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Molecular characterization of sandflies and Leishmania detection in main vector of zoonotic cutaneous leishmaniasis in Abarkouh district of Yazd province,Iran

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Asian Pacific Journal of Tropical Medicine 2013年第10期6卷 792-797页

作者： Jafari R Najafzadeh N Sedaghat mm Parvizi P National Institute of Health Research Isfahan Health Research Station Molecular Systematic Laboratory Parasitology DepartmentPasteur Institute of Iran Department of Microbiology Islamic Azad UniversityScience and Research Branch of Fars Department of Medical Entomology and Vector Control School of Public HealthTehran University of Medical Sciences

Ohjective:To assess molecular characterization,distribution,seasonal activities of sandfly species and Leishmania parasites infecting them for this zoonotic cutaneous leishmaniasis ***:The collections were carried out in 2009-2011 using CDC traps,Sticky Papers and manual aspirator in and around the villages in Abarkouh *** sandflies were characterized by PCR amplification and sequencing of fragments of their mitochondrial cytochrome b *** parasite infections within sandflies were performed by targeting Cyt b,ITS-rDNA,k-DNA and microsatellite ***:The PCR assays detected only Leishmania major(***).All infections(30)were found in the abundant and widespread vector Phlebolomus papalasi(***).Small numbers of other sandfly species were also screened for infections,but none was *** sinloni and *** were the predominant members in all locations of this district and in all habitats throughout the trapping *** five other sandfly species were found,namely Phlebolomus ansari,Phlebotomus caucasicus,Phlebotomus sergenti,Sergentomyia dentata and Sergentomyia ***:In the current survey,the only infections detected are of *** in females of ***(30 out of190).The rates of infection of *** by *** are not significantly different in compare with other locations in Iran with no diversity of parasite *** cutaneous leishmaniasis may have emerged only recently in Abarkouh district,and the reason may well be the instability of the transmission cycles there.

关键词： Zoonotic cutaneous leishmaniasis Phlebotomus papatasi Leishmania major Sandfly Central Iran

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Biliary microlithiasis,sludge,crystals, microcrystallization,and usefulness of assessment of nucleation time

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Hepatobiliary & Pancreatic Diseases International 2010年第3期9卷 248-253页

作者： Vasitha Abeysuriya Kemal I Deen Navarathne mm Navarathne Department of Clinical Anatomy National Hospital of Sri Lanka Department of Surgery National Hospital of Sri Lanka Faculty of Medicine University of Kelaniya Department of Gastroenterology National Hospital of Sri Lanka

BACKGROUND:The process of microcrystallization,its sequel and the assessment of nucleation time is *** systematic review aimed to highlight the importance of biliary microlithiasis,sludge,and crystals,and their association with gallstones,unexplained biliary pain,idiopathic pancreatitis, and sphincter of Oddi *** SOURCES:Three reviewers performed a literature search of the PubMed *** words used were'biliary microlithiasis','biliary sludge','bile crystals','cholesterol crystallisation','bile microscopy','microcrystal formation of bile','cholesterol monohydrate crystals','nucleation time of cholesterol','gallstone formation','sphincter of Oddi dysfunction'and'idiopathic pancreatitis'.Additional articles were sourced from references within the studies from the PubMed ***:We found that biliary microcrystals account for almost all patients with gallstone disease,7%to 79%with idiopathic pancreatitis,83%with unexplained biliary pain, and 25%to 60%with altered biliary and pancreatic sphincter ***,the detection of biliary microcrystals in gallstone disease has a sensitivity ranging from 55%to 87%and a specificity of 100%.In idiopathic pancreatitis,the presence of microcrystals ranges from 47%to 90%.A nucleation time less than 10 days in hepatic bile or ultra-filtered gallbladder bile has a specificity of 100%for cholesterol gallstone ***:Biliary crystals are associated with gallstone disease,idiopathic pancreatitis,sphincter of Oddi dysfunction, unexplained biliary pain,and post-cholecystectomy biliary *** of cholesterol super-saturation,crystallisation, and gallstone formation have been described with *** microscopy is a useful method to detect microcrystals and the assessment of nucleation time is a good method of predicting the risk of cholesterol crystallisation.

