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Enamel Matrix Derivative Stimulates Osteogenesis-and Chondrogenesis-related Transcription Factors in C3H10T1/2 Cells

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Acta Biochimica et Biophysica Sinica 2007年第1期39卷 1-7页

作者： Masataka NARUKAWA Naoto SUZUKI Tadahiro TAKAYAMA Yasuhiko YAMASHITA Kichibee OTSUKA koichi ito Department of Periodontology Nihon University School of DentistryTokyo101-8310Japan Department of Biochemistry Nihon University School of DentustryTokyo 101-8310Japan Division of Functional Morphology Dental Research CenterNihon University School of DentistryTokyo 101-8310Japan Department of Biochemistry Nihon University School of DentistryTokyo101-8310Japan Division of Functional Morphology Dental Research CenterNihon University School of DentistryTokyo101-8310Japan Division of Advanced Dental Treatment Dental Research CenterNihon University School of DentistryTokyo101-8310Japan

Our purpose was to determine how enamel matrix derivative (EMD) affects the expression ofosteogenesis- and chondrogenesis-related transcription factors in undifferentiated mesenchymal cells.C3H10T1/2 cell line,a typical pluripotential mesenchymal cell line,was cultured with or without EMD forup to 7 *** of mRNAs encoding osteogenesis-and chondrogenesis-related transcription factors(Runx2, Osterix,AJ18,Dlx5,Msx2,Sox5,Sox9 and Zfp60) was measured using real-time polymerasechain ***2 and Sox9 protein expression and the presence of bone morphogenetic protein (BMP)-6-like molecules in EMD were determined by Western *** substantially increased mRNA levels ofosteogenesis-and chondrogenesis-related transcription *** also induced Runx2 and Sox9 *** blotting analysis of EMD using anti-BMP-6 antibody revealed immunoreactive bandscorresponding to about 14 kDa and 60 *** results suggest that EMD stimulates osteogenesis- andchondrogenesis-related transcription factors,and these activities may be mediated,at least in part,by BMP-6in EMD.

关键词： enamel matrix derivative osteogenesis chondrogenesis

Microarray Analysis of the Developing Rat Mandible

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Acta Biochimica et Biophysica Sinica 2007年第6期39卷 399-405页

作者： Hideo KABURAGI Naoyuki SUGANO Maiko OSHIKAWA Ryosuke KOSHI Naoki SENDA Kazuhiro KAWAMOTO koichi ito Department of Periodontology. Nihon University School of Dentistry Tokyo 101-8310 Japan Department of Periodontology. Nihon University School of Dentistry Tokyo 101-8310 Japan Division of Advanced Dental Treatment Dental Research Center Nihon University School of Dentistry Tokyo 101-8310 Japan Department of Periodontology. Nihon University School of Dentistry Tokyo 101-8310 JapanDivision of Advanced Dental Treatment Dental Research Center

To analyze the molecular events that occur in the developing mandible, we examined the expression of 8803 genes from samples taken at different time points during rat postnatal mandible development. Total RNA was extracted from the mandibles of 1-day-old, 1-week-old, and 2-week-old rats. Complementary RNA (cRNA) was synthesized from cDNA and biotinylated. Fragmented cRNA was hybridized to RGU34A GeneChip arrays. Among the 8803 genes tested, 4344 were detectable. We identified 148 genes with significantly increased expression, and 19 genes with significantly decreased expression. A comprehensive analysis appears to be an effective method of studying the complex process of development.

关键词： gene expression microarray rat mandible

A New Nonlinear Shell Element Taking the Normal Stress in Thickness Direction into Account for Simulation of Sheet Metal Forming

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A New Nonlinear Shell Element Taking the Normal Stress in Th...

