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Doubled haploid production in advanced back cross generations and molecular cytogenetic characterization of rye chromatin in triticale×wheat derived doubled haploid lines

作     者:MUNIYANDI SAMUEL JEBERSON HARINDER KUMAR CHAUDHARY RAKESH KUMAR CHAHOTA SHABIR HUSSAIN WANI 

作者机构:AICRP(MULLaRP)Directorate of ResearchCAUImphalIndia Molecular Cytogenetics and Tissue Culture LaboratoryCSKHPKVPalampurIndia Department of Agricultural BiotechnologyCSKHPKVPalampurIndia Mountain Research Center for Field CropsSher-e-Kashmir University of Agricultural Sciences and TechnologyKhudwaniIndia 

出 版 物:《BIOCELL》 (生物细胞(英文))

年 卷 期:2021年第45卷第6期

页      面:1651-1659页

核心收录:

学科分类:09[农学] 0901[农学-作物学] 

主  题:Haploid Triticale×wheat backcross FISH GISH Rye chromatin 

摘      要:The rye genome has shown potential for improvement of bread wheat,where wheat-rye substitutions and translocations have been and are frequently used in resistance *** belongs to different generations viz.,BC_(1)F_(1),BC_(1)F_(2),BC_(1)F_(3),BC_(1)F_(4)and BC_(2)F_(3)of triticale×wheat derived were used for different haploid induction parameters using Gogon grass(Imperata cylindrica)as a pollen *** percentage of pseudo seed formation ranged from 34.55%for BC_(1)F_(2)to 63.77 for BC_(1)F_(1)crosses,the haploid embryo formation ranges from 9.43%for BC_(1)F_(1)to 30.2%for BC_(1)F_(2),the haploid plant generation ranges from 19.36%for BC_(1)F_(2)to 63.25%for BC_(1)F_(1).Four doubled haploids were developed from ITSN 105/58-VL 802-VL 802 of BC_(2)F_(3)underwent molecular cytogenetic analyses using the probes,viz.,rye genomic rDNA,pSc 119 and *** and GISH analysis revealed an IBL.1RS translocation and substitution of 5R chromosome instead of the 5D chromosomes in these doubled haploids.

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