关键词： KEY WORDS:biliary microlithiasis sludge crystals gallstones idiopathic pancreatitis sphincter of Oddi dysfunction

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Effects of Increasing Tungsten on Structural,Elastic and Optical Properties of xWO_3-(40-x)Ag_2O-60Te_2O Glass System

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Journal of Materials Science & Technology 2015年第1期31卷 83-90页

作者： mm.Umair A.K.Yahya M.K.Halimah H.A.A.Sidek School of Physics and Materials Studies Universiti Teknologi MARA Department of Physics Faculty of ScienceUniversiti Putra Malaysia

Ternary teiiurite glasses with composition of xWO3（40-x）Ag2O-60TeO2（x=0-40 mol%） have been prepared by melt-quenching *** and optical properties of the glass system were obtained by ultrasonic velocity measurements and UV-vis spectroscopy,respectively,while structural investigation was carried out by using Raman *** longitudinal and shear velocities,vLand vsshowed large increase at x from 0 to 20 mol%before decrease with further addition of *** longitudinal modulus CLand shear modulus μ bulk modulus KeYoung’s modulus Vand Debye temperature θDshowed similar behaviors to both *** large increase of the elastic moduli at x from 0 to 20 mol%is suggested to be due to the increase in WO6octahedral unit structure indicating the increase of bridging oxygen（BO） and formation of stronger Te-O-W bonds compared to Te-O-Te *** the other hand,forx> 20 mol%,the decrease in the elastic moduli was due to the increase of non-bridging oxygen（NBO） as a result of formation of WO4tetrahedral via breaking Te-O-W *** analysis by using bulk compression and ring deformation models showed a slight decrease in the ratio of ideal bulk modulus Kbcto the experimental bulk modulus Keand average atomic ring size,/ for× 20 mol%.Our analysis also indicates that limited ring deformation took place and the main compression mechanism in this glass system was mainly ideal isotropic *** the other hand,optical band gap Eoptshowed small variation for x=0-20 mol%but decreased upon higher WO3content while refractive index n showed the opposite *** optical behavior is suggested to be related to the changes in cross link density and NBO concentration in the glass system.

关键词： Elastic moduli Optical absorption Raman spectrosco

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Study on 3D Quantitative Structure-Activity Relationships of Taxoid

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Chinese Chemical Letters 1997年第10期8卷 893-896页

作者： Zhu, QQ Guo, ZR Huang, N Wang, mm Chu, FM CHINESE ACAD MED SCI INST MAT MEDDEPT SYNTH MED CHEMBEIJING 100050PEOPLES R CHINA

A series of 94 taxol analogues exhibiting antitumor activity were investigated using comparative molecular field analysis (CoMFA) method. The most optimal CoMFA from 80 compounds selected randomly yielded a two-components model, with significant cross-validation r(cv)(2) of 0.640 and conventional r(2) of 0.868. The robustness of the CoMFA model was further validated by prediction of 14 test set compounds. The CoMFA model explained present SAR and particularly the importance of the hydroxyl group at C-2' position and the stereochemistry at C-2' and C-3' positions.