LOCALIZED HEATING BY THE COAXIAL-DIPOLE ANTENNA FOR MICROWAVE COAGULATION THERAPY

The Sixth World Congress on Computational Mechanics in Conjunction with the Second Asian-Pacific Congress on Computational Mechanics

作者： koichi ito koichi Kazama Y.Nagai Graduate School of Information Science Tohoku University 04 Aramaki-Aoba Aoba-ku Sendai 980-8589 Japan Press Kogyo Co. Ltd Engineering Department 2003-1 Endo Fujisawa Kanagawa Japan Press Kogyo Co. Ltd Engineering Department 2003-1 Endo Fujisawa Kanagawa Japan

In simulation of sheet metal forming, the prediction of springback, which is a major defect, is not accurate. One reason for this is that the distributions of internal stresses are not predicted accurately. The conventional degenerated shell elements based on Mindlin-Reissner's theory of plates are usually used to simulate sheet metal forming process. The degenerated sh ell element uses the assumption that the thickness of the element remains constant and the normal stress in thickness direction is zero. These lead to problems in simulation of sheet metal forming, especially in prediction of springback. In this paper, we present a new nonlinear shell element that takes the normal stress in thickness direction into account which allows for large deformation. Two pseudo nodes, which have only one degree of freedom, are introduced on the top surface and bottom surface respectively. As a result, the normal strain in thickness direction can be calculated and the normal stress in thickness direction can be calculated using constitutive relation directly. It's possible to treat the boundary condition that pressure acts on top surfaces and bottom surface is simultaneously constrained. The element is tested in several numerical examples. The simulation results show that stress prediction is improved. The normal stress in thickness direction exhibit agreement with that calculated by surface loads and bending moment decreases as normal stress in thickness direction increases, which indicates that the element can simulate the effect of bottoming, i.e. sheet metal is stamped with a great vertical load after the punch reaches dead point in the process of V-type bending to reduce springback.

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THE SAR ESTIMATION BASED ON THE THERMOGRAPHIC METHOD IN 3-6GHZ RANGE

2000年第五届国际天线、电波传播与电磁理论学术会议

作者： Kazuyuki SAito Sumie HOSAKA Yoshihiko HAYASHI Hiroyuki YOSHIMURA koichi ito Graduate School of Science and Technology Chiba University Japan Graduate School of Science and Technology Chiba University Japan Graduate School of Science and Technology Chiba University Japan Faculty of Engineering Chiba University Japan Faculty of Engineering Chiba University Japan

＜正＞ *** In recent years, various types of applications of electromagnetic techniques to microwave thermal therapy have been developed[1],[2]. The authors have been studying the thin coaxial antennas for the minimally invasive microwave thermal therapy, particularly, interstitial microwave hyperthermia[3] and microwave coagulation therapy （MCT）[4]. In this study, we have investigated the antennas for the MCT. The MCT has been used mainly for the treatment of hepatocellular carcinoma[5]. In

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THE SAR ESTIMATION BASED ON THE THERMOGRAPHIC METHOD IN 3-6G...

2004无线电科学国际会议

作者： Teruo Onishi Ryo Ishido Takuya Takimoto Kazuyuki Saito Shinji Uebayashi Masaharu Takahashi koichi ito NTT DoCoMo Inc. 3-5 Hikarinooka Yokosuka 239-8536 JapanGraduate School of Science and Technology Graduate School of Science and Technology Graduate School of Science and Technology Research Center for Frontier Medical Engineering Chiba University1-33 Yayoi-cho Inage-ku Chiba 263-8522 Japan NTT DoCoMo Inc. 3-5 Hikarinooka Yokosuka 239-8536 Japan Research Center for Frontier Medical Engineering Chiba University1-33 Yayoi-cho Inage-ku Chiba 263-8522 Japan Research Center for Frontier Medical Engineering Chiba University1-33 Yayoi-cho Inage-ku Chiba 263-8522 Japan

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中国北方汉族人HLA—DR的DNA分型

中华微生物学和免疫学杂志 1993年第1期13卷 43-46页

作者：陈香美伊藤巧一解放军总医院肾内科北京日本北里大学移植免疫教室

本研究采用聚合酶链反应技术（PCR）扩增DNA片段，利用30种针对HLA—DR不同等位基因DNA的序列特异性寡核苷酸（SSO）探针检测了255例随机中国北方汉族健康人的HLA—DR亚型的基因频率，并与日本人HLA—DR基因频率进行了比较。该检测方法... 详细信息

本研究采用聚合酶链反应技术（PCR）扩增DNA片段，利用30种针对HLA—DR不同等位基因DNA的序列特异性寡核苷酸（SSO）探针检测了255例随机中国北方汉族健康人的HLA—DR亚型的基因频率，并与日本人HLA—DR基因频率进行了比较。该检测方法用血量少，特异性强，SSO作为一种分型制剂可长期使用，从而为临床上应用此技术检测与疾病相关的HLA—DR抗原提供了依据。

