dengue,including its pathogenesis,diagnosis,prevention strategies,immunological responses,and the role of vectors in its *** transmission of a positive RNA virus occurs through Aedes mosquitoes,specifically Aedes aegypti and Aedes *** virus is associated with a wide spectrum of symptoms,ranging from mild undifferentiated fever to severe hemorrhagic fever and shock,posing a potential threat to human *** are 4 types of antigenically distinct dengue serotypes(DENV-1 to DENV-4)and among them,DENV-2 is more lethal and extremely *** overcome the severity of dengue,Dengvaxia is administered to children 9 to 16 years old with evidence of previous dengue *** diagnosis of dengue is carried out by ELISA-based non-structural protein(NS1)and immunoglobulin ***,there are no specific biomarkers to identify severe disease *** factors and temperature play an important role in complex interaction among host,vector,and virus to manifest the severity of *** is a need for the refinement of climate-based disease forecasting models in India to effectively control the spread of *** mosquito repellent should be used periodically to kill or repel the Aedes mosquito to prevent the spread of dengue in humans.

关键词： Dengue Aedes mosquitoes Insecticide NS1 Immunoglobulin

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Thermostable Broad Band Polarizing PVA-Film: Theoretical and Experimental Investigations

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Chinese Journal of Structural Chemistry 2018年第2期37卷 186-197页

作者： SIYAMAK Shahab LIUDMILA Filippovich HORA A.Almodarresiyeh MASOOME Sheikhi rakesh kumar Institute of Physical Organic Chemistry National Academy of Sciences of Belarus Institute of Chemistry of New Materials National Academy of Sciences of Belarus Belarussian State University ISEI BSU Minsk Young Researchers and Elite Club Gorgan Branch Islamic Azad University Department of Chemistry DAV University

In the present work, for the first time on the basis ofpoly （vinyl alcohol）（PVA）, 2- （4-dimethylaminostyryl）-l-ethylquinolinium iodide （quinaldine red （QR）） and trisodium （4E）-5-oxo- 1-（4-sulfonatophenyl）-4-[（4-sulfonatophenyl）hydrazono]-3 pyrazolecarboxylate （tartrazine （T））, thermostable polarizing film in a wide range of spectra （λmax=394-511 nm） with polarization efficiency （PE） = 98% in absorption maximum and stretching degree （Rs） = 3.5 was developed. The basic spectral-polarization parameters （polarization efficiency and transmittance） of oriented colored PVA-films were measured and discussed. During the work it was found that oriented PVA-films are the phenomenon of anisotropy of thermal conductivity （λ｜/λ⊥）. It is a very important parameter for the development of thermostable PVA-polarizing films. For the first time quantum-chemical calculations using density functional theory （DFT） approach for structural analysis and electronic spectrum of the QR were carried out via the B3LYP/dgdzvp and TDB3LYP/dgdzvp methods. Interpretation of absorption strips in visible region of spectrum was also reported. The excitation energies, electronic transitions and oscillator strengths for the studied structures have also been calculated （B3LYP/dgdzvp）. The NBO analysis and Mulliken atomic charges of the QR were carried out.

关键词： thermostable broad band polarizer fdm Quinaldine Red Tartrazine electronicspectrum anisotropy of thermal conductivity

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