关键词： BIOLOGICAL-ACTIVITY ANTITUMOR AGENTS ANALOGS 9(R)-DIHYDROTAXOL CONFORMATION

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Review on Partial Discharge Detection Techniques Related to High Voltage Power Equipment Using Different Sensors

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Photonic Sensors 2014年第4期4卷 325-337页

作者： mm YAACOB MA ALSAEDI JR RASHED AM DAKHIL SF ATYAH Institute of High Voltage and High Current Faculty of Electrical Engineering Universiti Teknologi Malaysia Department of Electrical Engineering College of Engineering Misan University Iraq

当在高电压操作一台设备或一个功率系统时，与部分分泌物(PD ) 联系的问题能被追踪到象臭氧和氧化氩氮气体的形成那样的电磁的排放，声学的排放或化学反应。高电压设备和高电压安装主人与对在象力量变压器，煤气的绝缘的变电所(GIS ) ... 详细信息

当在高电压操作一台设备或一个功率系统时，与部分分泌物(PD ) 联系的问题能被追踪到象臭氧和氧化氩氮气体的形成那样的电磁的排放，声学的排放或化学反应。高电压设备和高电压安装主人与对在象力量变压器，煤气的绝缘的变电所(GIS ) ，和电报安装那样的设备监视 PD 的过程的条件的需要达成协议。这份报纸考察象电的察觉，化学察觉，声学的察觉，和光察觉那样的可得到的 PD 察觉方法(包含的高电压设备) 。每个方法的优点和劣势被探索了并且比较。评论建议光察觉技术为应用在精确性和适用性的考虑提供许多优点什么时候与另外的技术相比。

关键词：检测技术设备使用局部放电电力系统气体绝缘变电站传感器高电压设备高压

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Induction of apoptosis by arsenic trioxide and hydroxycamptothecin in gastric cancer cells in vitro

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World Journal of Gastroenterology 2000年第4期6卷 532-539页

作者： Tu SP Zhong J Tan JH Jiang XH Qiao mm Wu YX Jiang SH Shanghai Second Med Univ Ruijin Hosp Dept Gastroenterol Shanghai 200025 Peoples R China

AIM To study the effects of arsenic trioxide andHCPT on different degrees of differentiated gastriccancer cells（SGC-7901,MKN-45,MKN-28）withrespect to both cytotoxicity and induction ofapoptosis in *** The cytotoxicity of As2O3 and HCPTon gastric cancer cells was determined by *** changes of apoptosis ofgastric cancer cells were observed by lightmicroscopy and transmission electron *** and cell cycle changes of gastric cancercells induced by HCPT and As2O3 were investigatedby TUNEL method and flow *** As2O3 and HCPT had remarkablecytotoxic effects on different degrees ofdifferentiated gastric cancer *** IC50ofAs2O3 on well differentiated gastric cancer cellMKN-28,moderately differentiated gastric cancercell SGC-7901,and poorly differentiated gastriccancer cell MKN-28 were 8.91 μmol/L,10.57μmol/L,and 11.65 μmol/L,*** IC50of HCPT on MKN-28,SGC-7901,and MKN-45 were9.35 mg/L,10.21 mg/L,and 12.63 mg/Lrespectively after 48 h *** 12 h ofexposure to both drugs,gastric cancer cellsexhibited morphologic features of apoptosis,including cell shrinkage,nuclear condensation, and formation of apoptotic bodies.A typicalsubdiploid peak before G0/G1 phase was observedby flow *** apoptotic rates of SGC-7901,MKN-45,and MKN-28 were 13.84%,22.52%,and 9.68%,respectively after 48 hexposure to 10 μmol/L As2O3.The apoptotic ratesof SGC-7901,MKN-45,and MKN-28 were 21.88%,12.35%,and 30.26%,respectively after 48 hexposure to 10 mg/L *** apoptotic indicewere 7%-15% as assessed by TUNEL *** effect of As2O3 on SGC-7901 showedremarkable cell cycle specificity,which inducedcell death in G1 phase,and blocked G2/M *** also showed a remarkable cell cyclespecificity,by inducing cell death and a

关键词： gastric cancer apoptosis arsenic trioxide hydroxycamptothecin

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A case report of pulmonary coinfection of Strongyloides stercoralis and Pneumocystis jiroveci

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Asian Pacific Journal of Tropical Biomedicine 2011年第4期1卷 334-336页