关键词： HLA-DR 寡核苷酸聚合酶链反应

Periodical shedding of cloud cavitation from a single hydrofoil in high-speed cryogenic channel flow

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Journal of Thermal Science 2009年第1期18卷 58-64页

作者： Yutaka ito koichi SETO Takao NAGASAKI Tokyo Institute of Technology

In order to explain criteria for periodical shedding of the cloud cavitation, flow patterns of cavitation around a piano-convex hydrofoil were observed using a cryogenic cavitation tunnel of a blowdown type. Two hydrofoils of similarity of 20 and 60 mm in chord length with two test sections of 20 and 60 mm in width were prepared. Working fluids were water at ambient temperature, hot water and liquid nitrogen. The parameter range was varied between 0.3 and 1.4 for cavitation number, 9 and 17 m/sec for inlet flow velocity, and -8° and 8° for the flow incidence angle, respectively. At incidence angle 8°, that is, the convex surface being suction surface, periodical shedding of the whole cloud cavitation was observed on the convex surface under the specific condition with cavitation number and inlet flow velocity, respectively, 0.5, 9 m/sec for liquid nitrogen at 192℃ and 1.4, 11 m/sec for water at 88℃, whereas under the supercavitation condition, it was not observable. Periodical shedding of cloud cavitation occurs only in the case that there are both the adverse pressure gradient and the slow flow region on the hydrofoil.

关键词： cloud cavitation cryogen visualization hydrofoil

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mPGES-1 expression in non-cancerous liver tissue impacts on postoperative recurrence of HCC

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World Journal of Gastroenterology 2010年第38期16卷 4846-4853页

作者： koichi Nonaka Hikaru Fujioka Yasushi Takii Seigo Abiru Kiyoshi Migita Masahiro ito Takashi Kanematsu Hiromi Ishibashi Clinical Research Center and Department of Surgery National Hospital Organization Nagasaki Medical Center2-1001-1 Kubara Omura 856-8652Japan Department of Hepatology Nagasaki University Graduate School of Biomedical Sciences Department of Transplant and Digestive Surgery Nagasaki University Graduate School of Biomedical Sciences Clinical Research Center and Department of Surgery National Hospital Organization Nagasaki Medical Center2-1001-1 Kubara Omura 856-8652Japan

AIM:To investigate whether microsomal prostaglandin E synthase-1 (mPGES-1) expression in hepatocellular carcinoma (HCC) and in non-cancerous liver affects HCC prognosis after hepatectomy. METHODS: The relationship between patient clinical prof iles, tumor factors, surgical determinants, and mPGES-1 expression and the recurrence-free survival rate were examined in 64 patients who underwent curative hepatectomy between March 2003 and December 2006. RESULTS: The scores for mPGES-1 expression were higher in well differentiated and moderately differentiated HCC tissues than in poorly differentiated HCC tissues (well differentiated, 5.1 ± 2.7; moderately differentiated, 5.1 ± 1.7; poorly differentiated, 3.0 ± 1.8). In noncancerous liver tissues, the mPGES-1 levels were higher in injured liver tissues than in normal tissues. Cirrhotic livers had higher mPGES-1 levels than livers with chronic hepatitis (normal livers, 3.3 ± 0.7; chronic hepatitic livers, 5.4 ± 1.9; cirrhotic livers, 6.4 ± 1.6). A univariate analysis revealed that the recurrence-free survival rate was signif icantly lower in patients with vascular invasion,a higher mPGES-1 level in non-cancerous liver tissue,a larger tumor diameter (≥5 cm), and a lower serum albumin level (≤3.7 g/dL). The mPGES-1 expression in HCC tissues did not correlate well with postoperative recurrence. A multivariate analysis demonstrated that the presence of vascular invasion and higher mPGES-1 levels were statistically significant independent predictors for early postoperative recurrence of ***: Increased mPGES-1 expression in noncancerous liver tissues is closely associated with the early recurrence of HCC after curative resection.

