Molecular characterization of sandflies and Leishmania detection in main vector of zoonotic cutaneous leishmaniasis in Abarkouh district of Yazd province,Iran

作     者：Jafari R Najafzadeh N Sedaghat MM Parvizi P 

作者机构：National Institute of Health ResearchIsfahan Health Research Station Molecular Systematic LaboratoryParasitology DepartmentPasteur Institute of Iran Department of MicrobiologyIslamic Azad UniversityScience and Research Branch of Fars Department of Medical Entomology and Vector ControlSchool of Public HealthTehran University of Medical Sciences 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2013年第6卷第10期

页      面：792-797页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the Pasteur Institute of Iran grant 501 awarded to Dr.Parviz Parvizi,Another part of this wok was funded by National Institute Of Health Research (NIHR) project No 63-9024 

主　　题：Zoonotic cutaneous leishmaniasis Phlebotomus papatasi Leishmania major Sandfly Central Iran 

摘      要：Ohjective:To assess molecular characterization,distribution,seasonal activities of sandfly species and Leishmania parasites infecting them for this zoonotic cutaneous leishmaniasis ***:The collections were carried out in 2009-2011 using CDC traps,Sticky Papers and manual aspirator in and around the villages in Abarkouh *** sandflies were characterized by PCR amplification and sequencing of fragments of their mitochondrial cytochrome b *** parasite infections within sandflies were performed by targeting Cyt b,ITS-rDNA,k-DNA and microsatellite ***:The PCR assays detected only Leishmania major(***).All infections(30)were found in the abundant and widespread vector Phlebolomus papalasi(***).Small numbers of other sandfly species were also screened for infections,but none was *** sinloni and *** were the predominant members in all locations of this district and in all habitats throughout the trapping *** five other sandfly species were found,namely Phlebolomus ansari,Phlebotomus caucasicus,Phlebotomus sergenti,Sergentomyia dentata and Sergentomyia ***:In the current survey,the only infections detected are of *** in females of ***(30 out of190).The rates of infection of *** by *** are not significantly different in compare with other locations in Iran with no diversity of parasite *** cutaneous leishmaniasis may have emerged only recently in Abarkouh district,and the reason may well be the instability of the transmission cycles there.