作者： Bava AJ Romero mm Prieto R Troncoso A Laboratory of Parasitology Infectious Diseases HospitalBuenos AiresArgentina Respiratory Unit of Intensive Care Infectious Diseases HospitalBuenos AiresArgentina Department of Microbiology ParasitologyInfectious Diseases and Tropical MedicineSchool of MedicineBuenos Aires UniversityArgentina

A case of pulmonary coinfection by Strongyloides stercoralis and Pneumocystis jiroveci has been detected in an AIDS patient treated in the Respiratory Intensive Care Unit of the Muniz *** diagnosis,the patient presented cough with mucopurulent expectoration,dyspnea,fever,bilateral pulmonary infiltrates on the chest X-ray,negative bacilloscopy for acid fast bacteria and a CD4^+ T lymphocytes count of 52 cells/μ *** microbiological diagnosis was achieved by microscopic observation of the respiratory secretions obtained by bronchoalveolar lavage,while the wet mount examination revealed rhabditiform and filariform larvae of the nematode and foamy exudates,pathognomonic of the pulmonary *** was the unique case of this association among about 3 000 samples performed in our laboratory in the last 10 years and diagnosed by *** complementary stains(a rapid modification of Grocott,Kinyoun and Giemsa) were applied to the smears after the diagnosis of mycotic and parasitary infections achieved by fresh *** physicians and microbiologists should take into account the possible coexistence of respiratory pathogens in immunocompromised patients,such as those with AIDS.

关键词： Strongyloides stercoralis Pneumocystis jiroveci Pulmonary coinfection AIDS Bronchoalveolar lavage Wet mout examination Pulmonary pneumocystosis

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Studies on the seasonal variations in the proximate composition of ascidians from the Palk Bay,Southeast coast of India

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Asian Pacific Journal of Tropical Biomedicine 2012年第10期2卷 793-797页

作者： Ananthan G Karthikeyan mm Selva Prabhu A Raghunathan C Centre of Advanced Study in Marine Biology Faculty of Marine SciencesAnnamalai University Zoological Survey of India Andaman and NicobarRegional Station.1Horticulture RoadHaddoPort Blair744102

Objective:To investigate the seasonal fluctuations of the proximate composition of the ascidians ***:The moisture content was estimated by drying 1 g of fresh tissue at a constant temperature at 105(?)for 24 *** loss of weight was taken as moisture *** total protein was estimated using the Biuret *** total carbohydrate in dried sample was estimated spectrophotometrically following the phenol-sulphuric acid *** lipid in the dried sample tissue was gravimetrically estimated following the chloroform-methanol mixture *** content was determined gravimetrically by incinerating 1 g dried sample in muffle furnace at about 550℃for 6 h and results are expressed in ***:It was found very difficult to compare the monthly variations,as all the ten species,exhibited wide fluctuations in their proximate *** the sake of convenience,average seasonal values were calculated by summing the monthly ***:The proximate composition of the 10 commonly available ascidians showed high nutritive value and hence these groups especially solitary ascidians can be recommended for human consumption in terms of pickles,soup,curry and others after ensuring the safety of consumers.

关键词： Ascidians Biochemical composition Seasonal variations proximate composition

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Gastroenterology in developing countries:Issues and advances

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World Journal of Gastroenterology 2009年第23期15卷 2839-2854页

作者： Kate L Mandeville Justus Krabshuis Nimzing Gwamzhi Ladep Chris JJ Mulder Eamonn mm Quigley Shahid A Khan Centre for Infectious Diseases Epidemiology Department of Primary Care and Population Sciences University College London Hampstead Campus Royal Free Hospital London NW3 2PF United Kingdom Highland Data Les Charleix 24390 Tourtoirac Dordogne France Department of Medicine University of Jos and Jos University Teaching Hospital Jos Plateau State P.M.B. 2076 Nigeria Department of Gastroenterology VU University Medical Center Amsterdam 1081 HV Holland World Gastroenterology Organisation and Department of Medicine National University of Ireland Cork University Hospital Clinical Sciences Building Wilton Cork Ireland Department of Hepatology and Gastro-enterology Faculty of Medicine Imperial College London St Mary's Campus London W2 1NY United Kingdom