关键词： Curative resection Hepatocellular carcinoma Microsomal prostaglandin E synthase-1 Noncancerous liver tissue Recurrence-free survival

Identification of telomere maintenance gene variations related to lung adenocarcinoma risk by genome-wide association and whole genome sequencing analyses

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Cancer Communications 2024年第2期44卷 287-293页

作者： Kouya Shiraishi Atsushi Takahashi Yukihide Momozawa Yataro Daigo Syuzo Kaneko Takahisa Kawaguchi Hideo Kunitoh Shingo Matsumoto Hidehito Horinouchi Akiteru Goto Takayuki Honda Kimihiro Shimizu Masahiro Torasawa Daisuke Takayanagi Motonobu Saito Akira Saito Yuichiro Ohe Shun-ichi Watanabe koichi Goto Masahiro Tsuboi Katsuya Tsuchihara Sadaaki Takata Tomomi Aoi Atsushi Takano Masashi Kobayashi Yohei Miyagi Kazumi Tanaka Hiroyuki Suzuki Daichi Maeda Takumi Yamaura Maiko Matsuda Yoko Shimada Takaaki Mizuno Hiromi Sakamoto Teruhiko Yoshida Yasushi Goto Tatsuya Yoshida Taiki Yamaji Makoto Sonobe Shinichi Toyooka Kazue Yoneda Katsuhiro Masago Fumihiro Tanaka Megumi Hara Nobuo Fuse Satoshi S.Nishizuka Noriko Motoi Norie Sawada Yuichiro Nishida Kazuki Kumada Kenji Takeuchi Kozo Tanno Yasushi Yatabe Kuniko Sunami Tomoyuki Hishida Yasunari Miyazaki Hidemi ito Mitsuhiro Amemiya Hirohiko Totsuka Haruhiko Nakayama Tomoyuki Yokose Kazuyoshi Ishigaki Toshiteru Nagashima Yoichi Ohtaki Kazuhiro Imai Ken Takasawa Yoshihiro Minamiya Kazuma Kobayashi Kenichi Okubo Kenji Wakai Atsushi Shimizu Masayuki Yamamoto Motoki Iwasaki koichi Matsuda Johji Inazawa Yuichi Shiraishi Hiroyoshi Nishikawa Yoshinori Murakami Michiaki Kubo Fumihiko Matsuda Yoichiro Kamatani Ryuji Hamamoto Keitaro Matsuo Takashi Kohno Division of Genome Biology National Cancer Center Research InstituteTokyoJapan Department of Clinical Genomics National Cancer Center Research InstituteTokyoJapan Laboratory for Statistical Analysis Center for Integrative Medical SciencesRIKENYokohamaJapan Department of Genomic Medicine Research InstituteNational Cerebral and Cardiovascular CenterOsakaJapan Laboratory for Genotyping Development Center for Integrative Medical SciencesRIKENYokohamaJapan Center for Antibody and Vaccine Therapy Research HospitalInstitute of Medical ScienceThe University of TokyoTokyoJapan Department of Medical Oncology and Cancer Center and Center for Advanced Medicine against CancerShiga University of Medical ScienceOtsuJapan Division of Medical AI Research and Development National Cancer Center Research InstituteTokyoJapan Center for Genomic Medicine Graduate School of MedicineKyoto UniversityKyotoJapan Department of Medical Oncology Japanese Red Cross Medical CenterTokyoJapan Department of Thoracic Oncology National Cancer Center Hospital EastJapan Department of Thoracic Oncology National Cancer Center HospitalTokyoJapan Department of Cellular and Organ Pathology Graduate School of MedicineAkita UniversityAkitaJapan Department of Respiratory Medicine Tokyo Medical and Dental UniversityBunkyo-kuTokyoJapan Division of General Thoracic Surgery Department of SurgeryShinshu University School of MedicineMatsumotoNaganoJapan Department of General Surgical Science Division of General Thoracic SurgeryGunma University Graduate School of MedicineIntegrative Center of General SurgeryGunma University HospitalGunmaJapan Department of Gastrointestinal Tract Surgery Fukushima Medical University School of MedicineFukushimaJapan StaGen Co. LtdTokyoJapan Division of Thoracic Surgery National Cancer Center HospitalTokyoJapan Department of Thoracic Surgery National Cancer Center Hospital EastChibaJapan Division of Translational Informatics Exploratory Oncology Research and Clinical Trial Center(EPOC

Dear editor,Lung carcinoma is responsible for the highest fatal-ity rate among cancer-related deaths globally,with lung adenocarcinoma(LADC)emerging as the prevailing sub-type.

关键词： adenocarcinoma lung Lung