Microarray Analysis of the Developing Rat Mandible

作     者：Hideo KABURAGI Naoyuki SUGANO Maiko OSHIKAWA Ryosuke KOSHI Naoki SENDA Kazuhiro KAWAMOTO Koichi ITO 

作者机构：Department of Periodontology. Nihon University School of Dentistry Tokyo 101-8310 Japan Department of Periodontology. Nihon University School of Dentistry Tokyo 101-8310 Japan Division of Advanced Dental Treatment Dental Research Center Nihon University School of Dentistry Tokyo 101-8310 Japan Department of Periodontology. Nihon University School of Dentistry Tokyo 101-8310 JapanDivision of Advanced Dental Treatment Dental Research Center 

出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))

年 卷 期：2007年第39卷第6期

页      面：399-405页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071002[理学-动物学] 

基　　金：This work was supported by a grant from the Ministry of Education Culture  Sports Science and Technology Japan 

主　　题：gene expression microarray rat mandible 

摘      要：To analyze the molecular events that occur in the developing mandible, we examined the expression of 8803 genes from samples taken at different time points during rat postnatal mandible development. Total RNA was extracted from the mandibles of 1-day-old, 1-week-old, and 2-week-old rats. Complementary RNA (cRNA) was synthesized from cDNA and biotinylated. Fragmented cRNA was hybridized to RGU34A GeneChip arrays. Among the 8803 genes tested, 4344 were detectable. We identified 148 genes with significantly increased expression, and 19 genes with significantly decreased expression. A comprehensive analysis appears to be an effective method of studying the complex process of development.