Developing countries shoulder a considerable burden of gastroenterological disease. Infectious diseases in particular cause enormous morbidity and mortality. Diseases which afflict both western and developing countries are often seen in more florid forms in poorer countries. Innovative techniques continuously improve and update gastroenterological practice. However, advances in diagnosis and treatment which are commonplace in the West, have yet to reach many developing countries. Clinical guidelines, based on these advances and collated in resource-rich environments,lose their relevance outside these settings. In this two-part review, we first highlight the global burden of gastroenterological disease in three major areas: diarrhoeal diseases, hepatitis B, and Helicobacter pylori . Recent progress in their management is explored, with consideration of future solutions. The second part of the review focuses on the delivery of clinical services in developing countries. Inadequate numbers of healthcare workers hamper efforts to combat gastroenterological disease. Reasons for this shortage are examined, along with possibilities for increased specialist training. Endoscopy services, the mainstay of gastroenterology in the West, are in their infancy in many developing countries. The challenges faced by those setting up a service are illustrated by the example of a Nigerian endoscopy unit. Finally, we highlight the limited scope of many clinical guidelines produced in western countries. Guidelines which take account of resource limitations in the form of "cascades" are advocated in order to make these guidelines truly global. Recognition of the different working conditions facing practitioners worldwide is an important step towards narrowing the gap between gastroenterology in rich and poor countries.

关键词：发展中国家胃肠病传染性疾病预防措施

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Effect of epidermal growth factor on buffalo frozen spermatozoa biometry and metabolic activity

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Asian pacific Journal of Reproduction 2017年第1期6卷 46-51页

作者： Mohamed mm Kandiel Ahmed RM El-Khawagah Karima Gh Mahmoud Department of Theriogenology Faculty of Veterinary MedicineBenha UniversityEgypt Department of Animal Reproduction and Artificial Insemination National Research CentreDokkiGizaEgypt

Objective:To assess the effects of epidermal growth factor (EGF) on the Egyptian buffalo bull frozen semen, EGF was incorporated at 0 (control), 50, 100, 200 and 400 ng/mL of extender (Bioxcell?).Methods:Semen features, spermatozoa biometry, total liberated amounts of enzymes (aspartate transaminase, alanine aminotransferase, lactate dehydrogenase, acid phosphatase and alkaline phosphatase) and lipid peroxidation markers (thiobarbituric acid reactive substances, malondialdehyde, glutathione peroxidase, nitric oxide, catalase (CAT) and superoxide dismutase (SOD)) were determined in the spermatozoa-free extracellular extender. Results:Spermatozoa membrane integrity significantly (P<0.05) increased, but DNA integrity decreased with EGF 200 ng/mL. Spermatozoa head (dimensions, area and perimeter), but not shape, as well as acrosome and midpiece measures substantially differed with regard to EGF. Principle piece length and volume markedly decreased (at 100 and 200 ng/mL), while total tail/flagellum length increased (at 50 ng/mL) after EGF supplementation. EGF 50 ng/mL was associated with the decline of nitric oxide levels and catalase enzyme activity, but EGF 100 ng/mL significantly decreased the total liberated amounts of enzymes (aspartate transaminase, lactate dehydrogenase, acid phosphatase and alkaline phosphatase) as well as lipid peroxidation markers (thiobarbituric acid reactive substances and malondialdehyde).Conclusion:EGFin vitro supplementation would affect the semen characteristics of buffalo bull with 100 ng/mL counteracted the freezing mediated oxidative stress indicated with the lowest enzymes leakage and lipid peroxidation.

关键词： Buffalo Epidermal growth factor Lipid peroxidation Seminal enzymes Speriogram

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